Mercurial > repos > bgruening > hicexplorer_hicmergedomains
diff hicMergeDomains.xml @ 0:6b9b4fd51308 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 55de003830db380639df997cdc5810df8022f63e"
author | bgruening |
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date | Tue, 16 Mar 2021 19:37:51 +0000 |
parents | |
children | b376ef118807 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/hicMergeDomains.xml Tue Mar 16 19:37:51 2021 +0000 @@ -0,0 +1,87 @@ +<tool id="hicexplorer_hicmergedomains" name="@BINARY@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@"> + <description>Merges TAD domains</description> + <macros> + <token name="@BINARY@">hicMergeDomains</token> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <command detect_errors="exit_code"><![CDATA[ + + #for $counter, $tadfile in enumerate($domainFiles): + ln -s '$tadfile' '${counter}_tad_file.bed' && + #end for + #set $m = ' '.join([ '\'%s_tad_file.bed\'' % $counter for $counter, $inputfile in enumerate($domainFiles) ]) + + @BINARY@ + + --domainFiles $m + + #if $proteinFile: + --proteinFile '$proteinFile' + #end if + + #if $minimumNumberOfPeaks: + --minimumNumberOfPeaks $minimumNumberOfPeaks + #end if + + #if $valueX: + --value $valueX + #end if + + #if $percent: + --percent $percent + #end if + + --outputMergedList mergedDomains.bed + --outputRelationList relationList.txt + --outputTreePlotFormat pdf + --outputTreePlotPrefix relationship_tree + + && mkdir relationship_plots + && cp relationship_tree_*.pdf relationship_plots +]]> + </command> + <inputs> + <param name="domainFiles" type='data' format="bed" multiple="true" label="Domain files from hicFindTADs of different resolutions to include" /> + <param argument="--proteinFile" type='data' format="bed" label="Protein peak file in bed format" help= "In order to be able to better assess the relationship between TADs, the associated protein file (e.g. CTCF for mammals) can be included. The protein file is required in broadpeak forma" /> + <param argument="--minimumNumberOfPeaks" type="integer" label="Minimum number of peaks" help="Optional parameter to adjust the number of protein peaks when adapting the resolution to the domain files. At least minimumNumberOfPeaks of unique peaks must be in a bin to considered. Otherwise the bin is treated like it has no peaks" value='1' /> + <param argument="--valueX" type="integer" label="TAD seperation value" help="Determine a value by how much the boundaries of two TADs must at least differ to consider them as two separate TADs" value='5000' /> + <param argument="--percent" type="float" label="Percentage overlap" help="For the relationship determination, a percentage is required from which area coverage the TADs are related to each other. For example, a relationship should be entered from 5 percent area coverage" value="0.5" /> + </inputs> + <outputs> + <data name='outputMergedList' from_work_dir='mergedDomains.bed' format='bed' label='Merged domains' /> + <data name='outputRelationList' from_work_dir='relationList.txt' format='txt' label='Relationship list' /> + <collection name="plotCollection" type="list" label="Relationship tree plots"> + <discover_datasets pattern="__name__" format='pdf' directory="relationship_plots" /> + </collection> + </outputs> + <tests> + <test> + <param name="domainFiles" ftype='bed' value="hicMergeDomains/10kbtad_domains.bed,hicMergeDomains/50kbtad_domains.bed,hicMergeDomains/100kbtad_domains.bed" /> + + <param name="minimumNumberOfPeaks" value="2" /> + <param name="proteinFile" value="hicMergeDomains/ctcf_sorted.bed" /> + <param name="valueX" value="5000" /> + <param name="percent" value="0.7" /> + <output name="outputMergedList" file="hicMergeDomains/merged_list.bed" ftype="bed" /> + <output name="outputRelationList" file="hicMergeDomains/relationship.txt" ftype="txt" /> + + <output_collection name="plotCollection" type="list" count="1"> + <element name="relationship_tree_1.pdf" file="hicMergeDomains/ctcf_plot/two_files_plot_1.pdf" ftype="pdf" compare="sim_size" /> + </output_collection> + </test> + </tests> + <help><![CDATA[ + +Merge of TAD domains +==================== + +Merges and computes the hierachy of TADs from different resolutions. + +For more information about HiCExplorer please consider our documentation on readthedocs.io_. + +.. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html + +]]> </help> + <expand macro="citations" /> +</tool>