view fetch_fasta_from_NCBI.py @ 5:706fe8139955 draft

"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/fetch_fasta_from_ncbi commit b5ef783237b244d684e26b1ed1cc333a8305ce3e"

#!/usr/bin/env python
# -*- coding: utf-8 -*-
"""
From a taxonomy ID retrieves all the nucleotide sequences
It returns a multiFASTA nuc/prot file

Entrez Database  UID common name  E-utility Database Name
Nucleotide       GI number        nuccore
Protein          GI number        protein

Retrieve strategy:

esearch to get total number of UIDs (count)
esearch to get UIDs in batches
loop untile end of UIDs list:
  epost to put a batch of UIDs in the history server
  efetch to retrieve info from previous post

retmax of efetch is 1/10 of declared value from NCBI

queries are 1 sec delayed, to satisfy NCBI guidelines
(more than what they request)
"""
import argparse
import http.client
import logging
import re
import sys
import time
from urllib import error, parse, request


LOG_FORMAT = '%(asctime)s|%(levelname)-8s|%(message)s'
LOG_DATEFMT = '%Y-%m-%d %H:%M:%S'
LOG_LEVELS = ['DEBUG', 'INFO', 'WARNING', 'ERROR', 'CRITICAL']


class QueryException(Exception):
    pass


class Eutils:

    def __init__(self, options, logger):
        """
        Initialize retrieval parameters
        """
        self.logger = logger
        self.base = "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/"
        self.query_string = options.query_string
        self.dbname = options.dbname
        if options.get_fasta:
            self.get_fasta = options.get_fasta
        self.ids = []
        self.retmax_esearch = 100000
        self.retmax_efetch = 500
        self.webenv = ''
        self.usehistory = ''
        self.query_key = ''
        self.iuds_file = options.iuds_file
        if self.iuds_file:
            with open(self.iuds_file, 'r') as f:
                for line in f:
                    self.ids.append(line.rstrip())
        self.count = len(self.ids)  # 0 if query, some value if iuds_file

    def retrieve(self):
        """
        Retrieve the iuds and fastas corresponding to the query
        """
        if len(self.ids) == 0:  # retrieving from query (not required for file)
            self.count = self.ecount()
        # If no UIDs were found from query or file, exit
        if self.count == 0:
            self.logger.info("found no UIDs. Exiting script.")
            sys.exit(-1)
        if not self.iuds_file:
            self.get_uids_list()
            self.print_uids_list()
        else:
            # as self.ids already implemented
            self.print_uids_list()
        if self.get_fasta:
            try:
                self.get_sequences()
            except QueryException as e:
                self.logger.error("Exiting script.")
                raise e

    def print_uids_list(self):
        with open("retrieved_uid_list.txt", 'w') as f:
            f.write('\n'.join(self.ids))

    def ecount(self):
        """
        just to retrieve Count (number of UIDs)
        Total number of UIDs from the retrieved set to be shown in the XML
        output (default=20). By default, ESearch only includes the first 20
        UIDs retrieved in the XML output. If usehistory is set to 'y',
        the remainder of the retrieved set will be stored on the History server
        http://www.ncbi.nlm.nih.gov/books/NBK25499/#chapter4.EFetch
        """
        querylog = self.esearch(self.dbname, self.query_string, '', '',
                                'count')
        self.logger.debug("Query response:")
        for line in querylog:
            line = line.decode('utf-8')
            self.logger.debug(line.rstrip())
            if '</Count>' in line:
                count = int(line.split("<Count>")[1].split("</Count>")[0])
        self.logger.info("Found %d UIDs" % count)
        return count

    def get_uids_list(self):
        """
        Increasing retmax allows more of the retrieved UIDs to be included in
        the XML output, up to a maximum of 100,000 records.
        from http://www.ncbi.nlm.nih.gov/books/NBK25499/#chapter4.ESearch
        """
        retmax = self.retmax_esearch
        self.logger.info("retmax = %s, self.count = %s" % (retmax, self.count))
        if (int(self.count) > retmax):
            num_batches = int(self.count / retmax) + 1
            self.usehistory = 'y'
            self.logger.info("Batch size for esearch action: %d UIDs" % retmax)
            self.logger.info("Number of batches for esearch action: %s"
                             % num_batches)
            querylog = self.esearch(self.dbname, self.query_string, '', '', '')
            for line in querylog:
                line = line.decode('utf-8')
                self.logger.debug(line.rstrip())
                if '<WebEnv>' in line:
                    self.webenv = line.split("<WebEnv>")[1].split(
                                             "</WebEnv>")[0]
            self.logger.info("Will use webenv %s" % self.webenv)
            for n in range(num_batches):
                querylog = self.esearch(self.dbname, self.query_string,
                                        n*retmax, retmax, '')
                for line in querylog:
                    line = line.decode('utf-8')
                    if '<Id>' in line and '</Id>' in line:
                        uid = line.split("<Id>")[1].split("</Id>")[0]
                        self.ids.append(uid)
                self.logger.info("Retrieved %d UIDs" % len(self.ids))

