Mercurial > repos > bimib > marea_2_0

--- a/marea_2_0/marea.xml	Thu May 23 16:50:05 2024 +0000
+++ b/marea_2_0/marea.xml	Thu May 23 16:53:57 2024 +0000
@@ -1,316 +1,316 @@
-<tool id="MaREA" name="Metabolic Reaction Enrichment Analysis" version="1.1.7">
-	<macros>
-		<import>marea_macros.xml</import>
-	</macros>
-
-	<requirements>
-		<requirement type="package" version="0.25.3">pandas</requirement>
-		<requirement type="package" version="1.6.3">scipy</requirement>
-		<requirement type="package" version="4.9.3">lxml</requirement>
-		<requirement type="package" version="1.1.0">svglib</requirement>
-		<requirement type="package" version="4.1.0">reportlab</requirement>
-	</requirements>
-
-	<command detect_errors="exit_code">
-		<![CDATA[
-      	python $__tool_directory__/marea.py
-
-      	--tool_dir $__tool_directory__
-      	--option $cond.type_selector
-		--using_RAS $cond.using_RAS.check
-		--using_RPS $cond.using_RPS.check
-        --out_log $log
-
-        #if $cond.type_selector == 'datasets':
-			#if $cond.using_RAS.check == 'true':
-				--input_datas
-				#for $data in $cond.input_Datasets:
-					${data.input}
-				#end for
-
-				--names
-				#for $data in $cond.input_Datasets:
-					${data.input_name}
-				#end for
-			#end if
-
-			#if $cond.using_RPS.check == 'true':
-				--input_datas_rps
-				#for $data in $cond.input_Datasets_RPS:
-					${data.input_rps}
-				#end for
-
-				--names_rps
-				#for $data in $cond.input_Datasets_RPS:
-					${data.input_name_rps}
-				#end for
-			#end if
-
-        #elif $cond.type_selector == 'dataset_class':
-			#if $cond.using_RAS.check == 'true':
-				--input_data ${input_data}
-				--input_class ${input_class}
-			#end if
-
-			#if $cond.using_RPS.check == 'true':
-				--input_data_rps ${input_data_rps}
-				--input_class_rps ${input_class_rps}
-			#end if
-        #end if
-
-		--comparison ${comparis.comparison}
-		#if $comparis.comparison == 'onevsmany'
-			--control '${cond.comparis.controlgroup}'
-		#end if
-
-		--choice_map '${cond_choice_map.choice_map}'
-		#if $cond_choice_map.choice_map == 'Custom':
-			--custom_map ${cond_choice_map.custom_map}
-		#end if
-
-		#if $advanced.choice == 'true':
-			--pValue ${advanced.pValue}
-			--fChange ${advanced.fChange}
-			--generate_svg ${advanced.generateSvg}
-			--generate_pdf ${advanced.generatePdf}
-			--net ${advanced.netRPS}
-		#else
-			--pValue 0.05
-			--fChange 1.2
-			--generate_svg false
-			--generate_pdf true
-			--net false
-		#end if
-        ]]>
-	</command>
-
-	<inputs>
-		<conditional name="cond">
-			<param name="type_selector" argument="--option" type="select" label="Input format:">
-				<option value="datasets" selected="true">RAS of group 1 + RAS of group 2 + ... + RAS of group N</option>
-				<option value="dataset_class">RAS of all samples + sample group specification</option>
-			</param>
-
-			<when value="datasets">
-				<conditional name = "using_RAS">
-					<param name = "check" argument = "--using_RAS" type = "boolean" checked = "true" label = "Using RAS datasets." />
-
-					<when value = "true">
-						<repeat name="input_Datasets" title="RAS dataset" min="2">
-							<param name="input" argument="--input_datas" type="data" format="tabular, csv, tsv" label="add dataset" />
-							<param name="input_name" argument="--names" type="text" label="Dataset's name:" value="Dataset" help="Default: Dataset" />
-						</repeat>
-					</when>
-				</conditional>
-
-				<conditional name = "using_RPS">
-					<param name = "check" argument = "--using_RPS" type = "boolean" checked = "false" label = "Using RPS datasets." />
-
-					<when value = "true">
-						<repeat name="input_Datasets_RPS" title="RPS dataset" min="2">
-							<param name="input_rps" argument="--input_datas_rps" type="data" format="tabular, csv, tsv" label="add dataset" />
-							<param name="input_name_rps" argument="--names_rps" type="text" label="Dataset's name:" value="Dataset" help="Default: Dataset" />
-						</repeat>
