# HG changeset patch # User bgruening # Date 1643460709 0 # Node ID fd7a16d073c5910a83e8c154d90c119a7952de51 # Parent 1c4cf4b7debe80e8c6c3d13e8ed5e40deb1c0a13 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/music/ commit 20f8561478535013e111d982b99639f48f1bea79" diff -r 1c4cf4b7debe -r fd7a16d073c5 macros.xml --- a/macros.xml Tue Nov 30 13:07:36 2021 +0000 +++ b/macros.xml Sat Jan 29 12:51:49 2022 +0000 @@ -1,9 +1,14 @@ - 1 + 2 0.1.1 + rdata + music-deconvolution diff -r 1c4cf4b7debe -r fd7a16d073c5 music-deconvolution.xml --- a/music-deconvolution.xml Tue Nov 30 13:07:36 2021 +0000 +++ b/music-deconvolution.xml Sat Jan 29 12:51:49 2022 +0000 @@ -1,5 +1,5 @@ + profile="21.09" license="GPL-3.0-or-later" > estimate cell type proportions in bulk RNA-seq data macros.xml @@ -25,20 +25,27 @@ bulk_eset = readRDS('$bulk_eset') scrna_eset = readRDS('$scrna_eset') +use_disease_factor = FALSE +maxyscale = NA #if str($do.method) == "estimateprops": +maxyscale = as.numeric('$do.maxyscale') ## yields "NA" if blank phenotype_factors = null_str_vec('$do.phenotype_factors') phenotype_factors_always_exclude = null_str_vec('$do.phenotype_factors_always_exclude') celltypes_label = null_str_vec('$do.celltypes_label') samples_label = null_str_vec('$do.samples_label') celltypes = null_str_vec('$do.celltypes') methods = c("MuSiC", "NNLS") -phenotype_target = null_str_vec('$do.phenotype_target') -phenotype_target_threshold = as.numeric('$do.phenotype_target_threshold') -sample_disease_group = null_str_vec('$do.sample_disease_group') -sample_disease_group_scale = as.integer('$do.sample_disease_group_scale') -compare_title = null_str_vec('$do.compare_title') + + #if str($do.disease_factor.use) == "yes": +use_disease_factor = TRUE +phenotype_scrna_target = null_str_vec('$do.disease_factor.phenotype_scrna_target') +phenotype_target = null_str_vec('$do.disease_factor.phenotype_target') +phenotype_target_threshold = as.numeric('$do.disease_factor.phenotype_target_threshold') +sample_disease_group = null_str_vec('$do.disease_factor.sample_disease_group') +sample_disease_group_scale = as.integer('$do.disease_factor.sample_disease_group_scale') + #end if outfile_pdf='$out_pdf' @@ -54,11 +61,11 @@ #if $i == 0: $repeat.cluster_id = list(cell.types = null_str_vec('$repeat.celltypes'), marker.names = null_str_vec('$repeat.marker_name'), - marker.list = read_list('$repeat.marker_list')) + marker.list = read_list('$repeat.marker_list')) #else , $repeat.cluster_id = list(cell.types = null_str_vec('$repeat.celltypes'), - marker.names = null_str_vec('$repeat.marker_name'), - marker.list = read_list('$repeat.marker_list')) + marker.names = null_str_vec('$repeat.marker_name'), + marker.list = read_list('$repeat.marker_list')) #end if #end for ) @@ -73,8 +80,8 @@ - - + + + + + + + + + + + + @@ -230,12 +261,15 @@ - - - - - - + + + + + + + + + @@ -257,10 +291,8 @@ Solid tissues often contain closely related cell types which leads to collinearity. To deal with collinearity, MuSiC employs a tree-guided procedure that recursively zooms in on closely related cell types. Briefly, we first group similar cell types into the same cluster and estimate cluster proportions, then recursively repeat this procedure within each cluster. -.. image:: $PATH_TO_IMAGES/FigureMethod.jpg - ]]> https://doi.org/10.1038/s41467-018-08023-x - + \ No newline at end of file diff -r 1c4cf4b7debe -r fd7a16d073c5 scripts/dendrogram.R --- a/scripts/dendrogram.R Tue Nov 30 13:07:36 2021 +0000 +++ b/scripts/dendrogram.R Sat Jan 29 12:51:49 2022 +0000 @@ -10,7 +10,7 @@ if (lfile == "None") { return(NULL) } - return(read.table(file = lfile, header = FALSE, + return(read.table(file = lfile, header = FALSE, check.names = FALSE, stringsAsFactors = FALSE)$V1) } diff -r 1c4cf4b7debe -r fd7a16d073c5 scripts/estimateprops.R --- a/scripts/estimateprops.R Tue Nov 30 13:07:36 2021 +0000 +++ b/scripts/estimateprops.R Sat Jan 29 12:51:49 2022 +0000 @@ -17,13 +17,21 @@ estimated_music_props <- est_prop$Est.prop.weighted estimated_nnls_props <- est_prop$Est.prop.allgene +scale_yaxes <- function(gplot, value) { + if (is.na(value)) { + gplot + } else { + gplot + scale_y_continuous(lim = c(0, value)) + } +} + ## Show different in estimation methods ## Jitter plot of estimated cell type proportions -jitter_fig <- Jitter_Est( +jitter_fig <- scale_yaxes(Jitter_Est( list(data.matrix(estimated_music_props), data.matrix(estimated_nnls_props)), method.name = methods, title = "Jitter plot of Est Proportions", - size = 2, alpha = 0.7) + theme_minimal() + size = 2, alpha = 0.7) + theme_minimal(), maxyscale) ## Make a Plot @@ -42,11 +50,6 @@ message(celltypes) } -if (phenotype_target_threshold == -99) { - phenotype_target_threshold <- -Inf - message("phenotype target threshold set to -Inf") -} - if (is.null(phenotype_factors)) { phenotype_factors <- colnames(pData(bulk_eset)) } @@ -54,67 +57,94 @@ phenotype_factors <- phenotype_factors[ !(phenotype_factors %in% phenotype_factors_always_exclude)] message("Phenotype Factors to use:") -message(phenotype_factors) - +message(paste0(phenotype_factors, collapse = ", ")) m_prop$CellType <- factor(m_prop$CellType, levels = celltypes) # nolint m_prop$Method <- factor(rep(methods, each = nrow(estimated_music_props_flat)), # nolint levels = methods) -m_prop$Disease_factor <- rep(bulk_eset[[phenotype_target]], 2 * length(celltypes)) # nolint -m_prop <- m_prop[!is.na(m_prop$Disease_factor), ] -## Generate a TRUE/FALSE table of Normal == 1 and Disease == 2 -sample_groups <- c("Normal", sample_disease_group) -m_prop$Disease <- factor(sample_groups[(m_prop$Disease_factor > phenotype_target_threshold) + 1], # nolint - levels = sample_groups) + +if (use_disease_factor) { + + if (phenotype_target_threshold == -99) { + phenotype_target_threshold <- -Inf + message("phenotype target threshold set to -Inf") + } + + m_prop$Disease_factor <- rep(bulk_eset[[phenotype_target]], 2 * length(celltypes)) # nolint + m_prop <- m_prop[!is.na(m_prop$Disease_factor), ] + ## Generate a TRUE/FALSE table of Normal == 1 and Disease == 2 + sample_groups <- c("Normal", sample_disease_group) + m_prop$Disease <- factor(sample_groups[(m_prop$Disease_factor > phenotype_target_threshold) + 1], # nolint + levels = sample_groups) -## Binary to scale: e.g. TRUE / 5 = 0.2 -m_prop$D <- (m_prop$Disease == # nolint - sample_disease_group) / sample_disease_group_scale -## NA's are not included in the comparison below -m_prop <- rbind(subset(m_prop, Disease != sample_disease_group), - subset(m_prop, Disease == sample_disease_group)) + ## Binary to scale: e.g. TRUE / 5 = 0.2 + m_prop$D <- (m_prop$Disease == # nolint + sample_disease_group) / sample_disease_group_scale + ## NA's are not included in the comparison below + m_prop <- rbind(subset(m_prop, Disease != sample_disease_group), + subset(m_prop, Disease == sample_disease_group)) -jitter_new <- ggplot(m_prop, aes(Method, Prop)) + - geom_point(aes(fill = Method, color = Disease, stroke = D, shape = Disease), - size = 2, alpha = 0.7, - position = position_jitter(width = 0.25, height = 0)) + - facet_wrap(~ CellType, scales = "free") + - scale_colour_manual(values = c("white", "gray20")) + - scale_shape_manual(values = c(21, 24)) + theme_minimal() + jitter_new <- scale_yaxes(ggplot(m_prop, aes(Method, Prop)) + + geom_point(aes(fill = Method, color = Disease, stroke = D, shape = Disease), + size = 2, alpha = 0.7, + position = position_jitter(width = 0.25, height = 0)) + + facet_wrap(~ CellType, scales = "free") + + scale_colour_manual(values = c("white", "gray20")) + + scale_shape_manual(values = c(21, 24)) + theme_minimal(), maxyscale) + +} + +if (use_disease_factor) { -## Plot to compare method effectiveness -## Create dataframe for beta cell proportions and Disease_factor levels -m_prop_ana <- data.frame(pData(bulk_eset)[rep(1:nrow(estimated_music_props), 2), #nolint - phenotype_factors], - ct.prop = c(estimated_music_props[, 2], - estimated_nnls_props[, 2]), - Method = factor(rep(methods, - each = nrow(estimated_music_props)), - levels = methods)) -colnames(m_prop_ana)[1:length(phenotype_factors)] <- phenotype_factors #nolint -m_prop_ana <- subset(m_prop_ana, !is.na(m_prop_ana[phenotype_target])) -m_prop_ana$Disease <- factor(sample_groups[( # nolint - m_prop_ana[phenotype_target] > phenotype_target_threshold) + 