# HG changeset patch # User bornea # Date 1447776236 18000 # Node ID a1bcd44fc605161a81ee0f6e6983466ede2894c7 # Parent 13383ba55336008a424a4abe38cda21d4ec2259e Deleted selected files diff -r 13383ba55336 -r a1bcd44fc605 SAINT_preprocessing_v5.xml --- a/SAINT_preprocessing_v5.xml Tue Nov 17 10:32:56 2015 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,91 +0,0 @@ - - - - #if (str($type) == 'Scaffold'): - SAINT_preprocessing_v6.py $input $preybool $fasta_db $Inter_file $Prey_file - " - #for $ba in $bait - ${ba.bait1} - ${ba.assign} - ${ba.T_C} - #end for - " - $Bait_file \$INSTALL_RUN_PATH/ - #elif (str($type) == 'MaxQuant'): - SAINT_preprocessing_v6_mq_pep.py $input $preybool $fasta_db $Inter_file $Prey_file - " - #for $ba in $bait - ${ba.bait1} - ${ba.assign} - ${ba.T_C} - #end for - " - $Bait_file \$INSTALL_RUN_PATH/ - #end if - - - INSTALL_RUN_PATH - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -Pre-processing: -APOSTL is able to recognize either a Scaffold "Samples Report" file (tab-delimited -txt file) or the "peptides.txt" file output in the maxquant "txt" output folder. No -modifications should be made to these files. Using the "Bait Create" tool, you can -create your "bait.txt" file. It is important that the individual bait names match the -bait names within your scaffold or maxquant output. APOSTL uses the bait file to find -the user's baits of interest. Additionally there is an option to make the prey file (Y/N). -When making a prey file, APOSTL queries uniprot in order to extract protein amino acid -lengths and gene names. This takes several minutes depending on your internet connection. -Some users may want to run SAINTexpress using the same data set while changing which baits -are considered test or control. It is useful to toggle "Make Prey" off in order to save -time by circumventing this step as the same prey file can be used for both SAINTexpress -runs. - -INPUTS: - -Scaffold file: - -- Scaffold "Samples Report" output (tab-delimited txt file) - - -Maxquant file: - -- maxquant "peptides.txt" file (tab-delimited txt file) - -