changeset 0:652f9d550531 draft

Uploaded
author bgruening
date Tue, 22 Apr 2014 13:56:22 -0400
parents
children 55bb96edfc07
files cmbuild.xml cmsearch.xml cmstat.xml datatypes_conf.xml infernal.py readme.rst repository_dependencies.xml tool-data/infernal.loc.sample tool_dependencies.xml
diffstat 9 files changed, 864 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/cmbuild.xml	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,271 @@
+<tool id="infernal_cmbuild" name="Build covariance models" version="1.1.0.1">
+    <description>from sequence alignments (cmbuild)</description>
+    <parallelism method="multi" split_inputs="alignment_infile" split_mode="to_size" split_size="10" shared_inputs="" merge_outputs="cmfile_outfile"></parallelism>
+    <requirements>
+        <requirement type="package">infernal</requirement>
+        <requirement type="package" version="1.1">infernal</requirement>
+        <requirement type="package" version="8.21">gnu_coreutils</requirement>
+    </requirements>
+    <command>
+        cmbuild 
+            #if $is_summery_output:
+                -o $summary_outfile
+            #end if
+            
+            ## to many outputs, is that one really needed?
+            ##-O $annotated_source_alignment_outfile
+
+            $model_construction_opts.model_construction_opts_selector
+            #if $model_construction_opts.model_construction_opts_selector == '--fast':
+                --symfrac $model_construction_opts.symfrac
+            #end if
+
+            $noss
+
+            $relative_weights_opts.relative_weights_opts_selector
+            #if $relative_weights_opts.relative_weights_opts_selector == '--wblosum':
+                --wid $relative_weights_opts.wid
+            #end if
+
+            $effective_opts.effective_opts_selector
+            #if str($effective_opts.effective_opts_selector) == '--eent':
+                --ere $effective_opts.ere
+                --eminseq $effective_opts.eminseq
+                --ehmmre $effective_opts.ehmmre
+                --eset $effective_opts.eset
+            #end if
+
+            #if str($refining_opts.refining_opts_selector) == '--refine':
+                #if $refining_opts.refine_output:
+                    --refine $refined_multiple_alignment_output
+                #else:
+                    --refine /dev/null
+                #end if
+
+                $l
+                $refining_opts.gibbs_opts.gibbs_opts_selector
+
+                #if str($refining_opts.gibbs_opts.gibbs_opts_selector) == '--gibbs':
+                    $refining_opts.gibbs_opts.random_seed
+                #end if
+
+                $notrunc
+                $cyk
+            #end if
+
+            $cmfile_outfile
+            $alignment_infile
+            
+    </command>
+        <inputs>
+            <!-- Stockholm or SELEX 
+            SELEX is defined in EMBOSS datatypes
+            -->
+            <param name="alignment_infile" type="data" format="stockholm,selex" label="Sequence database"/>
+
+            <conditional name="model_construction_opts">
+                <param name="model_construction_opts_selector" type="select" label="These options control how consensus columns are defined in an alignment" help="">
+                    <option value="--fast" selected="true">automatic (--fast)</option>
+                    <option value="--hand">user defined (--hand)</option>
+                </param>
+                <when value="--fast">
+                    <param name="symfrac" type="float" value="0.5" size="5" 
+                        label="Define the residue fraction threshold necessary to define a consensus (--symfrac)" help=""/>
+                </when>
+                <when value="--hand"/>
+            </conditional>
+
+            <param name="noss" truevalue="--noss" falsevalue="" checked="False" type="boolean" 
+                label="Ignore the secondary structure annotation, if any, in your multiple alignment file (--noss)" help=""/>
+
+            <conditional name="relative_weights_opts">
+                <param name="relative_weights_opts_selector" type="select" label="Options controlling relative weights" help="">
+                    <option value="--wpb" selected="true">Henikoff (--wgb)</option>
+                    <option value="--wgsc">Gerstein/Sonnhammer/Chothia (--wgsc)</option>
+                    <option value="--wnone">no sequence weighting (--wnone)</option>
+                    <option value="--wgiven">Sequence weight from given in input file (--wgiven)</option>
+                    <option value="--wblosum">BLOSUM filtering algorithm (--wblosum)</option>
+                </param>
+                <when value="--wpb"/>
+                <when value="--wgsc"/>
+                <when value="--wnone"/>
+                <when value="--wgiven"/>
+                <when value="--wblosum">
+                    <param name="wid" type="float" value="0.5" size="5" 
+                        label="Percent identity for clustering the alignment (--wid)" help=""/>
+                </when>
+            </conditional>
+
+
+            <conditional name="effective_opts">
+                <param name="effective_opts_selector" type="select" label="Options controlling effective sequence number" help="">
+                    <option value="--eent" selected="true">entropy weighting strategy (--eent)</option>
+                    <option value="--enone">Turn off the entropy weighting strategy (--enone)</option>
+                </param>
+                <when value="--enone"/>
+                <when value="--eent">
+                    <param name="ere" type="float" value="0.59" size="5" 
+                        label="Set the target mean match state relative entropy (--ere)" help=""/>
+
+                    <param name="eminseq" type="integer" value="" size="5" 
+                        label="Define the minimum allowed effective sequence number (--eminseq)" help=""/>
+
+                    <param name="ehmmre" type="float" value="" size="5" 
+                        label="Set the target HMM mean match state relative entropy (--ehmmre)" help=""/>
+
+                    <param name="eset" type="integer" value="" size="5" 
+                        label="Set the effective sequence number for entropy weighting (--eset)" help=""/>
+                </when>
