Mercurial > repos > bgruening > hicexplorer_chicplotviewpoint
comparison chicPlotViewpoint.xml @ 2:136ebddd6ec3 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 4b602d427e0fc0da5353a4510798349de98e4ae4"
author | iuc |
---|---|
date | Wed, 11 Mar 2020 17:14:05 -0400 |
parents | ae8bf5420df2 |
children | dbf82dd7bdb3 |
comparison
equal
deleted
inserted
replaced
1:61e2d34c5b99 | 2:136ebddd6ec3 |
---|---|
11 mkdir interactionFiles && | 11 mkdir interactionFiles && |
12 mkdir differentialFolder && | 12 mkdir differentialFolder && |
13 mkdir significantFolder && | 13 mkdir significantFolder && |
14 mkdir plots && | 14 mkdir plots && |
15 | 15 |
16 | 16 |
17 #for $file in $interactionFiles: | 17 #for $file in $interactionFiles: |
18 #set identifier = @ESCAPE_IDENTIFIER_FILE@ | 18 #set identifier = @ESCAPE_IDENTIFIER_FILE@ |
19 ln -s '$file' 'interactionFiles/$identifier' && | 19 ln -s '$file' 'interactionFiles/$identifier' && |
20 #end for | 20 #end for |
21 | 21 |
35 | 35 |
36 #if $batchmode_conditional.batchmode_selector == 'optionSinglemode': | 36 #if $batchmode_conditional.batchmode_selector == 'optionSinglemode': |
37 #set $interactonfileslist = ' '.join([ '\'interactionFiles/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $interactionFiles ]) | 37 #set $interactonfileslist = ' '.join([ '\'interactionFiles/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $interactionFiles ]) |
38 #set $differentialfileslist = ' '.join([ '\'differentialFolder/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $differentialFiles ]) | 38 #set $differentialfileslist = ' '.join([ '\'differentialFolder/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $differentialFiles ]) |
39 #set $significantfileslist = ' '.join([ '\'significantFolder/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $significantFiles ]) | 39 #set $significantfileslist = ' '.join([ '\'significantFolder/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $significantFiles ]) |
40 | 40 |
41 #end if | 41 #end if |
42 | 42 |
43 @BINARY@ | 43 @BINARY@ |
44 #if $batchmode_conditional.batchmode_selector == 'optionSinglemode': | 44 #if $batchmode_conditional.batchmode_selector == 'optionSinglemode': |
45 --interactionFile $interactonfileslist | 45 --interactionFile $interactonfileslist |
46 #if $differentialFiles: | 46 #if $differentialFiles: |
47 --differentialTestResult $differentialfileslist | 47 --differentialTestResult $differentialfileslist |
48 #end if | 48 #end if |
49 | 49 |
50 #if $significantFiles: | 50 #if $significantFiles: |
51 --significantInteractions $significantfileslist | 51 --significantInteractions $significantfileslist |
52 #end if | 52 #end if |
53 | 53 |
54 -o out_plot.$image_file_format | 54 -o out_plot.$image_file_format |
55 #else: | 55 #else: |
56 --interactionFile $batchmode_conditional.interactionFilesOrderFile | 56 --interactionFile $batchmode_conditional.interactionFilesOrderFile |
77 --backgroundModelFile $backgroundModelFile | 77 --backgroundModelFile $backgroundModelFile |
78 #end if | 78 #end if |
79 | 79 |
80 #if $dpi: | 80 #if $dpi: |
81 --dpi $dpi | 81 --dpi $dpi |
82 #end if | 82 #end if |
83 | 83 |
84 #if $maxPValue: | 84 #if $maxPValue: |
85 --maxPValue $maxPValue | 85 --maxPValue $maxPValue |
86 #end if | 86 #end if |
87 | 87 |
107 ]]></command> | 107 ]]></command> |
108 <inputs> | 108 <inputs> |
109 <param name='interactionFiles' type="data" format="interval" label="Interaction files" multiple="true"/> | 109 <param name='interactionFiles' type="data" format="interval" label="Interaction files" multiple="true"/> |
110 <param name='differentialFiles' type="data" format="interval" label="Rejected H0 files" multiple="true" optional='true'/> | 110 <param name='differentialFiles' type="data" format="interval" label="Rejected H0 files" multiple="true" optional='true'/> |
111 <param name='significantFiles' type="data" format="interval" label="Significant interaction files" multiple="true" optional='true'/> | 111 <param name='significantFiles' type="data" format="interval" label="Significant interaction files" multiple="true" optional='true'/> |
112 | 112 |
113 <param name="rangeUpstream" type="integer" value="500000" label="Upstream range" help='Defines the region upstream of a reference point which should be considered in the analysis.' /> | 113 <param name="rangeUpstream" type="integer" value="500000" label="Upstream range" help='Defines the region upstream of a reference point which should be considered in the analysis.' /> |
