Mercurial > repos > artbio > oases
comparison oases_optimiser.xml @ 2:ab37eb09b4ca draft default tip
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/main/tools/oases commit d5a2de4b4c556e028fc5ef7ffcc7c2d0cf31c5ec
author | artbio |
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date | Mon, 12 Feb 2024 23:55:39 +0000 |
parents | c9b5ec6c45e8 |
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1:c9b5ec6c45e8 | 2:ab37eb09b4ca |
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1 <tool id="oasesoptimiserv" name="Oases_optimiser" version="1.3.0"> | 1 <tool id="oasesoptimiserv" name="Oases_optimiser" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> |
2 <description>Auto optimise de novo RNA-seq Oases/Velvet assembly</description> | 2 <description>Auto optimise de novo RNA-seq Oases/Velvet assembly</description> |
3 <macros> | |
4 <token name="@TOOL_VERSION@">1.4.0</token> | |
5 <token name="@VERSION_SUFFIX@">0</token> | |
6 <token name="@PROFILE@">23.0</token> | |
7 </macros> | |
3 <requirements> | 8 <requirements> |
4 <requirement type="package" version="0.2.09">oases</requirement> | 9 <requirement type="package" version="0.2.09">oases</requirement> |
5 <requirement type="package" version="1.2.10">velvet</requirement> | 10 <requirement type="package" version="1.2.10">velvet</requirement> |
6 <requirement type="package" version="3.7.6">python</requirement> | 11 <requirement type="package" version="3.12.1">python</requirement> |
7 </requirements> | 12 </requirements> |
8 <command><![CDATA[ | 13 <command><![CDATA[ |
9 python "$__tool_directory__"/oases_optimiser.py "$start_hash_length" "$end_hash_length" | 14 python '$__tool_directory__/oases_optimiser.py' '$start_hash_length' '$end_hash_length' |
10 #for $i in $inputs | 15 #for $i in $inputs |
11 "${i.input}" | 16 '${i.input}' |
12 #end for | 17 #end for |
13 "$transcripts" | 18 '$transcripts' |
14 ]]></command> | 19 ]]></command> |
15 <inputs> | 20 <inputs> |
16 <param label="Start Hash Length" name="start_hash_length" type="select" help="k-mer length in base pairs of the words being hashed. Shorter hash lengths (i.e. less than 31) may cause out-of-memory problems."> | 21 <param label="Start Hash Length" name="start_hash_length" type="select" help="k-mer length in base pairs of the words being hashed. Shorter hash lengths (i.e. less than 31) may cause out-of-memory problems."> |
17 <option value="11" selected="yes">11</option> | 22 <option value="11" selected="yes">11</option> |
18 <option value="13">13</option> | 23 <option value="13">13</option> |
19 <option value="15">15</option> | 24 <option value="15">15</option> |
57 <has_line_matching expression=">Locus_25_Transcript_1/1_Confidence_.+" /> | 62 <has_line_matching expression=">Locus_25_Transcript_1/1_Confidence_.+" /> |
58 </assert_contents> | 63 </assert_contents> |
59 </output> | 64 </output> |
60 </test> | 65 </test> |
61 </tests> | 66 </tests> |
62 <help> | 67 <help> |
63 **Oases Optimiser Overview** | 68 **Oases Optimiser Overview** |
64 | 69 |
65 Oases_ is a de novo transcriptome assembler specially designed to work with short reads. It is an extension of Velvet developed by Daniel Zerbino and Ewan Birney at the European Bioinformatics Institute (EMBL-EBI), near Cambridge, in the United Kingdom. | 70 Oases_ is a de novo transcriptome assembler specially designed to work with short reads. It is an extension of Velvet developed by Daniel Zerbino and Ewan Birney at the European Bioinformatics Institute (EMBL-EBI), near Cambridge, in the United Kingdom. |
66 | 71 |
67 Provide all the information about insert lengths and their standard deviation as | 72 Provide all the information about insert lengths and their standard deviation as |
71 | 76 |
72 Oases produces a number of output files, which correspond to the different algorithms | 77 Oases produces a number of output files, which correspond to the different algorithms |
73 being run succesively on the data. In the above example, you would find: | 78 being run succesively on the data. In the above example, you would find: |
74 | 79 |
75 transcripts.fa | 80 transcripts.fa |
76 A FASTA file containing the transcripts imputed directly from trivial | 81 A FASTA file containing the transcripts imputed directly from trivial |
77 clusters of contigs (loci with less than two transcripts and Confidence Values = 1) | 82 clusters of contigs (loci with less than two transcripts and Confidence Values = 1) |
78 and the highly expressed transcripts imputed by dynamic | 83 and the highly expressed transcripts imputed by dynamic |
79 programming (loci with more than 2 transcripts and Confidence Values less than 1). | 84 programming (loci with more than 2 transcripts and Confidence Values less than 1). |
80 | 85 |
81 splicing_events.txt | 86 splicing_events.txt |
82 A hybrid file which describes the contigs contained in each locus in FASTA | 87 A hybrid file which describes the contigs contained in each locus in FASTA |
83 format, interlaced with one line descriptions of splicing events using the | 88 format, interlaced with one line descriptions of splicing events using the |
84 AStalavista nomenclature*. | 89 AStalavista nomenclature*. |
85 | 90 |
86 Read the Oases `documentation`__ for details on using the Oases Assembler. | 91 Read the Oases `documentation`__ for details on using the Oases Assembler. |
87 | 92 |
88 .. _Oases: http://www.ebi.ac.uk/~zerbino/oases/ | 93 .. _Oases: https://github.com/dzerbino/oases |
89 | 94 |
90 .. __: http://www.ebi.ac.uk/~zerbino/oases/Manual.txt | 95 .. __: https://bioweb.pasteur.fr/docs/modules/oases/0.2.08/OasesManual.pdf |
91 | 96 |
92 ------ | 97 ------ |
93 | 98 |
94 **Other outputs (as per Velvet)** | 99 **Other outputs (as per Velvet)** |
95 | 100 |