annotate rsem-bwt2.xml @ 0:e5e836936d60 draft

planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
author artbio
date Sat, 31 Mar 2018 21:30:07 -0400
parents
children 49795544dac7
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0
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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1 <tool id="rsembowtie2" name="RSEM-Bowtie2" version="0.4.0">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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2 <description></description>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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3 <macros>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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4 <import>macros.xml</import>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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5 </macros>
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6 <requirements>
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7 <requirement type="package" version="1.3.0">rsem</requirement>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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8 <requirement type="package" version="2.3.4">bowtie2</requirement>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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9 </requirements>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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10 <stdio>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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11 <exit_code range="1:" level="warning" description="Tool exception" />
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12 </stdio>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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13 <command detect_errors="exit_code"><![CDATA[
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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14 #if $job.select_job == "index":
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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15 echo ${job.reference_name} " " | tee $reference_file &&
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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16 mkdir $reference_file.files_path &&
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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17 rsem-prepare-reference
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18 #if $job.polya.polya_use == 'add':
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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19 #if $job.polya.polya_length:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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20 --polyA-length $job.polya.polya_length
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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21 #end if
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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22 #elif $job.polya.polya_use == 'subset':
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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23 --no-polyA-subset $job.polya.no_polya_subset
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24 #if $job.polya.polya_length:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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25 --polyA-length $job.polya.polya_length
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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26 #end if
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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27 #elif $job.polya.polya_use == 'none':
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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28 --no-polyA
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29 #end if
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30 $job.ntog
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31 #if $job.transcript_to_gene_map:
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32 --transcript-to-gene-map $job.transcript_to_gene_map
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33 #end if
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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34 --bowtie2
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35 #if $job.self_reference.ref_type == 'transcripts':
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36 $job.self_reference.reference_fasta_file
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37 #else:
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38 --gtf $job.self_reference.gtf
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39 $job.self_reference.reference_fasta_file
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40 #end if
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41 ${reference_file.files_path}/${job.reference_name}
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42 > ${reference_file.files_path}/${job.reference_name}.log
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43 #end if
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44
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45 #if $job.select_job == "index" and $run_rsem.select == "Yes":
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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46 &&
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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47 #end if
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48
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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49 #if $run_rsem.select == "Yes":
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50 ## uncompress fastq.gz or fastqsanger.gz if needed
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51 #if $run_rsem.input.fastq.matepair=="single":
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52 #if $run_rsem.input.fastq.singlefastq.is_of_type('fastq.gz') or $run_rsem.input.fastq.singlefastq.is_of_type('fastqsanger.gz'):
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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53 gunzip < '$run_rsem.input.fastq.singlefastq' > uncomp_single.fastq &&
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54 #elif $run_rsem.input.fastq.singlefastq.is_of_type('fastq') or $run_rsem.input.fastq.singlefastq.is_of_type('fastqsanger'):
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55 ln -f -s '$run_rsem.input.fastq.singlefastq' 'uncomp_single.fastq' &&
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56 #end if
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57 #elif $run_rsem.input.fastq.matepair=="paired":
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58 #if $run_rsem.input.fastq.fastq1.is_of_type('fastq.gz') or $run_rsem.input.fastq.fastq1.is_of_type('fastqsanger.gz'):
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59 gunzip < '$run_rsem.input.fastq.fastq1' > uncomp_pair1.fastq &&
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60 gunzip < '$run_rsem.input.fastq.fastq2' > uncomp_pair2.fastq &&
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61 #elif $run_rsem.input.fastq.singlefastq.is_of_type('fastq') or $run_rsem.input.fastq.singlefastq.is_of_type('fastqsanger'):
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62 ln -f -s '$run_rsem.input.fastq.fastq1' 'uncomp_pair1.fastq' &&
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63 ln -f -s '$run_rsem.input.fastq.fastq2' 'uncomp_pair2.fastq' &&
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64 #end if
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65 #end if
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66 rsem-calculate-expression
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67 ## --tag string
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68 #if $run_rsem.seedlength:
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69 --seed-length $run_rsem.seedlength
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70 #end if
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71 --forward-prob $run_rsem.forward_prob
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72 #if $run_rsem.rsem_options.fullparams == 'fullset':
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73 ## Fragment info
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74 #if $run_rsem.rsem_options.fragment_length_mean:
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75 --fragment-length-mean $run_rsem.rsem_options.fragment_length_mean
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76 #end if
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77 #if $run_rsem.rsem_options.fragment_length_min:
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78 --fragment-length-min $run_rsem.rsem_options.fragment_length_min
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79 #end if
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80 #if $run_rsem.rsem_options.fragment_length_sd:
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81 --fragment-length-sd $run_rsem.rsem_options.fragment_length_sd
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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82 #end if
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83 #if $run_rsem.rsem_options.fragment_length_max:
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84 --fragment-length-max $run_rsem.rsem_options.fragment_length_max
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85 #end if
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86 ## RSPD
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87 #if $run_rsem.rsem_options.rspd.estimate == 'yes':
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88 --estimate-rspd
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89 #if $run_rsem.rsem_options.rspd.num_rspd_bins:
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90 --num-rspd-bins $run_rsem.rsem_options.rspd.num_rspd_bins
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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91 #end if
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92 #end if
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93 ## Calculate 95% credibility intervals and posterior mean estimates.
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94 #if $run_rsem.rsem_options.useci.ci == 'yes':
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95 --calc-ci
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96 #if $run_rsem.rsem_options.useci.cimem:
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97 --ci-memory $run_rsem.rsem_options.useci.cimem
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98 #end if
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99 #end if
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100 #end if
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101 --num-threads \${GALAXY_SLOTS:-4}
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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102 --bowtie2
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103 #if $run_rsem.input.format == 'fasta' and $run_rsem.input.bowtie2_options.fullparams == 'fullset':
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104 ## Bowtie params
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105 #if $run_rsem.input.bowtie2_options.bowtie2_mismatch_rate:
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106 --bowtie2-mismatch-rate $run_rsem.input.bowtie2_options.bowtie2_mismatch_rate
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107 #end if
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108 #if $run_rsem.input.bowtie2_options.bowtie2_k:
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109 --bowtie2-k $run_rsem.input.bowtie2_options.bowtie2_k
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110 #end if
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111 #if $run_rsem.input.bowtie2_options.bowtie2_sensitivity_level:
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112 --bowtie2-sensitivity-level $run_rsem.input.bowtie2_options.bowtie2_sensitivity_level
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113 #end if
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114 #end if
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115 ## Outputs
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116 #if $run_rsem.rsem_outputs.result_bams == 'none':
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117 --no-bam-output
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118 #elif $run_rsem.rsem_outputs.result_bams == 'default':
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119 --sort-bam-by-coordinate
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120 #else
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121 --sort-bam-by-coordinate
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122 --output-genome-bam
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123 $run_rsem.rsem_outputs.sampling_for_bam
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124 #end if
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125 ## Input data
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126 #if $run_rsem.input.format=="fastq"
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127 $run_rsem.input.fastq_select
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128 #if $run_rsem.input.fastq.matepair=="single":
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129 uncomp_single.fastq
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130 #elif $run_rsem.input.fastq.matepair=="paired":
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131 --paired-end
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132 uncomp_pair1.fastq
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133 uncomp_pair2.fastq
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134 #end if
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135 #elif $run_rsem.input.format=="fasta"
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136 --no-qualities
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137 #if $run_rsem.input.fasta.matepair=="single":
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138 $run_rsem.input.fasta.singlefasta
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139 #elif $run_rsem.input.fasta.matepair=="paired":
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140 --paired-end
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141 $run_rsem.input.fasta.fasta1
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142 $run_rsem.input.fasta.fasta2
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143 #end if
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144 #elif $run_rsem.input.format=="sam"
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145 #if $run_rsem.input.matepair=="paired":
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146 --paired-end
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147 #end if
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148 #if $run_rsem.input.rsem_sam._extension == 'sam':
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149 --sam
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150 #elif $run_rsem.input.rsem_sam._extension == 'bam':
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151 --bam
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152 #end if
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153 $run_rsem.input.rsem_sam
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154 #end if
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155 ## RSEM reference
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156 #if $run_rsem.reference.refSrc == 'history':
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157 ${run_rsem.reference.rsem_ref.extra_files_path}/${run_rsem.reference.rsem_ref.metadata.reference_name}
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158 #elif $run_rsem.reference.refSrc == 'self':
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159 ${reference_file.files_path}/${job.reference_name}
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160 #end if
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161 ## sample_name: use a hard coded name so we can pull out galaxy outputs
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162 rsem_output
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163 ## direct output into logfile
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164 > $log
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165 #end if
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166 ]]></command>
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167
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168 <inputs>
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169 <conditional name="job">
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170 <param name="select_job" type="select" label="rsem reference">
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171 <option value="index">Build rsem reference</option>
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172 <option value="no-index" selected="true">rsem reference available from history</option>
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173 </param>
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174 <when value="index">
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175 <conditional name="self_reference">
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176 <param name="ref_type" type="select" label="Reference transcript source">
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177 <option value="transcripts">transcript fasta</option>
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178 <option value="genomic">reference genome and gtf</option>
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179 </param>
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180 <when value="transcripts">
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181 <param name="reference_fasta_file" type="data" format="fasta" label="reference fasta file"
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182 help="The files should contain the sequences of transcripts."/>
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183 </when>
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184 <when value="genomic">
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185 <param name="reference_fasta_file" type="data" format="fasta" label="reference fasta file"
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186 help="The file should contain the sequence of an entire genome."/>
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187 <param name="gtf" type="data" format="gtf" label="gtf"
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188 help="extract transcript reference sequences using the gene annotations specified in this GTF" />
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189 </when>
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190 </conditional>
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191 <param name="transcript_to_gene_map" type="data" format="tabular" optional="true" label="Map of gene ids to transcript (isoform) ids" >
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192 <help>
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193 Each line of should be of the form: gene_id transcript_id ( with the two fields separated by a tab character )
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194 The map can be obtained from the UCSC table browser
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195 group: Genes and Gene Prediction Tracks
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196 table: knownIsoforms
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197 Without a map:
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198 If a reference genome and gtf is used, then RSEM uses the "gene_id" and "transcript_id" attributes in the GTF file.
