# HG changeset patch
# User big-tiandm
# Date 1406790554 14400
# Node ID 45d9b0f6a0d6117a6b0241972dde9a43c3425c82
# Parent  9ae0d25e41699e20a714b6e5e4ca36d3512796c0
Uploaded

diff -r 9ae0d25e4169 -r 45d9b0f6a0d6 miRPlant.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/miRPlant.xml	Thu Jul 31 03:09:14 2014 -0400
@@ -0,0 +1,90 @@
+<tool id="plant_microRNA_v1" name="miRPlant" veision="1.0.0">
+  <description>tool for plant microRNA analisis</description>
+
+  <requirements>
+    <requirement type="set_environment">SCRIPT_PATH</requirement>
+    <requirement type="package" version="0.12.7">bowtie</requirement>
+    <requirement type="package" version="2.11.0">R</requirement>
+	<requirement type="package" version="0.0.13">fastx_toolkit </requirement>
+	<requirement type="package" version="2.1.7">ViennaRNA</requirement>
+  </requirements>
+
+  <!--command interpreter="perl">miPlant.pl -i $input -format $format -gfa $gfa -idx $index -pre $pre -mat $mat -rfam $rfam -idx2 $idx2 -D $D -a $a -M $M -min $min -max $max -mis $mis -e $e -f $f -v $v -r $r -dis $dis -flank $flank -mfe $mfe -t $t -o $output</command-->
+
+  <command interpreter="perl">miRPlant.pl 
+   ## Change this to accommodate the number of threads you have available.
+        -t \${GALAXY_SLOTS:-4}
+   ## Do or not delet rfam mapped tags
+    #if $params.delet_rfam == "yes":
+	-D 
+	#end if
+	-path \$SCRIPT_PATH
+
+    #for $j, $s in enumerate( $series )
+    ##rank_of_series=$j
+    -i ${s.input}
+    -tag ${s.tag}
+    #end for
+
+    -format $format -gfa $gfa -pre $pre -mat $mat -rfam $rfam  -a $a -M $mapnt -min $min -max $max -mis $mismatch -e $e -f $f -v $v -r $r -dis $dis -flank $flank -mfe $mfe
+  </command>
+
+  <inputs>
+
+   <repeat name="series" title="Series">
+     <param name="input" type="data" label="Raw data"/>
+     <param name="tag" type="text" data_ref="input" label="Sample name of raw data"/>
+   </repeat>
+
+	<conditional name="params">
+		<param name="delet_rfam" type="select" label="delet rfam mapped reads">
+		  <option value="yes" selected="true">yes</option>
+		  <option value="no">no</option>
+		 </param>
+    </conditional> <!-- params -->
+
+	<!--param name="input" format="tabular"  type="data" label="input config file" /-->
+	
+	<param name="format" type="select" lable="raw data format" multiple="false">
+	  <option value="fastq">Raw data is fastq. format</option>
+	  <option value="fasta">Raw data is fasta. format</option>
+	</param>
+	
+	<param name="gfa"  type="data" label="genome sequence fasta file"/>
+	<!--param type="data" name="index" label="genome sequence bowtie index"/-->
+	<param name="mat" type="data" label="mature microRNA sequence file" />
+	<param name="pre" type="data" label="precursor microRNA sequence fie" />
+	<param name="rfam" type="data" label="rfam sequence file" />
+	<!--param type="data" name="idx2" label="rfam sequence bowtie index " -->
+	<param name="a" type="text" value="ATCTCGTATG" label="3' adapter sequence" />
+	<param name="mapnt" type="integer" value="8" label="minimum adapter map nts" />
+	<param name="min" type="integer" value="19" label="minimum microRNA length" />
+	<param name="max" type="integer" value="28" label="maximum microRNA length" />
+	<param name="mismatch" type="integer" value="0" label="number of allowed mismatches when mapping reads to precursors" />
+	<param name="e" type="integer" value="2" label="number of nucleotides upstream of the mature sequence to consider" />
+	<param name="f" type="integer" value="5" label="number of nucleotides downstream of the mature sequence to consider" />
+	<param name="v" type="integer" value="0" label="report end-to-end hits less than v mismatches"/>
+	<param name="r" type="integer" value="25" label="a read is allowed to map up to this number of positions in the genome" />
+	<param name="dis" type="integer" value="200" label="Maximal space between miRNA and miRNA*" />
+	<param name="flank" type="integer" value="10" label="Flank sequence length of miRNA precursor" />
+	<param name="mfe" type="float" value="-30" label="Maximal free energy allowed for a miRNA precursor" />
+	<param name="thread" type="integer" value="1" label="number of threads" />
+  </inputs>
+
+  <outputs>
+   <data format="txt" name="known microRNA express list" from_work_dir="miRPlant_out/known_microRNA_express.txt"/>
+   <data format="txt" name="known microRNA express alignment" from_work_dir="miRPlant_out/known_microRNA_express.aln"/>
+   <data format="txt" name="known microRNA moRs result" from_work_dir="miRPlant_out/known_microRNA_express.moRs"/>
+   <data format="txt" name="known microRNA precursor file" from_work_dir="miRPlant_out/known_microRNA_precursor.fa"/>
+   <data format="txt" name="known microRNA mature file" from_work_dir="miRPlant_out/known_microRNA_mature.fa"/>
+   <data format="txt" name="novel microRNA prediction file" from_work_dir="miRPlant_out/known_microRNA_mature.fa"/>
+   <data format="txt" name="novel microRNA express list" from_work_dir="miRPlant_out/novel_microRNA_express.txt"/>
+   <data format="txt" name="novel microRNA precursor file" from_work_dir="miRPlant_out/novel_microRNA_precursor.fa"/>
+   <data format="txt" name="novel microRNA mature sequence file" from_work_dir="miRPlant_out/novel_microRNA_mature.fa"/>
+   <data format="txt" name="analysis result" from_work_dir="miRPlant_out/result.html"/>
+  </outputs>
+
+ <help>
+
+ </help>
+ </tool>