114 <param name="rangeDownstream" type="integer" value="500000" label="Downstream range" help='Defines the region upstream of a reference point which should be considered in the analysis.' /> | 114 <param name="rangeDownstream" type="integer" value="500000" label="Downstream range" help='Defines the region upstream of a reference point which should be considered in the analysis.' /> |
115 <param argument="--backgroundModelFile" type="data" format='tabular' | 115 <param argument="--backgroundModelFile" type="data" format='tabular' |
116 label="Background model" | 116 label="Background model" |
117 help="The background file computed by chicViewpointBackgroundModel" optional='true'/> | 117 help="The background file computed by chicViewpointBackgroundModel" optional='true'/> |
118 | 118 |
119 <conditional name="batchmode_conditional"> | 119 <conditional name="batchmode_conditional"> |
120 <param name="batchmode_selector" type="select" label="Compute files individually or in batch mode"> | 120 <param name="batchmode_selector" type="select" label="Compute files individually or in batch mode"> |
121 <option value="optionBatchmode">Batch processing</option> | 121 <option value="optionBatchmode">Batch processing</option> |
122 <option value="optionSinglemode" selected="True">Single file processing</option> | 122 <option value="optionSinglemode" selected="True">Single file processing</option> |
123 </param> | 123 </param> |
124 <when value="optionBatchmode"> | 124 <when value="optionBatchmode"> |
125 <param name='interactionFilesOrderFile' type="data" format="txt" label="Interaction file order"/> | 125 <param name='interactionFilesOrderFile' type="data" format="txt" label="Interaction file order"/> |
126 <param name='differentialFilesOrderFile' type="data" format="txt" label="Rejected H0 file order" optional='true'/> | 126 <param name='differentialFilesOrderFile' type="data" format="txt" label="Rejected H0 file order" optional='true'/> |
127 <param name='significantInteractionFilesOrderFile' type="data" format="txt" label="Significant file order" optional='true'/> | 127 <param name='significantInteractionFilesOrderFile' type="data" format="txt" label="Significant file order" optional='true'/> |
128 <param name='computeSampleNumber' type="integer" value='2' label="Plot n sample in one plot"/> | 128 <param name='computeSampleNumber' type="integer" value='2' label="Plot n sample in one plot"/> |
129 | 129 |
130 </when> | 130 </when> |
131 <when value="optionSinglemode"> | 131 <when value="optionSinglemode"> |
132 </when> | 132 </when> |
133 </conditional> | 133 </conditional> |
134 | 134 |
135 | 135 |
136 <param argument="--maxPValue" type="float" optional="true" label="Max p-value" help="Maximum value of the plotted p-value."/> | 136 <param argument="--maxPValue" type="float" optional="true" label="Max p-value" help="Maximum value of the plotted p-value."/> |
147 <option value="pdf">pdf</option> | 147 <option value="pdf">pdf</option> |
148 </param> | 148 </param> |
149 <param name='dpi' type='integer' label='DPI for image' help='Change the default resolution of the plot.' optional='true'/> | 149 <param name='dpi' type='integer' label='DPI for image' help='Change the default resolution of the plot.' optional='true'/> |
150 <param name='colorList' type='text' value='g b c m y k' label='Color list' help='Colorlist for the viewpoint lines. For all available colors please check matplotlib documentation: https://matplotlib.org/3.1.0/gallery/color/named_colors.html' optional='true'/> | 150 <param name='colorList' type='text' value='g b c m y k' label='Color list' help='Colorlist for the viewpoint lines. For all available colors please check matplotlib documentation: https://matplotlib.org/3.1.0/gallery/color/named_colors.html' optional='true'/> |
151 | 151 |
152 | 152 |
153 </inputs> | 153 </inputs> |
154 <outputs> | 154 <outputs> |
155 <collection name="plotsCollection" type="list" label="cHi-C viewpoint plots"> | 155 <collection name="plotsCollection" type="list" label="cHi-C viewpoint plots"> |
156 <discover_datasets pattern="__name_and_ext__" directory="plots" /> | 156 <discover_datasets pattern="__name_and_ext__" directory="plots" /> |
157 </collection> | 157 </collection> |
158 | 158 |
159 </outputs> | 159 </outputs> |