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199 Otherwise, RSEM assumes that each sequence in the reference sequence files is a separate gene.
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200 </help>
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201 </param>
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202 <param name="reference_name" type="text" value="rsem_ref_name" label="reference name">
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203 <help>A one word name for this RSEM reference containing only letters, digits, and underscore characters</help>
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204 <validator type="regex" message="Use only letters, digits, and underscore characters">^\w+$</validator>
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205 </param>
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206 <conditional name="polya">
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207 <param name="polya_use" type="select" label="PolyA ">
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208 <option value="add" selected="true">Add poly(A) tails to all transcripts</option>
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209 <option value="subset">Exclude poly(A) tails from selected transcripts</option>
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210 <option value="none">Do not add poly(A) tails to any transcripts</option>
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211 </param>
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212 <when value="add">
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213 <param name="polya_length" type="integer" value="125" optional="true" label="The length of the poly(A) tails to be added. (Default: 125)">
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214 <validator type="in_range" message="must be positive " min="1"/>
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215 </param>
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216 </when>
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217 <when value="subset">
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218 <param name="no_polya_subset" type="data" format="tabular" optional="true" label="List of transcript IDs (one per line) that should should not have polyA tails added."/>
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219 <param name="polya_length" type="integer" value="125" optional="true" label="The length of the poly(A) tails to be added. (Default: 125)">
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220 <validator type="in_range" message="must be positive " min="1"/>
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221 </param>
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222 </when>
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223 <when value="none"/>
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224 </conditional>
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225 <param name="ntog" type="boolean" truevalue="--no-ntog" falsevalue="" checked="false" label="Disable the conversion of 'N' characters to 'G' characters in the reference sequences" help="Bowtie uses the automatic N to G conversion to align against all positions in the reference."/>
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226 </when>
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227 <when value="no-index">
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228 </when>
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229 </conditional>
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230
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231 <conditional name="run_rsem">
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232 <param name="select" type="select" label="calculate expression with rsem">
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233 <option value="No">Just build rsem reference for latter rsem profiling</option>
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234 <option value="Yes" selected="true">profile expression with rsem</option>
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235 </param>
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236 <when value="Yes">
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237 <param name="sample" type="text" value="rsem_sample" label="Sample name" />
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238 <conditional name="reference">
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239 <param name="refSrc" type="select" label="RSEM Reference Source">
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240 <option value="history">From your history</option>
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241 <option value="self">Prepare RSEM Reference with this tool</option>
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242 </param>
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243 <when value="history">
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244 <param name="rsem_ref" type="data" format="rsem_ref" label="RSEM reference" />
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245 </when>
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246 <when value="self">
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247 </when>
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248 </conditional>
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249 <conditional name="input">
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250 <param name="format" type="select" label="RSEM Input file type">
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251 <option value="fastq">FASTQ</option>
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252 <option value="fasta">FASTA</option>
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253 <option value="sam">SAM/BAM</option>
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254 </param>
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255 <when value="fastq">
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256 <param name="fastq_select" size="15" type="select" label="FASTQ type" >
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257 <option value="--phred33-quals" selected="true">phred33 qualities (default for sanger)</option>
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258 <option value="--solexa-quals">solexa qualities</option>
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259 <option value="--phred64-quals">phred64 qualities</option>
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260 </param>
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261 <conditional name="fastq">
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262 <param name="matepair" type="select" label="Library type">
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263 <option value="single">Single End Reads</option>
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264 <option value="paired">Paired End Reads</option>
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265 </param>
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266 <when value="single">
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267 <param name="singlefastq" type="data" format="fastq,fastq.gz" label="FASTQ file" />
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268 </when>
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269 <when value="paired">
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270 <param name="fastq1" type="data" format="fastq,fastq.gz" label="Read 1 fastq file" />
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271 <param name="fastq2" type="data" format="fastq,fastq.gz" label="Read 2 fastq file" />
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272 </when>
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273 </conditional>
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274 <expand macro="bowtie2_options"/>
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275 </when>
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276 <when value="fasta">
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277 <conditional name="fasta">
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278 <param name="matepair" type="select" label="Library Type">
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279 <option value="single">Single End Reads</option>
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280 <option value="paired">Paired End Reads</option>
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281 </param>
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282 <when value="single">
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283 <param name="singlefasta" type="data" format="fasta" label="fasta file" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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284 </when>
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artbio
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285 <when value="paired">
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artbio
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286 <param name="fasta1" type="data" format="fasta" label="Read 1 fasta file" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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287 <param name="fasta2" type="data" format="fasta" label="Read 2 fasta file" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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288 </when>
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289 </conditional>
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artbio
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290 <expand macro="bowtie2_options"/>
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291 </when>
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292 <when value="sam">
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293 <!-- convert-sam-for-rsem /ref/mouse_125 input.sam -o input_for_rsem.sam -->
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294 <param name="matepair" type="select" label="Library Type">
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295 <option value="single">Single End Reads</option>
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artbio
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296 <option value="paired">Paired End Reads</option>
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297 </param>
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298 <param name="rsem_sam" type="data" format="rsem_sam" label="RSEM formatted SAM file" />
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299 </when>
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300 </conditional>
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301 <expand macro="rsem_options"/>
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artbio
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302 <conditional name="rsem_outputs">
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303 <param name="result_bams" type="select" label="Create bam results files"
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304 help="In addition to the transcript-coordinate-based BAM file output, also output a BAM file with the read alignments in genomic coordinates" >
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305 <option value="none">No BAM results files</option>
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306 <option value="default" selected="true">Transcript BAM results file</option>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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307 <option value="both">Transcript and genome BAM results files</option>
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308 </param>
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artbio
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309 <when value="none"/>
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310 <when value="default">
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artbio
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311 <expand macro="sampling_for_bam"/>
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312 </when>
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313 <when value="both">
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artbio
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314 <expand macro="sampling_for_bam"/>
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315 </when>
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316 </conditional>
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317 </when>
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artbio
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318 <when value="No">
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319 </when>
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320 </conditional>
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artbio
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321 </inputs>
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322
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artbio
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323 <outputs>
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artbio
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324 <data format="rsem_ref" name="reference_file" label="RSEM ${job.reference_name} reference">
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artbio
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325 <filter>job['select_job'] == "index"</filter>
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326 </data>
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327 <data format="tabular" name="gene_abundances" label="${run_rsem.sample}.gene_abundances" from_work_dir="rsem_output.genes.results">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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328 <filter>run_rsem['select'] == "Yes"</filter>
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329 </data>
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330 <data format="tabular" name="isoform_abundances" label="${run_rsem.sample}.isoform_abundances" from_work_dir="rsem_output.isoforms.results">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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331 <filter>run_rsem['select'] == "Yes"</filter>
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332 </data>
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333 <data format="bam" name="transcript_sorted_bam" label="${run_rsem.sample}.transcript.bam" from_work_dir="rsem_output.transcript.sorted.bam" >
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334 <filter>run_rsem['select'] == "Yes" and run_rsem['rsem_outputs']['result_bams'] != "none"</filter>
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335 </data>
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336 <data format="bam" name="genome_sorted_bam" label="${run_rsem.sample}.genome.bam" from_work_dir="rsem_output.genome.sorted.bam">
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337 <filter>run_rsem['select'] == "Yes" and run_rsem['rsem_outputs']['result_bams'] == "both"</filter>
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338 </data>
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339 <data format="txt" name="log" label="${run_rsem.sample}.rsem_log">
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340 <filter>run_rsem['select'] == "Yes"</filter>
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341 </data>
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342 </outputs>
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artbio
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343
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344 <tests>
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345 <test>
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346 <param name="select_job" value="index"/>
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347 <param name="ref_type" value="genomic"/>
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348 <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/>
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349 <param name="gtf" value="ref.gtf" ftype="gtf"/>
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350 <param name="reference_name" value="ref"/>
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351 <param name="select" value="Yes"/>
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352 <param name="sample" value="rsem_sample"/>
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353 <param name="refSrc" value="self"/>
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354 <param name="format" value="fastq"/>
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artbio
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355 <param name="matepair" value="single"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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356 <param name="singlefastq" value="test.fq" ftype="fastqsanger"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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357 <param name="result_bams" value="none"/>
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358 <output name="reference_file">
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artbio
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359 <assert_contents>
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360 <has_text text="ref" />
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361 </assert_contents>
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diff changeset
362 </output>
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artbio
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diff changeset
363 <output name="gene_abundances" value="gene_abundances.tab2"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
