# HG changeset patch # User artbio # Date 1641730538 0 # Node ID 6815060fb0561f57314371c212a105000041caa9 # Parent ba3e6a71acb80efdc13fe0206d4f1f514d72b0a6 "planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 667afd5a5408321a31cdebab75b378e694deab07" diff -r ba3e6a71acb8 -r 6815060fb056 artbio_bam_cleaning.xml --- a/artbio_bam_cleaning.xml Fri Dec 31 00:04:07 2021 +0000 +++ b/artbio_bam_cleaning.xml Sun Jan 09 12:15:38 2022 +0000 @@ -1,4 +1,4 @@ - + on flags and PCR Duplicates and MD recalibration @@ -26,9 +26,9 @@ > $calmd #else if $specify_outputs == 'both': | tee $calmd - | sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality <= 254' -f 'bam' /dev/stdin > $fullfilter + | sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality <= 254 and proper_pair' -f 'bam' /dev/stdin > $fullfilter #else: - | sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality <= 254' -f 'bam' /dev/stdin > $fullfilter + | sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality <= 254 and proper_pair' -f 'bam' /dev/stdin > $fullfilter #end if ]]> @@ -40,7 +40,7 @@ aberrant Mapping Quality alignements generated by calMD recalibration. One, the other, or both types of outputs can be retained by the tool"> - + diff -r ba3e6a71acb8 -r 6815060fb056 test-data/full.bam Binary file test-data/full.bam has changed