diff bamCoverage.xml @ 27:bf1b1dcdd67b draft

Uploaded
author bgruening
date Mon, 17 Mar 2014 16:23:58 -0400
parents 2ad3b027dfcd
children f7712a057440
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--- a/bamCoverage.xml	Thu Feb 06 06:40:05 2014 -0500
+++ b/bamCoverage.xml	Mon Mar 17 16:23:58 2014 -0400
@@ -1,4 +1,4 @@
-<tool id="deeptools_bamCoverage" name="bamCoverage" version="1.0.4">
+<tool id="deeptools_bamCoverage" name="bamCoverage" version="1.0.5">
     <description> generates a coverage bigWig file from a given BAM file.  Multiple options are available to count reads and normalize coverage. (bam2bigwig)</description>
     <expand macro="requirements" />
     <expand macro="stdio" />
@@ -60,7 +60,7 @@
 
         <param name="fragmentLength" type="integer" value="300" min="1"
             label="Length of the average fragment size"
-            help ="Reads will be extended to match this length unless they are paired-end, in which case they will be extended to match the fragment length. If this value is set to the read length or smaller, the read will not be extended. *Warning* the fragment length affects the normalization to 1x (see &quot;normalize coverage to 1x&quot;). The formula to normalize using the sequencing depth is genomeSize/(number of mapped reads * fragment length). *NOTE*: If the BAM files contain mated and unmated paired-end reads, unmated reads will be extended to match the fragment length."/>
+            help ="Reads will be extended to match this length unless they are paired-end, in which case they will be extended to match the fragment length. If this value is set to the read length or smaller, the read will not be extended. *Warning* the fragment length affects the normalization to 1x (see &quot;normalize coverage to 1x&quot;). Sequencing depth is defined as: (total number of mapped reads * fragment length) / effective genome size. *NOTE*: If the BAM files contain mated and unmated paired-end reads, unmated reads will be extended to match the fragment length."/>
 
         <param name="binSize" type="integer" value="50" min="1" 
             label="Bin size in bp"