# HG changeset patch # User bgruening # Date 1594674894 14400 # Node ID 53d74155bb52dffd10d34b853f24613bd3a3d26e # Parent e572598580c217612d996c4982858e954813f6ed "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4c71464b5f5047e0745067c115c37a5d06867649" diff -r e572598580c2 -r 53d74155bb52 macros.xml --- a/macros.xml Sat Apr 25 11:56:32 2020 -0400 +++ b/macros.xml Mon Jul 13 17:14:54 2020 -0400 @@ -1,15 +1,15 @@ - 0.14.1.2 + 1.3.0 galaxy0 - q6 + q7 + salmon seqtk - salmon samtools vpolo - pandas - scipy + pandas + scipy @@ -358,7 +358,7 @@ #end if salmon quant - --index $index_path + --index '$index_path' #if $quant_type.input.single_or_paired.single_or_paired_opts == 'single': --libType ${quant_type.input.single_or_paired.strandedness} #if $compressed == 'GZ': diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/complete_ref_lens.bin Binary file test-data/cached_locally/prebuilt_index/complete_ref_lens.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/ctable.bin Binary file test-data/cached_locally/prebuilt_index/ctable.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/ctg_offsets.bin Binary file test-data/cached_locally/prebuilt_index/ctg_offsets.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/duplicate_clusters.tsv --- a/test-data/cached_locally/prebuilt_index/duplicate_clusters.tsv Sat Apr 25 11:56:32 2020 -0400 +++ b/test-data/cached_locally/prebuilt_index/duplicate_clusters.tsv Mon Jul 13 17:14:54 2020 -0400 @@ -1,1 +1,1 @@ -RetainedTxp DuplicateTxp +RetainedRef DuplicateRef diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/hash.bin Binary file test-data/cached_locally/prebuilt_index/hash.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/header.json --- a/test-data/cached_locally/prebuilt_index/header.json Sat Apr 25 11:56:32 2020 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,14 +0,0 @@ -{ - "value0": { - "IndexType": 1, - "IndexVersion": "q6", - "UsesKmers": true, - "KmerLen": 31, - "BigSA": false, - "PerfectHash": false, - "SeqHash": "238a71484cdcf251805629c612f7edd515eb1a23d9be2e2014b62fd5931eb4ac", - "NameHash": "2fc64c34c65a78d18fec933772e1aa454cfed52b2b48d43eb75d2933a22feb04", - "SeqHash512": "a45ea3d0c5f11c16a45b8b75f902f908996117d7486d72b3d19a8ae619b07b22f3ca0888f5ee6e9dbabf639ae51f7fc74b8eea8808435bbb2ec70b96162849aa", - "NameHash512": "6123f1c58bf2153fca204e97fa72a2d25460242f14b886e421316ff251cc57293e77c02d536122e97f46d2508c626e42a35a1798209766359904ddf49fb7bf33" - } -} \ No newline at end of file diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/indexing.log --- a/test-data/cached_locally/prebuilt_index/indexing.log Sat Apr 25 11:56:32 2020 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -[2019-09-08 20:35:10.408] [jLog] [info] building index -[2019-09-08 20:35:10.448] [jLog] [info] done building index diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/info.json --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cached_locally/prebuilt_index/info.json Mon Jul 13 17:14:54 2020 -0400 @@ -0,0 +1,22 @@ +{ + "index_version": 4, + "reference_gfa": [ + "foo" + ], + "sampling_type": "dense", + "k": 31, + "num_kmers": 18902, + "num_contigs": 23, + "seq_length": 19592, + "have_ref_seq": true, + "have_edge_vec": false, + "SeqHash": "238a71484cdcf251805629c612f7edd515eb1a23d9be2e2014b62fd5931eb4ac", + "NameHash": "2fc64c34c65a78d18fec933772e1aa454cfed52b2b48d43eb75d2933a22feb04", + "SeqHash512": "a45ea3d0c5f11c16a45b8b75f902f908996117d7486d72b3d19a8ae619b07b22f3ca0888f5ee6e9dbabf639ae51f7fc74b8eea8808435bbb2ec70b96162849aa", + "NameHash512": "6123f1c58bf2153fca204e97fa72a2d25460242f14b886e421316ff251cc57293e77c02d536122e97f46d2508c626e42a35a1798209766359904ddf49fb7bf33", + "DecoySeqHash": "e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855", + "DecoyNameHash": "e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855", + "num_decoys": 0, + "first_decoy_index": 18446744073709551615, + "keep_duplicates": false +} \ No newline at end of file diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/mphf.bin Binary file test-data/cached_locally/prebuilt_index/mphf.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/pos.bin Binary file test-data/cached_locally/prebuilt_index/pos.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/pre_indexing.log --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cached_locally/prebuilt_index/pre_indexing.log Mon Jul 13 17:14:54 2020 -0400 @@ -0,0 +1,3 @@ +[2020-07-09 14:16:39.483] [jLog] [warning] The salmon index is being built without any decoy sequences. It is recommended that decoy sequence (either computed auxiliary decoy sequence or the genome of the organism) be provided during indexing. Further details can be found at https://salmon.readthedocs.io/en/latest/salmon.html#preparing-transcriptome-indices-mapping-based-mode. +[2020-07-09 14:16:39.484] [jLog] [info] building index +[2020-07-09 14:16:39.619] [jLog] [info] done building index diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/quasi_index.log --- a/test-data/cached_locally/prebuilt_index/quasi_index.log Sat Apr 25 11:56:32 2020 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,12 +0,0 @@ -[2019-09-08 20:35:10.409] [jointLog] [info] [Step 1 of 4] : counting k-mers -[2019-09-08 20:35:10.412] [jointLog] [info] Replaced 0 non-ATCG nucleotides -[2019-09-08 20:35:10.412] [jointLog] [info] Clipped poly-A tails from 0 transcripts -[2019-09-08 20:35:10.412] [jointLog] [info] Building rank-select dictionary and saving to disk -[2019-09-08 20:35:10.412] [jointLog] [info] done -[2019-09-08 20:35:10.412] [jointLog] [info] Writing sequence data to file . . . -[2019-09-08 20:35:10.412] [jointLog] [info] done -[2019-09-08 20:35:10.412] [jointLog] [info] Building 32-bit suffix array (length of generalized text is 28,577) -[2019-09-08 20:35:10.413] [jointLog] [info] Building suffix array . . . -[2019-09-08 20:35:10.445] [jointLog] [info] khash had 18,902 keys -[2019-09-08 20:35:10.445] [jointLog] [info] saving hash to disk . . . -[2019-09-08 20:35:10.447] [jointLog] [info] done diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/rank.bin Binary file test-data/cached_locally/prebuilt_index/rank.