-					</when>
-				</conditional>
-			</when>
-
-			<when value="dataset_class">
-				<conditional name = "using_RAS">
-					<param name = "check" argument = "--using_RAS" type = "boolean" checked = "true" label = "Using RAS datasets." />
-
-					<when value = "true">
-						<param name="input_data" argument="--input_data" type="data" format="tabular, csv, tsv" label="RAS of all samples" />
-						<param name="input_class" argument="--input_class" type="data" format="tabular, csv, tsv" label="Sample group specification" />
-					</when>
-				</conditional>
-
-				<conditional name = "using_RPS">
-					<param name = "check" argument = "--using_RPS" type = "boolean" checked = "false" label = "Using RPS datasets." />
-
-					<when value = "true">
-						<param name="input_data_rps" argument="--input_data_rps" type="data" format="tabular, csv, tsv" label="RPS of all samples" />
-						<param name="input_class_rps" argument="--input_class_rps" type="data" format="tabular, csv, tsv" label="Sample group specification" />
-					</when>
-			</conditional>
-			</when>
-		</conditional>
-
-		<conditional name="comparis">
-			<param name="comparison" argument="--comparison" type="select" label="Groups comparison:">
-				<option value="manyvsmany" selected="true">One vs One</option>
-				<option value="onevsrest">One vs All</option>
-				<option value="onevsmany">One vs Control</option>
-			</param>
-			<when value="onevsmany">
-				<param name="controlgroup" argument="--controlgroup" type="text" label="Control group label:" value="0" help="Name of group label to be compared to others"/>
-			</when>
-		</conditional>
-
-		<conditional name="cond_choice_map">
-			<param name="choice_map" argument="--choice_map" type="select" label="Choose metabolic map:">
-				<option value="HMRcore" selected="true">HMRcore</option>
-				<option value="ENGRO2">ENGRO2</option>
-				<option value="Custom">Custom</option>
-			</param>
-
-			<when value="Custom">
-				<param name="custom_map" argument="--custom_map" type="data" format="xml, svg" label="custom-map.svg"/>
-			</when>
-		</conditional>
-
-		<conditional name="advanced">
-			<param name="choice" type="boolean" checked="false" label="Use advanced options?" help="Use this options to choose custom parameters for evaluation: pValue, Fold-Change threshold, how to solve (A and NaN) and specify output maps.">
-				<option value="true" selected="true">No</option>
-				<option value="false">Yes</option>
-			</param>
-
-			<when value="true">
-				<param name="pValue" argument="--pValue" type="float" size="20" value="0.05" max="1" min="0" label="P-value threshold:" help="min value 0" />
-				<param name="fChange" argument="--fChange" type="float" size="20" value="1.2" min="1" label="Fold-Change threshold:" help="min value 1" />
-				<param name="generateSvg" argument="--generateSvg" type="boolean" checked="false" label="Generate SVG map" help="should the program generate an editable svg map of the processes?" />
-				<param name="generatePdf" argument="--generatePdf" type="boolean" checked="true" label="Generate PDF map" help="should the program return a non editable (but displayble) pdf map of the processes?" />
-
-				<param name="netRPS" argument="--net" type="boolean" checked="false" label="Should RPS enrichment use net values?" help="If checked and RPS datasets are present the arrow tips of a reversible arrow will be colored with the net contribution of both directions' RPS values" />
-			</when>
-		</conditional>
-	</inputs>
-
-	<outputs>
-		<data format="txt" name="log" label="MaREA - Log" />
-		<collection name="results" type="list" label="MaREA - Results">
-			<discover_datasets pattern="__name_and_ext__" directory="result"/>
-		</collection>
-	</outputs>
-
-	<help>
-	<![CDATA[
-
-What it does
--------------
-
-This tool analyzes and visualizes differences in the Reaction Activity Scores (RASs) of groups of samples, as computed by the Expression2RAS tool, of groups of samples.