364 <output name="isoform_abundances" value="isoform_abundances.tab2" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
365 <output name="log">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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366 <assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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367 <has_text text="Expression Results are written" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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368 </assert_contents>
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369 </output>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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370 </test>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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371 <test>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
372 <param name="select_job" value="index"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
373 <param name="ref_type" value="genomic"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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374 <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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375 <param name="gtf" value="ref.gtf" ftype="gtf"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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376 <param name="reference_name" value="ref"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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377 <param name="select" value="Yes"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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378 <param name="sample" value="rsem_sample"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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379 <param name="refSrc" value="self"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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380 <param name="format" value="fastq"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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381 <param name="matepair" value="single"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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382 <param name="singlefastq" value="test.fastq.gz" ftype="fastqsanger.gz"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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383 <param name="result_bams" value="none"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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384 <output name="reference_file">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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385 <assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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386 <has_text text="ref" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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387 </assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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388 </output>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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389 <output name="gene_abundances" value="gene_abundances.tab2"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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390 <output name="isoform_abundances" value="isoform_abundances.tab2" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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391 <output name="log">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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392 <assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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393 <has_text text="Expression Results are written" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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394 </assert_contents>
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395 </output>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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396 </test>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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397 <test>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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398 <param name="select_job" value="index"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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399 <param name="ref_type" value="genomic"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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400 <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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401 <param name="gtf" value="ref.gtf" ftype="gtf"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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402 <param name="reference_name" value="ref"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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403 <param name="select" value="No"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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404 <output name="reference_file">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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405 <assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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406 <has_text text="ref" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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407 </assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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408 </output>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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409 </test>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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410 <test>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
411 <param name="select_job" value="index"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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412 <param name="ref_type" value="genomic"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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413 <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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414 <param name="gtf" value="ref.gtf" ftype="gtf"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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415 <param name="reference_name" value="ref"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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416 <param name="select" value="No"/>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
417 <output name="reference_file">
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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418 <assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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419 <has_text text="ref" />
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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420 </assert_contents>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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421 </output>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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422 </test>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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423 </tests>
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artbio
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424
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
425 <help>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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426 .. class:: infomark
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artbio
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diff changeset
427
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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428 RSEM HOME PAGE - http://deweylab.biostat.wisc.edu/rsem/
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artbio
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diff changeset
429
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
430 NAME
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
431 rsem-prepare-reference
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artbio
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diff changeset
432
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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433 SYNOPSIS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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434 rsem-prepare-reference [options] reference_fasta_file(s) reference_name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
435
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
436 DESCRIPTION
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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437 The rsem-prepare-reference program extracts/preprocesses the reference sequences and builds Bowtie indices using default parameters.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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438 This program is used in conjunction with the 'rsem-calculate-expression' program.
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artbio
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diff changeset
439
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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diff changeset
440 INPUTS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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441 A fasta file of transcripts
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
442 or
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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443 A genome sequence fasta file and a GTF gene annotation file. (When using UCSC data, include the related knownIsoforms.txt)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
444
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
445 ---
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
446
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
447 NAME
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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448 rsem-calculate-expression - Estimate gene and isoform expression from
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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449 RNA-Seq data.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
450
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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451 SYNOPSIS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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452 rsem-calculate-expression [options] upstream_read_file(s) reference_name sample_name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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453 rsem-calculate-expression [options] --paired-end upstream_read_file(s) downstream_read_file(s) reference_name sample_name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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454 rsem-calculate-expression [options] --alignments [--paired-end] input reference_name sample_name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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diff changeset
455
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
456 ARGUMENTS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
457 upstream_read_files(s)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
458 Comma-separated list of files containing single-end reads or
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
459 upstream reads for paired-end data. By default, these files are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
460 assumed to be in FASTQ format. If the --no-qualities option is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
461 specified, then FASTA format is expected.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
462
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
463 downstream_read_file(s)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
464 Comma-separated list of files containing downstream reads which are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
465 paired with the upstream reads. By default, these files are assumed
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
466 to be in FASTQ format. If the --no-qualities option is specified,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
467 then FASTA format is expected.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
468
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
469 input
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
470 SAM/BAM/CRAM formatted input file. If "-" is specified for the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
471 filename, the input is instead assumed to come from standard input.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
472 RSEM requires all alignments of the same read group together. For
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
473 paired-end reads, RSEM also requires the two mates of any alignment
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
474 be adjacent. In addition, RSEM does not allow the SEQ and QUAL
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
475 fields to be empty. See Description section for how to make input
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
476 file obey RSEM's requirements.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
477
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
478 reference_name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
479 The name of the reference used. The user must have run
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
480 'rsem-prepare-reference' with this reference_name before running
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
481 this program.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
482
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
483 sample_name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
484 The name of the sample analyzed. All output files are prefixed by
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
485 this name (e.g., sample_name.genes.results)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
486
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
487 BASIC OPTIONS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
488 --paired-end
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
489 Input reads are paired-end reads. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
490
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
491 --no-qualities
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
492 Input reads do not contain quality scores. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
493
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
494 --strandedness &lt;none|forward|reverse&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
495 This option defines the strandedness of the RNA-Seq reads. It
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
496 recognizes three values: 'none', 'forward', and 'reverse'. 'none'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
497 refers to non-strand-specific protocols. 'forward' means all
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
498 (upstream) reads are derived from the forward strand. 'reverse'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
499 means all (upstream) reads are derived from the reverse strand. If
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
500 'forward'/'reverse' is set, the '--norc'/'--nofw' Bowtie/Bowtie 2
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
501 option will also be enabled to avoid aligning reads to the opposite
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
502 strand. For Illumina TruSeq Stranded protocols, please use
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
503 'reverse'. (Default: 'none')
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
504
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
505 -p/--num-threads &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
506 Number of threads to use. Both Bowtie/Bowtie2, expression estimation
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
507 and 'samtools sort' will use this many threads. (Default: 1)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
508
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
509 --alignments
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
510 Input file contains alignments in SAM/BAM/CRAM format. The exact
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
511 file format will be determined automatically. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
512
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
513 --fai &lt;file&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
514 If the header section of input alignment file does not contain
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
515 reference sequence information, this option should be turned on.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
516 &lt;file&gt; is a FAI format file containing each reference sequence's
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
517 name and length. Please refer to the SAM official website for the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
518 details of FAI format. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
519
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
520 --bowtie2
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