        else:
            self.logger.info("Batch size for esearch action: %d UIDs" % retmax)
            self.logger.info("Number of batches for esearch action: 1")
            querylog = self.esearch(self.dbname, self.query_string, 0,
                                    retmax, '')
            for line in querylog:
                line = line.decode('utf-8')
                if '<Id>' in line and '</Id>' in line:
                    uid = line.split("<Id>")[1].split("</Id>")[0]
                    self.ids.append(uid)
            self.logger.info("Retrieved %d UIDs" % len(self.ids))
        return self.ids

    def get_sequences(self):
        batch_size = self.retmax_efetch
        count = self.count
        uids_list = self.ids
        self.logger.info("Batch size for efetch action: %d" % batch_size)
        self.logger.info("Number of batches for efetch action: %d" %
                         ((count / batch_size) + 1))
        with open(self.get_fasta, 'w') as out:
            for start in range(0, count, batch_size):
                end = min(count, start+batch_size)
                batch = uids_list[start:end]
                self.logger.info("retrieving batch %d" %
                                 ((start / batch_size) + 1))
                try:
                    mfasta = self.efetch(self.dbname, ','.join(batch))
                    out.write(mfasta + '\n')
                except QueryException as e:
                    self.logger.error("%s" % e.message)
                    raise e
        request.urlcleanup()

    def efetch(self, db, uid_list):
        url = self.base + "efetch.fcgi"
        self.logger.debug("url_efetch: %s" % url)
        values = {'db': db,
                  'id': uid_list,
                  'rettype': "fasta",
                  'retmode': "text",
                  'usehistory': self.usehistory,
                  'WebEnv': self.webenv}
        data = parse.urlencode(values)
        req = request.Request(url, data.encode('utf-8'))
        self.logger.debug("data: %s" % str(data))
        serverTransaction = False
        counter = 0
        response_code = 0
        while not serverTransaction:
            counter += 1
            self.logger.info("Server Transaction Trial:  %s" % (counter))
            try:
                self.logger.debug("Going to open")
                response = request.urlopen(req)
                self.logger.debug("Going to get code")
                response_code = response.getcode()
                self.logger.debug("Going to read, de code was : %s",
                                  str(response_code))
                fasta = response.read()
                self.logger.debug("Did all that")
                response.close()
                if((response_code != 200) or
                   (b"Resource temporarily unavailable" in fasta) or
                   (b"Error" in fasta) or (not fasta.startswith(b">"))):
                    serverTransaction = False
                    if (response_code != 200):
                        self.logger.info("urlopen error: Response code is not\
                                         200")
                    elif ("Resource temporarily unavailable" in fasta):
                        self.logger.info("Ressource temporarily unavailable")
                    elif ("Error" in fasta):
                        self.logger.info("Error in fasta")
                    else:
                        self.logger.info("Fasta doesn't start with '>'")
                else:
                    serverTransaction = True
            except error.HTTPError as e:
                serverTransaction = False
                self.logger.info("urlopen error:%s, %s" % (e.code, e.read()))
            except error.URLError as e:
                serverTransaction = False
                self.logger.info("urlopen error: Failed to reach a server")
                self.logger.info("Reason :%s" % (e.reason))
            except http.client.IncompleteRead as e:
                serverTransaction = False
                self.logger.info("IncompleteRead error:  %s" % (e.partial))
            if (counter > 500):
                serverTransaction = True
        if (counter > 500):
            raise QueryException({"message":
                                  "500 Server Transaction Trials attempted for\
                                  this batch. Aborting."})
        fasta = self.sanitiser(self.dbname, fasta.decode('utf-8'))
        time.sleep(0.1)
        return fasta