1], - sample_groups) -m_prop_ana$D <- (m_prop_ana$Disease == # nolint - sample_disease_group) / sample_disease_group_scale + ## Plot to compare method effectiveness + ## Create dataframe for beta cell proportions and Disease_factor levels + ## - Ugly code. Essentially, doubles the cell type proportions for each + ## set of MuSiC and NNLS methods + m_prop_ana <- data.frame(pData(bulk_eset)[rep(1:nrow(estimated_music_props), 2), #nolint + phenotype_factors], + ## get proportions of target cell type + ct.prop = c(estimated_music_props[, phenotype_scrna_target], + estimated_nnls_props[, phenotype_scrna_target]), + ## + Method = factor(rep(methods, + each = nrow(estimated_music_props)), + levels = methods)) + ## - fix headers + colnames(m_prop_ana)[1:length(phenotype_factors)] <- phenotype_factors #nolint + ## - drop NA for target phenotype (e.g. hba1c) + m_prop_ana <- subset(m_prop_ana, !is.na(m_prop_ana[phenotype_target])) + m_prop_ana$Disease <- factor( # nolint + ## - Here we set Normal/Disease assignments across the two MuSiC and NNLS methods + sample_groups[( + m_prop_ana[phenotype_target] > phenotype_target_threshold) + 1 + ], + sample_groups) + ## - Then we scale this binary assignment to a plotable factor + m_prop_ana$D <- (m_prop_ana$Disease == # nolint + sample_disease_group) / sample_disease_group_scale -jitt_compare <- ggplot(m_prop_ana, aes_string(phenotype_target, "ct.prop")) + - geom_smooth(method = "lm", se = FALSE, col = "black", lwd = 0.25) + - geom_point(aes(fill = Method, color = Disease, stroke = D, shape = Disease), - size = 2, alpha = 0.7) + facet_wrap(~ Method) + - ggtitle(compare_title) + theme_minimal() + - scale_colour_manual(values = c("white", "gray20")) + - scale_shape_manual(values = c(21, 24)) + jitt_compare <- scale_yaxes(ggplot(m_prop_ana, aes_string(phenotype_target, "ct.prop")) + + geom_smooth(method = "lm", se = FALSE, col = "black", lwd = 0.25) + + geom_point(aes(fill = Method, color = Disease, stroke = D, shape = Disease), + size = 2, alpha = 0.7) + facet_wrap(~ Method) + + ggtitle(paste0(toupper(phenotype_target), " vs. ", + toupper(phenotype_scrna_target), " Cell Type Proportion")) + + theme_minimal() + + ylab(paste0("Proportion of ", + phenotype_scrna_target, " cells")) + + xlab(paste0("Level of bulk factor (", phenotype_target, ")")) + + scale_colour_manual(values = c("white", "gray20")) + + scale_shape_manual(values = c(21, 24)), maxyscale) +} ## BoxPlot -plot_box <- Boxplot_Est(list( +plot_box <- scale_yaxes(Boxplot_Est(list( data.matrix(estimated_music_props), data.matrix(estimated_nnls_props)), method.name = c("MuSiC", "NNLS")) + theme(axis.text.x = element_text(angle = -90), axis.text.y = element_text(size = 