521 Use Bowtie 2 instead of Bowtie to align reads. Since currently RSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
522 does not handle indel, local and discordant alignments, the Bowtie2
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
523 parameters are set in a way to avoid those alignments. In
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
524 particular, we use options '--sensitive --dpad 0 --gbar 99999999
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
525 --mp 1,1 --np 1 --score-min L,0,-0.1' by default. The last parameter
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
526 of '--score-min', '-0.1', is the negative of maximum mismatch rate.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
527 This rate can be set by option '--bowtie2-mismatch-rate'. If reads
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
528 are paired-end, we additionally use options '--no-mixed' and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
529 '--no-discordant'. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
530
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
531 --star
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
532 Use STAR to align reads. Alignment parameters are from ENCODE3's
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
533 STAR-RSEM pipeline. To save computational time and memory resources,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
534 STAR's Output BAM file is unsorted. It is stored in RSEM's temporary
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
535 directory with name as 'sample_name.bam'. Each STAR job will have
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
536 its own private copy of the genome in memory. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
537
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
538 --append-names
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
539 If gene_name/transcript_name is available, append it to the end of
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
540 gene_id/transcript_id (separated by '_') in files
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
541 'sample_name.isoforms.results' and 'sample_name.genes.results'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
542 (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
543
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
544 --seed &lt;uint32&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
545 Set the seed for the random number generators used in calculating
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
546 posterior mean estimates and credibility intervals. The seed must be
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
547 a non-negative 32 bit integer. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
548
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
549 --single-cell-prior
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
550 By default, RSEM uses Dirichlet(1) as the prior to calculate
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
551 posterior mean estimates and credibility intervals. However, much
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
552 less genes are expressed in single cell RNA-Seq data. Thus, if you
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
553 want to compute posterior mean estimates and/or credibility
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
554 intervals and you have single-cell RNA-Seq data, you are recommended
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
555 to turn on this option. Then RSEM will use Dirichlet(0.1) as the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
556 prior which encourage the sparsity of the expression levels.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
557 (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
558
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
559 --calc-pme
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
560 Run RSEM's collapsed Gibbs sampler to calculate posterior mean
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
561 estimates. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
562
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
563 --calc-ci
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
564 Calculate 95% credibility intervals and posterior mean estimates.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
565 The credibility level can be changed by setting
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
566 '--ci-credibility-level'. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
567
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
568 -q/--quiet
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
569 Suppress the output of logging information. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
570
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
571 -h/--help
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
572 Show help information.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
573
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
574 --version
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
575 Show version information.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
576
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
577 OUTPUT OPTIONS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
578 --sort-bam-by-read-name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
579 Sort BAM file aligned under transcript coordidate by read name.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
580 Setting this option on will produce deterministic maximum likelihood
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
581 estimations from independent runs. Note that sorting will take long
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
582 time and lots of memory. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
583
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
584 --no-bam-output
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
585 Do not output any BAM file. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
586
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
587 --sampling-for-bam
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
588 When RSEM generates a BAM file, instead of outputting all alignments
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
589 a read has with their posterior probabilities, one alignment is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
590 sampled according to the posterior probabilities. The sampling
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
591 procedure includes the alignment to the "noise" transcript, which
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
592 does not appear in the BAM file. Only the sampled alignment has a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
593 weight of 1. All other alignments have weight 0. If the "noise"
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
594 transcript is sampled, all alignments appeared in the BAM file
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
595 should have weight 0. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
596
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
597 --output-genome-bam
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
598 Generate a BAM file, 'sample_name.genome.bam', with alignments
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
599 mapped to genomic coordinates and annotated with their posterior
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
600 probabilities. In addition, RSEM will call samtools (included in
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
601 RSEM package) to sort and index the bam file.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
602 'sample_name.genome.sorted.bam' and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
603 'sample_name.genome.sorted.bam.bai' will be generated. (Default:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
604 off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
605
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
606 --sort-bam-by-coordinate
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
607 Sort RSEM generated transcript and genome BAM files by coordinates
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
608 and build associated indices. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
609
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
610 --sort-bam-memory-per-thread &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
611 Set the maximum memory per thread that can be used by 'samtools
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
612 sort'. &lt;string&gt; represents the memory and accepts suffices 'K/M/G'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
613 RSEM will pass &lt;string&gt; to the '-m' option of 'samtools sort'. Note
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
614 that the default used here is different from the default used by
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
615 samtools. (Default: 1G)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
616
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
617 ALIGNER OPTIONS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
618 --seed-length &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
619 Seed length used by the read aligner. Providing the correct value is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
620 important for RSEM. If RSEM runs Bowtie, it uses this value for
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
621 Bowtie's seed length parameter. Any read with its or at least one of
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
622 its mates' (for paired-end reads) length less than this value will
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
623 be ignored. If the references are not added poly(A) tails, the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
624 minimum allowed value is 5, otherwise, the minimum allowed value is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
625 25. Note that this script will only check if the value &gt;= 5 and give
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
626 a warning message if the value &lt; 25 but &gt;= 5. (Default: 25)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
627
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
628 --phred33-quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
629 Input quality scores are encoded as Phred+33. (Default: on)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
630
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
631 --phred64-quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
632 Input quality scores are encoded as Phred+64 (default for GA
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
633 Pipeline ver. &gt;= 1.3). (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
634
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
635 --solexa-quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
636 Input quality scores are solexa encoded (from GA Pipeline ver. &lt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
637 1.3). (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
638
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
639 --bowtie-path &lt;path&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
640 The path to the Bowtie executables. (Default: the path to the Bowtie
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
641 executables is assumed to be in the user's PATH environment
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
642 variable)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
643
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
644 --bowtie-n &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
645 (Bowtie parameter) max # of mismatches in the seed. (Range: 0-3,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
646 Default: 2)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
647
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
648 --bowtie-e &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
649 (Bowtie parameter) max sum of mismatch quality scores across the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
650 alignment. (Default: 99999999)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
651
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
652 --bowtie-m &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
653 (Bowtie parameter) suppress all alignments for a read if &gt; &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
654 valid alignments exist. (Default: 200)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
655
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
656 --bowtie-chunkmbs &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
657 (Bowtie parameter) memory allocated for best first alignment
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
658 calculation (Default: 0 - use Bowtie's default)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
659
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
660 --bowtie2-path &lt;path&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
661 (Bowtie 2 parameter) The path to the Bowtie 2 executables. (Default:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
662 the path to the Bowtie 2 executables is assumed to be in the user's
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
663 PATH environment variable)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
664
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
665 --bowtie2-mismatch-rate &lt;double&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
666 (Bowtie 2 parameter) The maximum mismatch rate allowed. (Default:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
667 0.1)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
668
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
669 --bowtie2-k &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
670 (Bowtie 2 parameter) Find up to &lt;int&gt; alignments per read. (Default:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
671 200)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
672
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
673 --bowtie2-sensitivity-level &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
674 (Bowtie 2 parameter) Set Bowtie 2's preset options in --end-to-end
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
675 mode. This option controls how hard Bowtie 2 tries to find
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
676 alignments. &lt;string&gt; must be one of "very_fast", "fast", "sensitive"
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
677 and "very_sensitive". The four candidates correspond to Bowtie 2's
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
678 "--very-fast", "--fast", "--sensitive" and "--very-sensitive"
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
679 options. (Default: "sensitive" - use Bowtie 2's default)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
680
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
681 --star-path &lt;path&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
682 The path to STAR's executable. (Default: the path to STAR executable
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
683 is assumed to be in user's PATH environment variable)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
684
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
685 --star-gzipped-read-file
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
686 (STAR parameter) Input read file(s) is compressed by gzip. (Default:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
687 off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
688
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
689 --star-bzipped-read-file
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
690 (STAR parameter) Input read file(s) is compressed by bzip2.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
691 (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
692
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
693 --star-output-genome-bam
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
694 (STAR parameter) Save the BAM file from STAR alignment under genomic
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
695 coordinate to 'sample_name.STAR.genome.bam'. This file is NOT sorted
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
696 by genomic coordinate. In this file, according to STAR's manual,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
697 'paired ends of an alignment are always adjacent, and multiple
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
698 alignments of a read are adjacent as well'. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
699
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
700 ADVANCED OPTIONS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
701 --tag &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
702 The name of the optional field used in the SAM input for identifying
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
703 a read with too many valid alignments. The field should have the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
704 format &lt;tagName&gt;:i:&lt;value&gt;, where a &lt;value&gt; bigger than 0 indicates
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
705 a read with too many alignments. (Default: "")
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
706
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
707 --fragment-length-min &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
708 Minimum read/insert length allowed. This is also the value for the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
709 Bowtie/Bowtie2 -I option. (Default: 1)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
710
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
711 --fragment-length-max &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
