# HG changeset patch # User iuc # Date 1582140420 18000 # Node ID a1f0b3f4b78144ed7b046b6705dab66fd3a9208f # Parent 9ffcddf6f9c00a2daf7f82ad6f392f346c0913ac "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/picard commit 11b94d7cd69b919f16feb10bd4b830a963334ab8" diff -r 9ffcddf6f9c0 -r a1f0b3f4b781 picard_CollectRnaSeqMetrics.xml --- a/picard_CollectRnaSeqMetrics.xml Mon Feb 17 10:24:29 2020 -0500 +++ b/picard_CollectRnaSeqMetrics.xml Wed Feb 19 14:27:00 2020 -0500 @@ -2,7 +2,7 @@ collect metrics about the alignment of RNA to various functional classes of loci in the genome picard_macros.xml - 1 + 2 r-base @@ -22,18 +22,16 @@ ## The awk line below converts a file obtained from UCSC as specified in the tool help to refFlat format #if str($gene_reference_source.gene_reference_source_selector) == "gtf" - #if $gene_reference_source.refFlat.ext != 'gff3' - gtfToGenePred -genePredExt '${gene_reference_source.refFlat}' refFlat.tab.raw && + #if $gene_reference_source.refFlat.ext == 'gff3' + gff3ToGenePred '${gene_reference_source.refFlat}' refFlat.tab.raw && #else - gff3ToGenePred '${gene_reference_source.refFlat}' refFlat.tab.raw && + gtfToGenePred -genePredExt '${gene_reference_source.refFlat}' refFlat.tab.raw && #end if - grep -v '^#' refFlat.tab.raw | awk '{print $12"\t"$1"\t"$2"\t"$3"\t"$4"\t"$5"\t"$6"\t"$7"\t"$8"\t"$9"\t"$10}' > refFlat.tab && #else - grep -v '^#' ${refFlat} | awk '{print $11"\t"$1"\t"$2"\t"$3"\t"$4"\t"$5"\t"$6"\t"$7"\t"$8"\t"$9"\t"$10}' > refFlat.tab && + grep -v '^#' ${refFlat} | awk '{if ($3 == "+" || $3 == "-") print $11"\t"$1"\t"$2"\t"$3"\t"$4"\t"$5"\t"$6"\t"$7"\t"$8"\t"$9"\t"$10; else print}' > refFlat.tab && #end if - ## Start picard command @java_options@ @@ -145,6 +143,20 @@ + + + + + + + + + + + + + + diff -r 9ffcddf6f9c0 -r a1f0b3f4b781 test-data/picard_CollectRnaSeqMetrics.refFlat --- a/test-data/picard_CollectRnaSeqMetrics.refFlat Mon Feb 17 10:24:29 2020 -0500 +++ b/test-data/picard_CollectRnaSeqMetrics.refFlat Wed Feb 19 14:27:00 2020 -0500 @@ -1,21 +1,21 @@ -#hg19.knownGene.name hg19.knownGene.chrom hg19.knownGene.strand hg19.knownGene.txStart hg19.knownGene.txEnd hg19.knownGene.cdsStart hg19.knownGene.cdsEnd hg19.knownGene.exonCount hg19.knownGene.exonStarts hg19.knownGene.exonEnds hg19.kgXref.kgID -uc004coq.4 chrM - 235 368 235 235 1 235, 368, uc004coq.4 -uc022bqo.2 chrM + 650 674 650 650 1 650, 674, uc022bqo.2 -uc004cor.1 chrM + 1603 1634 1603 1603 1 1603, 1634, uc004cor.1 -uc004cos.5 chrM + 1843 4264 1843 1843 1 1843, 4264, uc004cos.5 -uc022bqp.1 chrM - 5543 5566 5543 5543 1 5543, 5566, uc022bqp.1 -uc022bqq.1 chrM - 5585 5606 5585 5585 1 5585, 5606, uc022bqq.1 -uc022bqr.1 chrM - 5690 5714 5690 5690 1 5690, 5714, uc022bqr.1 -uc031tga.1 chrM + 5904 7439 5904 5904 1 5904, 7439, uc031tga.1 -uc022bqs.1 chrM - 7586 15888 7586 7586 2 7586,15503, 7982,15888, uc022bqs.1 -uc011mfi.2 chrM + 7586 9208 7586 7586 1 7586, 9208, uc011mfi.2 -uc022bqt.1 chrM - 8366 14149 8366 8366 2 8366,13449, 8472,14149, uc022bqt.1 -uc022bqu.2 chrM + 10059 10404 10059 10059 1 10059, 10404, uc022bqu.2 -uc004cov.5 chrM + 10470 12138 10470 10470 1 10470, 12138, uc004cov.5 -uc031tgb.1 chrM - 10760 14149 10760 10760 2 10760,13978, 11231,14149, uc031tgb.1 -uc004cow.2 chrM + 12207 12264 12207 12207 1 12207, 12264, uc004cow.2 -uc004cox.4 chrM + 12907 14149 12907 12907 1 12907, 14149, uc004cox.4 -uc022bqv.1 chrM - 14674 14698 14674 14674 1 14674, 14698, uc022bqv.1 -uc022bqw.1 chrM + 14856 15888 14856 14856 1 14856, 15888, uc022bqw.1 -uc022bqx.1 chrM - 15959 16024 15959 15959 1 