annotate [APliBio]Nebula tools suite/Nebula/Peak2Bed/peak2bed.pl @ 3:1c699789d6d3 draft

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author alermine
date Wed, 14 Nov 2012 06:02:48 -0500
parents 2ec3ba0e9e70
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7 #::::::::::3@@@@@@@@@@@@@@@@@@@@@BEEESSE5EEEEBBM@@@@@@@@@@@@@@@@@@@@@@@@@@
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8 #::::::::::3@@@@@@@@@@@@@@@@@@@@BEEEEEE35EE55E2355E5SBMB@@@@@@@@@@@@@@@@@$
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9 #::::::::::@@@@@@@@@@@@@@@@@@@EEEE55533t3tttt::::::!!!!7755E755SBBMMM@@@MM
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17 #::::::::::3B@@@@@@@@@@@@@@@@@@EEEEEEE3tt:czzztti;:::::::::::::::::::::::3
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18 #::::ttt::::3@@@@@@@@@@@@@@@@EEEEE5EE25Ezt1EEEz5Etzzz;;;;:::::::::::::::::
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39 #::::::::::::::::::::::::::::::::75B@@@@@EEEtt;:::::::::;zz@@@@BEEEtz:::::
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44 #::::::::::::::::::::::::::::;@@@@@@@@@@@@@@@@@@@@@@@@@@E@@EEEEEEEEEEEE@@@
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45 #::::::::::::::::::::::::::g@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@EEEE3EEEE@@@@@@@
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47 #:::::::::::::::::;g@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@E@@@@@@EEEtg@@@@@@@@@@@@
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48 #::::::::::::::;@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@EEEE@@@@@@@@@@@@@@@@@@@@@@@@
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49 #:::::::::::::@@@@@@@@@@@@@@@@@$@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
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50 #::::::::::;@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
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51 #
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52 # Copyleft ↄ⃝ 2012 Institut Curie
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53 # Author(s): Valentina Boeva, Alban Lermine (Institut Curie) 2012
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54 # Contact: valentina.boeva@curie.fr, alban.lermine@curie.fr
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55 # This software is distributed under the terms of the GNU General
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56 # Public License, either Version 2, June 1991 or Version 3, June 2007.
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57
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58 #!/usr/bin/perl
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59
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60 use strict;
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61 use warnings;
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62 use diagnostics;
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63
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64 my $usage = qq{
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65 $0
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66
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67 -----------------------------
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68 mandatory parameters:
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69
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70 -f peaks
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71 -t min peak height
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72 -o output file
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73
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74 -----------------------------
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75 optional parameters:
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76 -n name
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77 none
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78 };
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79
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80 if(scalar(@ARGV) == 0){
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81 print $usage;
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82 exit(0);
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83 }
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84
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85 ## mandatory arguments
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86
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87 my $filename = "";
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88 my $output_fname = "";
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89
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90 my $minPeakH = 0;
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91
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92 my $chromLengthsFile="";
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93 my $expName = "User Track";
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94
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95 ## optional arguments
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96
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97 ## parse command line arguments
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98
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99 while(scalar(@ARGV) > 0){
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100 my $this_arg = shift @ARGV;
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101 if ( $this_arg eq '-h') {print "$usage\n"; exit; }
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102
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103 elsif ( $this_arg eq '-f') {$filename = shift @ARGV;}
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104 elsif ( $this_arg eq '-t') {$minPeakH = shift @ARGV;}
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105 elsif ( $this_arg eq '-o') {$output_fname = shift @ARGV;}
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106 elsif ( $this_arg eq '-g') {$chromLengthsFile = shift @ARGV;}
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107 elsif ( $this_arg eq '-n') {$expName = shift @ARGV;}
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108 elsif ( $this_arg =~ m/^-/ ) { print "unknown flag: $this_arg\n";}
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109 }
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110
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111 if ( $filename eq ""){
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112 die "you should specify chip file\n";
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113 }
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114 if( $output_fname eq ""){
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115 die "you should specify output filename\n";
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116 }
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117
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118 $minPeakH-=0.5 unless ($minPeakH=~m/0\.5/);
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119
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120 #read chromosome lengths if provided:
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121 my %max;
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122 if ($chromLengthsFile ne "") {
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123 open FILE, "< $chromLengthsFile " || die "$chromLengthsFile : $!\n";
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124 while(<FILE>){
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125 chomp;
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126 if (/(chr\S+)\s(\d+)/) {
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127 $max{$1}=$2;
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128 }
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129 }
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130 close FILE;
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131 }
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132
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133 ######
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134
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135 print "\n-----------------\n\n";
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136
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137 my %hash;
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138 my $chipCount = 0;
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139 my @header;
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140
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141
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142 open FILE, "< $filename " || die "$filename : $!\n";
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143 open OUT, "> $output_fname" || die "$output_fname: $!\n";
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144 print OUT "track name=\'$expName\' description=\'$expName\'\n";
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145 my $count = 0;
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146 my $scount = 0;
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147
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148 while(<FILE>){
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149 chomp;
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150 next if (/max/);
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151 next if (/track/);
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152 next if (/^\#/);
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153 my @fields = split(/\t/,$_);
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154 my $entry = $fields[1]."\t".$fields[2]."\t".$fields[3];
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155 $count++;
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156 if ($fields[5]>=$minPeakH) {
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157 $scount ++;
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158 $fields[1]= "chr".$fields[1] unless ($fields[1]=~m/chr/);
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159
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160 if ($chromLengthsFile ne "") {my $maxV = $max{$fields[1]}; $fields[2]= min($fields[2],$maxV);$fields[3]=min($fields[3],$maxV);$fields[4]=min($fields[4],$maxV); }
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161 print OUT join("\t",$fields[1],$fields[2],$fields[3],$fields[4],$fields[5],"+",$fields[2],$fields[3],"255,120,11","1",$fields[3]-$fields[2],0,"\n");
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162 }
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163 }
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164
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165 close FILE;
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166 close OUT;
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167 print "read: $count peaks; selected: $scount\n";
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168
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169 sub min {
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170 my ($a,$b) = @_;
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171 if($a<$b){
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172 return $a;
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173 }
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174 $b;
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175 }
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176