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1 <tool id="bedtools_getfasta" name="extract fasta from bed" version="0.3.0">
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2
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3 <description>
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4 </description>
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5
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6 <requirements>
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7 <requirement type="package" version="2.22">bedtools</requirement>
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8 </requirements>
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9
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10 <command>
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11 bedtools getfasta -fi '$input' -bed '$bedfile' -fo output.out
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12 </command>
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13
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14 <inputs>
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15 <param format="fasta" name="input" type="data" label="fasta file from where to extract region"/>
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16 <param name="bedfile" format="bed" type="data" label="bed file with regions"/>
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17 </inputs>
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18
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19 <outputs>
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20 <data format="fasta" name="output" from_work_dir="output.out" label="${input.name} (as fasta)"/>
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21 </outputs>
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22
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23 <help>
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24
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25 **What it does**
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26
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27 This tool converts a BED file into extracted fasta regions.
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28
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29
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30 .. class:: warningmark
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31
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32 This tool requires that `bedtools`__ has been installed on your system.
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33
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34 ------
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35
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36 This tool is part of the `bedtools package`__ from the `Quinlan laboratory`__. If you use this tool, please cite `Quinlan AR, and Hall I.M. BEDTools: A flexible framework for comparing genomic features. Bioinformatics, 2010, 26, 6.`__
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37
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38 .. __: http://code.google.com/p/bedtools/
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39 .. __: http://code.google.com/p/bedtools/
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40 .. __: http://cphg.virginia.edu/quinlan/
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41 .. __: http://bioinformatics.oxfordjournals.org/content/26/6/841.short
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42
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43
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44
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45 </help>
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46 </tool> |