Repository 'ebseq'
hg clone https://toolshed.g2.bx.psu.edu/repos/ning/ebseq

Changeset 32:d049be55e5cf (2014-06-16)
Previous changeset 31:4b2731768f01 (2014-06-16) Next changeset 33:8a576d034d70 (2014-06-16)
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EBGeneMultiCondTest.R
b
diff -r 4b2731768f01 -r d049be55e5cf EBGeneMultiCondTest.R
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/EBGeneMultiCondTest.R Mon Jun 16 16:22:13 2014 -0400
[
@@ -0,0 +1,68 @@
+sink(file="/tmp/none")
+sink("/dev/null")
+options(warn=-1)
+options(echo=F) 
+
+invisible("EBSeq")
+suppressMessages(library("EBSeq"))
+
+args <- commandArgs(trailingOnly = T)
+inputfile <- args[1]
+WhetherSampleName <- args[2]
+CondIn <- args[3]
+PatternFile <- args[4]
+outputfile <- args[5]
+MAP.out<-args[6]
+Sizesout <-args[7]
+
+#write.table(PatternFile,outputfile,quote=F,col.names=T,row.names=T,sep = "\t")
+
+#write.table(args,outputfile,quote=F,col.names=T,row.names=T,sep = "\t")
+
+Conditions=strsplit(CondIn,split=",")[[1]]
+
+if(WhetherSampleName=="y"){
+ ReadIn=read.table(inputfile,stringsAsFactors=F,header=T, sep="\t")
+ Names=names(ReadIn)[-1]
+ }
+if(WhetherSampleName=="n"){
+ ReadIn=read.table(inputfile,stringsAsFactors=F,header=F, sep="\t")
+ Names=paste0("S",1:length(Conditions))
+}
+
+#PatternIn=read.table(PatternFile,stringsAsFactors=F,header=T,sep="\t")
+PatternIn=read.table(PatternFile,stringsAsFactors=F,header=T,sep="\t",row.names=1) ##cms - changed on 11/15/13
+
+if(class(ReadIn[[1]])=="character"){
+ GeneMat=do.call(cbind,ReadIn[-1])
+ rownames(GeneMat)=ReadIn[[1]]
+ colnames(GeneMat)=Names
+}
+if(class(ReadIn[[1]])=="numeric"){
+ GeneMat=data.matrix(ReadIn)
+ colnames(GeneMat)=Names
+ }
+
+Patterns=data.matrix(PatternIn)
+
+
+Sizes=MedianNorm(GeneMat)
+EBOut=EBMultiTest(Data=GeneMat,Conditions=as.factor(Conditions),
+ AllParti=Patterns,sizeFactors=Sizes, maxround=5)
+PPout=GetMultiPP(EBOut)
+MultiPP=PPout$PP
+MultiMAP=PPout$MAP
+Data.norm=round(GetNormalizedMat(GeneMat, Sizes),2)
+
+Mat=cbind(MultiMAP,Data.norm[names(MultiMAP),])
+
+colnames(Mat)=
+c("MAP",Names)
+options(warn=-1)
+
+#write.table(round(MultiPP,2),file=outputfile,quote=F,col.names=T,row.names=T,sep = "\t")
+write.table(round(MultiPP,2),file=outputfile,quote=F,col.names=NA,row.names=T,sep = "\t")  ##cms - changed on 11/15/13
+#write.table(Mat,file=MAP.out ,quote=F,col.names=T,row.names=T,sep = "\t")
+write.table(Mat,file=MAP.out ,quote=F,col.names=NA,row.names=T,sep = "\t")  ##cms - changed on 11/15/13
+write.table(Sizes,file=Sizesout,quote=F,col.names=F,row.names=F,sep = "\t")
+