-
-Accepted files are:
-    - option 1) two or more RAS datasets, each referring to samples in a given group. The user can specify a label for each group (as e.g. "classA" and "classB");
-    - option 2) one RAS dataset and one group-file specifying the group each sample belongs to.
-
-RAS datasets format: tab-separated text files, reporting the RAS value of each reaction (row) for a given sample (column).
-
-Column header: sample ID.
-Row header: reaction ID.
-
-Optional files:
-    - custom svg map. Graphical elements must have the same IDs of reactions. See HmrCore svg map for an example.
-
-The tool generates:
-    - 1) a tab-separated file: reporting fold-change and p-values of reaction activity scores (RASs) between a pair of conditions/classes;
-    - 2) a metabolic map file (downloadable as .svg): visualizing up- and down-regulated reactions between a pair of conditions/classes;
-    - 3) a log file (.txt).
-
-Output options:
-To calculate P-Values and Fold-Changes and to enrich maps, comparisons are performed for each possible pair of groups (default option ‘One vs One’).
-
-Alternative options are:
-    - comparison of each group vs. the rest of samples (option ‘One vs Rest’)
-    - comparison of each group vs. a control group (option ‘One vs Control). If this option is selected the user must indicate the control group label.
-
-Output files will be named as classA_vs_classB. Reactions will conventionally be reported as up-regulated (down-regulated) if they are significantly more (less) active in class having label "classA".
-
-Example input
--------------
-
-"RAS of group 1 + RAS of group 2 + ... + RAS of group N" option:
-
-RAS Dataset 1:
-
-+------------+----------------+----------------+----------------+
-| Reaction ID|   TCGAA62670   |   TCGAA62671   |   TCGAA62672   |
-+============+================+================+================+
-| r1642      |    0.523167    |    0.371355    |    0.925661    |
-+------------+----------------+----------------+----------------+
-| r1643      |    0.568765    |    0.765567    |    0.456789    |
-+------------+----------------+----------------+----------------+
-| r1640      |    0.876545    |    0.768933    |    0.987654    |
-+------------+----------------+----------------+----------------+
-| r1641      |    0.456788    |    0.876543    |    0.876542    |
-+------------+----------------+----------------+----------------+
-| r1646      |    0.876543    |    0.786543    |    0.897654    |
-+------------+----------------+----------------+----------------+
-
-RAS Dataset 2:
-
-+------------+----------------+----------------+----------------+
-| Reaction ID|   TCGAA62670   |   TCGAA62671   |   TCGAA62672   |
-+============+================+================+================+
-| r1642      |    0.523167    |    0.371355    |    0.925661    |
-+------------+----------------+----------------+----------------+
-| r1643      |    0.568765    |    0.765567    |    0.456789    |
-+------------+----------------+----------------+----------------+
-| r1640      |    0.876545    |    0.768933    |    0.987654    |
-+------------+----------------+----------------+----------------+
-| r1641      |    0.456788    |    0.876543    |    0.876542    |
-+------------+----------------+----------------+----------------+
-| r1646      |    0.876543    |    0.786543    |    0.897654    |
-+------------+----------------+----------------+----------------+
-
-"RAS of all samples + sample group specification" option:
-
-RAS Dataset:
-
-+------------+----------------+----------------+----------------+
-| Reaction ID|   TCGAA62670   |   TCGAA62671   |   TCGAA62672   |
-+============+================+================+================+
-| r1642      |    0.523167    |    0.371355    |    0.925661    |
-+------------+----------------+----------------+----------------+
-| r1643      |    0.568765    |    0.765567    |    0.456789    |
-+------------+----------------+----------------+----------------+
-| r1640      |    0.876545    |    0.768933    |    0.987654    |
-+------------+----------------+----------------+----------------+
-| r1641      |    0.456788    |    0.876543    |    0.876542    |
-+------------+----------------+----------------+----------------+
-| r1646      |    0.876543    |    0.786543    |    0.897654    |
-+------------+----------------+----------------+----------------+
-
-Group-file
-
-+---------------+-----------+
-| Patient ID    |   Class   |
-+===============+===========+
-| TCGAAA3529    |    MSI    |
-+---------------+-----------+
-| TCGAA62671    |    MSS    |
-+---------------+-----------+
-| TCGAA62672    |    MSI    |
-+---------------+-----------+
-
-Advanced options
-----------------
-
-P-Value threshold: the threshold used for significance Kolmogorov-Smirnov (KS) test, to verify whether the distributions of RASs over the samples in two sets are significantly different
-
-Fold-Change threshold: threshold of the fold-change between the average RAS of two groups. Among the reactions that pass the KS test, only fold-change values larger than the indicated threshold will be visualized on the output metabolic map;
-
-
-.. class:: infomark
-
-**TIP**: If your data is not TAB delimited, use `Convert delimiters to TAB`_.