+            </conditional>
+
+
+            <conditional name="refining_opts">
+                <param name="refining_opts_selector" type="select" label="Options for refining the input alignment" help="">
+                    <option value="" selected="true">No refinement</option>
+                    <option value="--refine">refine the input alignment</option>
+                </param>
+                <when value=""/>
+                <when value="--refine">
+
+                    <conditional name="gibbs_opts">
+                        <param name="gibbs_opts_selector" type="select" label="refinement mode" help="">
+                            <option value="" selected="true">expectation-maximization (EM)</option>
+                            <option value="--gibbs">Gibbs sampling</option>
+                        </param>
+                        <when value=""/>
+                        <when value="--gibbs">
+                            <param name="random_seed" type="integer" value="0" label="Randam Seed" help="" />
+                        </when>
+                    </conditional>
+
+                    <param name="l" truevalue="-l" falsevalue="" checked="False" type="boolean" 
+                        label="Turn on the local alignment algorithm" help="... which allows the alignment to span two or more subsequences if necessary"/>
+
+                    <param name="notrunc" truevalue="--notrunc" falsevalue="" checked="False" type="boolean" 
+                        label="Turn off the truncated alignment algorithm" help=""/>
+
+                    <param name="cyk" type="select" label="Options for refining the input alignment" help="">
+                        <option value="" selected="true">optimal accuracy algorithm</option>
+                        <option value="--cyk">align with the CYK algorithm</option>
+                    </param>
+
+                    <param name="refine_output" truevalue="" falsevalue="" checked="False" type="boolean" 
+                        label="Output the refined alignment file as it is used to build the covariance model" help=""/>
+
+                </when>
+            </conditional>
+
+
+            <param name="is_summery_output" truevalue="" falsevalue="" checked="False" type="boolean" 
+                label="Output a summery file?" help=""/>
+
+        </inputs>
+    <outputs>
+
+        <data format="text" name="summary_outfile" label="cmbuild summary on ${on_string}">
+            <filter>is_summery_output is True</filter>
+        </data>
+        <!--<data format="stockholm" name="annotated_source_alignment_outfile" label="Annotated alignment from ${on_string}"/>-->
+        <data format="cm" name="cmfile_outfile" label="Covariance models from ${on_string}"/>
+
+        <data format="stockholm" name="refined_multiple_alignment_output" label="refined alignment file of ${on_string}">
+            <filter>
+                ((
+                refining_opts['refining_opts_selector'] == "--refine" and
+                refining_opts['refine_output'] is True
+                ))
+            </filter>
+        </data>
+
+    </outputs>
+    <help>
+
+
+**What it does**
+
+For each multiple sequence alignment build a covariance model.
+The alignment file must be in Stockholm or SELEX format, and must contain consensus secondary structure annotation.
+cmbuild uses the consensus structure to determine the architecture of the CM.
+
+In addition to writing CM(s) to CMFILE_OUT, cmbuild also outputs a single line for each model created to stdout. Each
+line has the following fields: ”aln”: the index of the alignment used to build the CM; ”idx”: the index of the CM in the
+CMFILE_OUT; ”name”: the name of the CM; ”nseq”: the number of sequences in the alignment used to build the CM;
+”eff nseq”: the effective number of sequences used to build the model; ”alen”: the length of the alignment used to build
+the CM; ”clen”: the number of columns from the alignment defined as consensus (match) columns; ”bps”: the number
+of basepairs in the CM; ”bifs”: the number of bifurcations in the CM; ”rel entropy: CM”: the total relative entropy of the
+model divided by the number of consensus columns; ”rel entropy: HMM”: the total relative entropy of the model ignoring
+secondary structure divided by the number of consensus columns. ”description”: description of the model/alignment.
+
+
+Options controlling model construction
+--------------------------------------
+
+These options control how consensus columns are defined in an alignment.
+
+  * --fast Define consensus columns automatically as those that have a fraction >= symfrac of residues as opposed to gaps. (See below for the --symfrac option.) This is the default.
+  * --hand Use reference coordinate annotation (#=GC RF line, in Stockholm) to determine which columns are consensus, and which are inserts. Any non-gap character indicates a consensus column. (For example, mark consensus columns with ”x”, and insert columns with ”.”.)
+  * --symfrac Define the residue fraction threshold necessary to define a consensus column when not using --hand. The default is 0.5. The symbol fraction in each column is calculated after taking relative sequence weighting into account. Setting this to 0.0 means that every alignment column will be assigned as consensus, which may be useful in some cases. Setting it to 1.0 means that only columns that include 0 gaps will be assigned as consensus.
+  * --noss Ignore the secondary structure annotation, if any, in MSA-Infile and build a CM with zero basepairs. This model will be similar to a profile HMM and the cmsearch and cmscan programs will use HMM algorithms which are faster than CM ones for this model. Additionally, a zero basepair model need not be calibrated with cmcalibrate prior to running cmsearch with it. The --noss option must be used if there is no secondary structure annotation in MSA-Infile.
+
+
+Options controlling relative weights
+------------------------------------
+
+cmbuild uses an ad hoc sequence weighting algorithm to downweight closely related sequences and upweight distantly
+related ones. This has the effect of making models less biased by uneven phylogenetic representation. For example,
+two identical sequences would typically each receive half the weight that one sequence would. These options control
+which algorithm gets used.
