changeset 10:b20345ef3995 draft

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/siRNA.xml	Wed Oct 29 04:18:27 2014 -0400
@@ -0,0 +1,106 @@
+<tool id="plant_sirna_v1" name="siRNA" veision="1.0.0">
+  <description>tool for plant siRNA analisis</description>
+
+  <requirements>
+    <requirement type="set_environment">SCRIPT_PATH</requirement>
+    <requirement type="package" version="0.12.7">bowtie</requirement>
+    <requirement type="package" version="2.11.0">R</requirement>
+	<requirement type="package" version="0.0.13">fastx_toolkit </requirement>
+	<requirement type="package" version="1.96">threads</requirement>
+	<requirement type="package" version="1.06">Parallel-ForkManager</requirement>
+	<requirement type="package" version="2.59">SVG</requirement>
+	<requirement type="package" version="1.4">Boost-Graph</requirement>
+	<requirement type="package" version="1.5.0">libx11</requirement>
+  </requirements>
+
+  <command interpreter="perl">siRNA.pl 
+   ## Change this to accommodate the number of threads you have available.
+        -t \${GALAXY_SLOTS:-4}
+
+	-path \$SCRIPT_PATH
+
+    #for $j, $s in enumerate( $series )
+    ##rank_of_series=$j
+    -i ${s.input}
+    -tag ${s.tag}
+    #end for
+
+   ## Do or not annotate siRNAs by function
+    #if $params.function_anno == "yes":
+     -nat $params.nat -repeat $params.repeat
+	#end if
+   
+   ## Do or not DEG
+    #if $degseq.degseq_analysis == "yes" :
+    -deg $degseq.deg
+    #end if
+
+  -format $format -g $genome -f $gff -mis $mis -rfam $rfam -v $v -a $a -n $mapnt -d $d -p $p -l $l  -cen $cen -span $span > run.log
+
+  </command>
+
+  <inputs>
+
+   <repeat name="series" title="Series">
+     <param name="input" type="data" label="Raw data file"/>
+     <param name="tag" type="text" data_ref="input" label="Sample name of raw data"/>
+   </repeat>
+
+	<param name="format" type="select" lable="raw data format" multiple="false">
+	  <option value="fastq">Raw data is fastq. format</option>
+	  <option value="fasta">Raw data is fasta. format</option>
+	</param>
+	
+	<param name="genome"  type="data" label="genome sequence fasta file"/>
+	<!--param type="data" name="index" label="genome sequence bowtie index"/-->
+	<param name="gff" type="data" label="gff file" />
+	<param name="mis" type="integer" value="0" label="number of allowed mismatches when mapping reads to genome" />
+	<param name="rfam" type="data" label="rfam sequence file" />
+	<param name="v" type="integer" value="0" label="report end-to-end hits less than v mismatches"/>
+	<param name="a" type="text" value="ATCTCGTATG" label="3' adapter sequence" />
+	<param name="mapnt" type="integer" value="25" label="a read is allowed to map up to this number of positions in the genome" />
+	<param name="d" type="integer" value="100" label="distance of tag to merged a cluster" />
+
+	<param name="p" type="select" lable="cluster method" multiple="false">
+	  <option value="F">conventional</option>
+	  <option value="T">NIBLES</option>
+	</param>
+	<param name="l" type="integer" value="1000" label="the length of the upstream and downstream,used in position annotate" />
+
+
+	<conditional name="params">
+		<param name="function_anno" type="select" label="Do or not annotate siRNAs by function">
+		  <option value="no" selected="true">no</option>
+		  <option value="yes">yes</option>
+		 </param>
+		 <when value="yes">
+			<param name="nat" type="data" label="atural antisense transcripts file" />
+			<param name="repeat" type="data" label="repeat information file out of Repeatmasker" />
+		 </when>
+    </conditional> <!-- params -->
+	
+	<param name="cen" type="data" label="centromere file input" />
+	<param name="span" type="integer" value="50000" label="plot span" />
+	
+    <conditional name="degseq">
+       <param name="degseq_analysis" type="select" label="Do or not identify Difference Expression Clusters">
+		  <option value="no" selected="true">no</option>
+		  <option value="yes">yes</option>
+       </param>
+       <when value="yes">
+			<param name="deg" type="data" label="file config of de sample" />
+        </when>
+    </conditional>
+    
+  </inputs>
+
+  <outputs>
+   <data format="txt" name="siRNA cluster" from_work_dir="cluster_runs/total.result" label="${tool.name} on ${on_string}: siRNA cluster"/>
+   <data format="html" name="analysis result" from_work_dir="cluster_runs/result.html" label="${tool.name} on ${on_string}: analysis result"/>
+
+  </outputs>
+
+ <help>
+
+ </help>
+ </tool>