Mercurial > repos > cmonjeau > stacks
diff STACKS_sort_read_pairs.xml @ 0:d6ba40f6c824
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author | cmonjeau |
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date | Mon, 24 Aug 2015 09:29:12 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/STACKS_sort_read_pairs.xml Mon Aug 24 09:29:12 2015 +0000 @@ -0,0 +1,164 @@ +<tool id="STACKSassembleperead" name="STACKS : assemble read pairs by locus" version="1.1.1"> + <description>Run the STACKS sort_read_pairs.pl and exec_velvet.pl wrappers</description> + +<requirements> + <requirement type="package" version="1.18">stacks</requirement> + <requirement type="package" version="1.2.10">velvet</requirement> + </requirements> +<command interpreter="python"> + +STACKS_sort_read_pairs.py +-a $STACKS_archive +-e $samples_archive +#if str( $options_whitelist.whitelist_selector) == "yes" +-b $whitelist +#end if +#if str( $options_filter.reads_selector) == "yes" +-c $options_filter.threshold +#end if +#if str( $options_filter2.length_selector) == "yes" +-d $options_filter2.threshold2 +#end if +-o $output + + +</command> + +<inputs> +<param name="STACKS_archive" format="zip,tar.gz" type="data" label="Archive from STACKS pipeline regrouping all outputs" /> + +<param name="samples_archive" format="zip,fastq.gz,tar.gz,tar.bz2" type="data" label="Archive with raw reads used to execute previous STACKS pipeline" /> + + <conditional name="options_whitelist"> +<param name="whitelist_selector" type="select" label="Have you got a whitelist?" > +<option value="no" selected="true">No</option> +<option value="yes">Yes</option> +</param> +<when value="no"></when> +<when value="yes"> +<param name="whitelist" format="txt, tabular" type="data" label="Whitelist file containing loci that we want to assemble: those that have SNPs" /> +</when> +</conditional> + + +<conditional name="options_filter"> +<param name="reads_selector" type="select" label="Specify a treshold for the minimum number of reads by locus?" > +<option value="no" selected="true">No</option> +<option value="yes">Yes</option> +</param> +<when value="no"></when> +<when value="yes"> +<param name="threshold" type="integer" value="10" label="Minimum number of reads by locus"/> +</when> +</conditional> +<conditional name="options_filter2"> +<param name="length_selector" type="select" label="Specify a minimum length for asssembled contigs?" > +<option value="no" selected="true">No</option> +<option value="yes">Yes</option> +</param> +<when value="no"></when> +<when value="yes"> +<param name="threshold2" type="integer" value="200" label="Minimum length for asssembled contigs"/> +</when> +</conditional> + +</inputs> +<outputs> + <data format="fasta" name="output" label="collated.fa : ${tool.name} on ${on_string}" /> +</outputs> +<stdio> + <exit_code range="1" level="fatal" description="Error" /> +</stdio> +<help> + +.. class:: infomark + +**What it does** + +This program will run each of the Stacks sort_read_pairs.pl and exec_velvet.pl utilities to assemble pair-end reads from STACKS pipeline results + +-------- + +**Created by:** + +Stacks was developed by Julian Catchen with contributions from Angel Amores, Paul Hohenlohe, and Bill Cresko + +-------- + +**Example:** + +Input file: + +Output archives of STACKS : Reference map or STACKS : De novo map, in zip or tar.gz format + + +Output file: + +A collated.fa file containing assembled contigs for each locus + + +Instructions to add the functionality of archives management in Galaxy on the `eBiogenouest HUB wiki <https://www.e-biogenouest.org/wiki/ManArchiveGalaxy>`_ . + +-------- + + +**Project links:** + +`STACKS website <http://creskolab.uoregon.edu/stacks/>`_ . + +`STACKS manual <http://creskolab.uoregon.edu/stacks/stacks_manual.pdf>`_ . + +`STACKS google group <https://groups.google.com/forum/#!forum/stacks-users>`_ . + +-------- + +**References:** + +-J. Catchen, P. Hohenlohe, S. Bassham, A. Amores, and W. Cresko. Stacks: an analysis tool set for population genomics. Molecular Ecology. 2013. + +-J. Catchen, S. Bassham, T. Wilson, M. Currey, C. O'Brien, Q. Yeates, and W. Cresko. The population structure and recent colonization history of Oregon threespine stickleback determined using restriction-site associated DNA-sequencing. Molecular Ecology. 2013. + +-J. Catchen, A. Amores, P. Hohenlohe, W. Cresko, and J. Postlethwait. Stacks: building and genotyping loci de novo from short-read sequences. G3: Genes, Genomes, Genetics, 1:171-182, 2011. + +-A. Amores, J. Catchen, A. Ferrara, Q. Fontenot and J. Postlethwait. Genome evolution and meiotic maps by massively parallel DNA sequencing: Spotted gar, an outgroup for the teleost genome duplication. Genetics, 188:799'808, 2011. + +-P. Hohenlohe, S. Amish, J. Catchen, F. Allendorf, G. Luikart. RAD sequencing identifies thousands of SNPs for assessing hybridization between rainbow trout and westslope cutthroat trout. Molecular Ecology Resources, 11(s1):117-122, 2011. + +-K. Emerson, C. Merz, J. Catchen, P. Hohenlohe, W. Cresko, W. Bradshaw, C. Holzapfel. Resolving postglacial phylogeography using high-throughput sequencing. Proceedings of the National Academy of Science, 107(37):16196-200, 2010. + +-------- + +**Integrated by:** + +Yvan Le Bras and Cyril Monjeaud + +GenOuest Bio-informatics Core Facility + +UMR 6074 IRISA INRIA-CNRS-UR1 Rennes (France) + +support@genouest.org + +If you use this tool in Galaxy, please cite : + +`Y. Le Bras, A. Roult, C. Monjeaud, M. Bahin, O. Quenez, C. Heriveau, A. Bretaudeau, O. Sallou, O. Collin, Towards a Life Sciences Virtual Research Environment : an e-Science initiative in Western France. JOBIM 2013. <https://www.e-biogenouest.org/resources/128>`_ + + +</help> +<citations> + <citation type="doi">10.1111/mec.12354</citation> + <citation type="doi">10.1111/mec.12330</citation> + <citation type="doi">10.1534/g3.111.000240</citation> + <citation type="doi">10.1534/genetics.111.127324</citation> + <citation type="doi">10.1111/j.1755-0998.2010.02967.x</citation> + <citation type="doi">10.1073/pnas.1006538107</citation> + + <citation type="bibtex">@INPROCEEDINGS{JOBIM2013, + author = {Le Bras, Y. and ROULT, A. and Monjeaud, C. and Bahin, M. and Quenez, O. and Heriveau, C. and Bretaudeau, A. and Sallou, O. and Collin, O.}, + title = {Towards a Life Sciences Virtual Research Environment: An e-Science initiative in Western France}, + booktitle = {JOBIM 2013 Proceedings}, + year = {2013}, + url = {https://www.e-biogenouest.org/resources/128}, + pages = {97-106} + }</citation> +</citations> +</tool>