+
+  * --wpb Use the Henikoff position-based sequence weighting scheme [Henikoff and Henikoff, J. Mol. Biol. 243:574, 1994]. This is the default.
+  * --wgsc Use the Gerstein/Sonnhammer/Chothia weighting algorithm [Gerstein et al, J. Mol. Biol. 235:1067, 1994].
+  * --wnone Turn sequence weighting off; e.g. explicitly set all sequence weights to 1.0.
+  * --wgiven Use sequence weights as given in annotation in the input alignment file. If no weights were given, assume they are all 1.0. The default is to determine new sequence weights by the Gerstein/Sonnhammer/Chothia algorithm, ignoring any annotated weights.
+  * --wblosum Use the BLOSUM filtering algorithm to weight the sequences, instead of the default GSC weighting. Cluster the sequences at a given percentage identity (see --wid); assign each cluster a total weight of 1.0, distributed equally amongst the members of that cluster.
+  * --wid Controls the behavior of the --wblosum weighting option by setting the percent identity for clustering the alignment.
+
+
+Options controlling effective sequence number
+---------------------------------------------
+
+After relative weights are determined, they are normalized to sum to a total effective sequence number, eff nseq. This
+number may be the actual number of sequences in the alignment, but it is almost always smaller than that. The default
+entropy weighting method (--eent) reduces the effective sequence number to reduce the information content (relative
+entropy, or average expected score on true homologs) per consensus position. The target relative entropy is controlled
+by a two-parameter function, where the two parameters are settable with --ere and --esigma.
+
+  * --eent Use the entropy weighting strategy to determine the effective sequence number that gives a target mean match state relative entropy. This option is the default, and can be turned off with --enone. The default target mean match state relative entropy is 0.59 bits for models with at least 1 basepair and 0.38 bits for models with zero basepairs, but changed with --ere. The default of 0.59 or 0.38 bits is automatically changed if the total relative entropy of the model (summed match state relative entropy) is less than a cutoff, which is is 6.0 bits by default, but can be changed with the expert, undocumented --eX option. If you really want to play with that option, consult the source code.
+  * --enone Turn off the entropy weighting strategy. The effective sequence number is just the number of sequences in the alignment.
+  * --ere Set the target mean match state relative entropy. By default the target relative entropy per match position is 0.59 bits for models with at least 1 basepair and 0.38 for models with zero basepairs.
+  * --eminseq Define the minimum allowed effective sequence number.
+  * --ehmmre Set the target HMM mean match state relative entropy. Entropy for basepairing match states is calculated using marginalized basepair emission probabilities.
+  * --eset Set the effective sequence number for entropy weighting.
+
+
+
+Options for refining the input alignment
+----------------------------------------
+
+  * --refine Attempt to refine the alignment before building the CM using expectation-maximization (EM). A CM is first built from the initial alignment as usual. Then, the sequences in the alignment are realigned optimally (with the HMM banded CYK algorithm, optimal means optimal given the bands) to the CM, and a new CM is built from the resulting alignment. The sequences are then realigned to the new CM, and a new CM is built from that alignment. This is continued until convergence, specifically when the alignments for two successive iterations are not significantly different (the summed bit scores of all the sequences in the alignment changes less than 1% between two successive iterations).
+  * -l Turn on the local alignment algorithm, which allows the alignment to span two or more subsequences if necessary (e.g. if the structures of the query model and target sequence are only partially shared), allowing certain large insertions and deletions in the structure to be penalized differently than normal indels. The default is to globally align the query model to the target sequences.
+  * --gibbs Modifies the behavior of --refine so Gibbs sampling is used instead of EM. The difference is that during the alignment stage the alignment is not necessarily optimal, instead an alignment (parsetree) for each sequences is sampled from the posterior distribution of alignments as determined by the Inside algorithm. Due to this sampling step --gibbs is non- deterministic, so different runs with the same alignment may yield different results. This is not true when --refine is used without the --gibbs option, in which case the final alignment and CM will always be the same. When --gibbs is enabled, the --seed "number" option can be used to seed the random number generator predictably, making the results reproducible. The goal of the --gibbs option is to help expert RNA alignment curators refine structural alignments by allowing them to observe alternative high scoring alignments.
+  * --seed Seed the random number generator with an integer >= 0. This option can only be used in combination with --gibbs. If the given number is nonzero, stochastic sampling of alignments will be reproducible; the same command will give the same results. If the given number is 0, the random number generator is seeded arbitrarily, and stochastic samplings may vary from run to run of the same command. The default seed is 0.
+  * --cyk With --refine, align with the CYK algorithm. By default the optimal accuracy algorithm is used. There is more information on this in the cmalign manual page.
+  * --notrunc With --refine, turn off the truncated alignment algorithm. There is more information on this in the cmalign manual page.
+
+
+For further questions please refere to the Infernal Userguide_.
+
+.. _Userguide: http://selab.janelia.org/software/infernal/Userguide.pdf
+
+
+How do I cite Infernal?
+-----------------------
+
+The recommended citation for using Infernal 1.1 is E. P. Nawrocki and S. R. Eddy, Infernal 1.1: 100-fold faster RNA homology searches , Bioinformatics 29:2933-2935 (2013). 