712 Maximum read/insert length allowed. This is also the value for the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
713 Bowtie/Bowtie 2 -X option. (Default: 1000)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
714
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
715 --fragment-length-mean &lt;double&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
716 (single-end data only) The mean of the fragment length distribution,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
717 which is assumed to be a Gaussian. (Default: -1, which disables use
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
718 of the fragment length distribution)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
719
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
720 --fragment-length-sd &lt;double&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
721 (single-end data only) The standard deviation of the fragment length
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
722 distribution, which is assumed to be a Gaussian. (Default: 0, which
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
723 assumes that all fragments are of the same length, given by the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
724 rounded value of --fragment-length-mean)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
725
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
726 --estimate-rspd
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
727 Set this option if you want to estimate the read start position
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
728 distribution (RSPD) from data. Otherwise, RSEM will use a uniform
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
729 RSPD. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
730
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
731 --num-rspd-bins &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
732 Number of bins in the RSPD. Only relevant when '--estimate-rspd' is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
733 specified. Use of the default setting is recommended. (Default: 20)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
734
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
735 --gibbs-burnin &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
736 The number of burn-in rounds for RSEM's Gibbs sampler. Each round
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
737 passes over the entire data set once. If RSEM can use multiple
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
738 threads, multiple Gibbs samplers will start at the same time and all
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
739 samplers share the same burn-in number. (Default: 200)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
740
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
741 --gibbs-number-of-samples &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
742 The total number of count vectors RSEM will collect from its Gibbs
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
743 samplers. (Default: 1000)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
744
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
745 --gibbs-sampling-gap &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
746 The number of rounds between two succinct count vectors RSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
747 collects. If the count vector after round N is collected, the count
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
748 vector after round N + &lt;int&gt; will also be collected. (Default: 1)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
749
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
750 --ci-credibility-level &lt;double&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
751 The credibility level for credibility intervals. (Default: 0.95)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
752
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
753 --ci-memory &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
754 Maximum size (in memory, MB) of the auxiliary buffer used for
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
755 computing credibility intervals (CI). (Default: 1024)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
756
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
757 --ci-number-of-samples-per-count-vector &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
758 The number of read generating probability vectors sampled per
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
759 sampled count vector. The crebility intervals are calculated by
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
760 first sampling P(C | D) and then sampling P(Theta | C) for each
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
761 sampled count vector. This option controls how many Theta vectors
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
762 are sampled per sampled count vector. (Default: 50)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
763
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
764 --keep-intermediate-files
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
765 Keep temporary files generated by RSEM. RSEM creates a temporary
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
766 directory, 'sample_name.temp', into which it puts all intermediate
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
767 output files. If this directory already exists, RSEM overwrites all
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
768 files generated by previous RSEM runs inside of it. By default,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
769 after RSEM finishes, the temporary directory is deleted. Set this
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
770 option to prevent the deletion of this directory and the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
771 intermediate files inside of it. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
772
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
773 --temporary-folder &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
774 Set where to put the temporary files generated by RSEM. If the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
775 folder specified does not exist, RSEM will try to create it.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
776 (Default: sample_name.temp)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
777
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
778 --time
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
779 Output time consumed by each step of RSEM to 'sample_name.time'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
780 (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
781
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
782 PRIOR-ENHANCED RSEM OPTIONS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
783 --run-pRSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
784 Running prior-enhanced RSEM (pRSEM). Prior parameters, i.e.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
785 isoform's initial pseudo-count for RSEM's Gibbs sampling, will be
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
786 learned from input RNA-seq data and an external data set. When pRSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
787 needs and only needs ChIP-seq peak information to partition isoforms
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
788 (e.g. in pRSEM's default partition model), either ChIP-seq peak file
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
789 (with the '--chipseq-peak-file' option) or ChIP-seq FASTQ files for
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
790 target and input and the path for Bowtie executables are required
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
791 (with the '--chipseq-target-read-files &lt;string&gt;',
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
792 '--chipseq-control-read-files &lt;string&gt;', and '--bowtie-path &lt;path&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
793 options), otherwise, ChIP-seq FASTQ files for target and control and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
794 the path to Bowtie executables are required. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
795
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
796 --chipseq-peak-file &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
797 Full path to a ChIP-seq peak file in ENCODE's narrowPeak, i.e.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
798 BED6+4, format. This file is used when running prior-enhanced RSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
799 in the default two-partition model. It partitions isoforms by
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
800 whether they have ChIP-seq overlapping with their transcription
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
801 start site region or not. Each partition will have its own prior
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
802 parameter learned from a training set. This file can be either
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
803 gzipped or ungzipped. (Default: "")
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
804
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
805 --chipseq-target-read-files &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
806 Comma-separated full path of FASTQ read file(s) for ChIP-seq target.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
807 This option is used when running prior-enhanced RSEM. It provides
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
808 information to calculate ChIP-seq peaks and signals. The file(s) can
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
809 be either ungzipped or gzipped with a suffix '.gz' or '.gzip'. The
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
810 options '--bowtie-path &lt;path&gt;' and '--chipseq-control-read-files
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
811 &lt;string&gt;' must be defined when this option is specified. (Default:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
812 "")
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
813
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
814 --chipseq-control-read-files &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
815 Comma-separated full path of FASTQ read file(s) for ChIP-seq conrol.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
816 This option is used when running prior-enhanced RSEM. It provides
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
817 information to call ChIP-seq peaks. The file(s) can be either
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
818 ungzipped or gzipped with a suffix '.gz' or '.gzip'. The options
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
819 '--bowtie-path &lt;path&gt;' and '--chipseq-target-read-files &lt;string&gt;'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
820 must be defined when this option is specified. (Default: "")
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
821
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
822 --chipseq-read-files-multi-targets &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
823 Comma-separated full path of FASTQ read files for multiple ChIP-seq
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
824 targets. This option is used when running prior-enhanced RSEM, where
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
825 prior is learned from multiple complementary data sets. It provides
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
826 information to calculate ChIP-seq signals. All files can be either
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
827 ungzipped or gzipped with a suffix '.gz' or '.gzip'. When this
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
828 option is specified, the option '--bowtie-path &lt;path&gt;' must be
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
829 defined and the option '--partition-model &lt;string&gt;' will be set to
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
830 'cmb_lgt' automatically. (Default: "")
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
831
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
832 --chipseq-bed-files-multi-targets &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
833 Comma-separated full path of BED files for multiple ChIP-seq
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
834 targets. This option is used when running prior-enhanced RSEM, where
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
835 prior is learned from multiple complementary data sets. It provides
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
836 information of ChIP-seq signals and must have at least the first six
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
837 BED columns. All files can be either ungzipped or gzipped with a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
838 suffix '.gz' or '.gzip'. When this option is specified, the option
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
839 '--partition-model &lt;string&gt;' will be set to 'cmb_lgt' automatically.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
840 (Default: "")
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
841
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
842 --cap-stacked-chipseq-reads
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
843 Keep a maximum number of ChIP-seq reads that aligned to the same
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
844 genomic interval. This option is used when running prior-enhanced
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
845 RSEM, where prior is learned from multiple complementary data sets.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
846 This option is only in use when either
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
847 '--chipseq-read-files-multi-targets &lt;string&gt;' or
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
848 '--chipseq-bed-files-multi-targets &lt;string&gt;' is specified. (Default:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
849 off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
850
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
851 --n-max-stacked-chipseq-reads &lt;int&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
852 The maximum number of stacked ChIP-seq reads to keep. This option is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
853 used when running prior-enhanced RSEM, where prior is learned from
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
854 multiple complementary data sets. This option is only in use when
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
855 the option '--cap-stacked-chipseq-reads' is set. (Default: 5)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
856
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
857 --partition-model &lt;string&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
858 A keyword to specify the partition model used by prior-enhanced
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
859 RSEM. It must be one of the following keywords:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
860
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
861 - pk
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
862 Partitioned by whether an isoform has a ChIP-seq peak overlapping
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
863 with its transcription start site (TSS) region. The TSS region is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
864 defined as [TSS-500bp, TSS+500bp]. For simplicity, we refer this
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
865 type of peak as 'TSS peak' when explaining other keywords.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
866
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
867 - pk_lgtnopk
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
868 First partitioned by TSS peak. Then, for isoforms in the 'no TSS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
869 peak' set, a logistic model is employed to further classify them
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
870 into two partitions.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
871
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
872 - lm3, lm4, lm5, or lm6
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
873 Based on their ChIP-seq signals, isoforms are classified into 3,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
874 4, 5, or 6 partitions by a linear regression model.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
875
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
876 - nopk_lm2pk, nopk_lm3pk, nopk_lm4pk, or nopk_lm5pk
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
877 First partitioned by TSS peak. Then, for isoforms in the 'with TSS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
878 peak' set, a linear regression model is employed to further
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
879 classify them into 2, 3, 4, or 5 partitions.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
880
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
881 - pk_lm2nopk, pk_lm3nopk, pk_lm4nopk, or pk_lm5nopk
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
882 First partitioned by TSS peak. Then, for isoforms in the 'no TSS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
883 peak' set, a linear regression model is employed to further
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
884 classify them into 2, 3, 4, or 5 partitions.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
885
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
886 - cmb_lgt
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
887 Using a logistic regression to combine TSS signals from multiple
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
