| Galaxy wrappers for NCBI BLAST+ suite
=====================================
These wrappers are copyright 2010-2013 by Peter Cock, The James Hutton Institute
(formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved.
See the licence text below.
Currently tested with NCBI BLAST 2.2.26+ (i.e. version 2.2.26 of BLAST+),
and does not work with the NCBI 'legacy' BLAST suite (e.g. blastall).
Note that these wrappers (and the associated datatypes) were originally
distributed as part of the main Galaxy repository, but as of August 2012
moved to the Galaxy Tool Shed as 'ncbi_blast_plus' (and 'blast_datatypes').
My thanks to Dannon Baker from the Galaxy development team for his assistance
with this.
Automated Installation
======================
Galaxy should be able to automatically install the dependencies, i.e. the
'blast_datatypes' repository which defines the BLAST XML file format
('blastxml') and protein and nucleotide BLAST databases ('blastdbp' and
'blastdbn').
You must tell Galaxy about any system level BLAST databases using configuration
files blastdb.loc (nucleotide databases like NT) and blastdb_p.loc (protein
databases like NR), and blastdb_d.loc (protein domain databases like CDD or
SMART) which are located in the tool-data/ folder. Sample files are included
which explain the tab-based format to use.
You can download the NCBI provided databases as tar-balls from here:
ftp://ftp.ncbi.nlm.nih.gov/blast/db/ (nucleotide and protein databases like NR)
ftp://ftp.ncbi.nih.gov/pub/mmdb/cdd/little_endian/ (domain databases like CDD)
Manual Installation
===================
For those not using Galaxy's automated installation from the Tool Shed, put
the XML and Python files in the tools/ncbi_blast_plus/ folder and add the XML
files to your tool_conf.xml as normal (and do the same in tool_conf.xml.sample
in order to run the unit tests). For example, use:
You will also need to install 'blast_datatypes' from the Tool Shed. This
defines the BLAST XML file format ('blastxml') and protein and nucleotide
BLAST databases composite file formats ('blastdbp' and 'blastdbn').
As described above for an automated installation, you must also tell Galaxy
about any system level BLAST databases using the tool-data/blastdb*.loc files.
You must install the NCBI BLAST+ standalone tools somewhere on the system
path. Currently the unit tests are written using "BLAST 2.2.26+".
Run the functional tests (adjusting the section identifier to match your
tool_conf.xml.sample file):
./run_functional_tests.sh -sid NCBI_BLAST+-ncbi_blast_plus_tools
History
=======
v0.0.11 - Final revision as part of the Galaxy main repository, and the
first release via the Tool Shed
v0.0.12 - Implements genetic code option for translation searches.
- Changes to 1000 sequences at a time (to cope with
very large sets of queries where BLAST+ can become memory hungry)
- Include warning that BLAST+ with subject FASTA gives pairwise
e-values
v0.0.13 - Use the new error handling options in Galaxy (the previously
bundled hide_stderr.py script is no longer needed).
v0.0.14 - Support for makeblastdb and blastdbinfo with local BLAST databases
in the history (using work from Edward Kirton), requires v0.0.14
of the 'blast_datatypes' repository from the Tool Shed.
v0.0.15 - Stronger warning in help text against searching against subject
FASTA files (better looking e-values than you might be expecting).
v0.0.16 - Added repository_dependencies.xml for automates installation of the
'blast_datatypes' repository from the Tool Shed.
v0.0.17 - The BLAST+ search tools now default to extended tabular output
(all too often our users where having to re-run searches just to
get one of the missing columns like query or subject length)
v0.0.18 - Defensive quoting of filenames in case of spaces (where possible,
BLAST+ handling of some mult-file arguments is problematic).
v0.0.19 - Added wrappers for rpsblast and rpstblastn, and new blastdb_d.loc
for the domain databases they use (e.g. CDD, PFAM or SMART).
- Correct case of exception regular expression (for error handling
fall-back in case the return code is not set properly).
- Clearer naming of output files.
Developers
==========
This script and related tools are being developed on the 'tools' branch of the
following Mercurial repository:
https://bitbucket.org/peterjc/galaxy-central/
For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball I use
the following command from the Galaxy root folder:
$ ./tools/ncbi_blast_plus/make_ncbi_blast_plus.sh
This simplifies ensuring a consistent set of files is bundled each time,
including all the relevant test files.
Licence (MIT/BSD style)
=======================
Permission to use, copy, modify, and distribute this software and its
documentation with or without modifications and for any purpose and
without fee is hereby granted, provided that any copyright notices
appear in all copies and that both those copyright notices and this
permission notice appear in supporting documentation, and that the
names of the contributors or copyright holders not be used in
advertising or publicity pertaining to distribution of the software
without specific prior permission.
THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL
WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED
WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE
CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT
OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS
OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE
OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE
OR PERFORMANCE OF THIS SOFTWARE.
NOTE: This is the licence for the Galaxy Wrapper only. NCBI BLAST+ and
associated data files are available and licenced separately.
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