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+galaxy-pcr-markers
+==================
+
+Scripts for design of PCR-based Marker Assays from DNA sequence variant data, and xml wrappers for use in  Galaxy  workflow environment 
+
+Also available for download at Galaxy Toolshed http://toolshed.g2.bx.psu.edu/
+hg clone http://toolshed.g2.bx.psu.edu/repos/john-mccallum/pcr_markers
+
+NOTE that the primer design tool *design_primers.py*  relies on EMBOSS ePrimer3 which depends on Primer3 1.1.4.
+The updated tool *design_HRM_primers.py* does not have this restriction and should work with Primer3 V2.
+
+Please see development branch HRM for optimised design of high-resolution melting PCR assays using the uMelt web service at University of Utah.
+
+
+**CITATION**
+A Toolkit For Bulk PCR-Based Marker Design From Next-Generation Sequence Data: Application For Development Of A Framework Linkage Map In Bulb Onion (Allium cepa L.) (2012)
+
+Samantha Baldwin, Roopashree Revanna, Susan Thomson, Meeghan Pither-Joyce, Kathryn Wright, Ross Crowhurst, Mark Fiers, Leshi Chen, Richard MacKnight, John A. McCallum
+
+BMC Genomics 2012, 13:637  http://www.biomedcentral.com/1471-2164/13/637/abstract
+
+**Acknowledgements**
+Current development of these tools is funded by the New Zealand Ministry for Business, Innovation & Employment project 'Virtual Institute of Statistical Genetics' (VISG)
+See http://www.visg.co.nz