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LOCUS Exported 36717 bp ds-DNA linear PHG 03-JUN-2020 DEFINITION Enterobacteria phage Mu, complete genome. ACCESSION NC_000929 VERSION . KEYWORDS . SOURCE Escherichia phage Mu ORGANISM Escherichia phage Mu REFERENCE 1 (bases 1 to 36717) AUTHORS Morgan GJ, Hatfull GF, Casjens S, Hendrix RW. TITLE Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus JOURNAL J. Mol. Biol. 317 (3), 337-359 (2002) PUBMED 11922669 REFERENCE 2 (bases 1 to 36717) TITLE Direct Submission JOURNAL Submitted (05-MAY-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 36717) AUTHORS Morgan G, Hatfull G, Hendrix R. TITLE Direct Submission JOURNAL Submitted (13-AUG-1998) Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA REFERENCE 4 (bases 1 to 36717) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Jun 3, 2020 from SnapGene 5.1.3 https://www.snapgene.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. Biol."; date: "2002"; volume: "317"; issue: "3"; pages: "337-359" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (05-MAY-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (13-AUG-1998) Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA" COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF083977. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..36717 /organism="Escherichia phage Mu" /host="Escherichia coli" /mol_type="genomic DNA" /label=G(+) form /note="G(+) form" /db_xref="taxon:2681603" gene complement(339..942) /locus_tag="Mup01" /label=Mup01 /db_xref="GeneID:2636266" CDS complement(339..929) /codon_start=1 /transl_table=11 /locus_tag="Mup01" /product="repressor protein c" /label=Mup01 /note="Mup01 or immunity repressor or MuR for repressor of replication or Rep c; see PMIDs 16154589 and 9546656, 11135677 for structure; contains InterPro domain IPR003314 Mu-type HTH domain also found in Mu transposase (Mup03 or A)" /db_xref="GeneID:2636266" /protein_id="NP_050605.1" /translation="MAADGMPGSVAGVHYRANVQGWTKQKKEGVKGGKAVEYDVMSMPT KEREQVIAHLGLSTPDTGAQANEKQDSSELINKLTTTLINMIEELEPDEARKALKLLSK GGLLALMPLVFNEQKLYSFIGFSQQSIQTLMMLDALPEEKRKEILSKYGIHEQESVVVP SQEPQEVKKAV" RBS complement(938..942) /locus_tag="Mup01" gene 1085..1326 /locus_tag="Mup02" /label=Mup02 /db_xref="GeneID:2636289" RBS 1085..1090 /locus_tag="Mup02" CDS 1099..1326 /codon_start=1 /transl_table=11 /locus_tag="Mup02" /product="DNA binding protein ner" /label=Mup02 /note="negative regulator of early transcription; see PMIDs 6293190, 6096223, 3015876; contains InterPro domain IPR038722 Ner, winged helix-turn-helix DNA-binding domain" /db_xref="GeneID:2636289" /protein_id="NP_050606.1" /translation="MCSNEKARDWHRADVIAGLKKRKLSLSALSRQFGYAPTTLANALE RHWPKGEQIIANALETKPEVIWPSRYQAGE" gene 1317..3319 /locus_tag="Mup03" /label=Mup03 /db_xref="GeneID:2636292" RBS 1317..1320 /locus_tag="Mup03" CDS 1328..3319 /codon_start=1 /transl_table=11 /locus_tag="Mup03" /product="transposase" /label=Mup03 /note="DDE-recombinase A or MuA or A; see PMID 1655409 and many others; contains InterPro domains IPR003314, IPR015126, IPR004189, IPR015378 which are Mu-type HTH domain, Mu DNA binding I gamma subdomain, and transposase domains" /db_xref="GeneID:2636292" /protein_id="NP_050607.1" /translation="MELWVSPKECANLPGLPKTSAGVIYVAKKQGWQNRTRAGVKGGKA IEYNANSLPVEAKAALLLRQGEIETSLGYFEIARPTLEAHDYDREALWSKWDNASDSQR RLAEKWLPAVQAADEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWAAA LVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIPSRA TAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFVRWFN GDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYGIPEDFHITIDNTRG AANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVERAFGVGG LEEYVDKHPALAGAYTGPNPQAKPDNYGDRAVDAELFLKTLAEGVAMFNARTGRETEMC GGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGGSLKGAKNV YYNMALMNAGVKKVVVRFDPQQLHSTVYCYTLDGRFICEAECLAPVAFNDAAAGREYRR RQKQLKSATKAAIKAQKQMDALEVAELLPQIAEPAAPESRIVGIFRPSGNTERVKNQER DDEYETERDEYLNHSLDILEQNRRKKAI" gene 3346..4296 /locus_tag="Mup04" /label=Mup04 /db_xref="GeneID:2636257" RBS 3346..3350 /locus_tag="Mup04" CDS 3358..4296 /codon_start=1 /transl_table=11 /locus_tag="Mup04" /product="AAA-ATPase DNA transposition protein" /label=Mup04 /note="B or MuB or ATP-dependent target DNA activator B; see PMIDs 1646076, 11060014, 23776210, 12791691 and many more; contains InterPro domains IPR003593 and IPR009084 for AAA+ ATPase domain and B transposition protein C-terminal region" /db_xref="GeneID:2636257" /protein_id="NP_050608.1" /translation="MNISDIRAGLRTLVENEETTFKQIALESGLSTGTISSFINDKYNG DNERVSQMLQRWLEKYHAVAELPEPPRFVETQTVKQIWTSMRFASLTESIAVVCGNPGV GKTEAAREYRRTNNNVWMITITPSCASVLECLTELAFELGMNDAPRRKGPLSRALRRRL EGTQGLVIIDEADHLGAEVLEELRLLQESTRIGLVLMGNHRVYSNMTGGNRTVEFARLF SRIAKRTAINKTKKADVKAIADAWQINGEKELELLQQIAQKPGALRILNHSLRLAAMTA HGKGERVNEDYLRQAFRELDLDVDISTLLRN" gene 4299..4539 /locus_tag="Mup05" /label=Mup05 /db_xref="GeneID:2636299" RBS 4299..4303 /locus_tag="Mup05" CDS 4315..4539 /codon_start=1 /transl_table=11 /locus_tag="Mup05" /product="protein kil" /label=Mup05 /note="Mup05 also known as cim for control of immunity; see PMIDs 6234699, 2531953, 6237246" /db_xref="GeneID:2636299" /protein_id="NP_050609.1" /translation="MARNIKMATDAQNWLQARGSHVNESYLGVARPILEITYPPVELVK NAVRIMEHKSGVARSVWTARLNGCQIIWR" gene 4532..4772 /locus_tag="Mup06" /label=Mup06 /db_xref="GeneID:2636264" RBS 4532..4535 /locus_tag="Mup06" CDS 4542..4772 /codon_start=1 /transl_table=11 /locus_tag="Mup06" /product="uncharacterized protein" /label=Mup06 /note="gp6; previously called E5" /db_xref="GeneID:2636264" /protein_id="NP_050610.1" /translation="MCIKAEKYIEWVKHCQCHGVPLTTYKCPGCGEQIMTQCSPEKEIR DSLTCCPWCSAVFFKQVKGAKVKASAVIQNQ" gene 4772..5047 /locus_tag="Mup07" /label=Mup07 /db_xref="GeneID:2636268" RBS 4772..4775 /locus_tag="Mup07" CDS 4784..5047 /codon_start=1 /transl_table=11 /locus_tag="Mup07" /product="uncharacterized protein" /label=Mup07 /note="gp7; previously called E6" /db_xref="GeneID:2636268" /protein_id="NP_050611.1" /translation="MAKVIIEIKNTVSGIKGRNLRTSIAVDGSAELDGDEGTLAGMVAL LVLNKSQKIINESAHEAIEILKNDGVITSGRVTEMAVEKTCH" gene 5049..5481 /locus_tag="Mup08" /label=Mup08 /db_xref="GeneID:2636279" RBS 5049..5052 /locus_tag="Mup08" CDS 5062..5481 /codon_start=1 /transl_table=11 /locus_tag="Mup08" /product="uncharacterized protein" /label=Mup08 /note="gp8; previously called E7" /db_xref="GeneID:2636279" /protein_id="NP_050612.1" /translation="MNVKIRNEIQALIRIQERNNNGGELREFICAREVDGYGEKTYLIT FDHYSICARYCGESISRAIASGDAFNVDLWEYVMDREYICASDPEAREMWQRIWRDYRL MAKGWARCCYSSLALKAVQLSLRHIPASLREPLLY" gene 5484..5781 /locus_tag="Mup09" /label=Mup09 /db_xref="GeneID:2636278" RBS 5484..5486 /locus_tag="Mup09" CDS 5494..5781 /codon_start=1 /transl_table=11 /locus_tag="Mup09" /product="uncharacterized protein" /label=Mup09 /note="gp9; previously called E8" /db_xref="GeneID:2636278" /protein_id="NP_050613.1" /translation="MVTDMKCNRKRWSREDREFIEANVGKMTVEEMAEKLKVATTALRA HARRHGISLCVYRISEHDKYLCRELYKEGLDIHVIARKMELSNRAVSSIVYSGY" gene 5787..6325 /locus_tag="Mup10" /label=Mup10 /db_xref="GeneID:2636288" RBS 5787..5791 /locus_tag="Mup10" CDS 5801..6325 /codon_start=1 /transl_table=11 /locus_tag="Mup10" /product="host nuclease inhibitor Gam" /label=Mup10 /note="inhibition of exonuclease digestion of Mu DNA; see PMIDs 2945162 and 12524520" /db_xref="GeneID:2636288" /protein_id="NP_050614.1" /translation="MAKPAKRIKSAAAAYVPQNRDAVITDIKRIGDLQREASRLETEMN DAIAEITEKFAARIAPIKTDIETLSKGVQGWCEANRDELTNGGKVKTANLVTGDVSWRV RPPSVSIRGMDAVMETLERLGLQRFIRTKQEINKEAILLEPKAVAGVAGITVKSGIEDF SIIPFEQEAGI" gene 6412..6954 /locus_tag="Mup11" /label=Mup11 /db_xref="GeneID:2636297" RBS 6412..6415 /locus_tag="Mup11" CDS 6424..6954 /codon_start=1 /transl_table=11 /locus_tag="Mup11" /product="uncharacterized protein" /label=Mup11 /note="gp11; combines what was previously E10, E11, andE12; those seqeuces either have frameshift sequencing errors or there is strain variation" /db_xref="GeneID:2636297" /protein_id="NP_050615.1" /translation="MVDAKILNGVSTLLRAYGRLTCGVLAEKMNMLPSSMVYFLRDAVD AGVLTECNGFYDVPRPRPTPPVRRNATEQPAVDDAVWCNWRRSLPWVEGNTIPALAKEF ATGVLTCESVHIVAEVDNRMCEQGMPRFVMAYIDIRLGRFICSSSVWNITDHVLRYLIL DCSPAPAAVQEVA" gene 6941..7484 /locus_tag="Mup12" /label=Mup12 /db_xref="GeneID:2636294" RBS 6941..6947 /locus_tag="Mup12" CDS 6954..7484 /codon_start=1 /transl_table=11 /locus_tag="Mup12" /product="uncharacterized protein" /label=Mup12 /note="gp12; previously called E13" /db_xref="GeneID:2636294" /protein_id="NP_050616.1" /translation="MFFKTSNPAALLAWDQFMADCLKLREEARHLDKVLGCGCRSVFST SIGGRYFHGVNFPGNERPFSRELWTVQRPASGNSCRPRTSRIPAHLREQARELAKIWQE NIPVTYARTDALLPALGLDFSATIFGPLQWFRVGDVIYVMTGMTPAQGRMTEILSDEFI RAQKQAEVNNGKQ" gene 7460..7653 /locus_tag="Mup13" /label=Mup13 /db_xref="GeneID:2636290" RBS 7460..7465 /locus_tag="Mup13" CDS 7471..7653 /codon_start=1 /transl_table=11 /locus_tag="Mup13" /product="uncharacterized protein" /label=Mup13 /note="gp13; previously called E14" /db_xref="GeneID:2636290" /protein_id="NP_050617.1" /translation="MENNKTSYSWLGKFTTVKQECPTCGNESPEYLKECPHCGGLKCNH