+
+**Galaxy Wrapper Author**::
+
+    *  Bjoern Gruening, University of Freiburg
+
+    </help>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/cmsearch.xml	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,254 @@
+<tool id="infernal_cmsearch" name="Search covariance model(s)" version="1.1.0.2">
+    <description>against a sequence database (cmsearch)</description>
+    <parallelism method="multi" split_inputs="seqdb" split_mode="to_size" split_size="100" shared_inputs="" merge_outputs="outfile,multiple_alignment_output"></parallelism>
+    <requirements>
+        <requirement type="package">infernal</requirement>
+        <requirement type="package" version="1.1">infernal</requirement>
+        <requirement type="package" version="8.21">gnu_coreutils</requirement>
+    </requirements>
+    <command>
+        ## a temp file is needed, because the standard tabular output from infernal is not usefull in Galaxy
+        ## it will be converted to a tab delimited file and piped to Galaxy
+        temp_tabular_output=\$(mktemp);
+
+        cmsearch 
+            ## Infernal Options
+            --cpu "\${GALAXY_SLOTS:-12}"
+            -o /dev/null
+            --tformat $seqdb.ext ##target format: fasta, embl, genbank, ddbj, stockholm, pfam, a2m, afa, clustal, and phylip 
+            $bottomonly
+            $toponly
+            $cyk
+            $notrunc
+            $max
+            $nohmm
+            $mid
+            ##$bitscore_thresholds
+            --tblout \$temp_tabular_output
+            $g
+            #if $A:
+                $A $multiple_alignment_output
+            #end if
+
+            #if str($inclusion_thresholds_opts.inclusion_thresholds_selector) == "--incE":
+                --incE $inclusion_thresholds_opts.incE
+            #elif str($inclusion_thresholds_opts.inclusion_thresholds_selector) == "--incT":
+                --incT $inclusion_thresholds_opts.incT
+            #end if
+
+            #if str($reporting_thresholds_opts.reporting_thresholds_selector) == "-E":
+                -E $reporting_thresholds_opts.E
+            #elif str($reporting_thresholds_opts.reporting_thresholds_selector) == "-T":
+                -T $reporting_thresholds_opts.T
+            #end if
+
+            ## CM file from the history or stored as database on disc
+
+            #if str($cm_opts.cm_opts_selector) == "db":
+                $cm_opts.database.fields.path
+            #else:
+                $cm_opts.cmfile
+            #end if
+
+            ## sequence file
+            $seqdb
+            2>&#38;1
+            ;
+
+            ## 1. replace all lines starting # (comment lines)
+            ## 2. replace the first 18 spaces with tabs, 18th field is a free text field (can contain spaces)
+            sed -e 's/#.*$//' -e '/^$/d' -e 's/ /\t/g' -e 's/\t/ /18g' \$temp_tabular_output > $outfile
+
+    </command>
+        <inputs>
+
+            <param name="seqdb" type="data" format="fasta" label="Sequence database"/>
+
+            <conditional name="cm_opts">
+                <param name="cm_opts_selector" type="select" label="Subject covariance models">
+                  <option value="db" selected="True">Locally installed covariance models</option>
+                  <option value="histdb">Covariance model from your history</option>
+                </param>
+                <when value="db">
+                    <param name="database" type="select" label="Covariance models">
+                        <options from_file="infernal.loc">
+                          <column name="value" index="0"/>
+                          <column name="name" index="1"/>
+                          <column name="path" index="2"/>
+                        </options>
+                    </param>
+                </when>
+                <when value="histdb">
+                    <param name="cmfile" type="data" format="cm" label="Covariance models file from the history."/>
+                </when>
+            </conditional>
+
+            <param name="g" truevalue="-g" falsevalue="" checked="False" type="boolean" 
+                label="Turn on the glocal alignment algorithm" help="... global with respect to the query model and local with respect to the target database."/>
+
+            <param name="bottomonly" truevalue="--bottomonly" falsevalue="" checked="False" type="boolean" 
+                label="Only search the bottom (Crick) strand of target sequences" help="in the sequence database"/>
+            <param name="toponly" truevalue="--toponly" falsevalue="" checked="False" type="boolean" 
+                label="Only search the top (Watson) strand of target sequences" help="in the sequence database"/>
+
+            <param name="cyk" truevalue="--cyk" falsevalue="" checked="False" type="boolean" 
+                label="Use the CYK algorithm, not Inside, to determine the final score of all hits" help=""/>
+            <param name="--acyk" truevalue="--cyk" falsevalue="" checked="False" type="boolean" 
+                label="Use the CYK algorithm to align hits" help="By default, the Durbin/Holmes optimal accuracy algorithm is used, which finds the alignment that maximizes the expected accuracy of all aligned residues."/>
+
+            <param name="notrunc" truevalue="--notrunc" falsevalue="" checked="False" type="boolean" 
+                label="Turn off truncated hit detection" help=""/>
+
+            <!-- accelleration pipeline -->
+
+            <param name="max" truevalue="--max" falsevalue="" checked="False" type="boolean" 
+                label="Turn off all filters, and run non-banded Inside on every full-length target sequence" help="This
+                increases sensitivity somewhat, at an extremely large cost in speed."/>
+
+            <param name="nohmm" truevalue="--nohmm" falsevalue="" checked="False" type="boolean" 
+                label="Turn off all HMM filter stages " help=""/>
+
+            <param name="mid" truevalue="--mid" falsevalue="" checked="False" type="boolean" 
+                label="Turn off the HMM SSV and Viterbi filter stages" help=""/>
+
+
+            <!-- Options for model-specific score thresholding -->
+            <!--
+            <param name="bitscore_thresholds" type="select" label="Bit score thresholds" help="Curated CM databases may define specific bit score thresholds for each CM, superseding any thresholding based on statistical significance alone.">
