Bismark

Bismark Processing Report

input_1.fq.gz

Data processed at 10:50 on 2019-07-30


Alignment Stats

Sequences analysed in total 44115
Single-end alignments with a unique best hit 554
Sequences without alignments under any condition 43115
Sequences that did not map uniquely 446
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 8563
Methylated C's in CpG context 245
Methylated C's in CHG context 51
Methylated C's in CHH context 114
Methylated C's in Unknown context 1
Unmethylated C's in CpG context 133
Unmethylated C's in CHG context 1762
Unmethylated C's in CHH context 6258
Unmethylated C's in Unknown context 5
Percentage methylation (CpG context) 64.8%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 1.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 230 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 324 original bottom strand

Deduplication

Alignments analysed 554
Unique alignments 504
Duplicates removed 50
Duplicated alignments were found at 44 different positions

Cytosine Methylation after Extraction

Total C's analysed 8563
Methylated C's in CpG context 245
Methylated C's in CHG context 51
Methylated C's in CHH context 114
Unmethylated C's in CpG context 133
Unmethylated C's in CHG context 1762
Unmethylated C's in CHH context 6258
Percentage methylation (CpG context) 64.8%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 1.8%

M-Bias Plot