Mercurial > repos > cpt > cpt_linear_genome_plot
annotate dna_features_viewer/biotools.py @ 6:e2449c8659be draft default tip
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author | cpt |
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date | Sat, 13 Jul 2024 20:35:48 +0000 |
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1 import textwrap |
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2 from Bio.Seq import Seq |
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3 from Bio.SeqFeature import SeqFeature, FeatureLocation |
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4 from Bio.PDB.Polypeptide import aa1, aa3 |
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5 from Bio import SeqIO |
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6 |
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7 try: |
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8 from BCBio import GFF |
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9 except ImportError: |
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10 |
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11 class GFF: |
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12 def parse(*a): |
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13 """Not available. Please install bcbio-gff.""" |
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14 raise ImportError("Please install the bcbio-gff library to parse GFF data") |
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15 |
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16 |
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17 def complement(dna_sequence): |
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18 """Return the complement of the DNA sequence. |
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19 |
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20 For instance ``complement("ATGCCG")`` returns ``"TACGGC"``. |
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21 |
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22 Uses BioPython for speed. |
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23 """ |
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24 return str(Seq(dna_sequence).complement()) |
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25 |
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26 |
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27 def reverse_complement(sequence): |
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28 """Return the reverse-complement of the DNA sequence. |
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29 |
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30 For instance ``complement("ATGCCG")`` returns ``"GCCGTA"``. |
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31 |
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32 Uses BioPython for speed. |
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33 """ |
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34 return complement(sequence)[::-1] |
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35 |
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36 |
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37 if type(aa1) is str and type(aa3) is list: |
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38 aa_short_to_long_form_dict = { |
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39 _aa1: _aa3[0] + _aa3[1:].lower() for (_aa1, _aa3) in zip(aa1 + "*", aa3 + ["*"]) |
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40 } |
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41 else: |
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42 aa_short_to_long_form_dict = { |
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43 _aa1: _aa3[0] + _aa3[1:].lower() |
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44 for (_aa1, _aa3) in zip(aa1 + ("*",), aa3 + ("*",)) |
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45 } |
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46 |
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47 |
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48 def translate(dna_sequence, long_form=False): |
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49 """Translate the DNA sequence into an amino-acids sequence MLKYQT... |
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50 |
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51 If long_form is true, a list of 3-letter amino acid representations |
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52 is returned instead (['Ala', 'Ser', ...]). |
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53 """ |
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54 result = str(Seq(dna_sequence).translate()) |
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55 if long_form: |
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56 result = [aa_short_to_long_form_dict[aa] for aa in result] |
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57 return result |
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58 |
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59 |
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60 def extract_graphical_translation(sequence, location, long_form=False): |
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61 """Return a string of the "graphical" translation of a sequence's subsegment. |
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62 |
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63 Here "graphical" means that the amino acid sequence is always given |
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64 left-to-right, as it will appear under the sequence in the plot. This matters |
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65 when the location is on the -1 strand. In this case, the amino-acids are |
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66 determined by (part of) the reverse-complement of the sequence, however |
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67 the sequence returned will be the mirror of the translated sequence, as |
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68 this is the left-to-right order in which the codons corresponding to the |
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69 amino-acids appear in the sequence. |
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70 |
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71 Parameters |
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72 ---------- |
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73 sequence |
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74 An "ATGC" string. |
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75 |
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76 location |
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77 Either (start, end) or (start, end, strand), with strand in (0, 1, -1). |
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78 |
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79 long_form |
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80 if True, a list of 3-letter amino acid representations is returned instead |
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81 (['Ala', 'Ser', ...]). |
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82 |
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83 """ |
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84 if len(location) == 3: |
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85 start, end, strand = location |
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86 else: |
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87 start, end = location |
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88 strand = 1 |
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89 subsequence = sequence[start:end] |
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90 if strand == -1: |
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91 subsequence = reverse_complement(subsequence) |
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92 translation = translate(subsequence, long_form=long_form) |
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93 if strand == -1: |
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94 translation = translation[::-1] |
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95 return translation |
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96 |
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97 |
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98 def load_record(path): |
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99 """Load a Genbank file""" |
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100 if isinstance(path, str): |
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101 # Input is a file path |
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102 if path.lower().endswith(".gff"): |
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103 return list(GFF.parse(path))[0] |
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104 else: |
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105 return SeqIO.read(path, "genbank") |
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106 else: |
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107 # Input is a file-like object |
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108 try: |
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109 return SeqIO.read(path, "genbank") |
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110 except: |
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111 path.seek(0) |
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112 return list(GFF.parse(path))[0] |
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113 |
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114 |
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115 def annotate_biopython_record( |
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116 seqrecord, location="full", feature_type="misc_feature", margin=0, **qualifiers |
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117 ): |
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118 """Add a feature to a Biopython SeqRecord. |
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119 |
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120 Parameters |
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121 ---------- |
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122 |
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123 seqrecord |
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124 The biopython seqrecord to be annotated. |
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125 |
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126 location |
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127 Either (start, end) or (start, end, strand). (strand defaults to +1) |
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128 |
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129 feature_type |
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130 The type associated with the feature |
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131 |
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132 margin |
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133 Number of extra bases added on each side of the given location. |
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134 |
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135 qualifiers |
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136 Dictionnary that will be the Biopython feature's `qualifiers` attribute. |
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137 """ |
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138 if location == "full": |
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139 location = (margin, len(seqrecord) - margin) |
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140 |
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141 strand = location[2] if len(location) == 3 else 1 |
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142 seqrecord.features.append( |
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143 SeqFeature( |
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144 FeatureLocation(location[0], location[1], strand), |
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145 qualifiers=qualifiers, |
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146 type=feature_type, |
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147 ) |
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148 ) |
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149 |
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150 |
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151 def find_narrowest_text_wrap(text, max_line_length): |
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152 """Wrap the text into a multi-line text minimizing the longest line length. |
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153 |
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154 This is done by first wrapping the text using max_line_length, then |
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155 attempt new wraps by iteratively decreasing the line_length, as long as the |
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156 number of lines stays the same as with max_line_length. |
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157 """ |
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158 narrowest_wrap = textwrap.wrap(text, max_line_length) |
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159 narrowest_width = max([len(l) for l in narrowest_wrap]) |
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160 for line_length in range(max_line_length - 1, 0, -1): |
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161 wrap = textwrap.wrap(text, line_length) |
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162 if len(wrap) <= len(narrowest_wrap): |
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163 width = max([len(l) for l in wrap]) |
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164 if width < narrowest_width: |
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165 narrowest_wrap = wrap |
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166 narrowest_width = width |
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167 else: |
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168 break |
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169 return "\n".join(narrowest_wrap) |