view SpliceTrap.pl @ 4:cd336e593a92 draft

planemo upload

#!/usr/bin/perl
# Author: [e-mail]
# Modified: Baekdoo Kim ([e-mail])
use strict;
use Getopt::Long;
use Data::Dumper;
####################
use Cwd;
my $PROG = $0;
my $CUR_DIR = Cwd::abs_path(Cwd::cwd());
my $PROG_ABS_PATH = Cwd::abs_path($PROG);
#my $SrcFolder=`dirname $PROG_ABS_PATH`;
#chomp($SrcFolder);
#my %config=do "$ENV{HOME}/.SpliceTrap.pl.ini";
#my $SrcFolder=$config{SrcFolder};

my @programs = ('R','echo','cat','bash','perl','ln','mkdir','paste','grep','sort','basename','awk','wc','mv','cd','rm','split','head' );
foreach my $program (@programs)
{
        die ("CHECK: $program not found\n") if(system("hash $program >/dev/null"));

}

####################
my $SrcFolder="";
my $MapSoftware="bowtie";
my $DatabasePrefix="hg38";
my $ReadFileFormat="";
my $ReadFile1Name="";
my $ReadFile2Name="";
my $CutoffLevel="M";
my $Outputfolder=$CUR_DIR;
my $OutputPrefix="Result";
#my $CutoffOnly=0;
my $ReadSize=36;
my $JunctionCut=5;
my $onGalaxy_raw="";
my $onGalaxy_txt="";
my $BowtieThreads=1;
my $noIRMstr="";
my $noIRM = 0;

my $num_args = $#ARGV;
$onGalaxy_raw = $ARGV[$num_args-1];
$onGalaxy_txt = $ARGV[$num_args];

GetOptions (
	"l:s"=>\$SrcFolder,
        "m:s"=>\$MapSoftware,
        "d:s"=>\$DatabasePrefix,
#       "f:s"=>\$ReadFileFormat,
        "1:s"=>\$ReadFile1Name,
        "2:s"=>\$ReadFile2Name,
        "c:s"=>\$CutoffLevel,
        "outdir:s"=>\$Outputfolder,
        "o:s"=>\$OutputPrefix,
        "j:i"=>\$JunctionCut,
        "s:i"=>\$ReadSize,
        "p:i"=>\$BowtieThreads,
        "noIRM|noirm"=>\$noIRM
#       "local:s"=>\$local,
#       "rerun"=>\$CutoffOnly
);
#-O for galaxy output


my $InputParaDes="      Usage of the script:
	-l	Base Location (required)
        -m      Mapping software: [bowtie]/rmap
        -d      Database prefix: [hg18]/mm9/rn4/userdefined
        -1      Read File 1
        -2      Read File 2
        -c      Cutoff Level:H/[M]/L
                Means High, Middle or Low
        -j      Junction reads requirement per junction for each exon-isoform [5]
        -o      Output prefix {Result}
        -s      Read Size [36]
        --outdir Output folder [./]
        -p      Bowtie parameter, threads number, only use this when you don't use qsub [1]
        --noIRM Skip the IRM correction step
        
        This is a quick help, please refer to the README file for details.
";


if($SrcFolder eq "") {
	print "[CHECK] - Please provide the location of the script (option '-l')\n\n";
	exit;
}

if($ReadFile2Name eq "")
{
        $ReadFile2Name = $ReadFile1Name;
        #trigger singled end mode
}

if($ReadFile1Name eq "" or $ReadFile2Name eq "" )
{
        print $InputParaDes;
        exit;
}

if($BowtieThreads < 1)
{
        print $InputParaDes;
        exit;
}

if (! -e "$SrcFolder/db/$DatabasePrefix/parallel")
{
        print "CHECK: Error, the database you specified is not properly installed.\n";
        #print $InputParaDes;
        exit;

}

if($CutoffLevel ne "H" and  $CutoffLevel ne "M" and  $CutoffLevel ne "L")
{
        print $InputParaDes;
        exit;
}


$ReadFile1Name = Cwd::abs_path($ReadFile1Name);
$ReadFile2Name = Cwd::abs_path($ReadFile2Name);

#check the files
open(check,$ReadFile1Name) or die ("CHECK: Error when opening $ReadFile1Name\n");
my $checkoneline = <check>;
if(substr($checkoneline,0,1) eq ">")
{
        $ReadFileFormat = "fasta";
}
elsif(substr($checkoneline,0,1) eq "@")
{
        $ReadFileFormat = "fastq";
}
else
{
        die("CHECK: ERROR:Please check $ReadFile1Name\n");
}
close(check);

open(check,$ReadFile2Name) or die ("CHECK: Error when opening $ReadFile2Name\n");
my $checkoneline = <check>;
if(substr($checkoneline,0,1) eq ">")
{
        die("CHECK: $ReadFile2Name has a different format as $ReadFile1Name\n") if ($ReadFileFormat ne "fasta");
}
elsif(substr($checkoneline,0,1) eq "@")
{
        die("CHECK: $ReadFile2Name has a different format as $ReadFile1Name\n") if ($ReadFileFormat ne "fastq");
}
else
{
        die("CHECK: ERROR:Please check $ReadFile2Name\n");
}
close(check);

