# HG changeset patch # User artbio # Date 1613434797 0 # Node ID a1462dd52f1753a9bb2edc99e6bb0b5887f21040 # Parent c973ff00c785447e45814cab8747ffc3e63da492 "planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit b022da54be013bf593ad96de6e7bd8b9e6bb5523" diff -r c973ff00c785 -r a1462dd52f17 artbio_bam_cleaning.xml --- a/artbio_bam_cleaning.xml Sun Feb 14 22:45:31 2021 +0000 +++ b/artbio_bam_cleaning.xml Tue Feb 16 00:19:57 2021 +0000 @@ -1,4 +1,4 @@ - + on flags and PCR Duplicates and MD recalibration @@ -23,41 +23,24 @@ | samtools rmdup - - |tee $input_base".filt1.dedup.bam"| bamleftalign --fasta-reference reference.fa -c --max-iterations "5" - | samtools calmd -C 50 -b -@ \${GALAXY_SLOTS:-2} - reference.fa > $calmd - #if $pipeline == 'fullfilter': && sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality <= 254' -f 'bam' -o $fullfilter $calmd - #end if ]]> - - - - - - pipeline == 'CalMD' - - - pipeline == 'fullfilter' - - + + + - - - - - - - ARTbio bam cleaning overview