Galaxy |

Gene level DE test across two conditions (version 1.0.0)

Gene Expression (tab delimited, please use the unnormalized values, e.g. expected counts from RSEM):

The First Row is Sample Names?:

Enter which condition each sample belongs to (separated by comma, no space please).:

Sample with condition that comes first alphabetically will be in numerator (C1 relative to C2).

Target FDR:

The input Conditions should have exactly two levels. The length of the Condition vector should be exactly the same as the number of columns in the data file (except the gene names column).

Four output files will be generated. Each of the first 3 files contains Posterior probability of being DE (PPDE), Fold Change (RealFC), Posterior Fold Change (PostFC) and normalized gene expressions. The Four files are: Genes with the same order as in input file; Genes sorted by PPDE; DE Genes under target FDR (PPDE>=TargetFDR) and sorted by PPDE; Library size factor for each sample.