+                <option value="" selected="true">None</option>
+                <option value=" - -cut_ga">GA (gathering) bit scores</option>
+                <option value=" - -cut_nc">NC (noise cutoff) bit score</option>
+                <option value=" - -cut_tc">TC (trusted cutoff) bit score</option>
+            </param>
+            -->
+            <!-- Options for inclusion thresholds -->
+
+
+            <conditional name="inclusion_thresholds_opts">
+                <param name="inclusion_thresholds_selector" type="select" label="Inclusion thresholds"
+                help="Inclusion thresholds are stricter than reporting thresholds. Inclusion thresholds control which hits are considered to be reliable enough to be included in an output alignment or in a possible subsequent search round, or marked as significant (”!”) as opposed to questionable (”?”) in hit output.">
+                    <option value="" selected="true">default</option>
+                    <option value="--incE">Use E-value</option>
+                    <option value="--incT">Use bit score</option>
+                </param>
+                <when value=""/>
+                <when value="--incE">
+                    <param name="incE" type="float" value="0.01" size="5" label="Use E-value" help="of &lt;= X as the hit inclusion threshold.">
+                        <sanitizer>
+                            <valid initial="string.printable">
+                                <remove value="&apos;"/>
+                            </valid>
+                        </sanitizer>
+                    </param>
+                </when>
+                <when value="--incT">
+                    <param name="incT" type="integer" size="5" value="0" label="Use bit score" help="of >= X as the hit inclusion threshold.">
+                        <sanitizer>
+                            <valid initial="string.printable">
+                                <remove value="&apos;"/>
+                            </valid>
+                        </sanitizer>
+                    </param>
+                </when>
+            </conditional>
+
+            <!-- Options controlling reporting thresholds -->
+
+            <conditional name="reporting_thresholds_opts">
+                <param name="reporting_thresholds_selector" type="select" label="reporting thresholds"
+                help="Reporting thresholds control which hits are reported in output files">
+                    <option value="" selected="true">default</option>
+                    <option value="-E">Use E-value</option>
+                    <option value="-T">Use bit score</option>
+                </param>
+                <when value=""/>
+                <when value="-E">
+                    <param name="E" type="float" value="10.0" size="5" label="Use E-value" help="of &lt;= X as the hit reporting threshold. The default is 10.0, meaning that on average, about 10 false positives will be reported per query, so you can see the top of the noise and decide for yourself if it’s really noise.">
+                        <sanitizer>
+                            <valid initial="string.printable">
+                                <remove value="&apos;"/>
+                            </valid>
+                        </sanitizer>
+                    </param>
+                </when>
+                <when value="-T">
+                    <param name="T" type="integer" size="5" value="0" label="Use bit score" help="of >= X as the hit reporting threshold.">
+                        <sanitizer>
+                            <valid initial="string.printable">
+                                <remove value="&apos;"/>
+                            </valid>
+                        </sanitizer>
+                    </param>
+                </when>
+            </conditional>
+
+            <param name="A" truevalue="-A" falsevalue="" checked="False" type="boolean" 
+                label="Save a multiple alignment of all significant hits" help="... those satisfying inclusion thresholds"/>
+
+        </inputs>
+    <outputs>
+
+        <data format="tabular" name="outfile" label="cmsearch on ${on_string}"/>
+        <data format="tabular" name="multiple_alignment_output" label="cmsearch on ${on_string} (multi alignment)">
+            <filter>A is True</filter>
+        </data>
+
+    </outputs>
+    <help>
+
+
+**What it does**
+
+Infernal is used to search sequence databases for homologs of structural RNA sequences, and to make
+sequence- and structure-based RNA sequence alignments. Infernal needs a profile from a structurally
+annotated multiple sequence alignment of an RNA family with a position-specific scoring system for substitutions, 
+insertions, and deletions. Positions in the profile that are basepaired in the consensus secondary
+structure of the alignment are modeled as dependent on one another, allowing Infernal’s scoring system to
+consider the secondary structure, in addition to the primary sequence, of the family being modeled. Infernal
+profiles are probabilistic models called “covariance models”, a specialized type of stochastic context-free
+grammar (SCFG) (Lari and Young, 1990).
+
+Compared to other alignment and database search tools based only on sequence comparison, Infernal
+aims to be significantly more accurate and more able to detect remote homologs because it models sequence 
+and structure.
+
+
+Output format
+-------------
+
+(1) target name: The name of the target sequence or profile.
+(2) accession: The accession of the target sequence or profile, or ’-’ if none.
+(3) query name: The name of the query sequence or profile.
+(4) accession: The accession of the query sequence or profile, or ’-’ if none.
+(5) mdl (model): Which type of model was used to compute the final score. Either ’cm’ or ’hmm’. A CM is used to compute the final hit scores unless the model has zero basepairs or the --hmmonly option is used, in which case a HMM will be used.
+(6) mdl from (model coord): The start of the alignment of this hit with respect to the profile (CM or HMM), numbered 1..N for a profile of N consensus positions.
+(7) mdl to (model coord): The end of the alignment of this hit with respect to the profile (CM or HMM), numbered 1..N for a profile of N consensus positions.