CDMGDDTACMNCEGE" gene 7644..7957 /locus_tag="Mup14" /label=Mup14 /db_xref="GeneID:2636262" RBS 7644..7647 /locus_tag="Mup14" CDS 7655..7957 /codon_start=1 /transl_table=11 /locus_tag="Mup14" /product="uncharacterized protein" /label=Mup14 /note="gp14; previously called E15 which has an different earlier start site" /db_xref="GeneID:2636262" /protein_id="NP_050618.1" /translation="MNNETKFTPLNIDNVMAEKGMLERVRAIVEYGIKHNLTAREVRDI INREMNRLETVVALQNETAREEYIRRRLGLSDQDIVTDAHVFEAFEIRQHLGLTN" gene complement(7896..8171) /locus_tag="Mup15" /label=Mup15 /db_xref="GeneID:2636284" CDS complement(7896..8162) /codon_start=1 /transl_table=11 /locus_tag="Mup15" /product="uncharacterized protein" /note="overlaps a potential coding sequence on opposite strand" /note="gp15" /label=Mup15 /db_xref="GeneID:2636284" /protein_id="NP_050619.1" /translation="MENNVQPYDVAGYAIASALVRLLVKKAIITAEEGKAIFSSSAEIL KDAPAMRTSRREKLQLSKIMEDIISSLDPDADGSQKPQTHERQ" gene 7943..8127 /locus_tag="Mup15r" RBS 7943..7945 /locus_tag="Mup15r" CDS 7960..8127 /locus_tag="Mup15r" /product="hypothetical protein" /note="unsure, but this is a plus strand protein between 14 and 16 that could replace the 15 that overlaps 14 in the opposite orientation" /transl_table=11 /translation="MSSMIFDSCSFSRRLVLIAGASFKISAEELKMALPSSAVMIAFF TRRRTSADAIA" RBS complement(8168..8171) /locus_tag="Mup15" gene 8221..8784 /locus_tag="Mup16" /label=Mup16 /db_xref="GeneID:2636256" RBS 8221..8226 /locus_tag="Mup16" CDS 8233..8784 /codon_start=1 /transl_table=11 /locus_tag="Mup16" /product="protein GemA" /label=Mup16 /note="gp16; also called E16 and GemA for gene expression modulation; see PMIDs 2543906 and 11278077" /db_xref="GeneID:2636256" /protein_id="NP_050620.1" /translation="MSRTSLIKLIHVARRELQLDDDTYRAFLMQKTGKISCRELTVTQL EQVLGAMKERGFKKQNKYPRRRFKGHVTPREKVYKIWQQMAEDGFITDGGDVALDKYVQ RLTAKRNGGQGVSTLAWCHGDTLLTVLETLKQWHIRCIREAFSRHGLPLPVSPSGRELR GYDAMTAAYAHARKTRRMAQ" gene 8768..9170 /locus_tag="Mup17" /label=Mup17 /db_xref="GeneID:2636272" RBS 8768..8772 /locus_tag="Mup17" CDS 8781..9170 /codon_start=1 /transl_table=11 /locus_tag="Mup17" /product="middle operon regulator Mor" /label=Mup17 /note="Mor, positive regulator of the middle operon, also called E17 and formerly gemB; see PMIDs 2173258, 8790343 and 9454718 and structures in PMIDs 14729670 and 21859715" /db_xref="GeneID:2636272" /protein_id="NP_050621.1" /translation="MTEDLFGDLQDDTILAHLDNPAEDTSRFPALLAELNDLLRGELSR LGVDPAHSLEIVVAICKHLGGGQVYIPRGQALDSLIRDLRIWNDFNGRNVSELTTRYGV TFNTVYKAIRRMRRLKYRQYQPSLL" gene 9235..9466 /locus_tag="Mup18" /label=Mup18 /db_xref="GeneID:2636281" RBS 9235..9238 /locus_tag="Mup18" CDS 9248..9466 /codon_start=1 /transl_table=11 /locus_tag="Mup18" /product="uncharacterized protein" /label=Mup18 /note="E18" /db_xref="GeneID:2636281" /protein_id="NP_050622.1" /translation="MGKGWNASFHLGRRERLRQEVLHRVAGGPRPAPRDYTGHDGTHGS YYMKGWQSVDMPEILHHCLLYREKHYV" gene 9448..9821 /locus_tag="Mup19" /label=Mup19 /db_xref="GeneID:2636295" RBS 9448..9451 /locus_tag="Mup19" CDS 9459..9821 /codon_start=1 /transl_table=11 /locus_tag="Mup19" /product="pinholin" /label=Mup19 /note="E19; N-in C-in 4 transmembrane domain protein predicted by Phobius and TMHMM" /db_xref="GeneID:2636295" /protein_id="NP_050623.1" /translation="MSERSARQWPDFLSVVLLALLLWISLFCGWRALMFCCASVFSVAL CVAADCLDALIMSCRVPEHFARFVWPLTWLGSLSGLGLAVMATSQLKTGPEHVIWALAG LLTFWLSFRFRARLFG" gene 9816..9950 /locus_tag="Mup20" /label=Mup20 /db_xref="GeneID:2636298" RBS 9816..9819 /locus_tag="Mup20" CDS 9831..9950 /codon_start=1 /transl_table=11 /locus_tag="Mup20" /product="antiholin" /label=Mup20 /note="gp20; N-in C-out 1 transmembrane domain predicted by Phobius and TMHMM" /db_xref="GeneID:2636298" /protein_id="NP_050624.1" /translation="MYRKFSDECFGPSTLINAIKVIALVVLITISAVVYLSVC" gene 9953..10385 /locus_tag="Mup21" /label=Mup21 /db_xref="GeneID:2636301" RBS 9953..9957 /locus_tag="Mup21" CDS 9963..10385 /codon_start=1 /transl_table=11 /locus_tag="Mup21" /product="transcription regulator C" /label=Mup21 /note="C late protein transcriptional activator; contains InterPro domain IPR014875 for the Mor transcription activator" /note="/color=192 192 192" /db_xref="GeneID:2636301" /protein_id="NP_050625.1" /translation="MQHDLFEHDPAIRQLIGHIDNIPAPELESRWPRSVVDLIDVLENE LKRQNVSNPRELARKQAVALSCFLGGRQFYIPCGDTILTALRDDLLYCQFNGRNMEELR RQYRLSQPQIYQIIARQRKLHTRRHQPDLFSPETPK" gene 10469..10995 /locus_tag="Mup22" /label=Mup22 /db_xref="GeneID:2636261" RBS 10469..10471 /locus_tag="Mup22" CDS 10477..10995 /codon_start=1 /transl_table=11 /locus_tag="Mup22" /product="SAR endolysin" /label=Mup22 /note="Lys gp22; catalytic residues E37, D46, T52; N-terminal signal-arrest-release domain; see PMID 4576742; contains InterPro domain IPR002196 for lysozyme of the Glycoside hydrolase family 24" /db_xref="GeneID:2636261" /protein_id="NP_050626.1" /translation="MMAGIPKKLKAALLAVTIAGGGVGGYQEMTRQSLIHLENIAYMPYR DIAGVLTVCVGHTGPDIEMRRYSHAECMALLDSDLKPVYAAIDRLVRVPLTPYQKTALA TFIFNTGVTAFSKSTLLKKLNAGDYAGARDQMARWVFAAGHKWKGLMNRREVEMAIWNI RGADDLRQ" gene 10970..11365 /locus_tag="Mup23" /label=Mup23 /db_xref="GeneID:2636265" RBS 10970..10973 /locus_tag="Mup23" CDS 10979..11365 /codon_start=1 /transl_table=11 /locus_tag="Mup23" /product="i-spanin" /label=Mup23 /note="gp23 inner membrane spanin subunit; 1 transmembrane domain N-in C-out" /db_xref="GeneID:2636265" /protein_id="NP_050627.1" /translation="MTFASKSLLLAAVFTAVLSGGLWHRLDSTRHDNQTLRRELQTEQQ ARHTAEWLLHGQEQTMQVFSAIRAANRAARLADETEHHDAKEKITTAITGDNCSTRPVP AVAADRLRELEKRTRAIGGDPARN" gene 11091..11567 /locus_tag="Mup23a" misc_feature 11159 /locus_tag="Mup23" /label=possible alternate start site for gp23 /note="possible alternate start site for gp23" RBS 11213..11215 /locus_tag="Mup23a" CDS 11226..11567 /codon_start=1 /locus_tag="Mup23a" /transl_table=11 /product="o-spanin" /label=gp23a /note="outer membrane spanin subunit; lipoylation site predicted at C29 with lipobox LTGC predicted by PROSITE PS51257" /translation="MMQKKKLQPPLLVTIAALVLCLPLLLTGCGNSKNAPVPSVVILPE IDTELTEATPVPPMPQPLTWGASLLWNADLLMALGQCNRDKASVREQEIRRKEIYERRP EPGGGAAAR" gene 11511..11718 /locus_tag="Mup24" /label=Mup24 /db_xref="GeneID:2636252" RBS 11511..11514 /locus_tag="Mup24" CDS 11524..11718 /codon_start=1 /transl_table=11 /locus_tag="Mup24" /product="uncharacterized protein" /label=Mup24 /note="gp24; HHPred hit with 93 probability over all of query and most of target to PDB entry 2KGO E. coli YbiL UniProt P41039" /db_xref="GeneID:2636252" /protein_id="NP_050628.1" /translation="MNEDQNRAVALLLAELWQGDTRDIPRPAAYDPPVLCAGCGRELRP DVLRQQPMANYCHWCRGAE" gene 11707..12017 /locus_tag="Mup25" /label=Mup25 /db_xref="GeneID:2636263" RBS 11707..11710 /locus_tag="Mup25" CDS 11718..12017 /codon_start=1 /transl_table=11 /locus_tag="Mup25" /product="releasin" /label=Mup25 /note="gp25; contains InterPro domain IPR020269 domain of unknown function 2730; N-out C-in 1 transmembrane domain predicted by TMHMM" /db_xref="GeneID:2636263" /protein_id="NP_050629.1" /translation="MDLISVLALWPYLLPVVAGGAVWAMRRSFASTERVERLENRMTEM ETRYASIPGTEDVHEMRLRIAELSGDIRVLSQRVQSFSHQLELLLENAVNRSNS" gene 12004..12304 /locus_tag="Mup26" /label=Mup26 /db_xref="GeneID:2636255" RBS 12004..12007 /locus_tag="Mup26" CDS 12014..12304 /codon_start=1 /transl_table=11 /locus_tag="Mup26" /product="winged HTH domain-containing protein" /label=Mup26 /note="gp26; contains InterPro entry IPR036390 for Winged helix DNA-binding domain superfamily" /db_xref="GeneID:2636255" /protein_id="NP_050630.1" /translation="MINDILTEDRRLVILRSLMDCNNEANESILQDCLDAYGHNVSRDV VRGQIDWLAEQQLVTVENLRGFYVVTLTSRGQDVAEGRARVAGVKRPRPRA" gene 12306..12891 /locus_tag="Mup27" /label=Mup27 /db_xref="GeneID:2636271" RBS 12306..12310 /locus_tag="Mup27" CDS 12316..12891 /codon_start=1 /transl_table=11 /locus_tag="Mup27" /product="terminase small subunit" /label=Mup27 /note="gp27 or D" /db_xref="GeneID:2636271" /protein_id="NP_050631.1" /translation="MDRKTRGRASKVDLLPENVRKTLHEMLRDKAIPQARILEEINALI EDAGLPDEMKLSRSGLNRYATNVEQVGHNLRQMREMTSALTAELGDKPMGETTKLILEM ARSQLFKAMMRQIENPESDVDIDLLKNAMLAAQRLESTAMSSHRREKEIRQAFAEEAAN AVSEELRGQDGISEELEQRIRDVLLGKA" gene 12890..14554 /locus_tag="Mup28" /label=Mup28 /db_xref="GeneID:2636303" RBS 12890..12892 /locus_tag="Mup28" CDS 12899..14554 /codon_start=1 /transl_table=11 /locus_tag="Mup28" /product="terminase large subunit" /label=Mup28 /note="gp28; formerly known as E; contains InterPro entry G3DSA:3.40.50.300 for P-loop containing nucleotide triphosphate hydrolases" /db_xref="GeneID:2636303" /protein_id="NP_050632.1" /translation="MNTRENNLKALHAPRKINLREEAGLLGVDIVTDIGEAQPRNEPVF LGYQRRWFEDESQICIAEKSRRTGLTWAEAGRNVMTAAKPKRRGGRNVFYVGSRQEMAL EYIAACALFARAFNQLAKADVWEQTFWDSDKKEEILTYMIRFPNSGFKIQALSSRPSNL RGLQGDVVIDEAAFHEALDELLKAAFALNMWGASVRIISTHNGVDNLFNQYIQDAREGR KDYSVHRITLDDAIADGLYRRICYVTNQPWSPEAEKAWRDGLYRNAPNKESADEEYGCI PKKSGGAYLSRVLIEAAMTPARDIPVLRFEAPDDFESLTPQMRHGIVQDWCEQELLPLL DALSPLNKHVLGEDFARRGDLTVFVPLAITPDLRKRECFRVELRNVTYDQQRQILLFIL