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1 <tool id="vcfrandomsample" name="VCFrandomSample:" version="0.0.1">
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2 <requirements>
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3 <requirement type="package" version="586c5ae5d57a38dae6b32ea831fb1f7cfa14c9bd">vcflib</requirement>
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4 </requirements>
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5 <description>Randomly sample sites from VCF dataset</description>
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6 <command>
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7
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8 #if $adv_options.adv_options_selector == True:
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9 vcfrandomsample -s "${adv_options.scale_by}" -p ${random_seed} -r ${rate} "${vcf_input}" > "${out_file1}"
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10 #else:
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11 vcfrandomsample -p ${random_seed} -r ${rate} "${vcf_input}" > "${out_file1}"
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12 #end if
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13 </command>
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14 <inputs>
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15 <param format="vcf" name="vcf_input" type="data" label="Select random sites from"/>
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16 <param name="rate" type="float" value="0.1" label="base sampling probability per locus" help="-r, --rate"/>
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17 <param name="random_seed" type="integer" value="17823618326" label="use this random seed" help="-p, --random-seed; use this to make results reproducible" />
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18 <conditional name="adv_options">
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19 <param name="adv_options_selector" type="boolean" truevalue="use_adv_controls" label="Advanced controls" help="Allows you to specify options that are not listed above"/>
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20 <when value="use_adv_controls">
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21 <param name="scale_by" type="text" value="AF" label="scale sampling likelihood by this INFO field" help="-s, --scale-by; this only works for Float info fields such as AF" />
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22 </when>
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23 </conditional>
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24 </inputs>
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25 <outputs>
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26 <data format="vcf" name="out_file1" />
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27 </outputs>
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28 <tests>
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29 <test>
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30 <param name="vcf_input" value="vcflib.vcf"/>
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31 <param name="rate" value="0.2" />
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32 <param name="random_seed" value="1" />
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33 <param name="adv_options_selector" value="False" />
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34 <output name="out_file1" file="vcfrandomsample-test1.vcf" lines_diff="2" />
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35 </test>
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36 </tests>
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37 <help>
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38
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39 Randomly sample sites from an input VCF file. Scale the sampling probability by the field specified by --scale-by (see advanced controls). This may be
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40 used to provide uniform sampling across allele frequencies, for instance (AF field in this case).
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41
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42 Options::
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43
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44 -r, --rate RATE base sampling probability per locus
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45 -s, --scale-by KEY scale sampling likelihood by this Float info field
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46 -p, --random-seed N use this random seed
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47
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48 ----
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49
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50 Vcfrandomsample is a part of VCFlib toolkit developed by Erik Garrison (https://github.com/ekg/vcflib).
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51
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52 </help>
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53 </tool>
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