Mercurial > repos > bgruening > deeptools
comparison correctGCBias.xml @ 24:d7c9fd76e41e draft
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author | bgruening |
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date | Tue, 04 Feb 2014 09:12:07 -0500 |
parents | 8c452f37c896 |
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23:8c452f37c896 | 24:d7c9fd76e41e |
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43 | 43 |
44 ###set newoutFileName="corrected."+str($outFileFormat) | 44 ###set newoutFileName="corrected."+str($outFileFormat) |
45 ##--correctedFile $newoutFileName; | 45 ##--correctedFile $newoutFileName; |
46 --correctedFile "corrected.bam"; | 46 --correctedFile "corrected.bam"; |
47 | 47 |
48 mv $newoutFileName $outFileName | 48 mv "corrected.bam" $outFileName |
49 </command> | 49 </command> |
50 <inputs> | 50 <inputs> |
51 <param name="GCbiasFrequenciesFile" type="data" format="tabular" label="Output of computeGCBias" /> | 51 <param name="GCbiasFrequenciesFile" type="data" format="tabular" label="Output of computeGCBias" /> |
52 <param name="bamInput" format="bam" type="data" label="BAM file" help="This should be same file that was used for computeGCbias. The BAM file must be sorted."/> | 52 <param name="bamInput" format="bam" type="data" label="BAM file" help="This should be same file that was used for computeGCbias. The BAM file must be sorted."/> |
53 <expand macro="reference_genome_source" /> | 53 <expand macro="reference_genome_source" /> |
86 </outputs> | 86 </outputs> |
87 <help> | 87 <help> |
88 | 88 |
89 **What it does** | 89 **What it does** |
90 | 90 |
91 This tool requires the output from computeGCBias to correct the given BAM files according to the method proposed by Benjamini and Speed (2012). Nucleic Acids Res. | 91 This tool requires the output from computeGCBias to correct a given BAM file according to the method proposed by |
92 The resulting BAM files can be used in any downstream analyses, but be aware that you should not filter out duplicates from here on. | 92 Benjamini and Speed (2012) Nucleic Acids Res. |
93 The resulting BAM file can be used in any downstream analyses, but be aware that you should not filter out duplicates from here on. | |
93 | 94 |
94 | 95 You can find more details on the correctGCBias wiki page: https://github.com/fidelram/deepTools/wiki/QC#wiki-correctGCbias |
95 You can find more details in the `correctGCBias wiki`_. | |
96 | |
97 .. _correctGCBias wiki: https://github.com/fidelram/deepTools/wiki/QC#wiki-correctGCbias | |
98 | 96 |
99 | 97 |
100 **Output files**: | 98 **Output files**: |
101 | 99 |
102 - GC-normalized BAM file | 100 - GC-normalized BAM file |