SRLPRFTGAAFDATGNGGYLAEAARLIYGPEMIDCISLTPAWYQEWMPKLKGEFEAQNI TIARHQTTLDDLLHIKVDKGIPQIDKGRTKDEGGKGRRHGDFAVALCMAVRASYMNGFV IDEDSIQALPPRHRGDDVDNDDFDDYHQFERGGW" gene 14543..16092 /locus_tag="Mup29" /label=Mup29 /db_xref="GeneID:2636304" RBS 14543..14547 /locus_tag="Mup29" CDS 14554..16092 /codon_start=1 /transl_table=11 /locus_tag="Mup29" /product="portal protein" /label=Mup29 /note="gp29 or H" /db_xref="GeneID:2636304" /protein_id="NP_050633.1" /translation="MGRILDISGQPFDFDDEMQSRSDELAMVMKRTQEHPSSGVTPNRA AQMLRDAERGDLTAQADLAFDMEEKDTHLFSELSKRRLAIQALEWRIAPARDASAQEKK DADMLNEYLHDAAWFEDALFDAGDAILKGYSMQEIEWGWLGKMRVPVALHHRDPALFCA NPDNLNELRLRDASYHGLELQPFGWFMHRAKSRTGYVGTNGLVRTLIWPFIFKNYSVRD FAEFLEIYGLPMRVGKYPTGSTNREKATLMQAVMDIGRRAGGIIPMGMTLDFQSAADGQ SDPFMAMIGWAEKAISKAILGGTLTTEAGDKGARSLGEVHDEVRREIRNADVGQLARSI NRDLIYPLLALNSDSTIDINRLPGIVFDTSEAGDITALSDAIPKLAAGMRIPVSWIQEK LHIPQPVGDEAVFTIQPVVPDNGSQKEAALSAEDIPQEDDIDRMGVSPEDWQRSVDPLL KPVIFSVLKDGPEAAMNKAASLYPQMDDAELIDMLTRAIFVADIWGRLDAAADH" gene 16061..17392 /locus_tag="Mup30" /label=Mup30 /db_xref="GeneID:2636300" RBS 16061..16065 /locus_tag="Mup30" CDS 16073..17392 /codon_start=1 /transl_table=11 /locus_tag="Mup30" /product="minor head protein" /label=Mup30 /note="gp30 or F; contains InterPro domain IPR006528 phage SPP1_gp7 phage head morphogenesis protein SPP1 gp7 family; see PMID 1385991 and 8599204" /db_xref="GeneID:2636300" /protein_id="NP_050634.1" /translation="MPQQTIDLAYAARLPPKEAVAYFRAKGYNITWNWYEQLADAHARA FTVAKATRMDVLTTIREEVERAVSEGITREEFTRTLAPRLQKLGWWGKQIIVDAEGNAK EIELGSPRRLATIYNVNTRTAYGAGRYAQMMNTADLYPYWQYVAVMDGRTRPEHARLHN MVFQYDDIFWQTHYPPNGWNCRCRVRALSAARMKELGLQVSYGASFMNTREVDAGTDES TGEIFRTSSTTFDNGRVKMTPDVGWSYNPGSAAFGTDQALIRKLVEVRDAQLREQVVQT LNNSRERQLAFSLWLKRLAGSRQTGHEIRALGFMTGSVAEAVYQRTGNMPARLLVMNGK SLATTADAALKPEDLQRLPSLMAKPQAVLWDRENHQLLYVVATRDGTARIVVRTSQTVG RQNDRADVLVSISRVSAQSLEAAIADGMIDVLEGHVEVNK" gene 17377..17859 /locus_tag="Mup31" /label=Mup31 /db_xref="GeneID:2636282" RBS 17377..17382 /locus_tag="Mup31" CDS 17389..17859 /codon_start=1 /transl_table=11 /locus_tag="Mup31" /product="capsid morphogenesis protein" /label=Mup31 /note="gp31 or G; contains InterPro domain IPR006522 Phage virion morphogensis protein" /db_xref="GeneID:2636282" /protein_id="NP_050635.1" /translation="MSLDMNVAVDVRRIQLALDELGTVTRDRAIPRVMAAALLSSTEQA FERQADPDTGKGWEAWSDSWLAWRQDHGFVPGSILTLHGDLARSITTDYGQDYALIGSP KIYAAIHQWGGTPDMAPRPAGVPARPYMGLDKTGEQEIFDAIRKRVSAALRQ" misc_feature 17879..17880 /label=gyrase cut site /note="gyrase cut site" gene 18044..19141 /locus_tag="Mup32" /label=Mup32 /db_xref="GeneID:2636253" RBS 18044..18046 /locus_tag="Mup32" CDS 18056..19141 /codon_start=1 /transl_table=11 /locus_tag="Mup32" /product="capsid maturation protease" /label=Mup32 /note="I; contains InterPro domain IPR012106 Protease, Mu phage/prophage I type; gene has potential internal start site for p20 (Z) the postulated scaffold protein -- a structural feature also found in bacteriophage lambda" /db_xref="GeneID:2636253" /protein_id="NP_050636.1" /translation="MKKHAIGIAALNALSIDDDGWCQLLPAGHFSARDGRPFDVTGGQG WFIDGEIAGRLVEGVRALNQDVLIDYEHNQLRKDKGLPPEQLVAAGWFNADEMQWREGE GLFIHPRWTAAAQQRIDDGEFGYLSAVFPYDTATGAVLQIRLAALTNDPGATGMKKLTA LAADLPDILQQENKPMNETLRKLLARLGVTVPENADITDEQATAALTALDTLEINAGKV AALSAELEKAQKAAVDLTKYVPVESYNALRDELAQATAQSATASLSAVLDKAEQEGRIF KSERTYLEQLGGQIGVAALSAQLEKKQPIAALSAMQTTTAKIPSQEKTAVAVLSADEQA AVKALGITEAEYLKMKQEQEK" gene 18576..19141 /locus_tag="Mup33" /label=Mup33 /db_xref="GeneID:2636276" RBS 18576..18580 /locus_tag="Mup33" CDS 18590..19141 /codon_start=1 /transl_table=11 /locus_tag="Mup33" /product="scaffolding protein Z" /label=Mup33 /note="Z, also called p20; see PMIDs 3904174 and 11922669; start site internal to gene I" /db_xref="GeneID:2636276" /protein_id="NP_050637.1" /translation="MNETLRKLLARLGVTVPENADITDEQATAALTALDTLEINAGKVA ALSAELEKAQKAAVDLTKYVPVESYNALRDELAQATAQSATASLSAVLDKAEQEGRIFK SERTYLEQLGGQIGVAALSAQLEKKQPIAALSAMQTTTAKIPSQEKTAVAVLSADEQAA VKALGITEAEYLKMKQEQEK" gene 19125..20055 /locus_tag="Mup34" /label=Mup34 /db_xref="GeneID:2636267" RBS 19125..19128 /locus_tag="Mup34" CDS 19138..20055 /codon_start=1 /transl_table=11 /locus_tag="Mup34" /product="major capsid protein" /label=Mup34 /note="gp34 or T; startsite confirmed by amino acid sequencing" /db_xref="GeneID:2636267" /protein_id="NP_050638.1" /translation="MIVTPASIKALMTSWRKDFQGGLEDAPSQYNKIAMVVNSSTRSNT YGWLGKFPTLKEWVGKRTIQQMEAHGYSIANKTFEGTVGISRDDFEDDNLGIYAPIFQE MGRSAAVQPDELIFKLLKDGFTQPCYDGQNFFDKEHPVYPNVDGTGSAVNTSNIVEQDS FSGLPFYLLDCSRAVKPLIFQERRKPELVARTRIDDDHVFMDNEFLFGASTRRAAGYGF WQMAVAVKGDLTLDNLWKGWQLMRSFEGDGGKKLGLKPTHIVVPVGLEKAAEQLLNREL FADGNTTVSNEMKGKLQLVVADYL" gene 20106..20532 /locus_tag="Mup35" /label=Mup35 /db_xref="GeneID:2636251" RBS 20106..20111 /locus_tag="Mup35" CDS 20122..20532 /codon_start=1 /transl_table=11 /locus_tag="Mup35" /product="head-to-tail connector complex protein" /label=Mup35 /note="gp35; see PMID 29767456; structure of homolog HI1506 from FluMu of H. influenzae structure solved in PMID 17400915" /db_xref="GeneID:2636251" /protein_id="NP_050639.1" /translation="MSGTSLNSQRLDTSRITCTAIIKCLRPVYRRAGIAFTRGENTVEV TEEQLAIIRADSVLSVVSASSAETLAEAGGLDVLGVGDLNTRIRATVAGLDKANPEHFT AGGEPKVKAVSAALGEPVSSAQIKAALAEADA" gene 20518..20954 /locus_tag="Mup36" /label=Mup36 /db_xref="GeneID:2636296" RBS 20518..20521 /locus_tag="Mup36" CDS 20529..20954 /codon_start=1 /transl_table=11 /locus_tag="Mup36" /product="head-to-tail connector complex protein" /label=Mup36 /note="gp36 or J; see PMID 3904174 and structure in PMID 29767456" /db_xref="GeneID:2636296" /protein_id="NP_050640.1" /translation="MNYATVNDLCARYTRTRLDILTRPKTADGQPDDAVAEQALADASA FIDGYLAARFVLPLTVVPSLLKRQCCVVAWFYLNESQPTEQITATYRDTVRWLEQVRDG KTDPGVESRTAASPEGEDLVQVQSDPPVFSRKQKGFI" gene 20943..21502 /locus_tag="Mup37" /label=Mup37 /db_xref="GeneID:2636286" RBS 20943..20946 /locus_tag="Mup37" CDS 20954..21502 /codon_start=1 /transl_table=11 /locus_tag="Mup37" /product="tail terminator protein" /label=Mup37 /note="gp37 or K; see PMID 3904174, 29767456, and 19426744" /db_xref="GeneID:2636286" /protein_id="NP_050641.1" /translation="MLEETEAALLARVRELFGATLRQVEPLTGTWTNEDVHRLFLAPPS VFLAWMGCGEGRTRREVESRWAFFVVAELLNGEPVNRPGIYQIVERLIAGVNGQTFGPT TGMRLTQVRNLCDDNRINAGVVLYGVLFSGTTPLPSVVDLDSLDDYERHWQTWKFPDET PEFAAHINVNQEKDHDAEN" gene 21474..21692 /locus_tag="Mup38" /label=Mup38 /db_xref="GeneID:2636273" RBS 21474..21477 /locus_tag="Mup38" CDS 21489..21692 /codon_start=1 /transl_table=11 /locus_tag="Mup38" /product="putative sheath terminator protein" /label=Mup38 /note="gp38" /db_xref="GeneID:2636273" /protein_id="NP_050642.1" /translation="MLKIKPAAGKAIRDPLTMKLLASEGEEKPRNSFWIRRLAAGDVVE VGSTENTADDTDAAPKKRSKSK" gene 21676..23176 /locus_tag="Mup39" /label=Mup39 /db_xref="GeneID:2636269" RBS 21676..21679 /locus_tag="Mup39" CDS 21689..23176 /codon_start=1 /transl_table=11 /locus_tag="Mup39" /product="tail sheath" /label=Mup39 /note="gp39 or L; startsite confirmed by amino acid sequencing in PMID 9714755; contains InterPro domains IPR035326, IPR035089, IPR020287 which are phage tail sheath domains" /db_xref="GeneID:2636269" /protein_id="NP_050643.1" /translation="MSDISFNAIPSDVRVPLTYIEFDNSNAVSGTPAPRQRVLMFGQSG SKASAAPNVPVRIRSGSQASAAFGQGSMLALMADAFLNANRVAELWCIPQGNGTGNAAV GEISLSGTAGENGSLVTYIAGQRLAVSVAAGATGAALADLLVARIKGQPDLPVTAEVRA DSGDDDTHADVVLSAKFTGALSAVDVRWNYYAGETTPYGIITAFKAASGKNGNPDISAS IAGMGDLQYKYIVMPYTDEPNLNLLRTELQERWGPVNQADGFAVTVLSGTYGDISTFGV SRNDHLISCMGIAGAPEPSYLYAATLCAVASQALSIDPARPLQTLTLPGRMPPAVGDRF TWSERNALLFDGISTFNVNDGGEMQIERMITMYRTNKYGDSDPSYLNVNTIATLSYLRY SLRTRITQKFPNYKLASDGTRFATGQAVVTPSVIKTELLALFEEWENAGLVEDFDTFKE ELYVARNKDDKDRLDVLCGPNLINQFRIFAAQVQFIL" gene 23176..23542 /locus_tag="Mup40" /label=Mup40 /db_xref="GeneID:2636260" RBS 23176..23180 /locus_tag="Mup40" CDS 23186..23542 /codon_start=1 /transl_table=11 /locus_tag="Mup40" /product="tail tube protein" /label=Mup40 /note="M; startsite amino acids determined by protein sequencing from bands of pure tails in PMID: 9714755; also see PMID 29767456; contains InterPro domain IPR019596 bacteriophage Mu GpM tail tube" /db_xref="GeneID:2636260" /protein_id="NP_050644.1" /translation="MAGNQRQGVAFIRVNGMELESMEGASFTPSGITREEVTGSRVYGW KGKPRAAKVECKIPGGGPIGLDEIIDWENITVEFQADTGETWMLANAWQADEPKNDGGE ISLVLMAKQSKRIA" gene 23543..23986 /locus_tag="Mup41" /label=Mup41 /db_xref="GeneID:2636287" gene 23543..24116 /locus_tag="Mup41a" /label=Mup41a /db_xref="GeneID:2636287" RBS 23543..23546 /locus_tag="Mup41" RBS 23543..23546 /locus_tag="Mup41a" CDS 23552..23986 /codon_start=1 /transl_table=11 /locus_tag="Mup41" /product="tail assembly chaperone" /label=Mup41 /note="in the structurally homologous position, the protein in bacteriophage P2 (and lambda) contains a programmed translational frameshift which is verified in PMID 15469818; contains InterPro domain IPR019289 for phage tail assembly chaperones" /db_xref="GeneID:2636287" /protein_id="NP_050645.1" /translation="MDEMNLGPEAQELHDSIVAEIQSGVLKLKDGLPFGTGDETEMQYD VTLRELTAGDMIDAQAAAEKLVMSKEGPVLVSSPSRMGLEMLRRQIASVGCIKGPLSMA