diff README.md @ 6:f201e8c6e004 draft default tip

Uploaded

--- a/README.md	Thu May 22 20:30:19 2014 -0400
+++ b/README.md	Mon Jul 07 19:28:17 2014 -0400
@@ -1,15 +1,15 @@
 galaxy-pcr-markers
 ==================
 
-Scripts for design of PCR-based Marker Assays from DNA sequence variant data, and xml wrappers for use in  Galaxy  workflow environment 
+Scripts for design of PCR-based Marker Assays from DNA sequence variant data, and xml wrappers for use in the  Galaxy  workflow environment 
 
 Also available for download at Galaxy Toolshed http://toolshed.g2.bx.psu.edu/
 hg clone http://toolshed.g2.bx.psu.edu/repos/john-mccallum/pcr_markers
 
-NOTE that the primer design tool *design_primers.py*  relies on EMBOSS ePrimer3 which depends on Primer3 1.1.4.
-The updated tool *design_HRM_primers.py* does not have this restriction and should work with Primer3 V2.
+NOTE that the primer design tool *design_primers.py*  no longer relies on EMBOSS ePrimer3 (which depends on Primer3 1.1.4.)
+ and should work with Primer3 V2.
 
-Please see development branch HRM for optimised design of high-resolution melting PCR assays using the uMelt web service at University of Utah.
+This release adds optimised design of high-resolution melting PCR assays using the uMelt web service provided by the Wittwer Lab at University of Utah https://www.dna.utah.edu/umelt/umelt.html
 
 
 **CITATION**
@@ -19,6 +19,9 @@
 
 BMC Genomics 2012, 13:637  http://www.biomedcentral.com/1471-2164/13/637/abstract
 
+uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application.
+Zachary Dwight1, Robert Palais and Carl T. Wittwer http://bioinformatics.oxfordjournals.org/content/27/7/1019
+
 **Acknowledgements**
 Current development of these tools is funded by the New Zealand Ministry for Business, Innovation & Employment project 'Virtual Institute of Statistical Genetics' (VISG)
 See http://www.visg.co.nz