160 <tests> | 160 <tests> |
161 <test> | 161 <test> |
162 <param name="interactionFiles" value="cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_14300280_14300280_Eya1.bed,cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_4487435_4487435_Sox17.bed,cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_19093103_19093103_Tfap2d.bed,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_4487435_4487435_Sox17.bed,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_14300280_14300280_Eya1.bed,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d.bed"/> | 162 <param name="interactionFiles" value="cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_14300280_14300280_Eya1.txt,cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_4487435_4487435_Sox17.txt,cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_19093103_19093103_Tfap2d.txt,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_4487435_4487435_Sox17.txt,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_14300280_14300280_Eya1.txt,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d.txt"/> |
163 <param name="differentialFiles" value="cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_4487435_4487435_Sox17_H0_rejected.bed,cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_14300280_14300280_Eya1_H0_rejected.bed,cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d_H0_rejected.bed"/> | 163 <param name="differentialFiles" value="cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_4487435_4487435_Sox17_H0_rejected.txt,cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_14300280_14300280_Eya1_H0_rejected.txt,cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d_H0_rejected.txt"/> |
164 <param name="significantFiles" value="cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_4487435_4487435_Sox17_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_14300280_14300280_Eya1_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_19093103_19093103_Tfap2d_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_4487435_4487435_Sox17_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_14300280_14300280_Eya1_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d_output_significant.bed"/> | 164 <param name="significantFiles" value="cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_4487435_4487435_Sox17_output_significant.txt,cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_14300280_14300280_Eya1_output_significant.txt,cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_19093103_19093103_Tfap2d_output_significant.txt,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_4487435_4487435_Sox17_output_significant.txt,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_14300280_14300280_Eya1_output_significant.txt,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d_output_significant.txt"/> |
165 | 165 |
166 | 166 |
167 <conditional name="batchmode_conditional"> | 167 <conditional name="batchmode_conditional"> |
168 <param name="batchmode_selector" value='optionBatchmode'/> | 168 <param name="batchmode_selector" value='optionBatchmode'/> |
169 <param name="interactionFilesOrderFile" value='cHi-C/chicViewpoint/fileNames_two_matrices.txt'/> | 169 <param name="interactionFilesOrderFile" value='cHi-C/chicViewpoint/fileNames_two_matrices.txt'/> |
170 <param name="differentialFilesOrderFile" value='cHi-C/chicDifferentialTest/rejectedFilesList.txt'/> | 170 <param name="differentialFilesOrderFile" value='cHi-C/chicDifferentialTest/rejectedFilesList.txt'/> |
171 <param name="significantInteractionFilesOrderFile" value='cHi-C/chicSignificantInteractions/output_5_significant_files.txt'/> | 171 <param name="significantInteractionFilesOrderFile" value='cHi-C/chicSignificantInteractions/output_5_significant_files.txt'/> |
172 <param name='computeSampleNumber' value='2'/> | 172 <param name='computeSampleNumber' value='2'/> |
173 </conditional> | 173 </conditional> |
174 <param name="pvalue" value='True'/> | 174 <param name="pvalue" value='True'/> |
175 <param name="alpha" value='0.5'/> | 175 <param name="alpha" value='0.5'/> |
176 <param name='backgroundModelFile' value='cHi-C/background.bed'/> | 176 <param name='backgroundModelFile' value='cHi-C/background.txt'/> |
177 <param name='xFold' value='1.5'/> | 177 <param name='xFold' value='1.5'/> |
178 <output_collection name="plotsCollection" type="list" count="3"> | 178 <output_collection name="plotsCollection" type="list" count="3"> |