LIRKLSVDDFQRLSLATEMYDMAVAASLTQERGRVAAVPE" CDS join(23552..23965,23964..24116) /codon_start=1 /transl_table=11 /locus_tag="Mup41a" /product="tail assembly chaperone frameshift product" /label=Mup41a /ribosomal_slippage /note="verified -2 translational frameshift in PMID 15469818" /db_xref="GeneID:2636287" /protein_id="NP_050645.1" /translation="MDEMNLGPEAQELHDSIVAEIQSGVLKLKDGLPFGTGDETEMQYD VTLRELTAGDMIDAQAAAEKLVMSKEGPVLVSSPSRMGLEMLRRQIASVGCIKGPLSMA LIRKLSVDDFQRLSLATEMYDMAVAASLTQERGREWLRCRNDIEKAATAIGVILKSGPE WALSLPLSRFFRHCQQAKTLSQYHR" gene 24120..26203 /locus_tag="Mup42" /label=Mup42 /db_xref="GeneID:2636275" RBS 24120..24123 /locus_tag="Mup42" CDS 24131..26203 /codon_start=1 /transl_table=11 /locus_tag="Mup42" /product="tape measure protein" /label=Mup42 /note="gp42; based on position and weak similarity with other tape measure proteins, as well as InterPro IPR013491 the Caudovirus N-terminal tape measure domain" /db_xref="GeneID:2636275" /protein_id="NP_050646.1" /translation="MTGKRLKASVIIDLNGNLSRRSRQYSNQINALSRSGQSSLRALRM EVVRVSGAIDRMGSLSTRTFRMLSAGALGIAGVGYTANKLFIGAAAQREQQIIAMNSLY HGDKVRAQAMMAWAKQNAKDTTWGLSGVLDEIRSSKGFGMTDEQTKQFITMLQDQGAMH GWDLPTAQGASLQLKQMFARQQITAADANLLTGYGINVYQALADATGTDVKKIRDLGTK GKLGMKSILTVFRTLSEQSKGAQASAMNSWDGMFAQMEANLLEFRIKVANSGPFEEIKN EMRRVLNWHDMADKSGELDALAENIGQKFLTTFRTVKISAQELWRWLKPGKDALAWVDQ NIVSLKKLAAVLVSVWLANKALRAGWAVAKPSWQVASYPFKTGRRMWRWMRNRKRGQAG LPVPDAMTSETLLQGIGIQRVFVINWPRGFGDYGSGGGRRVRSGGRMAPLLPRQPLLLS GPQPLALPAPRPVLALPPPGVPVTARPAPLPLPGKSGLLSRLAGSAAGQLVTGTVGKLA DAGRAVGGWFSGIGNKLAGSAIGRVVTKGAGALGWMGKGAGRALSRLGGPVMGALQLAP VLMDEQASTHEKAGAIGSTAGAWLGGAVGSLAGPLGTVAGATLGSVAGEYLGGFVTDLY QKWTATDKEPQEQKVNAEASLRVELGEGLRLTSSRVTEDGMGLNIYAGDNYITGW" misc_difference 24268 /locus_tag="Mup42" /replace="a" gene 26199..27695 /locus_tag="Mup43" /label=Mup43 /db_xref="GeneID:2636283" RBS 26199..26201 /locus_tag="Mup43" CDS 26208..27695 /codon_start=1 /transl_table=11 /locus_tag="Mup43" /product="DNA circularization protein" /label=Mup43 /note="N; previously published sequence has a frameshift near the N-terminus; also a baseplate hub protein see PMID 27555589; on injection and circularization see PMIDs 3023361, 11894948, 6306260" /db_xref="GeneID:2636283" /protein_id="NP_050647.1" /translation="MFEDALNAVNAVRDKTGGGRKTTGKGTFRNVPFLVIEEQKQAGGR RLVKREYPLRDTGGVNDLGKKLRSRTFSACILNSNAETARDEAGALMDALDAPGSGELV HPDFGTVDVMVDSWECRTKADELNYYAFTVTVYPSLQDTAPDAETDTSAAVPAQAVAVT GSLGDTLSSVWQTVKDGTAAATAVMEAVTGVIDDISDAVDNLGVTQTVSGLMGSLSAMK GSVTSLINQPAMLASSLMGALSGVSSLCDTRTAFSTWNRLAQRFERRHAATAGRQGTIT TSYNSPVAEKNIATLNYVMLAAAQTYRAEAASQALTAALDFSRRMDNAARAPVLDAPST TTGTASGASSTSATVTQGQLQLTAITPDGGFSQVSFSDSGTATPPVFESVSDIEKTTAM LGAALDSVILTASEQGFSTDSVQLTQLRLLVVADLEKRGLQLAGSESHHLPETLPAMVA LYRFTGNSRNWQRLARRNGISNPLFVPGGVSIEVINE" gene 27678..28827 /locus_tag="Mup44" /label=Mup44 /db_xref="GeneID:2636285" RBS 27678..27682 /locus_tag="Mup44" CDS 27688..28827 /codon_start=1 /transl_table=11 /locus_tag="Mup44" /product="baseplate hub protein" /label=Mup44 /note="Mup44 or P; see PMIDs 16125724 and 27555589" /db_xref="GeneID:2636285" /protein_id="NP_050648.1" /translation="MSNTVTLRADGRLFTGWTSVSVTRSIESVAGYFELGVNVPPGTDL SGLAPGKKFTLEIGGQIVCTGYIDSRRRQMTADSMKITVAGRDKTADLIDCAAVYSGGQ WKNRTLEQIARDLCAPYGVTVRWELSDKESSAAFPGFTLDHSETVYEALVRASRARGVL MTSNAAGELVFSRAASTATDELVLGENLLTLDFEEDFRDRFSEYTVKGYARANGAEGDD IDAKSIVSRKGTATDSDVTRYRPMIIIADSKITAKDAQARALREQRRRLAKSITFEAEI DGWTRKDGQLWMPNLLVTIDASKYAIKTTELLVSKVTLILNDQDGLKTRVSLAPREGFL VPVESDRKNRKGGDSNGGIDALVEDYYRRHPEKTPPWKE" gene 28800..29408 /locus_tag="Mup45" /label=Mup45 /db_xref="GeneID:2636280" RBS 28800..28803 /locus_tag="Mup45" CDS 28815..29408 /codon_start=1 /transl_table=11 /locus_tag="Mup45" /product="baseplate spike protein" /label=Mup45 /note="gp45; maybe P2 gene V homolog; see PMIDs 22922659 and 27555589" /db_xref="GeneID:2636280" /protein_id="NP_050649.1" /translation="MERVNDSALNRLLTPLMRRVRLMLARAVVNVINDGRKVQNLQVGL LDDEESDEVERLQNYGHFSVPLPGAEALIACVGAQRDQGIAVVVEDRRYRPTNLEPGDA GIYHHEGHRIRLTKDGRCIITCKTVEVYADESMTVDTPRTTFTGDVEIQKGLGVKGKSQ FDSNITAPDAIINGKSTDKHIHRGDSGGTTGPMQ" gene 29393..29842 /locus_tag="Mup46" /label=Mup46 /db_xref="GeneID:2636291" RBS 29393..29395 /locus_tag="Mup46" CDS 29405..29842 /codon_start=1 /transl_table=11 /locus_tag="Mup46" /product="baseplate wedge protein" /label=Mup46 /note="gp46; see PMID 27555589" /db_xref="GeneID:2636291" /protein_id="NP_050650.1" /translation="MTDLAIIWTNGRGDIAQDGIDMLTDDSLTTDVTISLFTDRRALDS DTLPDGSDDRRGWWGDSYRDRPIGSRLWLLSREKATPDTLERARGYAEEALEWLKTAGR VSAINVRAEQLHQGWLYLYIALTLPDGSVIPYEFKAAFNGV" gene 29834..30925 /locus_tag="Mup47" /label=Mup47 /db_xref="GeneID:2636250" RBS 29834..29837 /locus_tag="Mup47" CDS 29843..30925 /codon_start=1 /transl_table=11 /locus_tag="Mup47" /product="baseplate wedge protein" /label=Mup47 /note="gp47; see PMID 27555589" /db_xref="GeneID:2636250" /protein_id="NP_050651.1" /translation="MAYSPPTLSSLIARTEQNIEQRLPGSWPQAREKTLSAIAYAQAGL AAGCHEHISWVGRQIIPSTADEDELLEHCRFWGVRRKQATAASGPLTVTTSAATTIPAG TRWQRADGVVYSLADTIVIDRAGTTEITVTALAAGEAGNTGENTLLTLITPVACVVSDA ITVKGFSGGADIESAAELLSRLEYRVQYPPFGGNQFDYVRWAREVSGVTRAWCFPTWKG GGTVGVTFVMDNRSNIFPQPADVERVADYIAGHTDPITGLIVGQPDGVNVTVFAPKAKP VNPRIYISPKTAELKQAITNAINTMFFNEVMPGGALAPSRIIRAVAGVTGLDDFEVRFP TEIQRSENTELLTAGTIEWL" gene 30904..31458 /locus_tag="Mup48" /label=Mup48 /db_xref="GeneID:2636277" RBS 30904..30907 /locus_tag="Mup48" CDS 30916..31458 /codon_start=1 /transl_table=11 /locus_tag="Mup48" /product="baseplate wedge protein" /label=Mup48 /note="gp48; see PMID 27555589" /db_xref="GeneID:2636277" /protein_id="NP_050652.1" /translation="MAVTPWQTAFLQLLPSGLAWNKSPDSKLSALAQAISDVIATAADD ARQMLRERFPSTSRWYLGEWESFLGLPDCTSENGTLSERQRAAANKMRMTGNLSRRFYE WLAAQYGFTVRLTDSTEGQWVTQVNIYGIKNYRNATVLDNVLTPLRVYESGALECLLEK YKPAHQIYKFVYHDGDN" gene 31447..32972 /locus_tag="Mup49" /label=Mup49 /db_xref="GeneID:2636293" RBS 31447..31450 /locus_tag="Mup49" CDS 31458..32972 /codon_start=1 /transl_table=11 /locus_tag="Mup49" /product="tail fiber S" /label=Mup49 /note="S; responsible for host range see PMID 6250048 and 31209305 for structure" /db_xref="GeneID:2636293" /protein_id="NP_050653.1" /translation="MFYIDNDSGVTVMPPVSAQRSAIVRWFSEGDGNNVITWPGMDWFN IVQAELLNTLEEAGIQPDKTKLNQLALSIKAIMSNNALLIKNNLSEIKTAGASAQRTAR ENLDIYDASLNKKGLVQLTSATDSPSETLAATAKAVKIAMDNANARLAKDRNGADIPNK PLFIQNLGLQETVNRARNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSD TDSYMWFETGDNGNEYFKWRSKQSTTTKDLMNLKWDALSVLVNAIVNGEVISKSANGLR IAYGNYGFFIRNDGSNTYFMLTNSGDNMGTYNGLRPLWINNATGAVSMGRGLNVSGDTL SDRFAINSSNGMWIQMRDNNAIFGKNIVNTDSAQALLRQNHADRKFMIGGLGNKQFGIY MINNSRTANGTDGQAYMDNNGNWLCGAQVIPGNYANFDSRYVRDVRLGTQSLTGGLSRD YKAPSGHVITGFHTNGDWEMQGGDDKVYIRPVQKNINGTWYNVASA" misc_feature 31458..31966 /locus_tag="Mup49" /note="encodes amino terminal end of tail fiber protein(S')in G(-) phage" misc_feature 31966 /locus_tag="Mup49" /label=G-segment boundary /note="G-segment boundary" gene 32963..33502 /locus_tag="Mup50" /label=Mup50 /db_xref="GeneID:2636270" RBS 32963..32965 /locus_tag="Mup50" CDS 32975..33502 /codon_start=1 /transl_table=11 /locus_tag="Mup50" /product="tail fiber assembly chaperone" /label=Mup50 /note="U; see PMID 31209305 for structure" /db_xref="GeneID:2636270" /protein_id="NP_050654.1" /translation="MMHLKNIKSENPKTKEQYQLTKNFDVIWLWSEDGKNWYEEVNNFQ DDTIKIVYDENNIIVAITKDASTLNPEGFSVVEIPDITANRRADDSGKWMFKDGAVVKR IYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERARLESWERYSVLVSRV DTANPEWPQKPE" gene complement(33531..34079) /locus_tag="Mup51" /label=Mup51 /db_xref="GeneID:2636259" CDS complement(33531..34064) /codon_start=1 /transl_table=11 /locus_tag="Mup51" /product="tail fiber assembly protein" /label=Mup51 /note="Predicted by GeneMark" /db_xref="GeneID:2636259" /protein_id="NP_602302.1" /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYTEDGKNWYEEQKNFQ PDTLKMVYDHNGVIICIEKDVSAINPEGANVVEVPDITANRRADISGKWMFKDGVVIKR TYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKVEST DTSSLPVTFPEQPE" misc_feature complement(33531..34064) /locus_tag="Mup51" /note="encodes tail fiber assembly protein (U') inG(-)phage" gene complement(34067..35053) /locus_tag="Mup52" /label=Mup52 /db_xref="GeneID:2636274" CDS complement(34067..35053) /codon_start=1 /transl_table=11 /locus_tag="Mup52" /product="tail fiber fragment" /label=Mup52 /note="S'; N-terminus encoded by Mup49 when in G(-) orientation; see PMID 6250048" /db_xref="GeneID:2636274" /protein_id="NP_602303.1" /translation="MPKSTIIQNLGLQETVNQASGALQQNQNGADIPGKDTFTKNIGAC RAYSAWLNIGGDSQVWTTAQFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNIC LAGAVVEVIGTRGAMTIRVTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQP AFALGQTGSTVGNDKAVGWNWNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNG GIFYRSARDGYGFEADWSEIYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLSSVQTW NGPGWSDRSGYVVTGSVNGNRDELIDTTQARPIQYCINGTWYNAGSI" misc_feature complement(34067..35024) /locus_tag="Mup52" /note="encodes carboxyl terminal end of tail fiberprotein(S') in G(-) phage" RBS complement(34076..34079) /locus_tag="Mup51" misc_feature 