+(8) seq from (ali coord): The start of the alignment of this hit with respect to the sequence, numbered 1..L for a sequence of L residues.
+(9) seq to (ali coord): The end of the alignment of this hit with respect to the sequence, numbered 1..L for a sequence of L residues.
+(10) strand: The strand on which the hit occurs on the sequence. ’+’ if the hit is on the top (Watson) strand, ’-’ if the hit is on the bottom (Crick) strand. If on the top strand, the “seq from” value will be less than or equal to the “seq to” value, else it will be greater than or equal to it. 
+(11) trunc: Indicates if this is predicted to be a truncated CM hit or not. This will be “no” if it is a CM hit that is not predicted to be truncated by the end of the sequence, “5’ ” or “3’ ” if the hit is predicted to have one or more 5’ or 3’ residues missing due to a artificial truncation of the sequence, or “5’&amp;3”’ if the hit is predicted to have one or more 5’ residues missing and one or more 3’ residues missing. If the hit is an HMM hit, this will always be ’-’.
+(12) pass: Indicates what “pass” of the pipeline the hit was detected on. This is probably only useful for testing and debugging. Non-truncated hits are found on the first pass, truncated hits are found on successive passes.
+(13) gc: Fraction of G and C nucleotides in the hit.
+(14) bias: The biased-composition correction: the bit score difference contributed by the null3 model for CM hits, or the null2 model for HMM hits. High bias scores may be a red flag for a false positive. It is difficult to correct for all possible ways in which a nonrandom but nonhomologous biological sequences can appear to be similar, such as short-period tandem repeats, so there are cases where the bias correction is not strong enough (creating false positives).
+(15) score: The score (in bits) for this target/query comparison. It includes the biased-composition cor-rection (the “null3” model for CM hits, or the “null2” model for HMM hits).
+(16) E-value: The expectation value (statistical significance) of the target. This is a per query E-value; i.e. calculated as the expected number of false positives achieving this comparison’s score for a single query against the search space Z. For cmsearch Z is defined as the total number of nucleotides in the target dataset multiplied by 2 because both strands are searched. For cmscan Z is the total number of nucleotides in the query sequence multiplied by 2 because both strands are searched and multiplied by the number of models in the target database. If you search with multiple queries and if you want to control the overall false positive rate of that search rather than the false positive rate per query, you will want to multiply this per-query E-value by how many queries you’re doing.
+(17) inc: Indicates whether or not this hit achieves the inclusion threshold: ’!’ if it does, ’?’ if it does not (and rather only achieves the reporting threshold). By default, the inclusion threshold is an E-value of 0.01 and the reporting threshold is an E-value of 10.0, but these can be changed with command line options as described in the manual pages.
+(18) description of target: The remainder of the line is the target’s description line, as free text.
+
+
+For further questions please refere to the Infernal Userguide_.
+
+.. _Userguide: http://selab.janelia.org/software/infernal/Userguide.pdf
+
+
+How do I cite Infernal?
+-----------------------
+
+The recommended citation for using Infernal 1.1 is E. P. Nawrocki and S. R. Eddy, Infernal 1.1: 100-fold faster RNA homology searches , Bioinformatics 29:2933-2935 (2013). 
+
+**Galaxy Wrapper Author**::
+
+    *  Bjoern Gruening, University of Freiburg
+
+    </help>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/cmstat.xml	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,101 @@
+<tool id="infernal_cmstat" name="Summary statistics" version="1.1.0.1">
+    <description>for covariance model (cmstat)</description>
+    <requirements>
+        <requirement type="package">infernal</requirement>
+        <requirement type="package" version="1.1">infernal</requirement>
+        <requirement type="package" version="8.21">gnu_coreutils</requirement>
+    </requirements>
+    <command>
+        ## a temp file is needed, because the standard tabular output from infernal is not usefull in Galaxy
+        ## it will be converted to a tab delimited file and piped to Galaxy
+        temp_tabular_output=\$(mktemp);
+
+        cmstat
+
+            #if str($cm_opts.cm_opts_selector) == "db":
+                $cm_opts.database.fields.path
+            #else:
+                $cm_opts.cmfile
+            #end if
+
+            > \$temp_tabular_output
+            ;
+
+            ## 1. replace all lines starting # (comment lines)
+            ## 2. replace the first 18 spaces with tabs, 18th field is a free text field (can contain spaces)
+            sed -e 's/#.*$//' -e '/^$/d' -e 's/ /\t/g' -e 's/\t/ /18g' \$temp_tabular_output > $outfile
+
+    </command>
+        <inputs>
+            <conditional name="cm_opts">
+                <param name="cm_opts_selector" type="select" label="Subject covariance models">
+                  <option value="db" selected="True">Locally installed covariance models</option>
+                  <option value="histdb">Covariance model from your history</option>
+                </param>
+                <when value="db">
+                    <param name="database" type="select" label="Covariance models">
+                        <options from_file="infernal.loc">
+                          <column name="value" index="0"/>
+                          <column name="name" index="1"/>
+                          <column name="path" index="2"/>
+                        </options>
+                    </param>
+                </when>
+                <when value="histdb">
+                    <param name="cmfile" type="data" format="cm" label="Covariance models file from the history."/>
+                </when>
+            </conditional>
+        </inputs>
+    <outputs>
+        <data format="tabular" name="outfile" label="cmstat on ${on_string}"/>
+    </outputs>
+    <help>
+
+
+**What it does**
+
+The cmstat utility prints out a tabular file of summary statistics for each given covariance model.
