comparison imagej2_adjust_threshold_binary_jython_script.py @ 0:018144807556 draft default tip

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8f49f3c66b5a1de99ec15e65c2519a56792f1d56
author bgruening
date Tue, 24 Sep 2024 17:12:52 +0000
parents
children
comparison
equal deleted inserted replaced
-1:000000000000 0:018144807556
1 import sys
2
3 from ij import IJ, Prefs
4
5 # Fiji Jython interpreter implements Python 2.5 which does not
6 # provide support for argparse.
7 input_file = sys.argv[-8]
8 threshold_min = float(sys.argv[-7])
9 threshold_max = float(sys.argv[-6])
10 method = sys.argv[-5]
11 display = sys.argv[-4]
12 black_background = sys.argv[-3] == "yes"
13 output_filename = sys.argv[-2]
14 output_datatype = sys.argv[-1]
15
16 # Open the input image file.
17 input_image_plus = IJ.openImage(input_file)
18
19 # Create a copy of the image.
20 input_image_plus_copy = input_image_plus.duplicate()
21
22 bit_depth = input_image_plus_copy.getBitDepth()
23
24 if black_background:
25 Prefs.blackBackground = True
26 else:
27 Prefs.blackBackground = False
28
29 if method != "Manual":
30 # Set the options.
31 if black_background:
32 method_str = "%s dark" % method
33 suffix = "black"
34 else:
35 method_str = method
36 suffix = ""
37 threshold_min = 1
38 # Set threshold_max based on image bit-depth
39 # For 8-bit images, use 255; for 16-bit images, use 65535
40 if bit_depth == 8:
41 threshold_max = 255 # Default for 8-bit images
42 elif bit_depth == 16:
43 threshold_max = 65535 # Default for 16-bit images
44 else:
45 threshold_max = float('inf') # General fallback if bit depth is unknown
46
47 IJ.setAutoThreshold(input_image_plus_copy, method_str)
48 IJ.run(input_image_plus_copy, "Convert to Mask", "calculate %s" % suffix)
49 if display == "red":
50 display_mode = "Red"
51 elif display == "bw":
52 display_mode = "Black & White"
53 elif display == "over_under":
54 display_mode = "Over/Under"
55 IJ.setThreshold(input_image_plus_copy, threshold_min, threshold_max, display_mode)
56 # Save the ImagePlus object as a new image.
57 IJ.saveAs(input_image_plus_copy, output_datatype, output_filename)