35025 /label=G-segment boundary /note="G-segment boundary" gene 35080..35673 /locus_tag="Mup53" /label=Mup53 /db_xref="GeneID:2636258" RBS 35080..35084 /locus_tag="Mup53" CDS 35092..35673 /codon_start=1 /transl_table=11 /locus_tag="Mup53" /product="serine recombinase Gin" /label=Mup53 /note="G-segment invertase" /db_xref="GeneID:2636258" /protein_id="NP_050655.1" /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPGL KRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGRFF FHVMGALAEMERELIIERTMAGLAAARNKGRIGGRPPKLTKAEWEQAGRLLAQGIPRKQ VALIYDVALSTLYKKHPAKRAHIENDDRIN" gene 35758..35956 /locus_tag="Mup54" /label=Mup54 /db_xref="GeneID:2636254" RBS 35758..35762 /locus_tag="Mup54" CDS 35768..35956 /codon_start=1 /transl_table=11 /locus_tag="Mup54" /product="translational activator Com" /label=Mup54 /note="gp54; translational regulator of Mom; see PMID 2934296" /db_xref="GeneID:2636254" /protein_id="NP_050656.1" /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTEK HCGKREKITHSDETVRY" gene 35877..36602 /locus_tag="Mup55" /label=Mup55 /db_xref="GeneID:2636302" CDS 35877..36602 /codon_start=1 /transl_table=11 /locus_tag="Mup55" /product="adenine modification enzyme Mom" /label=Mup55 /note="DNA modification, adenine acetyltransferase just prior to packaging; see PMID 1258376" /db_xref="GeneID:2636302" /protein_id="NP_050657.1" /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETI SCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNR GYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGRAGVVYQASN FDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGVYLRANKERAVVHKFNQYRYIRFLN KRARKRLNTKLFKVQPYPK" RBS 35896..35899 /locus_tag="Mup55" BASE COUNT 9110 a 8932 c 10181 g 8494 t ORIGIN 1 tgtattgatt cacttgaagt acgaaaaaaa ccgggaggac attggattat tcgggatctg 61 atgggattag atttggtggg gcttgcaagc ctgtagtgca aattttagtc gttaatcaat 121 gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg aaaatacgaa aaacaaaaac 181 actgcaaatc atttcaataa cagcttcaaa aaacgttcaa aaccgataac aaccaagctg 241 tcaccaaatg actcatatca caaatcagct tatgccgttt aggtatgtta catgtgtgat 301 tatgtgaggt gaagtatgtt ttagctggtt catggttgtt atacggcttt ttttacctcc 361 tgtggttcct gtgaaggtac tacaacactt tcctgttcat gaatcccata ctttgacaaa 421 atctctttgc gtttttcttc aggtaatgca tctagcatca tcaacgtctg aatactttgc 481 tgtgaaaatc ctataaagct gtaaagtttc tgttcattaa atacaagagg cattaacgcc 541 aacaaccccc ctttacttaa aagtttcagt gccttgcgtg cttcatctgg ttccagctct 601 tcaatcatat tgattaaggt tgtggttaat ttgtttatca gttccgaaga atcctgtttc 661 tcattggctt gagcaccagt atccggtgtg gataacccca agtgcgcaat aacttgctct 721 cgttctttgg tgggcatcga catcacatcg tattcaacag ctttcccccc cttgacacct 781 tccttttttt gcttcgtcca gccttgaaca tttgctcgat agtgaacacc agcaacagat 841 ccaggcatac catcagcagc cataatttct tgcggcgaac accaaattga cttttcagta 901 ttattctttt ctataaagtt acttttcaaa atttaaactc cttatttatc aacgcgttaa 961 tcagtaatca aaggaattta ccaaaaagca gctttacaaa aagcttttca gtaattatct 1021 ttttagtaag ctagctaagt ttttacactt agttaaattg ctaactttat agattacaaa 1081 acttaggagg gtttttaaat gtgttccaac gaaaaggccc gtgattggca tcgtgcggat 1141 gtgattgcgg gacttaagaa aagaaagctc tctttatcag ctctttcccg gcagtttggt 1201 tatgcgccaa ctacattagc taatgcgcta gaacgacact ggccaaaggg tgagcagatt 1261 attgctaacg ccttagaaac taaaccggaa gtaatctggc ctagccgata tcaagcaggt 1321 gaataacatg gaactttggg tatcaccgaa agagtgtgcg aatcttcctg gtttgccgaa 1381 aacatcggct ggtgtgattt atgttgctaa aaagcaagga tggcaaaacc gcactagagc 1441 aggtgtcaaa ggtggtaaag caattgaata caatgcgaac tctttacctg ttgaagcgaa 1501 agcggcgtta ttgctgagac aaggagagat tgaaacaagc ctggggtatt ttgaaatcgc 1561 ccgccccacg ctggaagccc atgattatga tcgtgaggca ctgtggagca aatgggataa 1621 cgccagcgat tcccagcgca gacttgctga aaaatggttg cctgcggttc aggctgcaga 1681 cgaaatgctg aaccagggga tttcaacgaa aacggctttt gcgaccgttg cagggcatta 1741 ccaggtcagc gcatccactt tgcgggacaa gtattaccag gtacagaagt ttgcgaagcc 1801 tgactgggcg gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg ttcacaaaag 1861 tgaatttgac gaggatgcct ggcagtttct gattgcagat tatctgcgac cggaaaaacc 1921 cgctttccgc aaatgttatg agcgtctgga actggcagcc cgcgagcatg gctggagtat 1981 tccctcccgt gccacggcct ttcgccggat tcagcaactg gacgaggcaa tggttgttgc 2041 ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg gcacagcagc gaactgtgga 2101 acacctggac gccatgcagt ggatcaacgg cgacggttat ctgcataacg tctttgtacg 2161 ctggtttaac ggtgatgtga tccgtccgaa aacatggttc tggcaggatg tgaaaacccg 2221 aaaaattctg ggctggcgct gcgatgtgag cgagaacatt gattcaattc gcctctcgtt 2281 catggatgtt gtgactcgct acggtatccc ggaggatttt cacatcacca ttgataacac 2341 ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc aatcgctacc gctttaaggt 2401 aaaagaggac gatccaaaag gactgttttt actgatgggg gcgaaaatgc actggacaag 2461 cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt tcggtgttgg 2521 tgggcttgag gaatacgttg ataagcatcc ggcactggct ggcgcatata cggggccaaa 2581 tccgcaggca aaacctgata actatggcga ccgcgctgtt gatgcagagc tgtttctgaa 2641 aacccttgcc gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa cagaaatgtg 2701 cgggggcaaa ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa cgattgtgcg 2761 taagccaacc gaagaacaaa aacggatgct gttactgcct gccgaggcgg tgaacgtttc 2821 acgcaaaggc gagtttacgc ttaaagttgg cggctccctt aaaggcgcga aaaacgttta 2881 ttacaacatg gcattaatga atgccggcgt gaaaaaagtt gtggtcaggt ttgatccgca 2941 gcagctacac agcacggttt attgctacac cctggacggt cggtttatct gtgaagcgga 3001 atgtctggca cctgttgcat ttaatgatgc tgcggcaggc cgtgaatatc gccgccgcca 3061 gaaacaactg aaatctgcga cgaaagcagc cattaaggcg cagaaacaaa tggacgcgct 3121 ggaagttgct gaactgctgc cgcagatagc cgaaccagca gcaccagaat cacgaattgt 3181 tggtattttc cggccttccg gtaatacgga acgggtgaag aatcaggagc gtgatgatga 3241 atacgaaact gagcgtgatg aatatctgaa tcattcgctg gatattctgg aacagaacag 3301 acgtaaaaaa gccatttaat taacgtttaa acaaaattta attacgaggt tattcagatg 3361 aatatttccg atattcgcgc aggactgcgc acgcttgtag aaaatgaaga aaccaccttt 3421 aaacaaattg ctcttgagag cgggctttct accggaacta tcagtagttt tatcaatgat 3481 aagtacaacg gggataacga gcgtgtttca caaatgctgc aacgctggct ggaaaaatat 3541 catgcagtgg cagaactacc tgaaccgccc cgctttgtgg aaacgcagac ggtaaaacaa 3601 atctggacaa gtatgcgttt tgccagcctg actgaaagta ttgctgttgt atgtggcaat 3661 cctggtgtgg gcaaaaccga agcggcccgt gaatatcgcc gcaccaataa caatgtctgg 3721 atgatcacca ttacgccatc ctgtgccagc gttctggaat gtcttactga actggcgttt 3781 gagctgggaa tgaatgacgc accacgccgt aaagggccgc tctcccgcgc cctgcgacgt 3841 cgccttgaag gtacacaggg gctggttatc atcgacgaag ctgatcatct tggtgccgaa 3901 gttctggaag aactccgcct gttacaggaa tcaacccgta ttggccttgt gctgatggga 3961 aatcaccggg tttattcaaa tatgacgggg ggtaacagaa cggttgaatt tgcccgtctg 4021 ttttcccgta ttgcaaagcg cactgcaatt aataaaacca aaaaagccga tgtaaaagct 4081 attgcggatg cctggcagat taacggcgaa aaagaactgg agttattaca gcagattgcg 4141 cagaaaccag gtgcgcttcg cattctgaat cattcacttc gccttgcagc catgacggct 4201 cacggtaaag gtgagcgtgt taacgaagat tatctgcgtc aggctttccg tgaattagac 4261 ctggacgttg atatttcaac gctgctgcgt aattaagaag gagaagaaat tatgatggcc 4321 cgaaatataa aaatggcaac ggatgcgcag aactggttac aggcgcgcgg gagtcatgta 4381 aatgaatcat atctcggcgt ggcgcgtccg attcttgaaa tcacttaccc accggtggaa 4441 ctggtaaaaa acgcggtcag aattatggag cataagtccg gggtggcccg ttctgtatgg 4501 acggcccgtc ttaatggttg ccagattatc tggagataac gatgtgtatt aaagctgaaa 4561 aatatattga atgggttaaa cactgtcagt gccacggggt gccgctgacg acatataaat 4621 gccccggatg tggtgaacag attatgacgc aatgctcacc cgaaaaggaa attcgggatt 4681 ccctgacatg ttgtccgtgg tgcagcgcag ttttctttaa acaggtaaaa ggcgcgaagg 4741 taaaagccag cgcggttatt cagaatcaat aaggtgaaac aaaatggcaa aagtaattat 4801 tgaaattaaa aatacagtat ctggaattaa aggacggaat ttgcgcacca gcattgcggt 4861 ggacggtagt gcggaactgg acggtgatga gggtacactt gctggcatgg tggcgttact 4921 ggtgcttaat aaaagccaga aaataattaa tgagtcagcc catgaagcta ttgagatttt 4981 gaaaaacgat ggtgtcatca ccagtggtcg tgtcactgaa atggctgttg aaaaaacatg 5041 tcactgaaag gaacgctgac aatgaatgtc aaaatccgaa atgaaattca ggcattaatc 5101 cgaattcagg aacgcaataa caacggtggt gaattacgcg agtttatttg tgcgcgtgaa 5161 gttgatggct atggtgaaaa aacttacctg attactttcg accattacag catctgtgcg 5221 cgttattgcg gcgaaagcat atcccgcgcc attgcgtctg gcgatgcgtt taacgtggat 5281 ttatgggagt acgtcatgga ccgggaatac atctgcgcat cagaccctga agcccgtgaa 5341 atgtggcagc gtatctggcg cgattaccgg ttaatggcaa aaggctgggc gcgctgctgt 5401 tattcctcgc ttgccctgaa agcggttcag ttatcgctgc ggcatattcc ggcatcactg 5461 cgcgagcctc tgctgtactg aatggtgact gacatgaagt gtaatcgtaa gcgctggtca 5521 cgcgaagacc gggaatttat cgaagccaac gtcgggaaaa tgaccgttga agaaatggcg 5581 gaaaaactga aagtcgccac aaccgccctc cgggcacatg ccagaaggca cggaatatca 5641 ttgtgtgtat accgaatcag tgaacacgac aaatatttat gtcgtgaact ttataaagaa 5701 ggactggata ttcatgttat tgcccgaaag atggaattaa gcaatcgagc tgtatccagc 5761 attgtataca gcggatatta attaacagga gctttaattt atggctaaac cagcaaaacg 5821 tatcaagagt gccgcagcgg cttatgtgcc acaaaaccgc gatgcggtga ttaccgatat 5881 taaacgcatc ggggatttac agcgcgaagc atcacgtctg gaaacggaaa tgaatgatgc 5941 catcgcggaa attacggaga aatttgcggc ccggattgca ccgattaaaa ccgatattga 6001 aaccctttca aaaggcgttc agggatggtg tgaagcgaac cgcgacgaac tgacgaacgg 