Trinity assembles transcript sequences from Illumina RNA-Seq data. This tool performs differential expression analyses on a transcriptome assembled with Trinity.
Inputs
This tool uses the matrix produced by 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity' tool.
You must describe your samples and replicates with a tabular file looking like this:
ConditionA | CondA_replicate1 |
ConditionA | CondA_replicate2 |
ConditionB | CondB_replicate1 |
ConditionB | CondB_replicate2 |
ConditionC | CondC_replicate1 |
ConditionC | CondC_replicate2 |
ConditionC | CondC_replicate3 |
This file can be generated with the 'Describe samples and replicates' tool. It will probably be the same file as used in the tool 'RNASeq samples quality check for transcript quantification'. The names in column 2 must match the names given in the tool 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity'.