-
-.. class:: infomark
-
-**TIP**: If your dataset is not split into classes, use `MaREA cluster analysis`_.
-
-.. class:: infomark
-
-**TIP**: This tool using the RAS scores computed by `Ras tool`_.
-
-@REFERENCE@
-
-.. _Ras tool: http://bimib.disco.unimib.it:5555/?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbimib%2Fmarea%2FMaREA+RAS+Generator%2F1.0.6&version=1.0.6&__identifer=auulv6gbp76
-.. _Convert delimiters to TAB: http://bimib.disco.unimib.it:5555/?tool_id=Convert+characters1&version=1.0.0&__identifer=76g7trea4j6
-.. _MaREA cluster analysis: http://bimib.disco.unimib.it:5555/?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbimib%2Fmarea%2FMaREA_cluester%2F1.1.2&version=1.1.2&__identifer=lxbyzn2me9
-
-]]>
-	</help>
-	<expand macro="citations" />
+<tool id="MaREA" name="Metabolic Reaction Enrichment Analysis" version="1.1.7">
+	<macros>
+		<import>marea_macros.xml</import>
+	</macros>
+
+	<requirements>
+		<requirement type="package" version="0.25.3">pandas</requirement>
+		<requirement type="package" version="1.6.3">scipy</requirement>
+		<requirement type="package" version="4.9.3">lxml</requirement>
+		<requirement type="package" version="1.1.0">svglib</requirement>
+		<requirement type="package" version="4.1.0">reportlab</requirement>
+	</requirements>
+
+	<command detect_errors="exit_code">
+		<![CDATA[
+      	python $__tool_directory__/marea.py
+
+      	--tool_dir $__tool_directory__
+      	--option $cond.type_selector
+		--using_RAS $cond.using_RAS.check
+		--using_RPS $cond.using_RPS.check
+        --out_log $log
+
+        #if $cond.type_selector == 'datasets':
+			#if $cond.using_RAS.check == 'true':
+				--input_datas
+				#for $data in $using_RAS.input_Datasets:
+					${data.input}
+				#end for
+
+				--names
+				#for $data in $using_RAS.input_Datasets:
+					${data.input_name}
+				#end for
+			#end if
+
+			#if $cond.using_RPS.check == 'true':
+				--input_datas_rps
+				#for $data in $using_RPS.input_Datasets_RPS:
+					${data.input_rps}
+				#end for
+
+				--names_rps
+				#for $data in $using_RPS.input_Datasets_RPS:
+					${data.input_name_rps}
+				#end for
+			#end if
+
+        #elif $cond.type_selector == 'dataset_class':
+			#if $cond.using_RAS.check == 'true':
+				--input_data ${using_RAS.input_data}
+				--input_class ${using_RAS.input_class}
+			#end if
+
+			#if $cond.using_RPS.check == 'true':
+				--input_data_rps ${using_RPS.input_data_rps}
+				--input_class_rps ${using_RPS.input_class_rps}
+			#end if
+        #end if
+
+		--comparison ${comparis.comparison}
+		#if $comparis.comparison == 'onevsmany'
+			--control '${cond.comparis.controlgroup}'
+		#end if
+
+		--choice_map '${cond_choice_map.choice_map}'
+		#if $cond_choice_map.choice_map == 'Custom':
+			--custom_map ${cond_choice_map.custom_map}
+		#end if
+
+		#if $advanced.choice == 'true':
+			--pValue ${advanced.pValue}
+			--fChange ${advanced.fChange}
+			--generate_svg ${advanced.generateSvg}
+			--generate_pdf ${advanced.generatePdf}
+			--net ${advanced.netRPS}
+		#else
+			--pValue 0.05
+			--fChange 1.2
+			--generate_svg false
+			--generate_pdf true
+			--net false
+		#end if
+        ]]>
+	</command>
+
+	<inputs>
+		<conditional name="cond">
+			<param name="type_selector" argument="--option" type="select" label="Input format:">
+				<option value="datasets" selected="true">RAS of group 1 + RAS of group 2 + ... + RAS of group N</option>