6061 cggcaaagtg aagacggcga atcttgtcac cggtgatgta tcgtggcggg tccgtccacc 6121 atcagtaagt attcgtggta tggatgcagt gatggaaacg ctggagcgtc ttggcctgca 6181 acgctttatt cgcacgaagc aggaaatcaa caaggaagcg attttactgg aaccgaaagc 6241 ggtcgcaggc gttgccggaa ttacagttaa atcaggcatt gaggattttt ctattattcc 6301 atttgaacag gaagccggta tttaatacca ccattaatat ttaattaatt cacattcttt 6361 taattatggc gcgatacgtc aggagattgc tcgcgcctga aacagattac tgaggaataa 6421 aacatggttg atgcaaaaat tctgaatggt gtcagcacat tattacgggc ttacggacgc 6481 ctgacctgcg gagttctggc tgaaaaaatg aatatgctgc cctcgtcaat ggtgtatttc 6541 ctgcgtgatg cggttgatgc cggagtgctc accgaatgca acggatttta tgacgttccg 6601 cgcccgcgtc cgacgccgcc tgtaagacga aacgcaactg agcagcctgc tgttgatgat 6661 gcggtgtggt gcaactggcg tcgctcatta ccctgggtgg aaggtaatac catccccgcg 6721 ctggcaaaag agtttgcgac gggcgtgctg acctgtgagt cagtccacat tgttgctgag 6781 gtggataaca gaatgtgcga acagggaatg ccccgttttg tgatggctta tatcgatatc 6841 cggctggggc gttttatttg cagttcaagc gtctggaata tcaccgacca tgtactgcgt 6901 tatctcattc ttgattgttc tccggctccc gcagcggtgc aggaggtggc gtgatgtttt 6961 ttaaaacgtc aaatcccgca gcactgctgg cgtgggacca gtttatggcg gactgtctga 7021 aactacgcga agaagcccgc catcttgata aggttctggg ttgtggttgc cggtcagtat 7081 tcagcacaag tattggtggg cgttattttc atggtgtgaa ctttccgggg aatgagcgcc 7141 ctttctcgcg ggagctgtgg accgtacaac ggcccgcatc aggtaacagt tgcaggccca 7201 gaacttcgcg gatacctgct catctcaggg agcaggcaag agagctggcg aaaatatggc 7261 aggagaatat tccggtcact tacgcccgca cagacgctct tttacccgca ctgggactgg 7321 atttcagcgc aacaatattc gggccgctcc agtggttccg tgttggtgat gtgatttacg 7381 tcatgacggg gatgacaccc gcgcaaggcc gcatgactga aatcctttct gacgaattta 7441 tccgggcgca aaaacaggcg gaggtaaaca atggaaaaca ataaaacatc gtattcgtgg 7501 ctggggaaat tcaccacggt gaaacaggaa tgcccgacct gcggtaatga atcccctgaa 7561 tatctgaaag agtgccctca ttgtggcggg ctgaaatgca accactgcga tatgggcgat 7621 gacacagcgt gcatgaattg tgaaggtgaa taatatgaat aatgaaacta aatttactcc 7681 ccttaatatt gataacgtca tggctgaaaa agggatgctg gaacgtgttc gcgctatcgt 7741 tgaatatggt attaagcata atctcaccgc cagagaagtc cgtgacatta tcaaccgcga 7801 aatgaatcgt ctggaaacag tagtggcact gcaaaatgaa acagcccgcg aggagtacat 7861 ccgccgcagg ctggggctat cagaccagga tattgttact gacgctcatg tgtttgaggc 7921 ttttgagatc cgtcagcatc tgggtctaac gaactgataa tgtcttccat gatctttgac 7981 agttgcagtt tttcgcggcg gcttgttctc attgcaggcg catcttttaa aatctctgct 8041 gaagagctga aaatggcttt gccctcttct gcggtgatga tcgccttctt caccagaagg 8101 cgcaccagtg ctgatgcaat cgcataacct gcaacatcat aaggttgtac attattttcc 8161 ataaaatcct ctctgattga tgtgtaaatt caggcggtgt tgcagcaccg ccccctttcc 8221 ggaggtacga tcatgagtcg cacatccctg attaaattaa tccatgtcgc ccgtcgggag 8281 ctacaactcg acgacgacac ttaccgcgct tttctgatgc agaaaacggg caaaatcagt 8341 tgccgcgagc tgacggtcac gcaactggaa caggtgctcg gtgccatgaa agaacgcggc 8401 tttaagaagc agaataaata cccccgtcgc cgctttaagg ggcatgtcac gccccgtgag 8461 aaggtgtata aaatctggca gcagatggca gaagacggtt ttattaccga tggcggtgat 8521 gtggcgctgg ataaatatgt tcagcgcctg accgcgaaac gcaacggcgg acagggcgtt 8581 tccacactgg cgtggtgtca tggcgacacg ctgctgacag tcctcgaaac actcaagcag 8641 tggcacatcc gctgtattcg tgaagccttt tcccgtcacg ggcttccatt gccggtcagt 8701 ccgtccggtc gtgaactgcg cggctatgat gccatgacgg cggcttatgc ccatgccaga 8761 aagacacgga ggatggcaca atgacggaag atttatttgg tgatttgcag gatgacacca 8821 tcctggcaca tcttgacaat cccgccgagg atacgtcacg ctttccggca ctgctggcgg 8881 agctgaacga tttactgcgc ggggaattgt cccggctggg tgttgatcct gcccattcac 8941 tggagattgt tgtggctatc tgtaagcatc tgggcggcgg tcaggtctac attccacgcg 9001 gtcaggctct ggactcgctg attcgggatt tgcgtatctg gaatgatttt aacggtcgca 9061 acgtcagcga actgaccacc cgttatggtg tcactttcaa tacggtgtac aaagccattc 9121 gccggatgcg acggctgaaa taccgccagt accagccctc acttctgtaa acagtaaagc 9181 cggttaatcc ggcttttttt acgtcctcaa tatcctgtga tgaataaccg taccggggaa 9241 aatcatcatg ggtaaaggct ggaatgcatc ctttcatctt ggcagacgtg agcggctgcg 9301 tcaggaggtt ttgcaccgtg tcgccggtgg gccacgtcct gcgccccgcg actataccgg 9361 tcatgatggc acccacggca gttattacat gaaaggctgg cagtcagttg atatgccgga 9421 aattcttcat cattgcctgc tttacaggga gaaacattat gtctgagcgt tctgctcgtc 9481 agtggcctga ttttttatcg gtggtgttac tggcgctgtt attgtggata agtctgtttt 9541 gtggctggcg cgcgctgatg ttttgctgtg cctcggtttt cagtgttgct ttatgtgttg 9601 ccgctgattg tctggatgcg ctgataatga gctgccgggt gcctgaacat tttgcccggt 9661 tcgtctggcc tctgacctgg ctggggagtc tgtccgggct gggattagcc gtgatggcaa 9721 cgtctcaact gaaaacgggt cctgagcatg tcatctgggc gctggccgga ttgctgacgt 9781 tctggctctc atttcgtttt cgcgctcgtc tgttcgggta aaggggcacc atgtacagaa 9841 aattcagtga tgaatgtttc gggccgtcca cgctgattaa tgcgataaaa gtgattgccc 9901 ttgtggttct gataaccatc agtgccgtgg tgtatctttc tgtctgttaa ccgggggaaa 9961 atatgcagca tgatttattt gaacacgatc cggcaattcg ccagttaatc ggacatattg 10021 ataacatccc ggccccggaa ctggaatcac gctggccccg ttcggtggtt gatctgattg 10081 atgttctgga aaacgaactg aaacgccaga acgtcagcaa tccccgtgaa cttgcccgta 10141 aacaggccgt tgccctgtcc tgttttctgg gtggacggca gttttacatc ccctgcggcg 10201 atacgatcct gaccgcgtta cgcgacgatt tgctttactg ccagtttaat ggtcgtaaca 10261 tggaagaact gcgtcgccag taccgcctgt cccagccgca gatctaccag attattgccc 10321 gccagagaaa gctgcataca cgacgtcatc agcctgatct gttctcacct gaaaccccaa 10381 aataaccggc atcctgccgg ttatttcctg tcaccataat cccgcacctg ccacctgatt 10441 ttttagactg ccatcagaga attttttcag ggaagcatga tggcggggat accaaaaaaa 10501 ctgaaagccg cactgctggc tgtaacgatt gccggtggcg gagtgggtgg ttatcaggaa 10561 atgacccgtc agtctctgat ccacctggaa aatatcgctt atatgcctta ccgcgatatt 10621 gccggtgtgc ttacggtctg tgtgggacat accgggccgg atatcgaaat gcgccgttac 10681 agccatgcgg agtgtatggc tctcctcgac agtgatttaa aaccggttta tgcggcaatc 10741 gatcgtcttg tcagggtgcc gctgacgcca tatcagaaaa cggcgctggc aacgtttatt 10801 ttcaacaccg gtgttaccgc attcagtaaa tcaacgctgc tgaaaaaact caatgccggt 10861 gattacgccg gagcgcgtga ccagatggcc cgctgggtgt ttgccgcagg ccataaatgg 10921 aaaggactga tgaaccgtcg cgaagtggaa atggcaatct ggaatatcag gggggcggat 10981 gaccttcgcc agtaaaagtt tactactggc agccgtgttt accgccgttc tgtccggggg 11041 gttgtggcac aggctggaca gcacccgcca cgataaccag acgctgcgcc gtgaattaca 11101 gacggagcaa caggcccgcc acactgctga atggttgtta cacggtcagg aacagacgat 11161 gcaggtattt tccgctatcc gtgccgcaaa tcgtgccgca cggctggcag atgagacaga 11221 acatcatgat gcaaaagaaa aaattacaac cgccattact ggtgacaatt gcagcactcg 11281 tcctgtgcct gccgttgctg ctgacaggtt gcgggaactc gaaaaacgca cccgtgccat 11341 cggtggtgat cctgcccgaa attgatacag aactgacgga agcaacgccc gttccgccca 11401 tgccacagcc cctgacgtgg ggagcatccc ttctctggaa tgctgacctg ttgatggcgc 11461 tcgggcagtg caaccgcgac aaggcatcag tccgggaaca ggagatccga aggaaagaaa 11521 tttatgaacg aagaccagaa ccgggcggtg gcgctgctgc tcgctgaact ctggcagggg 11581 gatacccgtg atattccccg cccggcggcg tatgacccgc ctgtattgtg tgccggttgc 11641 ggtcgtgagc tgcggcctga tgtgctgaga cagcagccga tggcgaacta ctgccactgg 11701 tgccgtggag cagaataatg gatttgattt cagttttagc gttatggcct tacctgttgc 11761 ctgttgtggc cggtggggcc gtctgggcga tgcgtcgtag ttttgccagc accgagcgtg 11821 tggagcgtct tgaaaaccgg atgaccgaaa tggaaacccg ctacgccagc atccctggca 11881 cagaagatgt acacgaaatg cgcctgcgaa ttgcagagct gtccggcgat atccgggtgc 11941 tgtcccagcg ggtgcagtca ttttcccatc aactggagct gctgcttgaa aacgcagtaa 12001 accggagtaa ttcatgatta acgatattct gaccgaagac cgtcgtctgg tcatcctgcg 12061 ttccctgatg gactgcaata acgaagcaaa cgaatccatc cttcaggact gtctggatgc 12121 atacggacac aacgtttccc gcgatgtggt tcgcggccag attgactggc tggcagagca 12181 gcaactggtc accgtggaaa acctgcgcgg cttttatgtc gtaacgctga cctcccgagg 12241 tcaggatgtg gcagaaggtc gtgcccgcgt tgccggtgtc aagcgtcctc gtccccgtgc 12301 atgacaggag gcgtgatgga caggaaaaca cggggccggg cttcaaaagt ggatttactg 12361 ccggaaaacg tacgtaaaac gctgcatgaa atgctgcgtg ataaagcgat cccacaggcc 12421 cgcattctgg aagaaattaa cgcactgatt gaagacgcag gcctgccgga tgagatgaaa 12481 ctctcccgtt ccggtctgaa tcgttacgcg acgaacgtcg agcaggtagg ccataacctg 12541 cgacagatgc gtgaaatgac gtcagcactg accgcagaac ttggcgacaa gccaatggga 12601 gaaaccacaa agctgattct ggaaatggcc cgcagccagc tttttaaggc catgatgcgg 12661 caaatcgaaa acccggaatc tgacgtggat attgacctgc tgaaaaatgc catgctggcg 12721 gcacagcgtc tggaatccac ggcaatgtcc agccaccggc gcgagaaaga aatccgccag 12781 gcattcgcag aagaagcggc aaacgccgtc agtgaagaac tgcgcgggca ggatggcatc 12841 agcgaagagc tggagcagcg tatccgtgat gtactgctgg gtaaggcatg agagggttat 12901 gaacaccaga gaaaacaacc ttaaagccct tcatgcgccc cgcaaaatca acctgcgtga 12961 ggaagccgga ctgctcggcg tggatatcgt gaccgatatc ggggaagcac agccccgcaa 13021 tgaaccggtt tttctgggct atcagcgccg ctggtttgag gatgaaagcc agatttgtat 13081 tgctgaaaaa tcccgtcgta ctggcctgac gtgggccgaa gcaggccgca atgtcatgac 13141 ggcagcgaag ccgaagcgtc gcggtggtcg caatgtcttt tatgtgggtt cccgtcagga 13201 aatggcgctg gaatacatcg ccgcctgtgc cctgtttgcc cgcgccttta accagctggc 13261 aaaggctgat gtctgggagc agaccttctg ggacagcgat aaaaaagaag aaatcctcac 13321 ttatatgatc cgctttccga acagcggatt taagatacag gcgctgtcaa gccgcccgtc 13381 taacctgcgt ggtttgcagg gggacgtggt gattgatgag gcggcattcc atgaagccct 13441 ggacgaactc ctgaaagcgg cctttgcact aaacatgtgg ggtgccagcg tgcgcatcat 13501 atcgacacat aacggcgtcg ataatttgtt taatcagtac attcaggatg cgcgcgaggg 13561 gcgcaaggat