Mercurial > repos > artbio > gsc_center_scale
comparison center_scale.R @ 2:163befe5f05b draft default tip
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/main/tools/gsc_center_scale commit 41ba5435a8ad12c1fe0703a9ce44b759003f3f73
author | artbio |
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date | Sun, 15 Oct 2023 16:16:16 +0000 |
parents | a96cc346819c |
children |
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1:a96cc346819c | 2:163befe5f05b |
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1 options( show.error.messages=F, | 1 options(show.error.messages = FALSE, |
2 error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) | 2 error = function() { |
3 cat(geterrmessage(), file = stderr()) | |
4 q("no", 1, FALSE) | |
5 } | |
6 ) | |
3 loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") | 7 loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") |
4 warnings() | 8 warnings() |
5 library(optparse) | 9 library(optparse) |
6 | 10 |
7 # Arguments | 11 # Arguments |
8 option_list = list( | 12 option_list <- list( |
9 make_option( | 13 make_option( |
10 '--data', | 14 "--data", |
11 default = NA, | 15 default = NA, |
12 type = 'character', | 16 type = "character", |
13 help = "Input file that contains values to transform. Must be tabular separated, | 17 help = "Input file that contains values to transform. Must be tabular separated, |
14 with columns and row names, variables in rows, observations in columns [default : '%default' ]" | 18 with columns and row names, variables in rows, observations in columns [default : '%default' ]" |
15 ), | 19 ), |
16 make_option( | 20 make_option( |
17 '--center', | 21 "--center", |
18 default = TRUE, | 22 default = TRUE, |
19 type = 'logical', | 23 type = "logical", |
20 help = "center data to the mean [default : '%default' ]" | 24 help = "center data to the mean [default : '%default' ]" |
21 ), | 25 ), |
22 make_option( | 26 make_option( |
23 '--scale', | 27 "--scale", |
24 default = TRUE, | 28 default = TRUE, |
25 type = 'logical', | 29 type = "logical", |
26 help = "scale data to standard deviation [default : '%default' ]" | 30 help = "scale data to standard deviation [default : '%default' ]" |
27 ), | 31 ), |
28 make_option( | 32 make_option( |
29 '--factor', | 33 "--factor", |
30 default = '', | 34 default = "", |
31 type = 'character', | 35 type = "character", |
32 help = "A two-column observations|factor_levels table, to group observations to be transformed by levels [default : '%default' ]" | 36 help = "A two-column observations|factor_levels table, to group observations to be transformed by levels [default : '%default' ]" |
33 ), | 37 ), |
34 make_option( | 38 make_option( |
35 '--output', | 39 "--output", |
36 default = 'res.tab', | 40 default = "res.tab", |
37 type = 'character', | 41 type = "character", |
38 help = "Table of transformed values [default : '%default' ]" | 42 help = "Table of transformed values [default : '%default' ]" |
39 ) | 43 ) |
40 ) | 44 ) |
41 | 45 |
42 transform <- function(df, center=TRUE, scale=TRUE) { | 46 transform <- function(df, center = TRUE, scale = TRUE) { |
43 transfo <- scale( | 47 transfo <- scale(t(df), |
44 t(df), | 48 center = center, |
45 center=center, | 49 scale = scale |
46 scale=scale | 50 ) |
47 ) | 51 return(as.data.frame(t(transfo))) |
48 return(as.data.frame(t(transfo))) | |
49 } | 52 } |
50 | 53 |
51 opt = parse_args(OptionParser(option_list = option_list), | 54 opt <- parse_args(OptionParser(option_list = option_list), |
52 args = commandArgs(trailingOnly = TRUE)) | 55 args = commandArgs(trailingOnly = TRUE)) |
53 | 56 |
54 data = read.table( | 57 data <- read.delim( |
55 opt$data, | 58 opt$data, |
59 check.names = FALSE, | |
60 header = TRUE, | |
61 row.names = 1, | |
62 sep = "\t" | |
63 ) | |
64 | |
65 if (opt$factor != "") { | |
66 data_factor <- read.delim( | |
67 opt$factor, | |
56 check.names = FALSE, | 68 check.names = FALSE, |
57 header = TRUE, | 69 header = TRUE, |
58 row.names = 1, | 70 sep = "\t", |
59 sep = '\t' | 71 stringsAsFactors = TRUE |
60 ) | 72 ) |
61 | 73 colnames(data_factor) <- c("cellid", "level") |
62 if (opt$factor != '') { | 74 data_transformed <- data.frame(row.names = rownames(data)) |
63 data.factor = read.table( | 75 for (group in levels(data_factor$level)) { |
64 opt$factor, | 76 subcells <- as.data.frame(subset(data_factor, level == group, select = cellid)) |
65 check.names = FALSE, | 77 subdata <- as.data.frame(subset(data, select = as.vector(subcells$cellid))) |
66 header = TRUE, | 78 subdata_transformed <- transform(subdata, center = as.logical(opt$center), |
67 sep = '\t' | 79 scale = as.logical(opt$scale)) |
68 ) | 80 data_transformed <- cbind(data_transformed, subdata_transformed) |
69 colnames(data.factor) <- c("cellid", "level") | 81 } |
70 data.transformed <- data.frame(row.names=rownames(data), stringsAsFactors=FALSE) | |
71 for (group in levels(data.factor$level)){ | |
72 subcells <- as.data.frame(subset(data.factor, level==group, select=cellid)) | |
73 subdata <- as.data.frame(subset(data, select=as.vector(subcells$cellid))) | |
74 subdata.transformed <- transform(subdata, center=as.logical(opt$center), | |
75 scale=as.logical(opt$scale)) | |
76 data.transformed <- cbind(data.transformed, subdata.transformed) | |
77 } | |
78 } else { | 82 } else { |
79 data.transformed <- transform(data, center=as.logical(opt$center), | 83 data_transformed <- transform(data, center = as.logical(opt$center), |
80 scale=as.logical(opt$scale)) | 84 scale = as.logical(opt$scale)) |
81 } | 85 } |
82 | 86 |
83 | 87 |
84 write.table( | 88 write.table( |
85 cbind(gene=rownames(data.transformed), data.transformed), | 89 cbind(gene = rownames(data_transformed), data_transformed), |
86 opt$output, | 90 opt$output, |
87 col.names = TRUE, | 91 col.names = TRUE, |
88 row.names = FALSE, | 92 row.names = FALSE, |
89 quote = F, | 93 quote = FALSE, |
90 sep = "\t" | 94 sep = "\t" |
91 ) | 95 ) |
92 | |
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