Mercurial > repos > bgruening > reago
diff reago.xml @ 0:13dd84cab09e draft default tip
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/reago commit f5be73e67192909db96dfab214f4360eb41757a4
author | bgruening |
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date | Sat, 27 Apr 2024 17:59:57 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/reago.xml Sat Apr 27 17:59:57 2024 +0000 @@ -0,0 +1,130 @@ +<tool id="reago" name="Reago" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="22.05"> + <description> to assemble rRNA</description> + <macros> + <token name="@TOOL_VERSION@">1.1</token> + <token name="@VERSION_SUFFIX@">1</token> + </macros> + <xrefs> + <xref type="bio.tools">reago</xref> + </xrefs> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">reago</requirement> + <requirement type="package" version="1.11">networkx</requirement> + </requirements> + <command detect_errors="exit_code"> + <![CDATA[ + #set $r1_output=$os.path.splitext($os.path.basename(str($r1_file)))[0] + #set $r2_output=$os.path.splitext($os.path.basename(str($r2_file)))[0] + + python '$__tool_directory__/format_reago_input_files.py' + --r1_input '$r1_file' + --r2_input '$r2_file' + --r1_output '$r1_output' + --r2_output '$r2_output' + + && + filter_input.py + '$r1_output' + '$r2_output' + . + \$(dirname \$(which reago.py))/cm/ + $cm_to_use + "\${GALAXY_SLOTS:-4}" + + && + reago.py + 'filtered.fasta' + . + -l $read_length + -o $overlap + -t $tip_size + -b $path_finding_parameter +]]> + </command> + + <inputs> + <param name="r1_file" type="data" format="fasta" label="R1 input sequence + file" help=""/> + + <param name="r2_file" type="data" format="fasta" label="R2 input sequence + file" help=""/> + + <param name="read_length" type="integer" min="1" max="1000" value="101" + label="Read length" help="(-l)"/> + + <param name="overlap" type="float" min="0" max="1" value="0.8" + label="Overlap" help="(-o)"/> + + <param name="tip_size" type="integer" min="0" max="1000" value="30" + label="Tip size" help="(-t)"/> + + <param name="path_finding_parameter" type="integer" min="2" max="11" + value="10" label="Path finding parameter" help="(-b)"/> + + <param name="cm_to_use" type="select" label="Database to use to to identify 16S reads" help=""> + <option value="b">Bacteria only</option> + <option value="a">Archea only</option> + <option value="ab">Bacteria and archea</option> + </param> + </inputs> + <outputs> + <data format="fasta" name="full_genes" + from_work_dir="full_genes.fasta" + label="Full genes of ${on_string} (Framebot)" /> + <data format="fasta" name="fragments" + from_work_dir="fragments.fasta" + label="Fragment genes of ${on_string} (Framebot)" /> + </outputs> + <tests> + <test> + <param name="r1_file" value="reago_sample_1.fasta"/> + <param name="r2_file" value="reago_sample_2.fasta"/> + <param name="read_length" value="101" /> + <param name="overlap" value="0.8" /> + <param name="tip_size" value="30" /> + <param name="path_finding_parameter" value="10"/> + <param name="cm_to_use" value="ab" /> + <output name="full_genes" file="reago_full_genes.fasta"/> + <output name="fragments" file="reago_fragments.fasta"/> + </test> + </tests> + <help> +<![CDATA[ + +**What it does** + +Reago is tool to assembly 16S ribosomal RNA recovery from metagenomic data. +More information on `Github repository <https://github.com/chengyuan/reago-1.1>`_. + +----- + +**Input** + +Sequence files with r1 and r2 are required. + +----- + +**Parameters** + +The parameters are + + - The overlap percentage + - The threshold for error correction + - The tip size + - The parameter for path finding + +----- + +**Output** + +Reago produces two output files: + + - A sequence file with full genes + - A sequence file with fragment genes + + ]]> + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btv231</citation> + </citations> +</tool>