comparison correctGCBias.xml @ 24:d7c9fd76e41e draft

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author bgruening
date Tue, 04 Feb 2014 09:12:07 -0500
parents 8c452f37c896
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23:8c452f37c896 24:d7c9fd76e41e
43 43
44 ###set newoutFileName="corrected."+str($outFileFormat) 44 ###set newoutFileName="corrected."+str($outFileFormat)
45 ##--correctedFile $newoutFileName; 45 ##--correctedFile $newoutFileName;
46 --correctedFile "corrected.bam"; 46 --correctedFile "corrected.bam";
47 47
48 mv $newoutFileName $outFileName 48 mv "corrected.bam" $outFileName
49 </command> 49 </command>
50 <inputs> 50 <inputs>
51 <param name="GCbiasFrequenciesFile" type="data" format="tabular" label="Output of computeGCBias" /> 51 <param name="GCbiasFrequenciesFile" type="data" format="tabular" label="Output of computeGCBias" />
52 <param name="bamInput" format="bam" type="data" label="BAM file" help="This should be same file that was used for computeGCbias. The BAM file must be sorted."/> 52 <param name="bamInput" format="bam" type="data" label="BAM file" help="This should be same file that was used for computeGCbias. The BAM file must be sorted."/>
53 <expand macro="reference_genome_source" /> 53 <expand macro="reference_genome_source" />
86 </outputs> 86 </outputs>
87 <help> 87 <help>
88 88
89 **What it does** 89 **What it does**
90 90
91 This tool requires the output from computeGCBias to correct the given BAM files according to the method proposed by Benjamini and Speed (2012). Nucleic Acids Res. 91 This tool requires the output from computeGCBias to correct a given BAM file according to the method proposed by
92 The resulting BAM files can be used in any downstream analyses, but be aware that you should not filter out duplicates from here on. 92 Benjamini and Speed (2012) Nucleic Acids Res.
93 The resulting BAM file can be used in any downstream analyses, but be aware that you should not filter out duplicates from here on.
93 94
94 95 You can find more details on the correctGCBias wiki page: https://github.com/fidelram/deepTools/wiki/QC#wiki-correctGCbias
95 You can find more details in the `correctGCBias wiki`_.
96
97 .. _correctGCBias wiki: https://github.com/fidelram/deepTools/wiki/QC#wiki-correctGCbias
98 96
99 97
100 **Output files**: 98 **Output files**:
101 99
102 - GC-normalized BAM file 100 - GC-normalized BAM file