+				<option value="dataset_class">RAS of all samples + sample group specification</option>
+			</param>
+
+			<when value="datasets">
+				<conditional name = "using_RAS">
+					<param name = "check" argument = "--using_RAS" type = "boolean" checked = "true" label = "Using RAS datasets." />
+
+					<when value = "true">
+						<repeat name="input_Datasets" title="RAS dataset" min="2">
+							<param name="input" argument="--input_datas" type="data" format="tabular, csv, tsv" label="add dataset" />
+							<param name="input_name" argument="--names" type="text" label="Dataset's name:" value="Dataset" help="Default: Dataset" />
+						</repeat>
+					</when>
+				</conditional>
+
+				<conditional name = "using_RPS">
+					<param name = "check" argument = "--using_RPS" type = "boolean" checked = "false" label = "Using RPS datasets." />
+
+					<when value = "true">
+						<repeat name="input_Datasets_RPS" title="RPS dataset" min="2">
+							<param name="input_rps" argument="--input_datas_rps" type="data" format="tabular, csv, tsv" label="add dataset" />
+							<param name="input_name_rps" argument="--names_rps" type="text" label="Dataset's name:" value="Dataset" help="Default: Dataset" />
+						</repeat>
+					</when>
+				</conditional>
+			</when>
+
+			<when value="dataset_class">
+				<conditional name = "using_RAS">
+					<param name = "check" argument = "--using_RAS" type = "boolean" checked = "true" label = "Using RAS datasets." />
+
+					<when value = "true">
+						<param name="input_data" argument="--input_data" type="data" format="tabular, csv, tsv" label="RAS of all samples" />
+						<param name="input_class" argument="--input_class" type="data" format="tabular, csv, tsv" label="Sample group specification" />
+					</when>
+				</conditional>
+
+				<conditional name = "using_RPS">
+					<param name = "check" argument = "--using_RPS" type = "boolean" checked = "false" label = "Using RPS datasets." />
+
+					<when value = "true">
+						<param name="input_data_rps" argument="--input_data_rps" type="data" format="tabular, csv, tsv" label="RPS of all samples" />
+						<param name="input_class_rps" argument="--input_class_rps" type="data" format="tabular, csv, tsv" label="Sample group specification" />
+					</when>
+			</conditional>
+			</when>
+		</conditional>
+
+		<conditional name="comparis">
+			<param name="comparison" argument="--comparison" type="select" label="Groups comparison:">
+				<option value="manyvsmany" selected="true">One vs One</option>
+				<option value="onevsrest">One vs All</option>
+				<option value="onevsmany">One vs Control</option>
+			</param>
+			<when value="onevsmany">
+				<param name="controlgroup" argument="--controlgroup" type="text" label="Control group label:" value="0" help="Name of group label to be compared to others"/>
+			</when>
+		</conditional>
+
+		<conditional name="cond_choice_map">
+			<param name="choice_map" argument="--choice_map" type="select" label="Choose metabolic map:">
+				<option value="HMRcore" selected="true">HMRcore</option>
+				<option value="ENGRO2">ENGRO2</option>
+				<option value="Custom">Custom</option>
+			</param>
+
+			<when value="Custom">
+				<param name="custom_map" argument="--custom_map" type="data" format="xml, svg" label="custom-map.svg"/>
+			</when>
+		</conditional>
+
+		<conditional name="advanced">
