comparison hetbox.xml @ 0:8d2c4c11fd8f draft default tip

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0:8d2c4c11fd8f

<tool id="hetbox" version="1.0" name="MAF boxplot">
  <description>Minor Allele Frequency Boxplot</description>
  <requirements>
    <requirement type="package" version="2.15.0">R</requirement>
    <requirement type="package" version="2.2.6">rpy2</requirement>
    <requirement type="package" version="1.7.1">numpy</requirement>
  </requirements>
  <command interpreter="python">hetbox.py $input $outplot $outreport
  </command>
  <inputs>
    <param name="input" type="data" format="tabular" label="Input allele counts table"/>
    <param name="report" type="boolean" truevalue="yes" falsevalue="no" checked="True" label="Also generate a report on data spread" />
  </inputs>
  <outputs>
    <data name="outplot" format="png" label="${tool.name} on ${on_string}: boxplot"/>
    <data name="outreport" format="tabular" label="${tool.name} on ${on_string}: report">
      <filter>report is True</filter>
    </data>
  </outputs>
  <stdio>
    <exit_code range="1:" err_level="fatal"/>
    <exit_code range=":-1" err_level="fatal"/>
  </stdio>

  <help>

.. class:: infomark

**What it does**

The MAF Boxplot tool takes a table listing heteroplasmic sites per sample and their corresponding minor allele frequency (MAF).
It generates a boxplot of the minor allele frequencies per sample by default. The number of heteroplasmic sites is displayed under each box.
Optionally, it can generate  a report that includes the total number of heteroplasmic sites, the median and the median absolute deviation (MAD) of the minor allele frequencies per sample.

-----

.. class:: warningmark

**Note**

Please, follow the format described below for the input file:

-----

.. class:: infomark

**Formats**

**Variant Annotator tool output format**

Columns::

    1.  sample id
    2.  chromosome
    3.  position
    4   counts for A's
    5.  counts for C's
    6.  counts for G's
    7.  counts for T's
    8.  Coverage
    9.  Number of alleles passing frequency threshold
    10. Major allele
    11. Minor allele
    12. Minor allele frequency in position

-----

**Citation**

If you use this tool, please cite Dickins B, Rebolledo-Jaramillo B, et al. *In preparation.*
(boris-at-bx.psu.edu)


  </help>
</tool>