annotate bamCompare.xml @ 11:8f2a1286b61c draft

planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 999ec5d9b5882702497d8117a7d26536a67e19ca

<tool id="deeptools_bam_compare" name="bamCompare" version="@WRAPPER_VERSION@.0">
    <description>normalizes and compares two BAM files to obtain the ratio, log2ratio or difference between them</description>
    <macros>
        <token name="@BINARY@">bamCompare</token>
        <import>deepTools_macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <command>
<![CDATA[
        ln -s '$bamFile1' one.bam &&
        ln -s '${bamFile1.metadata.bam_index}' one.bam.bai &&
        ln -s '$bamFile2' two.bam &&
        ln -s '${bamFile2.metadata.bam_index}' two.bam.bai &&

        @BINARY@
            @THREADS@
            --bamfile1 one.bam
            --bamfile2 two.bam

            --outFileName '$outFileName'
            --outFileFormat '$outFileFormat'

            --binSize $binSize

            #if $scaling.method == 'SES':
                --scaleFactorsMethod SES
                --sampleLength $scaling.sampleLength
                --numberOfSamples $scaling.numberOfSamples
            #elif $scaling.method == 'readCount':
                --scaleFactorsMethod readCount
            #elif $scaling.method == 'own':
                --scaleFactors '$scaling.scaleFactor1:$scaling.scaleFactor2'
            #end if

            --ratio $comparison.type

            #if $comparison.type == 'subtract':
                #if $comparison.normalization.type == 'rpkm':
                    --normalizeUsingRPKM
                #elif $comparison.normalization.type == '1x':

                    #if $comparison.normalization.effectiveGenomeSize.effectiveGenomeSize_opt == "specific":
                        --normalizeTo1x $comparison.normalization.effectiveGenomeSize.effectiveGenomeSize
                    #else:
                        --normalizeTo1x $comparison.normalization.effectiveGenomeSize.effectiveGenomeSize_opt
                    #end if

                #end if
            #elif $comparison.type in ['ratio','log2']:
                --pseudocount $comparison.pseudocount
            #end if

            #if str($region).strip() != '':
                --region '$region'
            #end if

            #if $advancedOpt.showAdvancedOpt == "yes":
                #if $advancedOpt.smoothLength:
                    --smoothLength '$advancedOpt.smoothLength'
                #end if

                @ADVANCED_OPTS_READ_PROCESSING@
                $advancedOpt.skipNAs

                #if str($advancedOpt.ignoreForNormalization).strip() != '':
                    --ignoreForNormalization '$advancedOpt.ignoreForNormalization'
                #end if

                @blacklist@
            #end if
]]>
    </command>
    <inputs>
        <param argument="--bamFile1" format="bam" type="data" label="First BAM file (e.g. treated sample)"
            help=""/>
        <param argument="--bamFile2" format="bam" type="data" label="Second BAM file (e.g. control sample)"
            help=""/>

        <param argument="--binSize" type="integer" value="50" min="1"
            label="Bin size in bases"
            help="The genome will be divided into bins of the specified size. For each bin, the overlaping number of fragments (or reads) will be reported.
                If only half a fragment overlaps then this fraction will be reported."/>

        <conditional name="scaling">
            <param name="method" type="select"
                label="Method to use for scaling the largest sample to the smallest">
                <option value="readCount" selected="true">read count</option>
                <option value="SES">signal extraction scaling (SES), check with plotFingerprint before using it!</option>
                <option value="own">enter own scaling factors</option>
            </param>
            <when value="SES">
                <param argument="--sampleLength" type="integer" value="1000" min="10"
                    label="Length in bases used to sample the genome and compute the size or scaling factors."
                    help="The default is fine. Only change it if you know what you are doing." />
                <param argument="--numberOfSamples" type="integer" value="100000" min="0"
                    label="Number of samplings taken from the genome to compute the scaling factors"
                    help="" />
            </when>
            <when value="readCount" />
            <when value="own">
                <expand macro="scaleFactors" />
            </when>
        </conditional>

        <conditional name="comparison">
            <param name="type" type="select"
                label="How to compare the two files"
                help="The default is to output the log2ratio between the two samples.
                The reciprocal ratio returns the negative of the inverse of the ratio if
                the ratio is less than 0. The resulting values are interpreted as negative
                fold changes. To output the scaled values from the first or second BAM file,
                select 'first' or 'second'.">
                <option value="log2" selected="true">Compute log2 of the number of reads ratio</option>
                <option value="ratio">Compute the ratio of the number of reads</option>
                <option value="subtract">Compute difference (subtract input from treatment) of the number of reads</option>
                <option value="add">Compute the sum of number of reads</option>
                <option value="mean">Compute the mean of the number of reads between the two files</option>
                <option value="reciprocal_ratio">Computes the fold change. If the fold change is less than 1, the negative of the inverse is reported. E.g. A fold change of 10 to 5 would be reported not as 0.5 but as -2</option>
                <option value="first">Returns the scaled value of the first BAM file</option>
                <option value="second">Returns the scaled value of the second BAM file</option>
            </param>
            <when value="log2">
                <expand macro="pseudocount" />
            </when>
            <when value="ratio">
                <expand macro="pseudocount" />
            </when>
            <when value="add" />
            <when value="mean" />
            <when value="first" />
            <when value="second" />
            <when value="reciprocal_ratio">
                <expand macro="pseudocount" />
            </when>
            <when value="subtract">
                <conditional name="normalization">
                    <param name="type" type="select" label="Normalization method" >
                        <option value="1x">Normalize coverage to 1x</option>
                        <option value="rpkm">Normalize to fragments (reads) per kilobase per million (RPKM)</option>
                        <option value="no">Do not normalize or scale</option>
                    </param>
                    <when value="rpkm" />
                    <when value="no" />
                    <when value="1x">
                        <expand macro="effectiveGenomeSize" />
                    </when>
                </conditional>
            </when>
        </conditional>

