SUMMARISING RUN PARAMETERS ========================== Input filename: ./input_singles Trimming mode: single-end Trim Galore version: 0.3.7 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length before a sequence gets removed: 20 bp cutadapt version 1.1 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ./input_singles Maximum error rate: 10.00% Processed reads: 2 Trimmed reads: 1 ( 50.0%) Total basepairs: 168 (0.0 Mbp) Trimmed basepairs: 1 (0.0 Mbp) (0.60% of total) Too short reads: 0 ( 0.0% of processed reads) Too long reads: 0 ( 0.0% of processed reads) Total time: 0.00 s Time per read: 0.32 ms === Adapter 1 === Adapter 'AGATCGGAAGAGC', length 13, was trimmed 1 times. Lengths of removed sequences length count expected 1 1 0.5 RUN STATISTICS FOR INPUT FILE: ./input_singles ============================================= 2 sequences processed in total Sequences removed because they became shorter than the length cutoff of 20 bp: 0 (0.0%)