    def esearch(self, db, term, retstart, retmax, rettype):
        url = self.base + "esearch.fcgi"
        self.logger.debug("url: %s" % url)
        values = {'db': db,
                  'term': term,
                  'rettype': rettype,
                  'retstart': retstart,
                  'retmax': retmax,
                  'usehistory': self.usehistory,
                  'WebEnv': self.webenv}
        data = parse.urlencode(values)
        self.logger.debug("data: %s" % str(data))
        req = request.Request(url, data.encode('utf-8'))
        response = request.urlopen(req)
        querylog = response.readlines()
        response.close()
        time.sleep(1)
        return querylog

    def sanitiser(self, db, fastaseq):
        if(db not in "nuccore protein"):
            return fastaseq
        regex = re.compile(r"[ACDEFGHIKLMNPQRSTVWYBZ]{49,}")
        sane_seqlist = []
        seqlist = fastaseq.split('\n\n')
        for seq in seqlist[:-1]:
            fastalines = seq.split("\n")
            if len(fastalines) < 2:
                self.logger.info("Empty sequence for %s" %
                                 ("|".join(fastalines[0].split("|")[:4])))
                self.logger.info("%s download is skipped" %
                                 ("|".join(fastalines[0].split("|")[:4])))
                continue
            if db == "nuccore":
                badnuc = 0
                for nucleotide in fastalines[1]:
                    if nucleotide not in "ATGC":
                        badnuc += 1
                if float(badnuc)/len(fastalines[1]) > 0.4:
                    self.logger.info("%s ambiguous nucleotides in %s\
                                     or download interrupted at this offset\
                                     | %s" % (float(badnuc)/len(fastalines[1]),
                                              "|".join(fastalines[0].split("|")
                                                       [:4]),
                                              fastalines[1]))
                    self.logger.info("%s download is skipped" %
                                     fastalines[0].split("|")[:4])
                    continue
                """ remove spaces and trim the header to 100 chars """
                fastalines[0] = fastalines[0].replace(" ", "_")[:100]
                cleanseq = "\n".join(fastalines)
                sane_seqlist.append(cleanseq)
            elif db == "protein":
                fastalines[0] = fastalines[0][0:100]
                fastalines[0] = fastalines[0].replace(" ", "_")
                fastalines[0] = fastalines[0].replace("[", "_")
                fastalines[0] = fastalines[0].replace("]", "_")
                fastalines[0] = fastalines[0].replace("=", "_")
                """ because blast makedb doesn't like it """
                fastalines[0] = fastalines[0].rstrip("_")
                fastalines[0] = re.sub(regex, "_", fastalines[0])
                cleanseq = "\n".join(fastalines)
                sane_seqlist.append(cleanseq)
        self.logger.info("clean sequences appended: %d" % (len(sane_seqlist)))
        return "\n".join(sane_seqlist)


def command_parse():
    parser = argparse.ArgumentParser(description='Retrieve data from NCBI')
    parser.add_argument('--query', '-i', dest='query_string',
                        default=None, help='NCBI Query String')
    parser.add_argument('--iud_file', dest='iuds_file', default=None,
                        help='input list of iuds to be fetched')
    parser.add_argument('--dbname', '-d', dest='dbname', help='database type')
    parser.add_argument('--fasta', '-F', dest='get_fasta', default=False,
                        help='file with retrieved fasta sequences')
    parser.add_argument('--logfile', '-l',  help='log file (default=stderr)')
    parser.add_argument('--loglevel', choices=LOG_LEVELS, default='INFO',
                        help='logging level (default: INFO)')
    args = parser.parse_args()

    if args.query_string is not None and args.iuds_file is not None:
        parser.error('Please choose either fetching by query (--query) \
                      or by uid list (--iud_file)')
    return args


def __main__():
    """ main function """
    args = command_parse()
    log_level = getattr(logging, args.loglevel)
    kwargs = {'format': LOG_FORMAT,
              'datefmt': LOG_DATEFMT,
              'level': log_level}
    if args.logfile:
        kwargs['filename'] = args.logfile
    logging.basicConfig(**kwargs)
    logger = logging.getLogger('data_from_NCBI')
    E = Eutils(args, logger)
    try:
        E.retrieve()
    except Exception:
        sys.exit(-1)


if __name__ == "__main__":
    __main__()