| Previous changeset 1:34a799d173f7 (2012-09-07) Next changeset 3:43724ea1c85f (2013-06-27) |
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Commit message:
Add an environment variable for the -M and -T options for memory and thread allocation |
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modified:
cd_hit_est.xml tool_dependencies.xml |
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added:
README |
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| diff -r 34a799d173f7 -r cca0838c1597 README --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README Tue Feb 26 12:11:36 2013 -0600 |
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| @@ -0,0 +1,29 @@ +CD-HIT-EST + +CD-HIT-EST clusters a nucleotide dataset into clusters that meet a user-defined similarity threshold, usually a sequence identity. The input is a DNA/RNA dataset in fasta format and the output are two files: a fasta file of representative sequences and a text file of list of clusters. Since eukaryotic genes usually have long introns, which cause long gaps, it is difficult to make full-length alignments for these genes. So, CD-HIT-EST is good for non-intron containing sequences like EST. + +Ying Huang, Beifang Niu, Ying Gao, Limin Fu and Weizhong Li. CD-HIT Suite: a web server for clustering and comparing biological sequences. Bioinformatics, (2010). 26:680 + + +From: http://weizhong-lab.ucsd.edu/cd-hit/wiki/doku.php?id=cd-hit_user_guide + + +CD-HIT was originally a protein clustering program. The main advantage of this program is its ultra-fast speed. It can be hundreds of times faster than other clustering programs, for example, BLASTCLUST. Therefore it can handle very large databases, like NR. + +The 1st version of this program, CD-HI, was published and released in 2001. The 2nd version, called CD-HIT, was published in 2002 with significant improvements. Since 2004, CD-HIT has been hosted at bioinformatics.org as an open source project. + +Since its release, CD-HIT has been getting more and more popular. It has a significant user base, I estimated at over several thousands users. It is used at many research and educational institutions. For example, at UniProt, CD-HIT is used to generate the UniRef reference data sets (http://www.pir.uniprot.org/database/DBDescription.shtml). It is also used in PDB to treat redundant sequences (http://rutgers.rcsb.org/pdb/redundancy.html). + +In 2006, the 3rd major updates were published and released with abilities to perform various jobs like clustering a protein database, clustering a DNA/RNA database, comparing two databases (protein or DNA/RNA), generating protein families, and many others. + +The CD-HIT web server was implemented in 2009, which allows users to cluster or compare sequences without using command CD-HIT. The server provides interactive interface and additional visualization tools. It also provides pre-calculated and regularly updated sequence clusters for several widely used databases. + +CD-HIT-454, a special version of CD-HIT was implemented in 2010 to cluster artificial duplicated reads in pyrosequencing (454) data. + +Currently, CD-HIT package has many programs: cd-hit, cd-hit-2d, cd-hit-est, cd-hit-est-2d, cd-hit-para, cd-hit-2d-para, psi-cd-hit, psi-cd-hit-2d, cd-hit-454. I also developed some utility tools, written in Perl, to help run and analyze CD-HIT jobs. + + +NOTE to installer: +The tool_dependency will set an environment variable: "CDHIT_SITE_OPTIONS" to -M 4000 -T 0 which will be in the commandline. +You can adjust the values of -M and -T to match the memory and thread capabilities of your site. + |
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| diff -r 34a799d173f7 -r cca0838c1597 cd_hit_est.xml --- a/cd_hit_est.xml Fri Sep 07 13:52:03 2012 -0500 +++ b/cd_hit_est.xml Tue Feb 26 12:11:36 2013 -0600 |
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| @@ -1,10 +1,10 @@ -<tool id="cd_hit_est" name="CD-HIT-EST" version="1.1"> +<tool id="cd_hit_est" name="CD-HIT-EST" version="1.2"> <description>Cluster a nucleotide dataset into representative sequences</description> <requirements> <requirement type="package" version="4.6.1">cd-hit</requirement> </requirements> <command> - cd-hit-est -i $fasta_in -o rep_seq -c $similarity -n $wordsize $strand + cd-hit-est \$CDHIT_SITE_OPTIONS -i "$fasta_in" -o rep_seq -c $similarity -n $wordsize $strand </command> <inputs> <param name="fasta_in" type="data" format="fasta" label="EST Sequences to cluster"/> |
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| diff -r 34a799d173f7 -r cca0838c1597 tool_dependencies.xml --- a/tool_dependencies.xml Fri Sep 07 13:52:03 2012 -0500 +++ b/tool_dependencies.xml Tue Feb 26 12:11:36 2013 -0600 |
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| @@ -7,6 +7,7 @@ <action type="shell_command">make openmp=yes</action> <action type="set_environment"> <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR</environment_variable> + <environment_variable name="CDHIT_SITE_OPTIONS" action="set_to">"-M 4000 -T 0"</environment_variable> </action> </actions> </install> @@ -19,6 +20,9 @@ https://code.google.com/p/cdhit/source/browse/README +Change the CDHIT_SITE_OPTIONS variable in the installed env.sh file to adjust +the maximum memory Mb (-M) or to limit the number of threads (-T) +to match your site </readme> </package> </tool_dependency> |