888 complementary data sets and partition training set isoform into
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
889 'expressed' and 'not expressed'. This partition model is only in
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
890 use when either '--chipseq-read-files-multi-targets &lt;string&gt;' or
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
891 '--chipseq-bed-files-multi-targets &lt;string&gt; is specified.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
892
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
893 Parameters for all the above models are learned from a training set.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
894 For detailed explanations, please see prior-enhanced RSEM's paper.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
895 (Default: 'pk')
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
896
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
897 DEPRECATED OPTIONS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
898 The options in this section are deprecated. They are here only for
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
899 compatibility reasons and may be removed in future releases.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
900
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
901 --sam
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
902 Inputs are alignments in SAM format. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
903
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
904 --bam
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
905 Inputs are alignments in BAM format. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
906
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
907 --strand-specific
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
908 Equivalent to '--strandedness forward'. (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
909
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
910 --forward-prob &lt;double&gt;
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
911 Probability of generating a read from the forward strand of a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
912 transcript. Set to 1 for a strand-specific protocol where all
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
913 (upstream) reads are derived from the forward strand, 0 for a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
914 strand-specific protocol where all (upstream) read are derived from
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
915 the reverse strand, or 0.5 for a non-strand-specific protocol.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
916 (Default: off)
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
917
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artbio
parents:
diff changeset
918 DESCRIPTION
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
919 In its default mode, this program aligns input reads against a reference
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
920 transcriptome with Bowtie and calculates expression values using the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
921 alignments. RSEM assumes the data are single-end reads with quality
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
922 scores, unless the '--paired-end' or '--no-qualities' options are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
923 specified. Alternatively, users can use STAR to align reads using the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
924 '--star' option. RSEM has provided options in 'rsem-prepare-reference'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
925 to prepare STAR's genome indices. Users may use an alternative aligner
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
926 by specifying '--alignments', and providing an alignment file in
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
927 SAM/BAM/CRAM format. However, users should make sure that they align
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
928 against the indices generated by 'rsem-prepare-reference' and the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
929 alignment file satisfies the requirements mentioned in ARGUMENTS
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
930 section.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
931
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
932 One simple way to make the alignment file satisfying RSEM's requirements
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
933 is to use the 'convert-sam-for-rsem' script. This script accepts
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
934 SAM/BAM/CRAM files as input and outputs a BAM file. For example, type
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
935 the following command to convert a SAM file, 'input.sam', to a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
936 ready-for-use BAM file, 'input_for_rsem.bam':
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
937
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
938 convert-sam-for-rsem input.sam input_for_rsem
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
939
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
940 For details, please refer to 'convert-sam-for-rsem's documentation page.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
941
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
942 NOTES
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
943 1. Users must run 'rsem-prepare-reference' with the appropriate
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
944 reference before using this program.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
945
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
946 2. For single-end data, it is strongly recommended that the user provide
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
947 the fragment length distribution parameters (--fragment-length-mean and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
948 --fragment-length-sd). For paired-end data, RSEM will automatically
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
949 learn a fragment length distribution from the data.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
950
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
951 3. Some aligner parameters have default values different from their
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
952 original settings.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
953
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
954 4. With the '--calc-pme' option, posterior mean estimates will be
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
955 calculated in addition to maximum likelihood estimates.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
956
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
957 5. With the '--calc-ci' option, 95% credibility intervals and posterior
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
958 mean estimates will be calculated in addition to maximum likelihood
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
959 estimates.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
960
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
961 6. The temporary directory and all intermediate files will be removed
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
962 when RSEM finishes unless '--keep-intermediate-files' is specified.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
963
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
964 With the '--run-pRSEM' option and associated options (see section
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
965 'PRIOR-ENHANCED RSEM OPTIONS' above for details), prior-enhanced RSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
966 will be running. Prior parameters will be learned from supplied external
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
967 data set(s) and assigned as initial pseudo-counts for isoforms in the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
968 corresponding partition for Gibbs sampling.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
969
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
970 OUTPUT
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
971 sample_name.isoforms.results
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
972 File containing isoform level expression estimates. The first line
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
973 contains column names separated by the tab character. The format of
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
974 each line in the rest of this file is:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
975
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
976 transcript_id gene_id length effective_length expected_count TPM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
977 FPKM IsoPct [posterior_mean_count
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
978 posterior_standard_deviation_of_count pme_TPM pme_FPKM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
979 IsoPct_from_pme_TPM TPM_ci_lower_bound TPM_ci_upper_bound
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
980 TPM_coefficient_of_quartile_variation FPKM_ci_lower_bound
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
981 FPKM_ci_upper_bound FPKM_coefficient_of_quartile_variation]
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
982
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
983 Fields are separated by the tab character. Fields within "[]" are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
984 optional. They will not be presented if neither '--calc-pme' nor
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
985 '--calc-ci' is set.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
986
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
987 'transcript_id' is the transcript name of this transcript. 'gene_id'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
988 is the gene name of the gene which this transcript belongs to
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
989 (denote this gene as its parent gene). If no gene information is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
990 provided, 'gene_id' and 'transcript_id' are the same.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
991
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
992 'length' is this transcript's sequence length (poly(A) tail is not
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
993 counted). 'effective_length' counts only the positions that can
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
994 generate a valid fragment. If no poly(A) tail is added,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
995 'effective_length' is equal to transcript length - mean fragment
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
996 length + 1. If one transcript's effective length is less than 1,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
997 this transcript's both effective length and abundance estimates are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
998 set to 0.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
999
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1000 'expected_count' is the sum of the posterior probability of each
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1001 read comes from this transcript over all reads. Because 1) each read
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1002 aligning to this transcript has a probability of being generated
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1003 from background noise; 2) RSEM may filter some alignable low quality
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1004 reads, the sum of expected counts for all transcript are generally
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1005 less than the total number of reads aligned.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1006
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1007 'TPM' stands for Transcripts Per Million. It is a relative measure
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1008 of transcript abundance. The sum of all transcripts' TPM is 1
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1009 million. 'FPKM' stands for Fragments Per Kilobase of transcript per
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1010 Million mapped reads. It is another relative measure of transcript
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1011 abundance. If we define l_bar be the mean transcript length in a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1012 sample, which can be calculated as
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1013
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1014 l_bar = \sum_i TPM_i / 10^6 * effective_length_i (i goes through
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1015 every transcript),
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1016
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1017 the following equation is hold:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1018
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1019 FPKM_i = 10^3 / l_bar * TPM_i.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1020
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1021 We can see that the sum of FPKM is not a constant across samples.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1022
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1023 'IsoPct' stands for isoform percentage. It is the percentage of this
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1024 transcript's abandunce over its parent gene's abandunce. If its
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1025 parent gene has only one isoform or the gene information is not
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1026 provided, this field will be set to 100.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1027
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1028 'posterior_mean_count', 'pme_TPM', 'pme_FPKM' are posterior mean
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1029 estimates calculated by RSEM's Gibbs sampler.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1030 'posterior_standard_deviation_of_count' is the posterior standard
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1031 deviation of counts. 'IsoPct_from_pme_TPM' is the isoform percentage
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1032 calculated from 'pme_TPM' values.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1033
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1034 'TPM_ci_lower_bound', 'TPM_ci_upper_bound', 'FPKM_ci_lower_bound'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1035 and 'FPKM_ci_upper_bound' are lower(l) and upper(u) bounds of 95%
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1036 credibility intervals for TPM and FPKM values. The bounds are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1037 inclusive (i.e. [l, u]).
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1038
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1039 'TPM_coefficient_of_quartile_variation' and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1040 'FPKM_coefficient_of_quartile_variation' are coefficients of
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1041 quartile variation (CQV) for TPM and FPKM values. CQV is a robust
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1042 way of measuring the ratio between the standard deviation and the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1043 mean. It is defined as
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1044
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1045 CQV := (Q3 - Q1) / (Q3 + Q1),
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1046
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1047 where Q1 and Q3 are the first and third quartiles.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1048
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1049 sample_name.genes.results
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1050 File containing gene level expression estimates. The first line
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1051 contains column names separated by the tab character. The format of
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1052 each line in the rest of this file is:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1053
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1054 gene_id transcript_id(s) length effective_length expected_count TPM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1055 FPKM [posterior_mean_count posterior_standard_deviation_of_count
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1056 pme_TPM pme_FPKM TPM_ci_lower_bound TPM_ci_upper_bound
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1057 TPM_coefficient_of_quartile_variation FPKM_ci_lower_bound
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1058 FPKM_ci_upper_bound FPKM_coefficient_of_quartile_variation]
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1059
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1060 Fields are separated by the tab character. Fields within "[]" are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1061 optional. They will not be presented if neither '--calc-pme' nor
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1062 '--calc-ci' is set.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1063
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1064 'transcript_id(s)' is a comma-separated list of transcript_ids
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1065 belonging to this gene. If no gene information is provided,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1066 'gene_id' and 'transcript_id(s)' are identical (the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1067 'transcript_id').