tacagcgttc accgcatcac gctggatgat gccatcgctg acgggttata 13621 ccgacgtatc tgttacgtca cgaatcagcc gtggtcgccg gaagcggaaa aagcctggcg 13681 tgatggcctg taccggaatg cgccgaataa agagagtgcc gacgaggaat acggctgcat 13741 tcctaaaaaa tccgggggcg cttatctgtc acgcgtgctg attgaagccg ccatgacgcc 13801 agcgcgggat atcccggttc tgcgttttga agccccggac gattttgaat cgctgacacc 13861 acaaatgcgc cacggcatcg ttcaggactg gtgtgaacag gagctgctcc cgttgctcga 13921 tgcgctgtcg ccgctcaata aacacgtact gggcgaagac tttgcacgcc gtggcgacct 13981 gacggtgttt gtgccgcttg ccatcacgcc tgacttacgc aaacgcgaat gtttccgcgt 14041 ggagctgcgt aatgtgacct atgaccagca gcgacaaatc cttttattca ttctgtcccg 14101 tttgccccgt tttaccggcg ctgcatttga tgccaccggt aacggcggtt atctggcaga 14161 ggccgcccgt ctgatttatg gcccggagat gatcgactgc atcagtctga cccccgcctg 14221 gtatcaggag tggatgccaa aactgaaagg cgagtttgaa gcgcagaaca tcacgattgc 14281 caggcatcag accacacttg atgacctgct tcatatcaag gtggataagg ggatcccgca 14341 gattgacaag gggcgcacaa aagatgaagg cggtaaaggc cgacgacatg gcgactttgc 14401 cgtggcgctc tgtatggctg tcagggcgtc ttatatgaat ggttttgtta tcgacgagga 14461 cagcatacag gcgctgccac cacgccaccg tggtgatgat gttgataacg atgattttga 14521 tgattatcac cagtttgaac gagggggctg gtaatgggcc gtattctgga tatttccggg 14581 cagccgtttg attttgacga tgaaatgcag agccgcagcg atgagctggc aatggtcatg 14641 aagcgcacgc aggagcatcc ttccagtggt gtgacgccga accgggccgc gcagatgctg 14701 cgtgatgccg aacgcggtga tctgacagca caggctgacc tggcctttga tatggaagaa 14761 aaggacaccc accttttttc cgaactgagc aaacgccgcc ttgccattca ggcgctggaa 14821 tggcgcatcg ctccggcacg tgatgccagc gcacaggaga aaaaagacgc cgacatgctg 14881 aatgaatatc tgcatgatgc ggcgtggttt gaagatgccc tgtttgatgc cggtgatgcc 14941 attctcaagg gctattccat gcaggagatt gaatggggct ggctggggaa aatgcgcgtg 15001 ccggtggcgc tgcatcatcg cgatccggcg ctgttctgcg cgaaccccga caatctgaac 15061 gaattacgcc tgcgtgatgc gtcttatcac gggctggagc tgcaaccgtt cggctggttt 15121 atgcaccggg ccaaatcccg caccgggtat gtcggcacca atggccttgt gcgcacgctt 15181 atctggcctt ttatttttaa aaactattcc gtgcgtgatt ttgcagagtt tctggaaatt 15241 tacggactcc ccatgcgcgt cggtaaatat ccgaccggct caacgaaccg cgagaaagcc 15301 acgctgatgc aggccgtgat ggatatcggg cgacgtgccg gtggcattat tccgatgggg 15361 atgacgctgg attttcagag cgccgcagat ggtcagtcag acccttttat ggcgatgatt 15421 ggctgggcag aaaaagcgat ttcaaaagcc attctgggcg gcaccctgac cacagaggct 15481 ggcgataaag gtgcgcggtc gctgggtgaa gtgcatgatg aggttcgccg ggaaatccgt 15541 aacgcggatg tgggccagct tgcccgcagt attaaccgtg acctgattta tcccctgctg 15601 gcactgaaca gtgacagcac cattgatatt aaccgcctgc cgggcattgt atttgatacc 15661 agtgaagcgg gtgatattac ggcactgtct gacgccattc cgaaactggc ggcgggaatg 15721 cgtattccgg tctcctggat acaggaaaaa cttcatattc cacagccggt cggtgatgaa 15781 gccgtgttta ctatacaacc ggttgtcccg gataacggtt cgcagaaaga ggccgcatta 15841 tccgctgaag atatcccgca ggaggatgat atcgaccgga tgggcgtctc accggaagac 15901 tggcagcgtt cggttgaccc cctgctgaaa cccgtcattt tttctgtgct gaaagatggc 15961 ccggaagccg ccatgaacaa agccgcttca ttatatccgc agatggatga tgcagaactt 16021 atcgacatgc tgacccgtgc catttttgtt gccgatatat gggggcgtct ggatgccgca 16081 gcagaccatt gatctggcgt atgccgcgcg cctcccgcca aaggaggctg tcgcgtattt 16141 ccgcgccaaa ggctacaaca tcacctggaa ctggtacgaa caactggcag acgcgcacgc 16201 ccgcgccttt accgttgcca aagccacccg gatggatgtc ctgaccacaa tccgtgagga 16261 agtggagcgg gctgtcagtg aaggcatcac ccgtgaggaa tttacccgca cactggcccc 16321 ccggctgcaa aaactcggat ggtggggaaa acaaatcatc gtggatgcag agggaaacgc 16381 gaaggaaatc gaactgggca gtccgcgtcg tctggcaacc atttataacg tcaatacccg 16441 tacggcgtat ggggcaggac gttatgcgca gatgatgaat accgccgatt tgtacccgta 16501 ctggcagtac gttgccgtta tggatggcag aacacgcccg gaacatgcgc ggctgcacaa 16561 tatggttttc cagtatgacg acattttctg gcagacgcat tacccgccca atggctggaa 16621 ctgccgctgt cgcgtccggg cgctgtctgc ggcccgcatg aaagaactgg ggctacaggt 16681 cagttatggt gcgtcattca tgaatacccg cgaagtggat gccggtacgg atgaaagcac 16741 cggagagatt ttccgcacat cgtcaaccac gtttgacaac ggtcgggtaa aaatgacgcc 16801 agatgtgggc tggtcatata accccggttc ggcggcattt ggtacggatc aggcactgat 16861 tcgaaaactg gtggaagttc gtgacgcgca gctgcgtgag caggttgttc agacgctgaa 16921 taacagccgg gagcgtcagc ttgctttttc cctctggctt aaacgcctgg caggatcacg 16981 tcagaccggt catgaaatcc gggcgctggg gtttatgacg ggatccgtgg ctgaagccgt 17041 atatcagcgc accgggaata tgccagcccg tctgcttgta atgaacggga aaagtctggc 17101 gaccactgct gatgccgccc tgaaaccgga ggatttacag cgtctgccgt cgctgatggc 17161 aaaaccacag gcggtattgt gggacaggga gaaccaccag ttgctgtatg ttgtggcaac 17221 ccgtgacggc acggcccgga ttgtggtcag aacatcacag acagtcggca ggcagaatga 17281 ccgggcggat gtgctggtca gtatcagccg cgtatctgca cagtcgcttg aagccgcgat 17341 tgctgacggg atgattgacg tactggaggg gcatgtggag gtgaataagt gagtcttgat 17401 atgaacgttg ccgtggatgt gcgacgtatt cagcttgcgc tggatgaact gggaaccgtc 17461 acacgtgacc gcgccattcc ccgagtgatg gctgcggcgc tgctgtcatc cacggaacag 17521 gcatttgaac ggcaggcaga cccggataca gggaaaggct gggaagcgtg gagcgattcg 17581 tggctggcat ggcgtcagga tcatggtttt gttccgggca gtatccttac cctgcatggc 17641 gatctggcac gcagtattac caccgattat ggacaggatt atgccctgat tggttcgcca 17701 aaaatttatg ctgccattca tcagtggggc ggtacacccg atatggcacc ccgcccggca 17761 ggcgttcctg cgcgtcctta tatggggctg gataagaccg gggaacagga aatttttgat 17821 gccatcagaa aacgcgtcag cgccgctctg aggcaataaa cagaatcagg cataaaatca 17881 gccgcacaga ttttttaaaa cgcgccacgg gatttttaaa ccggtattta acggtgtatg 17941 aatcccgttt tatcttcctt tcactttctt tctccagtac tcaaatagca taaccccaga 18001 ttttcccgca cctcccgcaa actgactgct cacaaaccat gatgagcagc aggacatgaa 18061 aaaacacgcg attggcattg ccgcactgaa tgcgctgtcc attgacgatg acggctggtg 18121 ccagctattg cctgccggtc atttcagtgc ccgtgatggt cgtccgtttg atgtgacagg 18181 cggtcagggg tggttcatcg acggtgagat tgccgggcgt ctggttgaag gcgttcgcgc 18241 gctcaaccag gacgtgctga ttgattacga gcataaccag ctacgtaagg acaaaggatt 18301 acccccggaa caactcgtcg cggctggctg gtttaacgcc gatgaaatgc agtggcgtga 18361 aggtgaaggg ttatttatcc acccacgctg gacggcggcg gcccagcagc gcatcgatga 18421 cggcgaattt ggttatctct ctgcggtttt tccgtatgac accgccaccg gtgcggtgct 18481 gcaaatccgc cttgctgcgc tgaccaatga ccccggtgcc accggcatga aaaagctgac 18541 cgcactggcg gcagaccttc ccgacatcct tcaacaggag aacaaaccca tgaatgaaac 18601 gctgcgcaag ctgcttgcgc gtcttggggt gacggtcccc gaaaatgcag acatcaccga 18661 tgaacaggca acagcggcac tgaccgcgct ggacacgctg gaaattaatg ccgggaaagt 18721 ggcggcgttg tctgccgaac tggaaaaggc acagaaagcc gccgttgacc tgacgaaata 18781 cgtcccggtg gaaagctaca acgccctgcg tgatgaactg gcacaggcga ctgcacaaag 18841 cgccacagcc agcctgagcg ccgttctgga caaggcggaa caggaaggcc gcattttcaa 18901 aagcgaacgc acttacctgg aacagcttgg cggtcagatt ggcgtggcgg cactgtcggc 18961 acagcttgag aaaaaacagc ccatcgctgc gttatctgcc atgcagacca ccacagcgaa 19021 aattccgtcg caggaaaaaa cggcggtggc ggtgctgtct gctgatgaac aggcagcagt 19081 gaaggcgctc ggcattacag aggctgagta tctgaaaatg aaacaggaac aagaaaaatg 19141 attgtcaccc cggcatccat taaagccctg atgacctcct ggcgtaagga ttttcagggc 19201 ggtcttgaag acgccccgtc gcagtacaac aaaattgcaa tggtggtgaa ctcttccacc 19261 cgcagtaata cgtatggctg gctgggtaaa ttcccgaccc tgaaagaatg ggtgggtaag 19321 cgaaccattc agcaaatgga agcgcatggc tactccatcg ccaataaaac gtttgaaggc 19381 accgtgggta tttcccgtga tgacttcgag gatgacaatc tgggtattta tgcgccgatt 19441 tttcaggaga tgggccgttc tgcggcagtt cagccggatg agctgatttt taaacttctg 19501 aaagatggct ttacccagcc ctgctatgac ggtcagaact tcttcgacaa ggagcatccg 19561 gtatatccga atgtggatgg cacgggcagc gcggttaata cctccaacat cgttgagcag 19621 gacagcttca gcggtctgcc attctatctg ctggactgct cccgcgcagt caaaccgctg 19681 attttccagg agcgccgcaa accggaactg gtcgcacgta cccgtatcga tgacgaccac 19741 gtttttatgg ataacgagtt cctgtttggt gccagcaccc gccgtgctgc cggttacggc 19801 ttctggcaga tggctgtcgc ggtaaaaggc gatctgacgc tggataacct ctggaagggc 19861 tggcaactga tgcgctcttt tgaaggtgat ggcggtaaga aactgggcct gaagccgacg 19921 catatcgttg taccggtcgg gctggaaaaa gccgcagagc aactgcttaa ccgtgaactg 19981 ttcgcggatg gcaataccac cgtctccaac gagatgaagg gcaagctgca actggttgtc 20041 gctgactacc tgtaaccgtc attaaaaggc ggttcagacc gccttttaac ctgatttaaa 20101 ggaaaaggag gagcatgtgt tatgtcaggc acttcgctta attcacaacg gctggacact 20161 tctcgcatca cttgcacggc gattattaaa tgccttcgtc cggtctaccg ccgcgccgga 20221 atcgccttta cgcgtggcga aaacaccgtg gaagtcaccg aagaacaact ggcgatcatt 20281 cgtgccgaca gcgtgctgtc tgtggtatct gcatcgtcag cagaaacgct cgctgaggct 20341 ggggggctgg acgttctggg tgtgggcgat ctgaataccc gtatccgtgc gaccgttgcc 20401 ggtctggata aggcaaaccc ggagcacttt accgcagggg gagaacccaa agtgaaagcc 20461 gtcagtgcgg cgctggggga accggtgagc agtgcgcaaa tcaaagcggc gctggctgag 20521 gcggatgcat gaattacgcg acggtaaatg acctgtgcgc ccgctataca cgtacgcggc 20581 tggatattct cacccgccct aaaacagcgg acgggcagcc ggatgatgcc gtggcagaac 20641 aggcactggc agatgcttcg gcctttattg atggctatct cgccgcgcgc tttgttctgc 20701 cgcttaccgt cgtcccctca ctgctgaaac ggcagtgttg cgtggtggcc tggttttatc 20761 tgaatgaatc acaacccacc gagcagatca ccgcaactta cagggacacg gtgcgctggc 20821 tggaacaggt gcgcgatggc aaaacagatc ccggcgtgga aagcagaacg gcagcgtcgc 20881 cggagggaga ggatcttgtg caggttcagt ctgatccgcc tgttttttca cgaaaacaga 20941 aggggtttat ctgatgctgg aagaaaccga agccgcactg ctggcgcgcg ttcgtgagtt 21001 gtttggggca accctgcggc aggtggaacc ccttaccggc