15959, 16024, uc022bqx.1 -uc004coz.1 chrM + 15998 16571 15998 15998 1 15998, 16571, uc004coz.1 +#hg19.kgXref.kgID hg19.knownGene.name hg19.knownGene.chrom hg19.knownGene.strand hg19.knownGene.txStart hg19.knownGene.txEnd hg19.knownGene.cdsStart hg19.knownGene.cdsEnd hg19.knownGene.exonCount hg19.knownGene.exonStarts hg19.knownGene.exonEnds +uc004coq.4 uc004coq.4 chrM - 235 368 235 235 1 235, 368, +uc022bqo.2 uc022bqo.2 chrM + 650 674 650 650 1 650, 674, +uc004cor.1 uc004cor.1 chrM + 1603 1634 1603 1603 1 1603, 1634, +uc004cos.5 uc004cos.5 chrM + 1843 4264 1843 1843 1 1843, 4264, +uc022bqp.1 uc022bqp.1 chrM - 5543 5566 5543 5543 1 5543, 5566, +uc022bqq.1 uc022bqq.1 chrM - 5585 5606 5585 5585 1 5585, 5606, +uc022bqr.1 uc022bqr.1 chrM - 5690 5714 5690 5690 1 5690, 5714, +uc031tga.1 uc031tga.1 chrM + 5904 7439 5904 5904 1 5904, 7439, +uc022bqs.1 uc022bqs.1 chrM - 7586 15888 7586 7586 2 7586,15503, 7982,15888, +uc011mfi.2 uc011mfi.2 chrM + 7586 9208 7586 7586 1 7586, 9208, +uc022bqt.1 uc022bqt.1 chrM - 8366 14149 8366 8366 2 8366,13449, 8472,14149, +uc022bqu.2 uc022bqu.2 chrM + 10059 10404 10059 10059 1 10059, 10404, +uc004cov.5 uc004cov.5 chrM + 10470 12138 10470 10470 1 10470, 12138, +uc031tgb.1 uc031tgb.1 chrM - 10760 14149 10760 10760 2 10760,13978, 11231,14149, +uc004cow.2 uc004cow.2 chrM + 12207 12264 12207 12207 1 12207, 12264, +uc004cox.4 uc004cox.4 chrM + 12907 14149 12907 12907 1 12907, 14149, +uc022bqv.1 uc022bqv.1 chrM - 14674 14698 14674 14674 1 14674, 14698, +uc022bqw.1 uc022bqw.1 chrM + 14856 15888 14856 14856 1 14856, 15888, +uc022bqx.1 uc022bqx.1 chrM - 15959 16024 15959 15959 1 15959, 16024, +uc004coz.1 uc004coz.1 chrM + 15998 16571 15998 15998 1 15998, 16571, diff -r 9ffcddf6f9c0 -r a1f0b3f4b781 test-data/picard_CollectRnaSeqMetrics.ucsc_output --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/picard_CollectRnaSeqMetrics.ucsc_output Wed Feb 19 14:27:00 2020 -0500 @@ -0,0 +1,21 @@ +#hg19.knownGene.name hg19.knownGene.chrom hg19.knownGene.strand hg19.knownGene.txStart hg19.knownGene.txEnd hg19.knownGene.cdsStart hg19.knownGene.cdsEnd hg19.knownGene.exonCount hg19.knownGene.exonStarts hg19.knownGene.exonEnds hg19.kgXref.kgID +uc004coq.4 chrM - 235 368 235 235 1 235, 368, uc004coq.4 +uc022bqo.2 chrM + 650 674 650 650 1 650, 674, uc022bqo.2 +uc004cor.1 chrM + 1603 1634 1603 1603 1 1603, 1634, uc004cor.1 +uc004cos.5 chrM + 1843 4264 1843 1843 1 1843, 4264, uc004cos.5 +uc022bqp.1 chrM - 5543 5566 5543 5543 1 5543, 5566, uc022bqp.1 +uc022bqq.1 chrM - 5585 5606 5585 5585 1 5585, 5606, uc022bqq.1 +uc022bqr.1 chrM - 5690 5714 5690 5690 1 5690, 5714, uc022bqr.1 +uc031tga.1 chrM + 5904 7439 5904 5904 1 5904, 7439, uc031tga.1 +uc022bqs.1 chrM - 7586 15888 7586 7586 2 7586,15503, 7982,15888, uc022bqs.1 +uc011mfi.2 chrM + 7586 9208 7586 7586 1 7586, 9208, uc011mfi.2 +uc022bqt.1 chrM - 8366 14149 8366 8366 2 8366,13449, 8472,14149, uc022bqt.1 +uc022bqu.2 chrM + 10059 10404 10059 10059 1 10059, 10404, uc022bqu.2 +uc004cov.5 chrM + 10470 12138 10470 10470 1 10470, 12138, uc004cov.5 +uc031tgb.1 chrM - 10760 14149 10760 10760 2 10760,13978, 11231,14149, uc031tgb.1 +uc004cow.2 chrM + 12207 12264 12207 12207 1 12207, 12264, uc004cow.2 +uc004cox.4 chrM + 12907 14149 12907 12907 1 12907, 14149, uc004cox.4 +uc022bqv.1 chrM - 14674 14698 14674 14674 1 14674, 14698, uc022bqv.1 +uc022bqw.1 chrM + 14856 15888 14856 14856 1 14856, 15888, uc022bqw.1 +uc022bqx.1 chrM - 15959 16024 15959 15959 1 15959, 16024, uc022bqx.1 +uc004coz.1 chrM + 15998 16571 15998 15998 1 15998, 16571, uc004coz.1