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1068
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1069 A gene's 'length' and 'effective_length' are defined as the weighted
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1070 average of its transcripts' lengths and effective lengths (weighted
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1071 by 'IsoPct'). A gene's abundance estimates are just the sum of its
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1072 transcripts' abundance estimates.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1073
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1074 sample_name.alleles.results
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1075 Only generated when the RSEM references are built with
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1076 allele-specific transcripts.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1077
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1078 This file contains allele level expression estimates for
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1079 allele-specific expression calculation. The first line contains
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1080 column names separated by the tab character. The format of each line
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1081 in the rest of this file is:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1082
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1083 allele_id transcript_id gene_id length effective_length
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1084 expected_count TPM FPKM AlleleIsoPct AlleleGenePct
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1085 [posterior_mean_count posterior_standard_deviation_of_count pme_TPM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1086 pme_FPKM AlleleIsoPct_from_pme_TPM AlleleGenePct_from_pme_TPM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1087 TPM_ci_lower_bound TPM_ci_upper_bound
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1088 TPM_coefficient_of_quartile_variation FPKM_ci_lower_bound
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1089 FPKM_ci_upper_bound FPKM_coefficient_of_quartile_variation]
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1090
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1091 Fields are separated by the tab character. Fields within "[]" are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1092 optional. They will not be presented if neither '--calc-pme' nor
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1093 '--calc-ci' is set.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1094
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1095 'allele_id' is the allele-specific name of this allele-specific
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1096 transcript.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1097
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1098 'AlleleIsoPct' stands for allele-specific percentage on isoform
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1099 level. It is the percentage of this allele-specific transcript's
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1100 abundance over its parent transcript's abundance. If its parent
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1101 transcript has only one allele variant form, this field will be set
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1102 to 100.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1103
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1104 'AlleleGenePct' stands for allele-specific percentage on gene level.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1105 It is the percentage of this allele-specific transcript's abundance
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1106 over its parent gene's abundance.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1107
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1108 'AlleleIsoPct_from_pme_TPM' and 'AlleleGenePct_from_pme_TPM' have
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1109 similar meanings. They are calculated based on posterior mean
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1110 estimates.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1111
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1112 Please note that if this file is present, the fields 'length' and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1113 'effective_length' in 'sample_name.isoforms.results' should be
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1114 interpreted similarly as the corresponding definitions in
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1115 'sample_name.genes.results'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1116
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1117 sample_name.transcript.bam
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1118 Only generated when --no-bam-output is not specified.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1119
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1120 'sample_name.transcript.bam' is a BAM-formatted file of read
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1121 alignments in transcript coordinates. The MAPQ field of each
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1122 alignment is set to min(100, floor(-10 * log10(1.0 - w) + 0.5)),
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1123 where w is the posterior probability of that alignment being the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1124 true mapping of a read. In addition, RSEM pads a new tag ZW:f:value,
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1125 where value is a single precision floating number representing the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1126 posterior probability. Because this file contains all alignment
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1127 lines produced by bowtie or user-specified aligners, it can also be
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1128 used as a replacement of the aligner generated BAM/SAM file.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1129
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1130 sample_name.transcript.sorted.bam and sample_name.transcript.sorted.bam.bai
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1131 Only generated when --no-bam-output is not specified and --sort-bam-by-coordinate is specified.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1132
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1133 'sample_name.transcript.sorted.bam' and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1134 'sample_name.transcript.sorted.bam.bai' are the sorted BAM file and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1135 indices generated by samtools (included in RSEM package).
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1136
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1137 sample_name.genome.bam
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1138 Only generated when --no-bam-output is not specified and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1139 --output-genome-bam is specified.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1140
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1141 'sample_name.genome.bam' is a BAM-formatted file of read alignments
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1142 in genomic coordinates. Alignments of reads that have identical
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1143 genomic coordinates (i.e., alignments to different isoforms that
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1144 share the same genomic region) are collapsed into one alignment. The
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1145 MAPQ field of each alignment is set to min(100, floor(-10 *
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1146 log10(1.0 - w) + 0.5)), where w is the posterior probability of that
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1147 alignment being the true mapping of a read. In addition, RSEM pads a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1148 new tag ZW:f:value, where value is a single precision floating
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1149 number representing the posterior probability. If an alignment is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1150 spliced, a XS:A:value tag is also added, where value is either '+'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1151 or '-' indicating the strand of the transcript it aligns to.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1152
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1153 sample_name.genome.sorted.bam and sample_name.genome.sorted.bam.bai
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1154 Only generated when --no-bam-output is not specified, and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1155 --sort-bam-by-coordinate and --output-genome-bam are specified.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1156
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1157 'sample_name.genome.sorted.bam' and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1158 'sample_name.genome.sorted.bam.bai' are the sorted BAM file and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1159 indices generated by samtools (included in RSEM package).
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1160
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1161 sample_name.time
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1162 Only generated when --time is specified.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1163
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1164 It contains time (in seconds) consumed by aligning reads, estimating
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1165 expression levels and calculating credibility intervals.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1166
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1167 sample_name.stat
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1168 This is a folder instead of a file. All model related statistics are
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1169 stored in this folder. Use 'rsem-plot-model' can generate plots
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1170 using this folder.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1171
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1172 'sample_name.stat/sample_name.cnt' contains alignment statistics.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1173 The format and meanings of each field are described in
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1174 'cnt_file_description.txt' under RSEM directory.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1175
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1176 'sample_name.stat/sample_name.model' stores RNA-Seq model parameters
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1177 learned from the data. The format and meanings of each filed of this
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1178 file are described in 'model_file_description.txt' under RSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1179 directory.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1180
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1181 The following four output files will be generated only by
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1182 prior-enhanced RSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1183
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1184 - 'sample_name.stat/sample_name_prsem.all_tr_features'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1185 It stores isofrom features for deriving and assigning pRSEM prior.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1186 The first line is a header and the rest is one isoform per line.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1187 The description for each column is:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1188
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1189 * trid: transcript ID from input annotation
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1190
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1191 * geneid: gene ID from input anntation
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1192
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1193 * chrom: isoform's chromosome name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1194
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1195 * strand: isoform's strand name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1196
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1197 * start: isoform's end with the lowest genomic loci
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1198
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1199 * end: isoform's end with the highest genomic loci
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1200
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1201 * tss_mpp: average mappability of [TSS-500bp, TSS+500bp], where
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1202 TSS is isoform's transcription start site, i.e. 5'-end
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1203
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1204 * body_mpp: average mappability of (TSS+500bp, TES-500bp), where
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1205 TES is isoform's transcription end site, i.e. 3'-end
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1206
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1207 * tes_mpp: average mappability of [TES-500bp, TES+500bp]
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1208
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1209 * pme_count: isoform's fragment or read count from RSEM's
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1210 posterior mean estimates
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1211
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1212 * tss: isoform's TSS loci
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1213
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1214 * tss_pk: equal to 1 if isoform's [TSS-500bp, TSS+500bp] region
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1215 overlaps with a RNA Pol II peak; 0 otherwise
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1216
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1217 * is_training: equal to 1 if isoform is in the training set where
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1218 Pol II prior is learned; 0 otherwise
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1219
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1220 - 'sample_name.stat/sample_name_prsem.all_tr_prior'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1221 It stores prior parameters for every isoform. This file does not
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1222 have a header. Each line contains a prior parameter and an
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1223 isoform's transcript ID delimited by " # ".
