Dataset formats
The input datasets are in gd_snp, gd_genotype, and gd_indivs formats. The output dataset is in gd_snp or gd_genotype format. (Dataset missing?)
What it does
For a gd_snp dataset, the user specifies that some of the individuals form a "population", by supplying a list that has been previously created using the Specify Individuals tool. SNPs are then discarded if their total coverage for the population is too low or too high, or if their coverage or quality score for any individual in the population is too low.
The upper and lower bounds on total population coverage can be specified either as read counts or as percentiles (e.g. "5%", with no decimal places). For percentile bounds the SNPs are ranked by read count, so for example, a lower bound of "10%" means that the least-covered 10% of the SNPs will be discarded, while an upper bound of, say, "80%" will discard all SNPs above the 80% mark, i.e. the top 20%. The threshold for the lower bound on individual coverage can only be specified as a plain read count.
For either a gd_snp or gd_genotype dataset, the user can specify a minimum number of defined genotypes (i.e., not -1) and/or a minimum spacing relative to the reference sequence. An error is reported if the user requests a minimum spacing but no reference sequence is available.
Example
input gd_snp:
Contig161_chr1_4641264_4641879 115 C T 73.5 chr1 4641382 C 6 0 2 45 8 0 2 51 15 0 2 72 5 0 2 42 6 0 2 45 10 0 2 57 Y 54 0.323 0 Contig48_chr1_10150253_10151311 11 A G 94.3 chr1 10150264 A 1 0 2 30 1 0 2 30 1 0 2 30 3 0 2 36 1 0 2 30 1 0 2 30 Y 22 +99. 0 Contig20_chr1_21313469_21313570 66 C T 54.0 chr1 21313534 C 4 0 2 39 4 0 2 39 5 0 2 42 4 0 2 39 4 0 2 39 5 0 2 42 N 1 +99. 0 etc.
input individuals:
9 PB1 13 PB2 17 PB3
output when the lower bound on individual coverage is "3":
Contig161_chr1_4641264_4641879 115 C T 73.5 chr1 4641382 C 6 0 2 45 8 0 2 51 15 0 2 72 5 0 2 42 6 0 2 45 10 0 2 57 Y 54 0.323 0 Contig20_chr1_21313469_21313570 66 C T 54.0 chr1 21313534 C 4 0 2 39 4 0 2 39 5 0 2 42 4 0 2 39 4 0 2 39 5 0 2 42 N 1 +99. 0 etc.