+
+
+Output format
+-------------
+
+By default, cmstat prints general statistics of the model and the alignment it was built from, one line per model in a
+tabular format. 
+
+The columns are:
+
+(1) The index of this profile, numbering each on in the file starting from 1.
+(2) The name of the profile.
+(3) The optional accession of the profile, or ”-” if there is none.
+(4) The number of sequences that the profile was estimated from.
+(5) The effective number of sequences that the profile was estimated from, after Infernal applied an effective sequence number calculation such as the default entropy weighting.
+(6) The length of the model in consensus residues (match states).
+(7) The expected maximum length of a hit to the model.
+(8) The number of basepairs in the model.
+(9) The number of bifurcations in the model.
+(10) What type of model will be used by default in cmsearch and cmscan for this profile, either ”cm” or ”hmm”. For profiles with 0 basepairs, this will be ”hmm” (unless the --nohmmonly option is used). For all other profiles, this will be ”cm”.
+(11) Mean relative entropy per match state, in bits. This is the expected (mean) score per con-
+     sensus position. This is what the default entropy-weighting method for effective sequence
+     number estimation focuses on, so for default Infernal, this value will often reflect the default
+     target for entropy-weighting. If the ”model” field for this profile is ”hmm”, this field will be ”-”.
+(12) Mean relative entropy per match state, in bits, if the CM were transformed into an HMM (information from structure is ignored). The larger the difference between the CM and HMM
+     relative entropy, the more the model will rely on structural conservation relative sequence conservation when identifying homologs.
+
+
+For further questions please refere to the Infernal Userguide_.
+
+.. _Userguide: http://selab.janelia.org/software/infernal/Userguide.pdf
+
+
+How do I cite Infernal?
+-----------------------
+
+The recommended citation for using Infernal 1.1 is E. P. Nawrocki and S. R. Eddy, Infernal 1.1: 100-fold faster RNA homology searches , Bioinformatics 29:2933-2935 (2013). 
+
+**Galaxy Wrapper Author**::
+
+    *  Bjoern Gruening, University of Freiburg
+
+    </help>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/datatypes_conf.xml	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,13 @@
+<?xml version="1.0"?>
+  <datatypes>
+   <datatype_files>
+     <datatype_file name="infernal.py"/>
+   </datatype_files>
+   <registration>
+        <!--INFERNAL1.1 Datatypes -->
+        <datatype extension="cm" type="galaxy.datatypes.infernal:Infernal_CM_1_1" display_in_upload="False" />
+   </registration>
+   <sniffers>
+        <sniffer type="galaxy.datatypes.infernal:Infernal_CM_1_1"/>
+   </sniffers>
+</datatypes>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/infernal.py	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,128 @@
+# -*- coding: utf-8 -*-
+
+from galaxy.datatypes.data import Text
+from galaxy.datatypes.sniff import get_headers, get_test_fname
+from galaxy.datatypes.data import get_file_peek
+import subprocess
+import os
+
+from galaxy.datatypes.metadata import MetadataElement
+from galaxy.datatypes import metadata
+
+def count_special_lines( word, filename, invert = False ):
+    """
+        searching for special 'words' using the grep tool
+        grep is used to speed up the searching and counting
+        The number of hits is returned.
+    """
+    try:
+        cmd = ["grep", "-c"]
+        if invert:
+            cmd.append('-v')
+        cmd.extend([word, filename])
+        out = subprocess.Popen(cmd, stdout=subprocess.PIPE)
+        return int(out.communicate()[0].split()[0])
+    except:
+        pass
+    return 0
+
+def count_lines( filename, non_empty = False):
+    """
+        counting the number of lines from the 'filename' file
+    """
+    try:
+        if non_empty:
+            out = subprocess.Popen(['grep', '-cve', '^\s*$', filename], stdout=subprocess.PIPE)
+        else:
+            out = subprocess.Popen(['wc', '-l', filename], stdout=subprocess.PIPE)
+        return int(out.communicate()[0].split()[0])
+    except:
+        pass
+    return 0
+
+
+class Infernal_CM_1_1( Text ):
+    file_ext = "cm"
+
+    MetadataElement( name="number_of_models", default=0, desc="Number of covariance models", readonly=True, visible=True, optional=True, no_value=0 )
+
+    def set_peek( self, dataset, is_multi_byte=False ):
+        if not dataset.dataset.purged:
+            dataset.peek = get_file_peek( dataset.file_name, is_multi_byte=is_multi_byte )
+            if (dataset.metadata.number_of_models == 1):
+                dataset.blurb = "1 model"
+            else:
+                dataset.blurb = "%s models" % dataset.metadata.number_of_models
+            dataset.peek = data.get_file_peek( dataset.file_name, is_multi_byte=is_multi_byte )
+        else:
+            dataset.peek = 'file does not exist'
+            dataset.blurb = 'file purged from disc'
+
+    def sniff( self, filename ):
+        if count_special_lines("^INFERNAL1/a", filename) > 0:
+            return True
+        else:
+            return False
+
+    def set_meta( self, dataset, **kwd ):
+        """
+        Set the number of models in dataset.
+        """
+        dataset.metadata.number_of_models = count_special_lines("^INFERNAL1/a", dataset.file_name)
+
+    def split( cls, input_datasets, subdir_generator_function, split_params):
+        """
+        Split the input files by model records.
