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author | artbio |
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date | Sun, 15 Dec 2024 16:37:13 +0000 |
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<tool id="wisecondorx_predict" name="WisecondorX predict" version="@WRAPPER_VERSION@" profile="@PROFILE@"> <description> CNVs </description> <macros> <import>macro.xml</import> </macros> <expand macro="requirements"/> <stdio> <exit_code range="1:" level="fatal" description="Error occured" /> </stdio> <command detect_errors="exit_code"><![CDATA[ @pipefail@ ln -s $npz_input sample.npz && ln -s $reference reference.npz && WisecondorX predict sample.npz reference.npz sample --plot --bed ]]></command> <inputs> <param name="npz_input" type="data" format="npz" label="npz file from sample to analyse"/> <param name="reference" type="data" format="npz" label="npz reference built with WisecondorX build"/> </inputs> <outputs> <data name="aberrations" format="bed" label="sample abberations" from_work_dir="sample_aberrations.bed"/> <data name="bins" format="bed" label="sample bins" from_work_dir="sample_bins.bed"/> <data name="segments" format="bed" label="sample segments" from_work_dir="sample_segments.bed"/> <data name="statistics" format="txt" label="statistics" from_work_dir="sample_statistics.txt"/> <collection name="plots" type="list" format="png" label="CNV plots"> <discover_datasets pattern="__name_and_ext__" directory="sample.plots" /> </collection> </outputs> <tests> <test expect_num_outputs="5"> <param name="npz_input" value="input_sample_predict.npz" ftype="npz" /> <param name="reference" value="input_reference_predict.10000kb.npz" /> <output name="aberrations" ftype="bed" file="predict_abberations.bed" /> <output name="bins" ftype="bed" file="predict_bins.bed" compare="sim_size" delta="1000"/> <output name="segments" ftype="bed" file="predict_segments.bed"/> <output name="statistics" ftype="txt" file="predict_statistics.txt" compare="sim_size" delta="1000"/> <output_collection name="plots" type="list"> <element name="chr1" file="chr1.png" compare="sim_size" delta="10000"/> <element name="chr10" file="chr10.png" compare="sim_size" delta="10000"/> <element name="chr11" file="chr11.png" compare="sim_size" delta="10000"/> <element name="chr12" file="chr12.png" compare="sim_size" delta="10000"/> <element name="chr13" file="chr13.png" compare="sim_size" delta="10000"/> <element name="chr14" file="chr14.png" compare="sim_size" delta="10000"/> <element name="chr15" file="chr15.png" compare="sim_size" delta="10000"/> <element name="chr16" file="chr16.png" compare="sim_size" delta="10000"/> <element name="chr17" file="chr17.png" compare="sim_size" delta="10000"/> <element name="chr18" file="chr18.png" compare="sim_size" delta="10000"/> <element name="chr19" file="chr19.png" compare="sim_size" delta="10000"/> <element name="chr2" file="chr2.png" compare="sim_size" delta="10000"/> <element name="chr20" file="chr20.png" compare="sim_size" delta="10000"/> <element name="chr21" file="chr21.png" compare="sim_size" delta="10000"/> <element name="chr22" file="chr22.png" compare="sim_size" delta="10000"/> <element name="chr3" file="chr3.png" compare="sim_size" delta="10000"/> <element name="chr4" file="chr4.png" compare="sim_size" delta="10000"/> <element name="chr5" file="chr5.png" compare="sim_size" delta="10000"/> <element name="chr6" file="chr6.png" compare="sim_size" delta="10000"/> <element name="chr7" file="chr7.png" compare="sim_size" delta="10000"/> <element name="chr8" file="chr8.png" compare="sim_size" delta="10000"/> <element name="chr9" file="chr9.png" compare="sim_size" delta="10000"/> <element name="chrX" file="chrX.png" compare="sim_size" delta="10000"/> <element name="genome_wide" file="genome_wide.png" compare="sim_size" delta="10000"/> </output_collection> </test> </tests> <help> @help@ <![CDATA[ .. class:: infomark **WisecondorX predict test_input.npz reference_input.npz output_id [--optional arguments]** Option List:: --minrefbins Minimum amount of sensible reference bins per target bin; should generally not be tweaked (default: x=150) --maskrepeats Bins with distances > mean + sd * 3 in the reference will be masked. This parameter represents the number of masking cycles and defines the stringency of the blacklist (default: x=5) **Should be a multiple of the 5e3**. Not exposed in this Galaxy wrapper. --zscore Z-score cutoff to call segments as aberrations (default: x=5) --alpha P-value cutoff for calling circular binary segmentation breakpoints (default: x=1e-4). Not exposed in this Galaxy wrapper. --beta When beta is given, --zscore is ignored. Beta sets a ratio cutoff for aberration calling. It's a number between 0 (liberal) and 1 (conservative) and, when used, is optimally close to the purity (e.g. fetal/tumor fraction) Not exposed in this Galaxy wrapper. --blacklist Blacklist for masking additional regions; requires headerless .bed file. This is particularly useful when the reference set is too small to recognize some obvious loci (such as centromeres). Not exposed in this Galaxy wrapper. --gender Force WisecondorX to analyze this case as male (M) or female (F). Useful when e.g. dealing with a loss of chromosome Y, which causes erroneous gender predictions (choices: x=F or x=M). Not exposed in this Galaxy wrapper. --bed Outputs tab-delimited .bed files. --plot Outputs custom .png plots, directly interpretable. --ylim [a,b] Force WisecondorX to use y-axis interval [a,b] during plotting, e.g. [-2,2]. Not exposed in this Galaxy wrapper. --cairo Some operating systems require the cairo bitmap type to write plots. Not exposed in this Galaxy wrapper. --seed Random seed for segmentation algorithm (default:None). Not exposed in this Galaxy wrapper. ]]></help> <citations> <citation type="doi">10.1093/nar/gky1263</citation> </citations> </tool>