+			<param name="choice" type="boolean" checked="false" label="Use advanced options?" help="Use this options to choose custom parameters for evaluation: pValue, Fold-Change threshold, how to solve (A and NaN) and specify output maps.">
+				<option value="true" selected="true">No</option>
+				<option value="false">Yes</option>
+			</param>
+
+			<when value="true">
+				<param name="pValue" argument="--pValue" type="float" size="20" value="0.05" max="1" min="0" label="P-value threshold:" help="min value 0" />
+				<param name="fChange" argument="--fChange" type="float" size="20" value="1.2" min="1" label="Fold-Change threshold:" help="min value 1" />
+				<param name="generateSvg" argument="--generateSvg" type="boolean" checked="false" label="Generate SVG map" help="should the program generate an editable svg map of the processes?" />
+				<param name="generatePdf" argument="--generatePdf" type="boolean" checked="true" label="Generate PDF map" help="should the program return a non editable (but displayble) pdf map of the processes?" />
+
+				<param name="netRPS" argument="--net" type="boolean" checked="false" label="Should RPS enrichment use net values?" help="If checked and RPS datasets are present the arrow tips of a reversible arrow will be colored with the net contribution of both directions' RPS values" />
+			</when>
+		</conditional>
+	</inputs>
+
+	<outputs>
+		<data format="txt" name="log" label="MaREA - Log" />
+		<collection name="results" type="list" label="MaREA - Results">
+			<discover_datasets pattern="__name_and_ext__" directory="result"/>
+		</collection>
+	</outputs>
+
+	<help>
+	<![CDATA[
+
+What it does
+-------------
+
+This tool analyzes and visualizes differences in the Reaction Activity Scores (RASs) of groups of samples, as computed by the Expression2RAS tool, of groups of samples.
+
+Accepted files are:
+    - option 1) two or more RAS datasets, each referring to samples in a given group. The user can specify a label for each group (as e.g. "classA" and "classB");
+    - option 2) one RAS dataset and one group-file specifying the group each sample belongs to.
+
+RAS datasets format: tab-separated text files, reporting the RAS value of each reaction (row) for a given sample (column).
+
+Column header: sample ID.
+Row header: reaction ID.
+
+Optional files:
+    - custom svg map. Graphical elements must have the same IDs of reactions. See HmrCore svg map for an example.
+
+The tool generates:
+    - 1) a tab-separated file: reporting fold-change and p-values of reaction activity scores (RASs) between a pair of conditions/classes;
+    - 2) a metabolic map file (downloadable as .svg): visualizing up- and down-regulated reactions between a pair of conditions/classes;
+    - 3) a log file (.txt).
+
+Output options:
+To calculate P-Values and Fold-Changes and to enrich maps, comparisons are performed for each possible pair of groups (default option ‘One vs One’).
+
+Alternative options are:
+    - comparison of each group vs. the rest of samples (option ‘One vs Rest’)
+    - comparison of each group vs. a control group (option ‘One vs Control). If this option is selected the user must indicate the control group label.
+
+Output files will be named as classA_vs_classB. Reactions will conventionally be reported as up-regulated (down-regulated) if they are significantly more (less) active in class having label "classA".
