# HG changeset patch # User artbio # Date 1555084895 14400 # Node ID 8b1020c25f0f5b3459709cef31d5e33914721788 # Parent 563337e780ce3e742bc2154e66966ba7b59b0322 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/cpm_tpm_rpk commit 9149001c65de633ddfd2f91cf208074e40482ce3 diff -r 563337e780ce -r 8b1020c25f0f cpm_tpm_rpk.R --- a/cpm_tpm_rpk.R Wed Feb 06 19:31:57 2019 -0500 +++ b/cpm_tpm_rpk.R Fri Apr 12 12:01:35 2019 -0400 @@ -29,7 +29,7 @@ c("-t", "--type"), default = 'cpm', type = 'character', - help = "Transformation type, either cpm, tpm or rpk [default : '%default' ]" + help = "Transformation type, either cpm, tpm, rpk or none[default : '%default' ]" ), make_option( c("-s", "--sep"), @@ -127,7 +127,7 @@ } else if ((opt$type == "tpm" | opt$type == "rpk") & opt$gene == "") { stop("At least two arguments must be supplied (count data and gene length file).\n", call. = FALSE) -} else if (opt$type != "tpm" & opt$type != "rpk" & opt$type != "cpm") { +} else if (opt$type != "tpm" & opt$type != "rpk" & opt$type != "cpm" & opt$type != "none") { stop("Wrong transformation requested (--type option) must be : cpm, tpm or rpk.\n", call. = FALSE) } @@ -153,6 +153,7 @@ data = read.table( opt$data, + check.names = FALSE, header = opt$colnames, row.names = 1, sep = opt$sep @@ -177,6 +178,8 @@ res = as.data.frame(apply(data, 2, tpm, length = gene_length), row.names = rownames(data)) if (opt$type == "rpk") res = as.data.frame(apply(data, 2, rpk, length = gene_length), row.names = rownames(data)) +if (opt$type == "none") + res = data colnames(res) = colnames(data) @@ -206,7 +209,7 @@ embedding$Class <- as.factor(sub("Class_", "", rownames(tdf))) gg_legend = theme(legend.position="none") ggplot(embedding, aes(x=V1, y=V2)) + - geom_point(size=1.25, color='red') + + geom_point(size=1, color='red') + gg_legend + xlab("") + ylab("") + @@ -215,16 +218,20 @@ geom_text(aes(label=Class),hjust=-0.2, vjust=-0.5, size=2.5, color='darkblue') } ggsave(file=opt$tsne_out, device="pdf") - # make PCA and plot result with ggfortify + # make PCA and plot result with ggfortify (autoplot) tdf.pca <- prcomp(tdf, center = TRUE, scale. = T) if (opt$tsne_labels == TRUE) { - autoplot(tdf.pca, shape=F, label=T, label.size=2.5, colour="darkred") + + autoplot(tdf.pca, shape=F, label=T, label.size=2.5, label.vjust=1.2, + label.hjust=1.2, + colour="darkblue") + + geom_point(size=1, color='red') + xlab(paste("PC1",summary(tdf.pca)$importance[2,1]*100, "%")) + ylab(paste("PC2",summary(tdf.pca)$importance[2,2]*100, "%")) + ggtitle('PCA') ggsave(file=opt$pca_out, device="pdf") } else { - autoplot(tdf.pca, shape=T, colour="red") + + autoplot(tdf.pca, shape=T, colour="darkblue") + + geom_point(size=1, color='red') + xlab(paste("PC1",summary(tdf.pca)$importance[2,1]*100, "%")) + ylab(paste("PC2",summary(tdf.pca)$importance[2,2]*100, "%")) + ggtitle('PCA') diff -r 563337e780ce -r 8b1020c25f0f cpm_tpm_rpk.xml --- a/cpm_tpm_rpk.xml Wed Feb 06 19:31:57 2019 -0500 +++ b/cpm_tpm_rpk.xml Fri Apr 12 12:01:35 2019 -0400 @@ -1,4 +1,4 @@ - + from raw counts expression values r-optparse @@ -64,6 +64,7 @@ + @@ -97,6 +98,8 @@ + + @@ -108,10 +111,10 @@ - - - - + + + + @@ -136,6 +139,19 @@ + + + + + + + + + + + + + diff -r 563337e780ce -r 8b1020c25f0f test-data/none.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/none.tab Fri Apr 12 12:01:35 2019 -0400 @@ -0,0 +1,216 @@ +Features 20nM_1 20sM_4 32nM_7 32sM_10 40nM_13 40sM_16 20n1d_a 20n1d_b 30n5h_c1 30n5h_c2 30s5h_c3 30s5h_c4 40n5h_c5 40n5h_c6 40s5h_c7 40s5h_c8 20n1d_c 20s1d_d 20s1d_e 20s1d_f 30n1d_g 30n1d_h 30n1d_i 30s1d_j 30s1d_k 30s1d_l 40n1d_m 40n1d_n 40n1d_o 40s1d_p 40s1d_q 40s1d_r +5S_DM 0 0 0 0 2.52271103749722 0 0 1.46876851779219 1.10607886502204 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.51278181441303 2.52260597944523 0 0 0 2.05794942496048 0.646139123391797 +ACCORD2_I-int 13.4694287027486 19.7873189245625 17.5674602110498 50.6988197972739 17.6589772624805 19.313391508794 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