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Differential expression analysis (version 2.15.1+galaxy0)
output of Build expression matrix - matrix of raw read counts (not normalized!)
file describing samples and replicates
Read edgeR manual to guide your value choice
Additional Options
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Trinity assembles transcript sequences from Illumina RNA-Seq data. This tool performs differential expression analyses on a transcriptome assembled with Trinity.

Inputs

This tool uses the matrix produced by 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity' tool.

You must describe your samples and replicates with a tabular file looking like this:

ConditionA CondA_replicate1
ConditionA CondA_replicate2
ConditionB CondB_replicate1
ConditionB CondB_replicate2
ConditionC CondC_replicate1
ConditionC CondC_replicate2
ConditionC CondC_replicate3

This file can be generated with the 'Describe samples and replicates' tool. It will probably be the same file as used in the tool 'RNASeq samples quality check for transcript quantification'. The names in column 2 must match the names given in the tool 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity'.