annotate test-data/mapping_report_pbat.txt @ 18:bb74e17e47d7 draft

"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit 02087ce2966cf8b4aac9197a41171e7f986c11d1"
author bgruening
date Tue, 01 Oct 2019 12:43:53 -0400
parents aa9bf0f29a9f
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1 Bismark report for: input_1.fq (version: v0.22.1)
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2 Option '--pbat' specified: alignments to original strands (OT and OB) strands were ignored (i.e. not performed)
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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3 Bismark was run with Bowtie 2 against the bisulfite genome of /tmp/tmpuqE7r1/ with the specified options: -q -L 20 -D 15 -R 2 --score-min L,0,-0.2 --ignore-quals --quiet
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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5 Final Alignment report
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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6 ======================
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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7 Sequences analysed in total: 44115
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8 Number of alignments with a unique best hit from the different alignments: 13
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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9 Mapping efficiency: 0.0%
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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10 Sequences with no alignments under any condition: 44059
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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11 Sequences did not map uniquely: 43
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12 Sequences which were discarded because genomic sequence could not be extracted: 0
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13
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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14 Number of sequences with unique best (first) alignment came from the bowtie output:
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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15 CT/CT: 0 ((converted) top strand)
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16 CT/GA: 0 ((converted) bottom strand)
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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17 GA/CT: 11 (complementary to (converted) top strand)
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18 GA/GA: 2 (complementary to (converted) bottom strand)
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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20 Final Cytosine Methylation Report
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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21 =================================
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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22 Total number of C's analysed: 307
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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24 Total methylated C's in CpG context: 1
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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25 Total methylated C's in CHG context: 3
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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26 Total methylated C's in CHH context: 227
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27 Total methylated C's in Unknown context: 0
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29 Total unmethylated C's in CpG context: 1
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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30 Total unmethylated C's in CHG context: 4
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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31 Total unmethylated C's in CHH context: 71
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32 Total unmethylated C's in Unknown context: 0
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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34 C methylated in CpG context: 50.0%
aa9bf0f29a9f "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit d85012b50faac3234496bb51e2a29c2d8c113bde"
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35 C methylated in CHG context: 42.9%
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36 C methylated in CHH context: 76.2%
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37 Can't determine percentage of methylated Cs in Unknown context (CN or CHN) if value was 0
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40 Bismark completed in 0d 0h 0m 7s