        <param name="outFileFormat" type="select" label="Coverage file format">
            <option value="bigwig" selected="true">bigwig</option>
            <option value="bedgraph">bedgraph</option>
        </param>
        <expand macro="region_limit_operation" />
        <conditional name="advancedOpt">
            <param name="showAdvancedOpt" type="select" label="Show advanced options" >
                <option value="no" selected="true">no</option>
                <option value="yes">yes</option>
            </param>
            <when value="no" />
            <when value="yes">
                <expand macro="smoothLength" />
                <expand macro="read_processing_options" />

                <expand macro="skipNAs" />

                <param argument="--ignoreForNormalization" type="text" value="" size="50"
                    label="regions that should be excluded for calculating the scaling factor"
                    help="Sometimes it makes sense to exclude certain regions when calculating the scaling factor.
                        For example, if you know of copy number variations between samples then you may want to exclude these.
                        Another typical example is the difference in chromosome X copies between males and females in many species.
                        Example inputs are chrX,chrY,chr3 or chr10:12220-128932" />
                <expand macro="blacklist" />
            </when>
        </conditional>
    </inputs>
    <outputs>
        <data format="bigwig" name="outFileName">
        <change_format>
            <when input="outFileFormat" value="bigwig" format="bigwig" />
            <when input="outFileFormat" value="bedgraph" format="bedgraph" />
        </change_format>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="bamFile1" value="bowtie2 test1.bam" ftype="bam" />
            <param name="bamFile2" value="bowtie2 test1.bam" ftype="bam" />
            <param name="showAdvancedOpt" value="no" />
            <param name="outFileFormat" value="bigwig" />
            <param name="outFileFormat" value="bedgraph" />
            <param name="binSize" value="5" />
            <param name="type" value="ratio" />
            <output name="outFileName" file="bamCompare_result1.bg" ftype="bedgraph" />
        </test>
        <test>
            <param name="bamFile1" value="bowtie2 test1.bam" ftype="bam" />
            <param name="bamFile2" value="bowtie2 test1.bam" ftype="bam" />
            <param name="showAdvancedOpt" value="yes" />
            <param name="outFileFormat" value="bigwig" />
            <param name="outFileFormat" value="bigwig" />
            <param name="binSize" value="10" />
            <param name="type" value="ratio" />
            <output name="outFileName" file="bamCompare_result2.bw" ftype="bigwig" />
        </test>
    </tests>
    <help>
<![CDATA[

What it does
-------------

This tool can be used to generate a bigWig or bedGraph file based on **two BAM** files that are compared to each other while being simultaneously normalized for sequencing depth.

To compare the BAM files to each other, the genome is partitioned into bins of equal size, then the number of reads found in each BAM file is counted per bin, and finally a summary value reported.
This value can be the ratio of the number of reads per bin, the log2 of the ratio, or the difference.

This tool can normalize the number of reads in each BAM file using the SES method proposed in Diaz et al. (2012). "Normalization, bias correction, and peak
calling for ChIP-seq". Statistical applications in genetics and molecular
biology, 11(3).

Normalization based on read counts is also available.

By default, if reads are from a paired-end sequencing run and reads are properly paired, the fragment length reported in the BAM file is used.

**Note:** *For paired-end sequencing samples, each read mate is treated independently to avoid a bias when a mixture of concordant and discordant pairs are present. This means that _each end_ will be extended to match the fragment length.*

.. image:: $PATH_TO_IMAGES/norm_IGVsnapshot_indFiles.png
   :width: 600
   :height: 336

Output files
--------------

``bamCompare`` produces the same kind of output as ``bamCoverage``.
The difference is that you now obtain 1 coverage file that is based on 2 BAM files: a bedGraph or a bigwig file containing the bin location and the resulting comparison values.

Like BAM files, bigWig files are compressed, binary files. If you would like to see the coverage values, choose the bedGraph output. For more information on typical NGS file formats, see our `Glossary <http://deeptools.readthedocs.org/en/latest/content/help_glossary.html#file-formats>`_

.. image:: $PATH_TO_IMAGES/bamCompare_output.png
   :width: 600
   :height: 436

-----

@REFERENCES@
]]>
    </help>
    <expand macro="citations" />
</tool>