$Outputfolder= Cwd::abs_path($Outputfolder);
if($Outputfolder eq "/tmp")
{
        while(-e $Outputfolder)
        {
                my $random_foldername = random_sessid();
                $Outputfolder = "/tmp/".$random_foldername;
        }
}


if(! -e $Outputfolder)
{
        mkdir $Outputfolder or die "CHECK: cannot mkdir $Outputfolder\n";
}
if(! -d $Outputfolder)
{
        die "CHECK: $Outputfolder is not a folder\n";
}

if($MapSoftware eq "bowtie")
{
        print "CHECK: whether bowtie installed and in PATH\n";
        my $bowtiechecker=`bowtie --version`;
        if($bowtiechecker ne "")
        {
                print "CHECK: bowtie found, information below:\n";
                print $bowtiechecker,"\n";
        }
        else
        {
                die "CHECK: No bowtie found in PATH, EXIT!\n";
        }
}
elsif($MapSoftware eq "rmap")
{
        print "CHECK: checking rmap...\n";
        if(system("type rmap &>/dev/null") ==0 )
        {
                print "CHECK: rmap found, continue\n";
        }
        else
        {
                die "CHECK: No rmap found in PATH, EXIT!\n";
        }
}
else
{
        die "CHECK: option -m only takes rmap or bowtie as inputs\n";
}

if($ReadSize == 0)
{
        die "CHECK: Please check option -s Read size\n";
}

if($noIRM)
{
        $noIRMstr= "noirm";
}

#write more checks later
print "PARAMETERS:\tMapping software:  ",$MapSoftware,"\n";
print "PARAMETERS:\tDatabase prefix:   ",$DatabasePrefix,"\n";
print "PARAMETERS:\tRead end 1:        ",$ReadFile1Name,"\n";
print "PARAMETERS:\tRead end 2:        ",$ReadFile2Name,"\n" if($ReadFile2Name ne $ReadFile1Name);
print "PARAMETERS:\tGalaxy_raw:        ",$onGalaxy_raw,"\n"; #if($onGalaxy_raw ne "");
print "PARAMETERS:\tGalaxy_txt:        ",$onGalaxy_txt,"\n"; #if($onGalaxy_txt ne "");
print "PARAMETERS:\tCutoff level:      ",$CutoffLevel,"\n";
print "PARAMETERS:\tJunction reads.min:",$JunctionCut,"\n";
print "PARAMETERS:\tOutput folder:     ",$Outputfolder,"\n";
print "PARAMETERS:\tOutput prefix:     ",$OutputPrefix,"\n";
print "PARAMETERS:\tRead size:         ",$ReadSize,"\n";
print "PARAMETERS:\tBowtie threads #:  ",$BowtieThreads,"\n";
print "PARAMETERS:\tNo IRM.\n" if ($noIRM);

if($MapSoftware eq "bowtie")
{
        print "=================STAGE 1 MAPPING===================\n";
        system("bash $SrcFolder/bin/mapping_bowtie.sh $ReadFile1Name $ReadFileFormat $DatabasePrefix $Outputfolder $SrcFolder $BowtieThreads");
        system("bash $SrcFolder/bin/mapping_bowtie.sh $ReadFile2Name $ReadFileFormat $DatabasePrefix $Outputfolder $SrcFolder $BowtieThreads") if($ReadFile2Name ne $ReadFile1Name);
        print "=================STAGE 2 ESTIMATION================\n";
        #  ratio, log, nums
        system("bash $SrcFolder/bin/batch_para_cov10p_fit.sh $ReadFile1Name $ReadFile2Name $OutputPrefix $ReadSize $DatabasePrefix $Outputfolder $SrcFolder $noIRMstr") ;
        print "=================STAGE 3 CUTOFF====================\n";
        #   raw
        system("bash $SrcFolder/bin/apply_cutoff.sh $OutputPrefix $CutoffLevel $Outputfolder $JunctionCut $SrcFolder $noIRMstr");


}

if($MapSoftware eq "rmap")
{
        print "=================STAGE 1 MAPPING===================\n";

        system("bash $SrcFolder/bin/mapping_rmap.sh $ReadFile1Name $ReadFileFormat $DatabasePrefix $Outputfolder $SrcFolder") ;
        system("bash $SrcFolder/bin/mapping_rmap.sh $ReadFile2Name $ReadFileFormat $DatabasePrefix $Outputfolder $SrcFolder") if($ReadFile2Name ne $ReadFile1Name);
        print "=================STAGE 2 ESTIMATION================\n";

        system("bash $SrcFolder/bin/batch_para_cov10p_fit.sh $ReadFile1Name $ReadFile2Name $OutputPrefix $ReadSize $DatabasePrefix $Outputfolder $SrcFolder $noIRMstr") ;
        print "=================STAGE 3 CUTOFF====================\n";
        system("bash $SrcFolder/bin/apply_cutoff.sh $OutputPrefix $CutoffLevel $Outputfolder $JunctionCut $SrcFolder $noIRMstr");


}

#print "============ALL DONE, OUTPUTFILE:$OutputPrefix.txt\n";

if($onGalaxy_raw ne "" && $onGalaxy_txt ne "")
{
        print "OUTPUTFILE:$OutputPrefix.raw\n";
        system("grep -v na $Outputfolder/$OutputPrefix.raw >$onGalaxy_raw");
        print "OUTPUTFILE:$OutputPrefix.txt\n";
        system("grep -v na $Outputfolder/$OutputPrefix.txt >$onGalaxy_txt");
}

print "============Clean up\n";
system("rm -r $Outputfolder/$OutputPrefix.*");

sub random_sessid {
        #my @chars = (0..9,a..z,A..Z);
        my @chars = ('a'..'z','A'..'Z');
        my $len = 10;
        my $string = join '', map {$chars[rand(@chars)]} (1..$len);
        return $string;
}