179 <element name="Eya1_FL-E13-5_MB-E10-5_chr1_chr1_14300280" file="cHi-C/chicPlotViewpoint/batchMode/two/Eya1_FL-E13-5_MB-E10-5_chr1_chr1_14300280.png" ftype="png" compare='sim_size' delta='40000'/> | 179 <element name="Eya1_FL-E13-5_MB-E10-5_chr1_chr1_14300280" file="cHi-C/chicPlotViewpoint/batchMode/two/Eya1_FL-E13-5_MB-E10-5_chr1_chr1_14300280.png" ftype="png" compare='sim_size' delta='40000'/> |
180 <element name="Sox17_FL-E13-5_MB-E10-5_chr1_chr1_4487435" file="cHi-C/chicPlotViewpoint/batchMode/two/Sox17_FL-E13-5_MB-E10-5_chr1_chr1_4487435.png" ftype="png" compare='sim_size' delta='40000'/> | 180 <element name="Sox17_FL-E13-5_MB-E10-5_chr1_chr1_4487435" file="cHi-C/chicPlotViewpoint/batchMode/two/Sox17_FL-E13-5_MB-E10-5_chr1_chr1_4487435.png" ftype="png" compare='sim_size' delta='40000'/> |
181 <element name="Tfap2d_FL-E13-5_MB-E10-5_chr1_chr1_19093103" file="cHi-C/chicPlotViewpoint/batchMode/two/Tfap2d_FL-E13-5_MB-E10-5_chr1_chr1_19093103.png" ftype="png" compare='sim_size' delta='40000'/> | 181 <element name="Tfap2d_FL-E13-5_MB-E10-5_chr1_chr1_19093103" file="cHi-C/chicPlotViewpoint/batchMode/two/Tfap2d_FL-E13-5_MB-E10-5_chr1_chr1_19093103.png" ftype="png" compare='sim_size' delta='40000'/> |
182 </output_collection> | 182 </output_collection> |
183 | 183 |
184 </test> | 184 </test> |
185 <test> | 185 <test> |
186 <param name="interactionFiles" value="cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_14300280_14300280_Eya1.bed"/> | 186 <param name="interactionFiles" value="cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_14300280_14300280_Eya1.txt"/> |
187 | 187 |
188 <conditional name="batchmode_conditional"> | 188 <conditional name="batchmode_conditional"> |
189 <param name="batchmode_selector" value='optionSinglemode'/> | 189 <param name="batchmode_selector" value='optionSinglemode'/> |
190 </conditional> | 190 </conditional> |
191 | 191 |
192 <output_collection name="plotsCollection" type="list" count="1"> | 192 <output_collection name="plotsCollection" type="list" count="1"> |
198 <help><![CDATA[ | 198 <help><![CDATA[ |
199 | 199 |
200 Plot of viewpoints | 200 Plot of viewpoints |
201 ================== | 201 ================== |
202 | 202 |
203 chicPlotViewpoint plots one or many viewpoints with the average background model and the computed p-value per sample. Moreover it can highlight differential interactions of two samples and or significant regions. | 203 chicPlotViewpoint plots one or many viewpoints with the average background model and the computed p-value per sample. Moreover it can highlight differential interactions of two samples and/or significant regions. |
204 | 204 |
205 An example usage is: | 205 An example usage is: |
206 | 206 |
207 `$ chicPlotViewpoint --interactionFile viewpoint1.bed viewpoint2.bed --range 500000 500000 --backgroundModelFile background_model.bed --pValue --outFileName viewpoint1_2.png --dpi 300` | 207 `$ chicPlotViewpoint --interactionFile viewpoint1.txt viewpoint2.txt --range 500000 500000 --backgroundModelFile background_model.txt --pValue --outFileName viewpoint1_2.png --dpi 300` |
208 | 208 |
209 | 209 |
210 In batch mode the list of file names and the folders containing the files need to be given: | 210 In batch mode the list of file names and the folders containing the files need to be given: |
211 | 211 |
212 `$ chicPlotViewpoint --interactionFile viewpoint_names.txt -interactionFileFolder viewpointFilesFolder --differentialTestResult rejected_H0.txt --differentialTestResultsFolder differentialFolder --range 500000 500000 --backgroundModelFile background_model.bed --pValue --outputFolder plotsFOlder --dpi 300 --threads 20` | 212 `$ chicPlotViewpoint --interactionFile viewpoint_names.txt -interactionFileFolder viewpointFilesFolder --differentialTestResult rejected_H0.txt --differentialTestResultsFolder differentialFolder --range 500000 500000 --backgroundModelFile background_model.txt --pValue --outputFolder plotsFOlder --dpi 300 --threads 20` |
213 | 213 |
214 | 214 |
215 For more information about HiCExplorer please consider our documentation on readthedocs.io_ | 215 For more information about HiCExplorer please consider our documentation on readthedocs.io_ |
216 | 216 |
217 .. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html | 217 .. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html |