+
+Example input
+-------------
+
+"RAS of group 1 + RAS of group 2 + ... + RAS of group N" option:
+
+RAS Dataset 1:
+
++------------+----------------+----------------+----------------+
+| Reaction ID|   TCGAA62670   |   TCGAA62671   |   TCGAA62672   |
++============+================+================+================+
+| r1642      |    0.523167    |    0.371355    |    0.925661    |
++------------+----------------+----------------+----------------+
+| r1643      |    0.568765    |    0.765567    |    0.456789    |
++------------+----------------+----------------+----------------+
+| r1640      |    0.876545    |    0.768933    |    0.987654    |
++------------+----------------+----------------+----------------+
+| r1641      |    0.456788    |    0.876543    |    0.876542    |
++------------+----------------+----------------+----------------+
+| r1646      |    0.876543    |    0.786543    |    0.897654    |
++------------+----------------+----------------+----------------+
+
+RAS Dataset 2:
+
++------------+----------------+----------------+----------------+
+| Reaction ID|   TCGAA62670   |   TCGAA62671   |   TCGAA62672   |
++============+================+================+================+
+| r1642      |    0.523167    |    0.371355    |    0.925661    |
++------------+----------------+----------------+----------------+
+| r1643      |    0.568765    |    0.765567    |    0.456789    |
++------------+----------------+----------------+----------------+
+| r1640      |    0.876545    |    0.768933    |    0.987654    |
++------------+----------------+----------------+----------------+
+| r1641      |    0.456788    |    0.876543    |    0.876542    |
++------------+----------------+----------------+----------------+
+| r1646      |    0.876543    |    0.786543    |    0.897654    |
++------------+----------------+----------------+----------------+
+
+"RAS of all samples + sample group specification" option:
+
+RAS Dataset:
+
++------------+----------------+----------------+----------------+
+| Reaction ID|   TCGAA62670   |   TCGAA62671   |   TCGAA62672   |
++============+================+================+================+
+| r1642      |    0.523167    |    0.371355    |    0.925661    |
++------------+----------------+----------------+----------------+
+| r1643      |    0.568765    |    0.765567    |    0.456789    |
++------------+----------------+----------------+----------------+
+| r1640      |    0.876545    |    0.768933    |    0.987654    |
++------------+----------------+----------------+----------------+
+| r1641      |    0.456788    |    0.876543    |    0.876542    |
++------------+----------------+----------------+----------------+
+| r1646      |    0.876543    |    0.786543    |    0.897654    |
++------------+----------------+----------------+----------------+
+
+Group-file
+
++---------------+-----------+
+| Patient ID    |   Class   |
++===============+===========+
+| TCGAAA3529    |    MSI    |
++---------------+-----------+
+| TCGAA62671    |    MSS    |
++---------------+-----------+
+| TCGAA62672    |    MSI    |
++---------------+-----------+
+
+Advanced options
+----------------
+
+P-Value threshold: the threshold used for significance Kolmogorov-Smirnov (KS) test, to verify whether the distributions of RASs over the samples in two sets are significantly different
+
+Fold-Change threshold: threshold of the fold-change between the average RAS of two groups. Among the reactions that pass the KS test, only fold-change values larger than the indicated threshold will be visualized on the output metabolic map;
+
+
+.. class:: infomark
+
+**TIP**: If your data is not TAB delimited, use `Convert delimiters to TAB`_.
+
+.. class:: infomark
+
+**TIP**: If your dataset is not split into classes, use `MaREA cluster analysis`_.
+
+.. class:: infomark
+
+**TIP**: This tool using the RAS scores computed by `Ras tool`_.
+
+@REFERENCE@
+
+.. _Ras tool: http://bimib.disco.unimib.it:5555/?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbimib%2Fmarea%2FMaREA+RAS+Generator%2F1.0.6&version=1.0.6&__identifer=auulv6gbp76
+.. _Convert delimiters to TAB: http://bimib.disco.unimib.it:5555/?tool_id=Convert+characters1&version=1.0.0&__identifer=76g7trea4j6
+.. _MaREA cluster analysis: http://bimib.disco.unimib.it:5555/?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbimib%2Fmarea%2FMaREA_cluester%2F1.1.2&version=1.1.2&__identifer=lxbyzn2me9
+
+]]>
+	</help>
+	<expand macro="citations" />
 </tool>
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