8)) + - ggtitle(element_blank()) + theme_minimal() + ggtitle(element_blank()) + theme_minimal(), maxyscale) ## Heatmap plot_hmap <- Prop_heat_Est(list( @@ -125,8 +155,15 @@ axis.text.y = element_text(size = 6)) pdf(file = outfile_pdf, width = 8, height = 8) -plot_grid(jitter_fig, plot_box, labels = "auto", ncol = 1, nrow = 2) -plot_grid(jitter_new, jitt_compare, labels = "auto", ncol = 1, nrow = 2) +if (length(celltypes) <= 8) { + plot_grid(jitter_fig, plot_box, labels = "auto", ncol = 1, nrow = 2) +} else { + print(jitter_fig) + plot_box +} +if (use_disease_factor) { + plot_grid(jitter_new, jitt_compare, labels = "auto", ncol = 1, nrow = 2) +} plot_hmap message(dev.off()) @@ -159,29 +196,32 @@ quote = F, sep = "\t", col.names = NA) -## Summary table -for (meth in methods) { - ##lm_beta_meth = lm(ct.prop ~ age + bmi + hba1c + gender, data = - sub_data <- subset(m_prop_ana, Method == meth) - ## We can only do regression where there are more than 1 factors - ## so we must find and exclude the ones which are not - gt1_facts <- sapply(phenotype_factors, function(facname) { - return(length(unique(sort(sub_data[[facname]]))) == 1) - }) - form_factors <- phenotype_factors - exclude_facts <- names(gt1_facts)[gt1_facts] - if (length(exclude_facts) > 0) { - message("Factors with only one level will be excluded:") - message(exclude_facts) - form_factors <- phenotype_factors[ - !(phenotype_factors %in% exclude_facts)] +if (use_disease_factor) { + ## Summary table of linear regressions of disease factors + for (meth in methods) { + ##lm_beta_meth = lm(ct.prop ~ age + bmi + hba1c + gender, data = + sub_data <- subset(m_prop_ana, Method == meth) + + ## We can only do regression where there are more than 1 factors + ## so we must find and exclude the ones which are not + gt1_facts <- sapply(phenotype_factors, function(facname) { + return(length(unique(sort(sub_data[[facname]]))) == 1) + }) + form_factors <- phenotype_factors + exclude_facts <- names(gt1_facts)[gt1_facts] + if (length(exclude_facts) > 0) { + message("Factors with only one level will be excluded:") + message(exclude_facts) + form_factors <- phenotype_factors[ + !(phenotype_factors %in% exclude_facts)] + } + lm_beta_meth <- lm(as.formula( + paste("ct.prop", paste(form_factors, collapse = " + "), + sep = " ~ ")), data = sub_data) + message(paste0("Summary: ", meth)) + capture.output(summary(lm_beta_meth), + file = paste0("report_data/summ_Log of ", + meth, + " fitting.txt")) } - lm_beta_meth <- lm(as.formula( - paste("ct.prop", paste(form_factors, collapse = " + "), - sep = " ~ ")), data = sub_data) - message(paste0("Summary: ", meth)) - capture.output(summary(lm_beta_meth), - file = paste0("report_data/summ_Log of ", - meth, - " fitting.txt")) } diff -r 1c4cf4b7debe -r fd7a16d073c5 scripts/inspect.R --- a/scripts/inspect.R Tue