# HG changeset patch # User artbio # Date 1508021582 14400 # Node ID 65befdc9925ae6f359f53c243ad78e222087682e # Parent 543472c9e272bf144376dae9e7a05d3daace5ba9 planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/mircounts commit 3514091150570410ce32cbdfda26fd6e5591fef1 diff -r 543472c9e272 -r 65befdc9925a mircounts.xml --- a/mircounts.xml Tue Sep 05 17:54:46 2017 -0400 +++ b/mircounts.xml Sat Oct 14 18:53:02 2017 -0400 @@ -1,17 +1,17 @@ - + Counts miRNA alignments from small RNA sequence data gnu-wget - bowtie - samtools - pysam + bowtie + samtools + pysam r-optparse r-lattice /dev/null | samtools sort -O bam -o '$output' && + bowtie-build hairpin.fa hairpin >/dev/null && + bowtie -v $v -M 1 --best --strata --norc -p \${GALAXY_SLOTS:-4} --sam hairpin -q clipped_input.fastq | samtools sort -O bam -o '$output' 2>&1 && samtools index $output && python '$__tool_directory__'/mircounts.py -pm --alignment $output --gff $gff3 --quality_threshold 10 --pre_mirs $pre_mir_count_file --mirs $mir_count_file --lattice $coverage_dataframe; #if $plotting.plottingOption == 'yes':