view plotFingerprint.xml @ 0:c1b46e0cb7ca draft

planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0a9265a12a303b54cdaa974e82e87c2ac60962ee-dirty

<tool id="deeptools_plot_fingerprint" name="plotFingerprint" version="@WRAPPER_VERSION@.0">
    <description>plots profiles of BAM files; useful for assesing ChIP signal strength</description>
    <macros>
        <token name="@BINARY@">plotFingerprint</token>
        <import>deepTools_macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <command>
<![CDATA[
        @multiple_input_bams@
        @BINARY@
            @THREADS@
            --bamfiles #echo " ".join($files)
            --labels #echo " ".join($labels)
            --plotFile $outFileName

            #if $output.showOutputSettings == "yes"
                --plotFileFormat $output.outFileFormat
                #if $output.saveRawCounts:
                    --outRawCounts '$outFileRawCounts'
                #end if
            #else
                --plotFileFormat 'png'
            #end if

            #if str($region).strip() != '':
                --region '$region'
            #end if

            #if $advancedOpt.showAdvancedOpt == "yes":
                --binSize '$advancedOpt.binSize'
                --numberOfSamples '$advancedOpt.numberOfSamples'

                $advancedOpt.ignoreDuplicates
                $advancedOpt.skipZeros

                #if $advancedOpt.minMappingQuality:
                --minMappingQuality '$advancedOpt.minMappingQuality'
                #end if

                #if $advancedOpt.plotTitle and str($advancedOpt.plotTitle.value) != "":
                    --plotTitle '$advancedOpt.plotTitle'
                #end if
                @ADVANCED_OPTS_READ_PROCESSING@
            #end if
]]>
    </command>

    <inputs>
        <expand macro="multiple_input_bams" />
        <expand macro="region_limit_operation" />

        <conditional name="advancedOpt">
            <param name="showAdvancedOpt" type="select" label="Show advanced options" >
                <option value="no" selected="true">no</option>
                <option value="yes">yes</option>
            </param>
            <when value="no" />
            <when value="yes">
                <param argument="--binSize" type="integer" value="500" min="1"
                   label="Bin size in bases"
                   help="Length in bases for a window used to sample the genome."/>
                <param argument="--numberOfSamples" type="integer" value="100000" min="1"
                   label="Number of samples"
                   help="Number of samples taken from the genome to compute the scaling factors."/>
                <expand macro="read_processing_options" />
                <expand macro="skipZeros" />
                <expand macro="plotTitle" />
            </when>
        </conditional>

        <conditional name="output">
            <param name="showOutputSettings" type="select" label="Show advanced output settings">
                <option value="no" selected="true">no</option>
                <option value="yes">yes</option>
            </param>
            <when value="no" />
            <when value="yes">
                <expand macro="input_image_file_format" />
                <param name="saveRawCounts" type="boolean" label="Save the bin counts" help="(--outRawCounts)"/>
            </when>
        </conditional>
    </inputs>
    <outputs>
        <expand macro="output_image_file_format" />
        <data format="tabular" name="outFileRawCounts" label="${tool.name} on ${on_string}: bin counts">
            <filter>
            ((
                output['showOutputSettings'] == 'yes' and
                output['saveRawCounts'] is True
            ))
            </filter>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="bamfiles" value="bowtie2-test1.bam,bowtie2-test1.bam" ftype="bam" />
            <param name="showAdvancedOpt" value="no" />
            <param name="showOutputSettings" value="no" />
            <output name="outFileName" file="plotFingerprint_result1.png" ftype="png" compare="sim_size" />
        </test>
        <test>
            <param name="bamfiles" value="bowtie2-test1.bam,bowtie2-test1.bam" ftype="bam" />
            <param name="showAdvancedOpt" value="yes" />
            <param name="showOutputSettings" value="yes" />
            <param name="saveRawCounts" value="True" />
            <param name="plotTitle" value="Test Fingerprint Plot" />
            <output name="outFileName" file="plotFingerprint_result2.png" ftype="png" compare="sim_size" />
            <output name="outFileRawCounts" file="plotFingerprint_result2.tabular" ftype="tabular" />
        </test>
    </tests>
    <help>
<![CDATA[


**What it does**

This tool is useful for assessing the strength of a ChIP (i.e. how clearly the enrichment signal can be separated from the background)
and is based on a method described in Diaz et al. (2012) Stat Appl Genet Mol Biol 11(3).

The tool first samples indexed BAM files and counts all reads overlapping a window (bin) of the specified length.
These counts are then sorted according to their rank (the bin with the highest number of reads has the highest rank)
and the cumulative sum of read counts are plotted. An ideal input (control) with a uniform distribution of reads alignments
result in a diagonal line. A very specific and strong ChIP enrichment, on the other hand, would result in a large portion
of reads accumulating in just a few bins and the resulting plot showing a steep rise toward the right-most edge. Such results are
most commonly seen with transcription factors.


.. image:: $PATH_TO_IMAGES/QC_fingerprint.png


You can find more details on the plotFingerprint doc page: https://deeptools.readthedocs.org/en/master/content/tools/plotFingerprint.html


**Output files**:

- Diagnostic plot
- (optional) Data matrix of raw counts

**Output with test dataset**:

What follows is the output of plotFingerprint with our test ChIP-Seq datasets, limiting analysis to chromosome X. Single-end reads were extended to
200 bp (advanced options).

.. image:: $PATH_TO_IMAGES/bamFP_galaxy_output.png


-----

@REFERENCES@
]]>
    </help>
    <expand macro="citations" />
</tool>