+        """
+        if split_params is None:
+            return None
+
+        if len(input_datasets) > 1:
+            raise Exception("CM-file splitting does not support multiple files")
+        input_files = [ds.file_name for ds in input_datasets]
+
+        chunk_size = None
+        if split_params['split_mode'] == 'number_of_parts':
+            raise Exception('Split mode "%s" is currently not implemented for CM-files.' % split_params['split_mode'])
+        elif split_params['split_mode'] == 'to_size':
+            chunk_size = int(split_params['split_size'])
+        else:
+            raise Exception('Unsupported split mode %s' % split_params['split_mode'])
+
+        def _read_cm_records( filename ):
+            lines = []
+            with open(filename) as handle:
+                for line in handle:
+                    if line.startswith("INFERNAL1/a") and lines:
+                        yield lines
+                        lines = [line]
+                    else:
+                        lines.append( line )
+            yield lines
+
+        def _write_part_cm_file( accumulated_lines ):
+            part_dir = subdir_generator_function()
+            part_path = os.path.join( part_dir, os.path.basename( input_files[0] ) )
+            part_file = open( part_path, 'w' )
+            part_file.writelines( accumulated_lines )
+            part_file.close()
+
+        try:
+            cm_records = _read_cm_records( input_files[0] )
+            cm_lines_accumulated = []
+            for counter, cm_record in enumerate( cm_records, start = 1):
+                cm_lines_accumulated.extend( cm_record )
+                if counter % chunk_size == 0:
+                    _write_part_cm_file( cm_lines_accumulated )
+                    cm_lines_accumulated = []
+            if cm_lines_accumulated:
+                _write_part_cm_file( cm_lines_accumulated )
+        except Exception,  e:
+            log.error('Unable to split files: %s' % str(e))
+            raise
+    split = classmethod(split)
+
+if __name__ == '__main__':
+    Infernal_CM_1_1()
+    Stockholm_1_0()
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/readme.rst	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,67 @@
+================================================
+Galaxy wrapper for Infernal prediction tools
+================================================
+
+Infernal ("INFERence of RNA ALignment") is for searching DNA sequence databases 
+for RNA structure and sequence similarities. It is an implementation of a special 
+case of profile stochastic context-free grammars called covariance models (CMs). 
+A CM is like a sequence profile, but it scores a combination of sequence consensus 
+and RNA secondary structure consensus, so in many cases, it is more capable of 
+identifying RNA homologs that conserve their secondary structure more than their 
+primary sequence. 
+
+This wrapper is copyright 2013 by:
+ * Bjoern Gruening
+
+
+This prepository contains wrapper for the Infernal_ command line tool.
+
+.. _Infernal: http://infernal.janelia.org/
+
+
+The recommended citation for using Infernal 1.1 is E. P. Nawrocki and S. R. Eddy, Infernal 1.1: 100-fold faster RNA homology searches , Bioinformatics 29:2933-2935 (2013).
+
+
+============
+Installation
+============
+
+Please install Infernal and the tool wrappers with the Galaxy Tool Shed from
+
+http://toolshed.g2.bx.psu.edu/view/bgruening/infernal 
+
+
+=======
+History
+=======
+
+ - v1.1.0: Initial public release
+
+
+Bug Reports
+===========
+
+You can file an issue here https://github.com/bgruening/galaxytools/issues or ask
+us on the Galaxy development list http://lists.bx.psu.edu/listinfo/galaxy-dev
+
+
+Licence (MIT)
+=============
+
+Permission is hereby granted, free of charge, to any person obtaining a copy
+of this software and associated documentation files (the "Software"), to deal
+in the Software without restriction, including without limitation the rights
+to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
+copies of the Software, and to permit persons to whom the Software is
+furnished to do so, subject to the following conditions:
+
+The above copyright notice and this permission notice shall be included in
+all copies or substantial portions of the Software.
+
+THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
+THE SOFTWARE.
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/repository_dependencies.xml	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,4 @@
+<?xml version="1.0"?>
+<repositories description="This requires the datatype definitions for Multiple Sequence Alignment (MSA) formats (e.g. STOCKHOLM, SELEX, ClustalW).">
+    <repository changeset_revision="70227007b991" name="msa_datatypes" owner="iuc" toolshed="http://toolshed.g2.bx.psu.edu" />
+</repositories>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/infernal.loc.sample	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,17 @@
+#This is a sample file distributed with Galaxy that is used to define a
+#list of infernal covariance models, using three columns tab separated
+#(longer whitespace are TAB characters):
+#
+#The entries are as follows:
+#
+#<unique_id>	<covariance model name>	<path>
+#
+#Your infernal.loc file should include an entry per line for each "base name" 
+#you have stored.  For example:
+#
+#rfam01	RFAM 4.2 08 Aug 2013	/data/0/galaxy_data/infernal/08_08_2013/rfam.cm
+#
+#...etc...
+#
+
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Tue Apr 22 13:56:22 2014 -0400
@@ -0,0 +1,9 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="infernal" version="1.1">
+        <repository changeset_revision="b5c01b683dc9" name="package_infernal_1_1" owner="iuc" toolshed="http://toolshed.g2.bx.psu.edu" />
+    </package>
+    <package name="gnu_coreutils" version="8.21">
+        <repository changeset_revision="5f6c9b57f32b" name="package_gnu_coreutils_8_21" owner="iuc" toolshed="http://toolshed.g2.bx.psu.edu" />
+    </package>
+</tool_dependency>