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1224
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1225 - 'sample_name.stat/sample_name_uniform_prior_1.isoforms.results'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1226 RSEM's posterior mean estimates on the isoform level with an
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1227 initial pseudo-count of one for every isoform. It is in the same
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1228 format as the 'sample_name.isoforms.results'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1229
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1230 - 'sample_name.stat/sample_name_uniform_prior_1.genes.results'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1231 RSEM's posterior mean estimates on the gene level with an initial
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1232 pseudo-count of one for every isoform. It is in the same format as
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1233 the 'sample_name.genes.results'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1234
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1235 When learning prior from multiple external data sets in
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1236 prior-enhanced RSEM, two additional output files will be generated.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1237
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1238 - 'sample_name.stat/sample_name.pval_LL'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1239 It stores a p-value and a log-likelihood. The p-value indicates
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1240 whether the combination of multiple complementary data sets is
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1241 informative for RNA-seq quantification. The log-likelihood shows
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1242 how well pRSEM's Dirichlet-multinomial model fits the read counts
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1243 of partitioned training set isoforms.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1244
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1245 - 'sample_name.stat/sample_name.lgt_mdl.RData'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1246 It stores an R object named 'glmmdl', which is a logistic
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1247 regression model on the training set isoforms and multiple
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1248 external data sets.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1249
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1250 In addition, extra columns will be added to
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1251 'sample_name.stat/all_tr_features'
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1252
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1253 * is_expr: equal to 1 if isoform has an abundance &gt;= 1 TPM and a
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1254 non-zero read count from RSEM's posterior mean estimates; 0
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1255 otherwise
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1256
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1257 * "$external_data_set_basename": log10 of external data's signal at
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1258 [TSS-500, TSS+500]. Signal is the number of reads aligned within
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1259 that interval and normalized to RPKM by read depth and interval
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1260 length. It will be set to -4 if no read aligned to that interval.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1261
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1262 There are multiple columns like this one, where each represents an
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1263 external data set.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1264
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1265 * prd_expr_prob: predicted probability from logistic regression
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1266 model on whether this isoform is expressed or not. A probability
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1267 higher than 0.5 is considered as expressed
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1268
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1269 * partition: group index, to which this isoforms is partitioned
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1270
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1271 * prior: prior parameter for this isoform
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1272
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1273 EXAMPLES
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1274 Assume the path to the bowtie executables is in the user's PATH
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1275 environment variable. Reference files are under '/ref' with name
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1276 'mouse_125'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1277
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1278 1) '/data/mmliver.fq', single-end reads with quality scores. Quality
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1279 scores are encoded as for 'GA pipeline version &gt;= 1.3'. We want to use 8
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1280 threads and generate a genome BAM file. In addition, we want to append
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1281 gene/transcript names to the result files:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1282
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1283 rsem-calculate-expression --phred64-quals \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1284 -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1285 --append-names \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1286 --output-genome-bam \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1287 /data/mmliver.fq \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1288 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1289 mmliver_single_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1290
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1291 2) '/data/mmliver_1.fq' and '/data/mmliver_2.fq', stranded paired-end
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1292 reads with quality scores. Suppose the library is prepared using TruSeq
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1293 Stranded Kit, which means the first mate should map to the reverse
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1294 strand. Quality scores are in SANGER format. We want to use 8 threads
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1295 and do not generate a genome BAM file:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1296
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1297 rsem-calculate-expression -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1298 --paired-end \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1299 --strandedness reverse \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1300 /data/mmliver_1.fq \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1301 /data/mmliver_2.fq \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1302 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1303 mmliver_paired_end_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1304
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1305 3) '/data/mmliver.fa', single-end reads without quality scores. We want
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1306 to use 8 threads:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1307
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1308 rsem-calculate-expression -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1309 --no-qualities \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1310 /data/mmliver.fa \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1311 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1312 mmliver_single_without_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1313
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1314 4) Data are the same as 1). This time we assume the bowtie executables
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1315 are under '/sw/bowtie'. We want to take a fragment length distribution
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1316 into consideration. We set the fragment length mean to 150 and the
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1317 standard deviation to 35. In addition to a BAM file, we also want to
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1318 generate credibility intervals. We allow RSEM to use 1GB of memory for
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1319 CI calculation:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1320
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1321 rsem-calculate-expression --bowtie-path /sw/bowtie \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1322 --phred64-quals \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1323 --fragment-length-mean 150.0 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1324 --fragment-length-sd 35.0 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1325 -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1326 --output-genome-bam \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1327 --calc-ci \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1328 --ci-memory 1024 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1329 /data/mmliver.fq \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1330 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1331 mmliver_single_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1332
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1333 5) '/data/mmliver_paired_end_quals.bam', BAM-formatted alignments for
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1334 paired-end reads with quality scores. We want to use 8 threads:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1335
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1336 rsem-calculate-expression --paired-end \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1337 --alignments \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1338 -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1339 /data/mmliver_paired_end_quals.bam \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1340 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1341 mmliver_paired_end_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1342
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1343 6) '/data/mmliver_1.fq.gz' and '/data/mmliver_2.fq.gz', paired-end reads
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1344 with quality scores and read files are compressed by gzip. We want to
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1345 use STAR to aligned reads and assume STAR executable is '/sw/STAR'.
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1346 Suppose we want to use 8 threads and do not generate a genome BAM file:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1347
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1348 rsem-calculate-expression --paired-end \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1349 --star \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1350 --star-path /sw/STAR \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1351 --gzipped-read-file \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1352 --paired-end \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1353 -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1354 /data/mmliver_1.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1355 /data/mmliver_2.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1356 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1357 mmliver_paired_end_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1358
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1359 7) In the above example, suppose we want to run prior-enhanced RSEM
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1360 instead. Assuming we want to learn priors from a ChIP-seq peak file
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1361 '/data/mmlive.narrowPeak.gz':
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1362
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1363 rsem-calculate-expression --star \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1364 --star-path /sw/STAR \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1365 --gzipped-read-file \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1366 --paired-end \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1367 --calc-pme \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1368 --run-pRSEM \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1369 --chipseq-peak-file /data/mmliver.narrowPeak.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1370 -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1371 /data/mmliver_1.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1372 /data/mmliver_2.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1373 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1374 mmliver_paired_end_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1375
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1376 8) Similar to the example in 7), suppose we want to use the partition
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1377 model 'pk_lm2nopk' (partitioning isoforms by Pol II TSS peak first and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1378 then partitioning 'no TSS peak' isoforms into two bins by a linear
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1379 regression model), and we want to partition isoforms by RNA Pol II's
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1380 ChIP-seq read files '/data/mmliver_PolIIRep1.fq.gz' and
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1381 '/data/mmliver_PolIIRep2.fq.gz', and the control ChIP-seq read files
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1382 '/data/mmliver_ChIPseqCtrl.fq.gz'. Also, assuming Bowtie's executables
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1383 are under '/sw/bowtie/':
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1384
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1385 rsem-calculate-expression --star \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1386 --star-path /sw/STAR \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1387 --gzipped-read-file \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1388 --paired-end \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1389 --calc-pme \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1390 --run-pRSEM \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1391 --chipseq-target-read-files /data/mmliver_PolIIRep1.fq.gz,/data/mmliver_PolIIRep2.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1392 --chipseq-control-read-files /data/mmliver_ChIPseqCtrl.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1393 --partition-model pk_lm2nopk \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1394 --bowtie-path /sw/bowtie \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1395 -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1396 /data/mmliver_1.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1397 /data/mmliver_2.fq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1398 /ref/mouse_125 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1399 mmliver_paired_end_quals
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1400
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1401 9) Similar to the example in 8), suppose we want to derive prior from
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1402 four histone modification ChIP-seq read data sets:
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1403 '/data/H3K27Ac.fastq.gz', '/data/H3K4me1.fastq.gz',
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1404 '/data/H3K4me2.fastq.gz', and '/data/H3K4me3.fastq.gz'. Also, assuming
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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diff changeset
1405 Bowtie's executables are under '/sw/bowtie/':
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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diff changeset
1406
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1407 rsem-calculate-expression --star \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1408 --star-path /sw/STAR \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1409 --gzipped-read-file \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1410 --paired-end \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1411 --calc-pme \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1412 --run-pRSEM \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
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diff changeset
1413 --partition-model cmb_lgt \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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diff changeset
1414 --chipseq-read-files-multi-targets /data/H3K27Ac.fastq.gz,/data/H3K4me1.fastq.gz,/data/H3K4me2.fastq.gz,/data/H3K4me3.fastq.gz \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
artbio
parents:
diff changeset
1415 --bowtie-path /sw/bowtie \
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parents:
diff changeset
1416 -p 8 \
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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diff changeset
1417 /data/mmliver_1.fq.gz \
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diff changeset
1418 /data/mmliver_2.fq.gz \
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diff changeset
1419 /ref/mouse_125 \
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diff changeset
1420 mmliver_paired_end_quals
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diff changeset
1421
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1422 </help>
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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1423 <citations>
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diff changeset
1424 <citation type="doi">10.1186/1471-2105-12-323</citation>
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diff changeset
1425 </citations>
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1426
e5e836936d60 planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit d84a0359354698a4b29df12ab581c2618bffcf80
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1427 </tool>