annotate validateFASTA.xml @ 2:d61a95fe20e4 draft

planemo upload commit 833e8a1d5ef37cbd4cadad6c90a51b268871627b-dirty

<tool id="validate_fasta_database" name="Validate FASTA Headers" version="0.1.1">
    <requirements>
    </requirements>
    <stdio>
        <exit_code range="1" level="fatal" description="Invalid FASTA headers detected, was asked to fail"/>
    </stdio>
    <command detect_errors="exit_code"><![CDATA[
        java -jar $__tool_directory__/FastaHeader-1.0-SNAPSHOT-jar-with-dependencies.jar "$FASTA" "$goodFasta" "$badFasta" "$crashIfInvalid"  
    ]]></command>
    <inputs>
        <param type="data" name="FASTA" format="fasta" label="Select input FASTA dataset"/>
        <param type="boolean" name="crashIfInvalid" label="Fail job if invalid FASTA headers detected?"/>        
    </inputs>
    <outputs>
        <data name="goodFasta" format="fasta" label="Validate FASTA: Passed Sequences"/>
        <data name="badFasta" format="fasta" label="Validate FASTA: Failed Sequences"/>
    </outputs>
    <tests>
        <test>
            <param name="FASTA" value="fastaFilteringTest_IN.fasta"/>
            <output name="goodFasta" file="fastaFilteringTest_OUT1.fasta" />
            <output name="badFasta" file="fastaFilteringTest_OUT2.fasta" />
        </test>
    </tests>
    <help>
<![CDATA[
**Notes**

Takes a FASTA database and validates the headers using the Compomics (developers of SearchGUI and PeptideShaker) schema. 
Custom FASTA databases may be in an invalid format, which causes SearchGUI to crash.
        
**Output**

The main output of this tool, "Validate FASTA: Passed Sequences", is a FASTA database that can be run through SearchGUI without error.
The failed sequences may be examined for typos and other errors. 

In addition, the tool will print the databases assigned by the Compomics utility (i.e., UniProt), for a quick check of the validity of the custom FASTA database. 

Sequences that may cause the tool to report an exception are those that are not valid examples of the following formats: 
    * UniProt,
    * SwissProt (starts with ">sw|" or ">SW|")
    * NCBI (starts with ">gi|" or ">GI|")
    * Halobacterium from Max Planck (starts with "OE")
    * H Influenza, from Novartis (starts with ">hflu_")
    * C Trachomatis (starts with ">C.tr\_" or "C\_trachomatis\_")
    * M Tuberculosis (starts with ">M. tub")
    * Saccharomyces Genome Database (contains "SGDID")
    * Genome translation (ex. ">dm345\_3L-sense [2343534-234353938]")
    * Genome Annotation Framework for Flexible Analysis (GAFFA) (starts with ">GAFFA")
    * UPS (contains "\_HUMAN\_UPS")
           
Many sequences are reported as Generic, which may or may not allow for extraction of the accession number. 
]]>
    </help>
    <citations>
        <citation type="bibtex">
            @misc{fastaValidation,
            author = {The GalaxyP Team},
            date = {22 June 2017},
            title = {FASTA Database Validation Tool}
            }
        </citation>
    </citations>
</tool>