Nov 30 13:07:36 2021 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,27 +0,0 @@ - -suppressWarnings(suppressPackageStartupMessages(library(xbioc))) -suppressWarnings(suppressPackageStartupMessages(library(MuSiC))) - -args <- commandArgs(trailingOnly = TRUE) -source(args[1]) - -printout <- function(text) { - if (typeof(text) %in% c("list", "vector", "integer", "double", "numeric")) { - write.table(text, file = outfile_tab, quote = F, sep = "\t", - col.names = NA) - } else { - ## text - print(typeof(text)) - capture.output(text, file = outfile_tab) # nolint - } -} - -if (inspector %in% c("print", "pData", "fData", "dims", - "experimentData", "protocolData", "exprs", - "signature", "annotation", "abstract")) { - op <- get(inspector) - tab <- op(rds_eset) - printout(tab) -} else { - stop(paste0("No such option:", inspector)) -} diff -r 1c4cf4b7debe -r fd7a16d073c5 test-data/default_output_no_disease.pdf Binary file test-data/default_output_no_disease.pdf has changed diff -r 1c4cf4b7debe -r fd7a16d073c5 test-data/mouse_scrna_exprs.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/mouse_scrna_exprs.tabular Sat Jan 29 12:51:49 2022 +0000 @@ -0,0 +1,101 @@ + TGGTTCCGTCGGCTCA-2 CGAGCCAAGCGTCAAG-4 GAATGAAGTTTGGGCC-5 CTCGTACGTTGCCTCT-7 TTCTCAATCCACGCAG-5 CCTTCCCCATACCATG-4 ACTTTCACAGCTGGCT-7 TGGGAAGCAAAGTGCG-7 TCTATTGAGTAGGCCA-7 TCGGTAACATCACGTA-2 GGGTTGCCAGCTGTAT-2 TGCGGGTGTCATATCG-6 ACTTGTTTCATATCGG-5 CCAATCCCACGGCGTT-2 CTAAGACCACCAGGCT-7 TTAACTCAGTAGGCCA-6 GTACTCCGTAACGCGA-1 GCCTCTAGTTGTACAC-2 TTCGAAGTCCTGCAGG-3 TTCTACAAGTTGTAGA-7 CCGTTCAGTTGAACTC-7 GTGTTAGTCAGCTCGG-1 GGATTACGTGTGCGTC-6 TACGGTATCCGTTGTC-6 TTAGTTCGTATTAGCC-5 CCCAATCGTAGCGATG-3 ACACCAATCTGCGTAA-7 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0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 diff -r 1c4cf4b7debe -r fd7a16d073c5 test-data/mouse_scrna_pheno.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/mouse_scrna_pheno.tabular Sat Jan 29 12:51:49 2022 +0000 @@ -0,0 +1,101 @@ + sampleID SubjectName cellTypeID cellType +TGGTTCCGTCGGCTCA-2 2 Mouse2 3 PT +CGAGCCAAGCGTCAAG-4 4 Mouse4 5 DCT +GAATGAAGTTTGGGCC-5 5 Mouse5 3 PT +CTCGTACGTTGCCTCT-7 7 Mouse7 3 PT +TTCTCAATCCACGCAG-5 5 Mouse5 4 LOH +CCTTCCCCATACCATG-4 4 Mouse4 14 T lymph +ACTTTCACAGCTGGCT-7 7 Mouse7 3 PT +TGGGAAGCAAAGTGCG-7 7 Mouse7 3 PT +TCTATTGAGTAGGCCA-7 7 Mouse7 3 PT +TCGGTAACATCACGTA-2 2 Mouse2 3 PT +GGGTTGCCAGCTGTAT-2 2 Mouse2 2 Podo +TGCGGGTGTCATATCG-6 6 Mouse6 3 PT +ACTTGTTTCATATCGG-5 5 Mouse5 14 T lymph +CCAATCCCACGGCGTT-2 2 Mouse2 5 DCT +CTAAGACCACCAGGCT-7 7 Mouse7 3 PT +TTAACTCAGTAGGCCA-6 6 Mouse6 3 PT +GTACTCCGTAACGCGA-1 1 Mouse1 3 PT +GCCTCTAGTTGTACAC-2 2 Mouse2 3 PT +TTCGAAGTCCTGCAGG-3 3 Mouse3 3 PT +TTCTACAAGTTGTAGA-7 7 Mouse7 3 PT +CCGTTCAGTTGAACTC-7 7 Mouse7 7 CD-IC +GTGTTAGTCAGCTCGG-1 1 Mouse1 4 LOH +GGATTACGTGTGCGTC-6 6 Mouse6 3 PT +TACGGTATCCGTTGTC-6 6 Mouse6 3 PT +TTAGTTCGTATTAGCC-5 5 Mouse5 3 PT +CCCAATCGTAGCGATG-3 3 Mouse3 3 PT +ACACCAATCTGCGTAA-7 7 Mouse7 5 DCT +AATCCAGTCCAAACTG-7 7 Mouse7 5 DCT +CAGAATCAGCAATATG-1 1 Mouse1 3 PT +GCACATAAGCCGGTAA-5 5 Mouse5 5 DCT +CCTTCCCAGGAGTTTA-5 5 Mouse5 3 PT +CGGAGCTAGGACTGGT-5 5 Mouse5 4 LOH +TACGGATGTAAATGTG-4 4 Mouse4 3 PT +GGCAATTCATTCACTT-2 2 Mouse2 3 PT +CTCGGGAGTCTGCGGT-4 4 Mouse4 6 CD-PC +CATTCGCGTCCTCTTG-2 2 Mouse2 8 CD-Trans +CGCGTTTAGATCGATA-1 1 Mouse1 6 CD-PC +GGGTTGCCACCAACCG-4 4 Mouse4 7 CD-IC +TGTGTTTCATCGATGT-2 2 Mouse2 3 PT +AGAGTGGAGCTGTTCA-7 7 Mouse7 3 PT +CTCACACGTCTCACCT-3 3 Mouse3 3 PT +AGTTGGTTCCACGAAT-7 7 Mouse7 3 PT +ATCTGCCAGACCACGA-6 6 Mouse6 3 PT +TGTATTCCATTGAGCT-7 7 Mouse7 3 PT +TGAAAGAGTAGCCTAT-7 7 Mouse7 3 PT +AAATGCCAGAACTGTA-7 7 Mouse7 5 DCT +TTTGCGCTCTACCAGA-4 4 Mouse4 3 PT +ACATACGGTTTCCACC-6 6 Mouse6 3 PT +GCCTCTAGTTCCACAA-7 7 Mouse7 3 PT +GGGAGATGTACTCTCC-6 6 Mouse6 1 Endo +GAACGGATCTTGTACT-7 7 Mouse7 3 PT +TACCTTATCCTAGAAC-1 1 Mouse1 3 PT +GCGCGATAGATGCCAG-2 2 Mouse2 3 PT +GACAGAGCAAGTTGTC-7 7 Mouse7 3 PT +TGACTAGGTATGAATG-3 3 Mouse3 4 LOH +CACACTCAGTCACGCC-6 6 Mouse6 3 PT +ATTGGTGGTTAGGGTG-5 5 Mouse5 3 PT +AGCAGCCCAGCGTAAG-2 2 Mouse2 1 Endo +CATTCGCAGCCTTGAT-6 6 Mouse6 3 PT +GCGAGAACATAGACTC-2 2 Mouse2 14 T lymph +AGTCTTTGTAATAGCA-7 7 Mouse7 3 PT +TCGCGAGCAGACACTT-7 7 Mouse7 3 PT +CGGAGTCCAGCAGTTT-2 2 Mouse2 3 PT +GGTGTTACACACATGT-7 7 Mouse7 3 PT +TTCTCAAGTAAGTGTA-2 2 Mouse2 1 Endo +TGCTGCTAGTCAATAG-2 2 Mouse2 3 PT +GATGAGGTCTACCAGA-2 2 Mouse2 3 PT +ACATACGGTTGTACAC-5 5 Mouse5 3 PT +ACGAGGACAGCTATTG-7 7 Mouse7 4 LOH +CGATGTATCGGCGGTT-2 2 Mouse2 3 PT +CTGCGGATCACAACGT-2 2 Mouse2 13 B lymph +CGAACATAGTTGAGTA-5 5 Mouse5 3 PT +TAGTTGGTCGCGATCG-6 6 Mouse6 5 DCT +GCAGCCACAATGTAAG-4 4 Mouse4 1 Endo +CTCACACCAATAACGA-7 7 Mouse7 3 PT +CCTTTCTCATGAAGTA-2 2 Mouse2 7 CD-IC +AGTGTCAAGAGCAATT-7 7 Mouse7 3 PT +AGCATACGTAAAGGAG-1 1 Mouse1 6 CD-PC +ACACCAATCTCGCTTG-5 5 Mouse5 3 PT +GGGATGAGTATCAGTC-6 6 Mouse6 3 PT +TGACAACAGAAGCCCA-2 2 Mouse2 3 PT +CGAATGTTCACAATGC-1 1 Mouse1 1 Endo +GCACTCTTCCGCATAA-1 1 Mouse1 3 PT +CACCAGGTCCCAAGAT-2 2 Mouse2 3 PT +GTTACAGCACCGCTAG-6 6 Mouse6 3 PT +TAGCCGGCAGTACACT-2 2 Mouse2 11 Macro +ACGATGTGTTAAAGAC-2 2 Mouse2 9 Novel1 +CCTTCCCAGTCTCGGC-7 7 Mouse7 3 PT +TAGTGGTTCTCTGTCG-7 7 Mouse7 7 CD-IC +TAGCCGGAGGCTAGCA-5 5 Mouse5 7 CD-IC +TTGTAGGTCAGCACAT-1 1 Mouse1 4 LOH +GAATAAGCAGCTTCGG-7 7 Mouse7 3 PT +TCGCGAGAGTCCGGTC-3 3 Mouse3 14 T lymph +TCAACGAAGAGTAAGG-2 2 Mouse2 5 DCT +CAGGTGCCACGAAATA-5 5 Mouse5 3 PT +TGTGTTTCACTATCTT-2 2 Mouse2 3 PT +TGGCCAGAGTGAAGAG-6 6 Mouse6 3 PT +ACCAGTAAGTAGCCGA-2 2 Mouse2 6 CD-PC +GCGGGTTAGAAGGTTT-1 1 Mouse1 3 PT +CAGTCCTGTCATTAGC-2 2 Mouse2 7 CD-IC