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/refAccumLengths.bin Binary file test-data/cached_locally/prebuilt_index/refAccumLengths.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/refInfo.json --- a/test-data/cached_locally/prebuilt_index/refInfo.json Sat Apr 25 11:56:32 2020 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,5 +0,0 @@ -{ - "ReferenceFiles": [ - "test-data/transcripts.fasta" - ] -} \ No newline at end of file diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/ref_indexing.log --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cached_locally/prebuilt_index/ref_indexing.log Mon Jul 13 17:14:54 2020 -0400 @@ -0,0 +1,29 @@ +[2020-07-09 14:16:39.484] [puff::index::jointLog] [info] Running fixFasta +[2020-07-09 14:16:39.490] [puff::index::jointLog] [info] Replaced 0 non-ATCG nucleotides +[2020-07-09 14:16:39.490] [puff::index::jointLog] [info] Clipped poly-A tails from 0 transcripts +[2020-07-09 14:16:39.491] [puff::index::jointLog] [info] Filter size not provided; estimating from number of distinct k-mers +[2020-07-09 14:16:39.494] [puff::index::jointLog] [info] ntHll estimated 54811 distinct k-mers, setting filter size to 2^20 +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Starting the Pufferfish indexing by reading the GFA binary file. +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Setting the index/BinaryGfa directory foo +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Done wrapping the rank vector with a rank9sel structure. +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] contig count for validation: 23 +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Total # of Contigs : 23 +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Total # of numerical Contigs : 23 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] Total # of contig vec entries: 36 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] bits per offset entry 6 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] Done constructing the contig vector. 24 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] # segments = 23 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] total length = 19,592 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] Reading the reference files ... +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] positional integer width = 15 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] seqSize = 19,592 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] rankSize = 19,592 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] edgeVecSize = 0 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] num keys = 18,902 +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] mphf size = 0.0125198 MB +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] chunk size = 9,796 +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] chunk 0 = [0, 9,796) +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] chunk 1 = [9,796, 19,562) +[2020-07-09 14:16:39.618] [puff::index::jointLog] [info] finished populating pos vector +[2020-07-09 14:16:39.618] [puff::index::jointLog] [info] writing index components +[2020-07-09 14:16:39.619] [puff::index::jointLog] [info] finished writing dense pufferfish index diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/reflengths.bin Binary file test-data/cached_locally/prebuilt_index/reflengths.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/refseq.bin Binary file test-data/cached_locally/prebuilt_index/refseq.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/rsd.bin Binary file test-data/cached_locally/prebuilt_index/rsd.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/sa.bin Binary file test-data/cached_locally/prebuilt_index/sa.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/seq.bin Binary file test-data/cached_locally/prebuilt_index/seq.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/txpInfo.bin Binary file test-data/cached_locally/prebuilt_index/txpInfo.bin has changed diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/prebuilt_index/versionInfo.json --- a/test-data/cached_locally/prebuilt_index/versionInfo.json Sat Apr 25 11:56:32 2020 -0400 +++ b/test-data/cached_locally/prebuilt_index/versionInfo.json Mon Jul 13 17:14:54 2020 -0400 @@ -1,6 +1,7 @@ { - "indexVersion": 4, + "indexVersion": 5, "hasAuxIndex": false, "auxKmerLength": 31, - "indexType": 1 + "indexType": 2, + "salmonVersion": "1.3.0" } \ No newline at end of file diff -r e572598580c2 -r 53d74155bb52 test-data/cached_locally/salmon_indexes_versioned.loc --- a/test-data/cached_locally/salmon_indexes_versioned.loc Sat Apr 25 11:56:32 2020 -0400 +++ b/test-data/cached_locally/salmon_indexes_versioned.loc Mon Jul 13 17:14:54 2020 -0400 @@ -30,4 +30,4 @@ #dm3 dm3 D. melanogaster (dm3) /depot/data2/galaxy/salmon_indexes/dm3/version_1 q5 # # -hg19_transcript_subset hg19 Human (Homo sapiens): hg19 transcript test subset ${__HERE__}/prebuilt_index q6 +hg19_transcript_subset hg19 Human (Homo sapiens): hg19 transcript test subset ${__HERE__}/prebuilt_index q7