# HG changeset patch
# User bgruening
# Date 1528235771 14400
# Node ID 709490665bad75d5250906267d184ea48300c725
# Parent  f9dadc36219711b1d82a36ccf1ee71858ec4ebcb
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/nanopolish commit d3227eb74ad38fac307911b60c8a20a13349bcf9

diff -r f9dadc362197 -r 709490665bad macros.xml
--- a/macros.xml	Wed May 30 11:55:55 2018 -0400
+++ b/macros.xml	Tue Jun 05 17:56:11 2018 -0400
@@ -1,7 +1,7 @@
 <macros>
     <xml name="requirements">
         <requirements>
-        <requirement type="package" version="0.9.0">nanopolish</requirement>
+        <requirement type="package" version="0.9.2">nanopolish</requirement>
             <yield/>
         </requirements>
     </xml>
@@ -33,4 +33,4 @@
             <yield />
         </citations>
     </xml>
-</macros>
\ No newline at end of file
+</macros>
diff -r f9dadc362197 -r 709490665bad nanopolish_methylation.xml
--- a/nanopolish_methylation.xml	Wed May 30 11:55:55 2018 -0400
+++ b/nanopolish_methylation.xml	Tue Jun 05 17:56:11 2018 -0400
@@ -17,12 +17,20 @@
         nanopolish index -d fast5_files/ reads.fasta &&
         ln -s '$b' reads.bam &&
         ln -s '${b.metadata.bam_index}' reads.bam.bai &&
-        ln -s '$g' genome.fa &&
+        #if $reference_source.reference_source_selector == 'history':
+            ln -f -s '$reference_source.ref_file' genome.fa &&
+        #else:
+            ln -f -s '$reference_source.ref_file.fields.path' genome.fa &&
+        #end if
         
         nanopolish call-methylation
         -r reads.fasta
         -b reads.bam
         -g genome.fa
+        #if str($batchsize):
+            -K $batchsize
+        #end if
+        --threads "\${GALAXY_SLOTS:-4}"        
         #if $w and str($w).strip():
           -w "${w}"
         #end if  
@@ -35,10 +43,25 @@
 
         <!-- variants consensus inputs -->
         <param type="data" argument="-b" format="bam" label="Reads aligned to the reference genome" />
-        <param type="data" argument="-g" format="fasta" label="The reference genome"/>
-
+        <conditional name="reference_source">
+          <param name="reference_source_selector" type="select" label="Load reference genome from">
+            <option value="cached">Local cache</option>
+            <option value="history">History</option>
+          </param>
+          <when value="cached">
+            <param name="ref_file" type="select" label="Using reference genome" help="REFERENCE_SEQUENCE">
+              <options from_data_table="all_fasta">
+              </options>
+              <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+            </param>
+          </when>
+          <when value="history">
+            <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="REFERENCE_SEQUENCE; You can upload a FASTA sequence to the history and use it as reference" />
+          </when>
+        </conditional>
         <param argument="-w" type="text" optional="true"
             label="find variants in window of region chromsome:start-end" />
+        <param name="batchsize" type="integer" optional="true" value="" label="Batch size" help="(-K)"/>
 
     </inputs>
 
@@ -48,18 +71,26 @@
     </outputs>
     <tests>
         <test>
-      <!-- index test -->
             <param name="input_merged" ftype="fasta" value="reads.fasta" />
             <param name="input_reads_raw" ftype="fast5.tar.gz" value="fast5_files.tar.gz" />
-            
-      <!-- variants consensus test -->
             <param name="b" value="reads.sorted.bam" />
-            <param name="g" value="draft.fa" />
+            <param name="reference_source_selector" value="history" />
+            <param name="ref_file" value="draft.fa" />
             <param name="w" value="tig00000001:200000-202000" />
-            
+            <param name="batchsize" value="512" />
             <output name="output_methylation_calls" file="methylation_calls.tsv" />
         </test>
-    </tests>
+         <test>
+            <param name="input_merged" ftype="fasta" value="reads.fasta" />
+            <param name="input_reads_raw" ftype="fast5.tar.gz" value="fast5_files.tar.gz" />
+            <param name="reference_source_selector" value="cached" />
+            <param name="ref_file" value="draft"/>
+            <param name="b" value="reads.sorted.bam" />
+            <param name="w" value="tig00000001:200000-202000" />
+            <param name="batchsize" value="512" />
+            <output name="output_methylation_calls" file="methylation_calls.tsv" />
+        </test>
+    </tests>    
     <help><![CDATA[
         Usage: nanopolish call-methylation [OPTIONS] --reads reads.fa --bam alignments.bam --genome genome.fa
         Classify nucleotides as methylated or not.
diff -r f9dadc362197 -r 709490665bad test-data/all_fasta.loc
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/all_fasta.loc	Tue Jun 05 17:56:11 2018 -0400
@@ -0,0 +1,1 @@
+draft	draft	draft	${__HERE__}/draft.fa
\ No newline at end of file
diff -r f9dadc362197 -r 709490665bad tool_data_table_conf.xml.sample
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.sample	Tue Jun 05 17:56:11 2018 -0400
@@ -0,0 +1,9 @@
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc-->
+<tables>
+    <!-- Locations of all fasta files under genome directory -->
+    <table name="all_fasta" comment_char="#" allow_duplicate_entries="False">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/all_fasta.loc" />
+    </table>
+</tables>
+
diff -r f9dadc362197 -r 709490665bad tool_data_table_conf.xml.test
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.test	Tue Jun 05 17:56:11 2018 -0400
@@ -0,0 +1,7 @@
+<tables>
+    <!-- Locations of all fasta files under genome directory -->
+    <table name="all_fasta" comment_char="#" allow_duplicate_entries="False">
+        <columns>value, dbkey, name, path</columns>
+        <file path="${__HERE__}/test-data/all_fasta.loc" />
+    </table>
+</tables>