acatggacta atgaggatgt 21061 gcaccgtctc tttctggccc cgccatcggt atttctggca tggatgggct gtggtgaggg 21121 gcgtacgcgt cgggaagttg aaagtcgctg ggcatttttt gtcgtggcgg agttgctgaa 21181 cggggaaccg gtaaaccggc ccggtattta tcagattgtg gagcgactga ttgccggtgt 21241 taacggtcag acgtttggcc cgaccaccgg gatgaggctg acgcaggtca gaaatctttg 21301 tgacgacaac cgtatcaatg ccggtgtggt gctttacggc gttctgttca gtggcacaac 21361 cccgctgccg tccgtagtgg acctggattc gctggatgat tacgagcgtc actggcagac 21421 ctggaaattc ccggacgaaa ccccggaatt tgccgcacat atcaatgtga atcaggaaaa 21481 ggatcatgat gctgaaaatt aaacccgcag cgggaaaagc catccgtgac ccgctcacga 21541 tgaaattact ggcgtctgag ggagaagaaa agccccgtaa cagtttctgg atacgtcgcc 21601 ttgcagccgg tgatgtggtt gaagtcggga gcaccgaaaa cacggcggat gataccgacg 21661 ctgcgccgaa aaaacggagt aaatcgaaat gagcgatatt tcatttaacg cgatcccgtc 21721 agatgttcgc gttcctctga cgtatatcga atttgataac agcaatgccg tcagcggaac 21781 accggctccc cgtcagcgcg tgctgatgtt cgggcaaagc ggaagtaaag ccagtgcggc 21841 accaaacgtg cctgtccgta tccgttccgg ctcacaggcc agtgcggcgt ttggtcaggg 21901 ttccatgctg gcactgatgg cagatgcatt cctgaacgct aaccgcgtgg cggagctgtg 21961 gtgtattccg caggggaacg gcaccggtaa tgctgctgtc ggtgaaattt cactgtcagg 22021 aacggcaggc gaaaacggct cgcttgtgac ttacattgcc ggtcagcgac tggcggtatc 22081 tgtcgcagca ggtgcaacgg gagcggcgct ggctgacctg ctggttgccc gaatcaaagg 22141 ccagcctgat ttaccggtga cggcagaagt tcgcgcagac agcggggatg atgacaccca 22201 tgcagatgtg gttctgagtg caaaatttac gggtgcatta tctgccgtgg acgtgcgctg 22261 gaactattac gcgggtgaaa cgacccctta cgggattatc acggcattca aagccgcctc 22321 cgggaaaaat ggcaacccgg atatcagcgc aagtatcgcg ggaatgggcg atctgcaata 22381 taaatatatt gtgatgccct ataccgatga accgaacctg aatctgttac gcactgaatt 22441 gcaggaacgc tggggaccgg tcaatcaggc tgatggcttt gccgtgacag tgctgtccgg 22501 cacgtatggg gacatttcca cgtttggtgt cagccgtaat gaccatctga tttcctgtat 22561 ggggattgcc ggtgcaccgg aaccgtcata tctgtacgcc gccacactgt gtgccgttgc 22621 cagccaggcg ctttccatcg acccggcgcg tccactccag acgctgacgt tgcccggaag 22681 aatgccgcct gcggtggggg atcgtttcac ctggtcagaa cgtaatgcgc tgctgtttga 22741 cggcatctcc acgtttaacg tgaatgatgg tggtgaaatg cagattgaac gcatgatcac 22801 gatgtaccgc acaaacaagt acggtgacag tgatccgtct tatctgaacg tgaataccat 22861 cgccacgctg agttatctgc gttattcgct gcgaacccgc atcacgcaga aattcccgaa 22921 ctacaagctg gcaagtgatg gcacccgctt tgccaccggt caggccgtgg tgacgccatc 22981 cgtgatcaaa acggagctgc tggcactgtt tgaagaatgg gaaaacgccg gactggtcga 23041 ggatttcgac acgttcaaag aggagctgta tgtggcacgt aacaaggacg acaaagaccg 23101 tcttgatgtg ctgtgcggcc cgaatctgat taaccagttc cgcattttcg cggcacaagt 23161 tcagttcatt ctgtaaggag catttatggc tggaaatcag cgtcaggggg tggcgttcat 23221 ccgtgtcaat ggcatggagc ttgaatcaat ggaaggtgcc tccttcacgc cttccggcat 23281 cacccgtgaa gaagtgaccg gctcgcgggt ttatggctgg aaaggtaaac ctcgtgccgc 23341 aaaagtggaa tgcaaaattc cgggcggtgg tcccatcggg ctggatgaaa ttatcgactg 23401 ggaaaacatc accgtcgaat tccaggctga taccggtgaa acctggatgc tggcgaatgc 23461 ctggcaggcg gatgaaccga aaaacgacgg cggcgaaatt tcgctggtac tgatggcaaa 23521 acaaagcaaa cgcattgcat aaggggaaac aatggacgaa atgaatttag gtcctgaagc 23581 acaggaactg catgacagca ttgtggcaga aattcagtcc ggcgtgctga aactgaaaga 23641 cggcctgccg ttcggcaccg gtgacgagac tgaaatgcag tatgacgtga cgctgcgtga 23701 actgaccgca ggcgatatga ttgatgcaca ggcggctgct gaaaagctgg taatgagcaa 23761 ggaaggtccg gtgctggtca gttccccgtc ccggatggga ctggaaatgc tgcgccgaca 23821 gattgccagc gtgggctgca tcaaaggccc gttgtcgatg gcgctgattc gtaaactgtc 23881 tgtcgatgac ttccagcgtc tgtcgctggc cacagaaatg tacgacatgg ctgtggcggc 23941 atcgctgaca caagaacggg ggcgagtggc tgcggtgccg gaatgatatt gagaaagcgg 24001 cgacagcgat tggcgttatt ctgaaaagtg gcccggaatg ggccttgtct ctcccgctgt 24061 cccgcttttt ccggcactgc cagcaggcta aaaccctctc tcaatatcac cgttaaatca 24121 ggaaatatct atgaccggga aacgcttaaa ggcgtctgtc attattgatc ttaatggcaa 24181 tctttcccgg cgttcccgcc agtattcgaa ccagataaac gccctgtccc gtagcgggca 24241 aagttccctt cgtgccctgc gtatggaggt tgtgcgcgtt tctggtgcca ttgacagaat 24301 gggttcactg tcaacccgta cattccgaat gctgtcagcc ggggcgctgg gtatcgctgg 24361 cgtgggttac accgccaata aactgtttat tggtgcagcc gcccagcgtg agcagcagat 24421 catcgccatg aactcgctgt atcacggcga taaggtcaga gcgcaggcca tgatggcctg 24481 ggcgaagcag aacgcgaagg acaccacgtg ggggctgagt ggtgttctgg atgagatccg 24541 ttcgtcgaaa ggcttcggca tgacggatga acagacgaaa cagttcatca ccatgttgca 24601 ggatcagggg gccatgcacg gctgggattt acccactgca cagggtgcct cactgcaact 24661 gaaacagatg tttgcccgcc agcaaatcac agcggcggat gccaacctgc tgaccggtta 24721 cggtatcaac gtttatcagg cgctggcgga tgcaacggga acggacgtta aaaaaatccg 24781 tgatctgggt acaaagggca agctgggcat gaaatccatt ctgacggtat tcagaacgct 24841 gtcagaacag tcaaaaggtg cacaggccag tgcgatgaac tcctgggacg ggatgttcgc 24901 ccagatggaa gccaacctgc ttgaatttcg cataaaggtg gcaaacagcg gcccctttga 24961 agaaatcaag aacgagatgc gccgggtgct taactggcac gacatggcgg ataaatccgg 25021 ggaacttgac gcactggcag aaaacattgg tcagaaattt ctgaccacgt tcagaacggt 25081 aaaaatctcg gcgcaggaat tatggcgctg gctgaaaccg ggtaaagatg cgctggcatg 25141 ggttgaccag aatattgtca gcctgaaaaa actggctgct gttctggtgt ctgtctggct 25201 tgccaataaa gccctgagag caggctgggc cgtggcaaaa ccctcatggc aggttgccag 25261 ttatccgttt aaaaccgggc gtcgtatgtg gcgctggatg cgaaaccgca aacgtgggca 25321 ggcaggtctg ccggtcccgg atgccatgac gtctgagacg ctgttgcagg gtatcggcat 25381 tcagcgtgtg tttgtcatca actggccccg tggatttggt gactacggga gtggcggcgg 25441 tcgtcgtgta cggagcggcg gaagaatggc cccgctgtta ccccgccagc cgttattgct 25501 gtccgggcca cagccgctgg cattacctgc accacgccct gtactggcat tacccccacc 25561 cggcgtaccg gtaacggcac ggcctgcacc attaccttta cccggtaaat ccggtcttct 25621 gagcaggctt gcaggcagtg ctgccgggca actggttacg ggaacggtcg gaaagctggc 25681 tgatgccggg cgtgccgttg gcgggtggtt ttccggtatc ggaaacaaac ttgcaggcag 25741 tgcgatcggt cgggttgtga caaaaggtgc cggggcgctg ggctggatgg ggaaaggtgc 25801 cggtcgtgcg ttgtcacgtc tggggggccc ggtaatgggg gcactccagc ttgcccccgt 25861 cctgatggat gagcaggcgt caacccatga aaaagcaggc gcaattggca gtacagccgg 25921 tgcatggctc ggcggtgccg ttggcagcct tgccggaccg ctgggtacgg ttgctggtgc 25981 cacgctgggc agtgtcgccg gggagtatct gggcggtttt gtaaccgacc tgtatcagaa 26041 atggacggcc acggataagg aaccgcagga acaaaaagtc aatgcggaag cctcgctgcg 26101 cgtcgaactc ggcgaggggt tacgtctgac cagttcccgc gtcaccgagg atggtatggg 26161 gctgaatatt tacgcgggcg ataactacat tacgggctgg taagaccatg tttgaagatg 26221 ctttaaatgc cgttaatgct gtccgggata aaaccggtgg aggcaggaaa acaaccggca 26281 aaggcacgtt ccgtaacgtg ccgtttctcg tcatcgagga gcaaaaacag gctggcggac 26341 gtcgcctggt taaacgcgag tacccgttac gtgataccgg cggcgtcaat gacctgggga 26401 aaaagcttcg ctcccgtaca ttcagcgcct gcattctgaa cagcaacgca gaaacagcca 26461 gagatgaagc gggtgcgctg atggatgctc ttgatgctcc gggtagcggt gagctggtac 26521 atcctgattt cggcactgtg gacgtcatgg tggattcatg ggaatgccgc actaaagcgg 26581 atgaactgaa ttattacgcg ttcaccgtta ccgtttatcc gtcgttgcag gatactgccc 26641 cggacgcaga gacagacacc agtgcagccg taccggcaca ggccgttgct gtaaccggtt 26701 ctctgggaga tacgctgtcc tctgtctggc aaaccgtaaa agatggcact gcggcggcaa 26761 ccgccgtgat ggaagctgta accggtgtca tcgatgatat cagtgatgcg gtggacaatc 26821 tgggggttac gcagactgtc agcggtctga tgggatcgct ttctgcgatg aaaggctctg 26881 tgaccagcct gattaaccag cctgccatgc tggcctcctc gctgatgggg gcgctgtccg 26941 gcgtttcatc gttatgcgat acccggacag cattttccac atggaaccgt ctggcgcagc 27001 gattcgaacg tcgccatgcc gccaccgcag gcagacaggg gacaatcaca acctcgtaca 27061 acagtccggt tgcagaaaaa aatattgcca cactgaacta cgtcatgctg gcagcggcgc 27121 agacataccg ggcagaagct gccagccagg cactgactgc ggcactggat ttcagtcgcc 27181 ggatggataa tgccgcccgt gcacctgtac tggatgcccc gtccaccaca accggcacag 27241 ccagcggggc cagcagcaca tctgctaccg tcacacaggg acagttacag ttaactgcca 27301 taaccccgga cggcggcttt tcacaggtat ccttttcaga cagtggtaca gccacgcccc 27361 cggtatttga aagtgtgtcc gatatcgaaa aaaccactgc catgctgggg gcggcgctgg 27421 atagcgtcat tctgacggca tctgagcagg gtttttcgac agacagtgtt cagcttacgc 27481 aactgcgtct gctggttgtt gccgacctgg aaaaacgcgg gctgcaactg gcgggtagtg 27541 aatcacacca cctgccagaa acgctcccgg caatggttgc actgtaccgg ttcaccggaa 27601 acagccggaa ctggcaacgg ctggcccgca ggaacggtat cagcaacccg ttatttgttc 27661 ccggtggtgt cagtattgag gtgattaatg agtaataccg tcacactgcg agcggatggc 27721 aggctgttta ccggctggac gtcagtctct gtcacccgct cgattgaatc cgtagccgga 27781 tattttgagc tgggggtgaa cgtgccaccg ggcacggatt tatccgggct ggctccgggg 27841 aagaagttca cgctggaaat cggggggcag attgtctgca ccggttatat cgattcacgg 27901 cgacgccaga tgaccgctga cagtatgaaa atcactgtcg ccggacgtga caaaacggct 27961 gacctgattg actgtgctgc cgtttacagt ggcggacagt ggaaaaaccg cacactggag 28021 cagattgcgc gtgacctgtg cgctccttat ggcgttaccg ttcgctggga gctttccgat 28081 aaggaaagtt cggcagcttt tcccggcttc acgctggacc attcagaaac cgtttatgag 28141 gcgctggtgc gtgcctcccg cgcacgcggt gtactgatga ccagcaatgc cgccggagag 28201 ctggtattca gccgggctgc cagcacagcc actgatgagc tggttctcgg agaaaatctg 28261 ctgacactgg attttgagga agacttccgc gaccggttca gcgaatacac cgtcaagggg 28321 tatgcccgcg caaatggtgc tgagggtgat gatattgatg cgaaaagtat cgtatcccgg 28381 aaagggaccg ccactgacag tgatgtgacc cgttacagac cgatgatcat cattgctgac 28441 agcaagatta cggcgaagga tgcacaggcc cgcgccctgc gtgagcaacg ccgcagactg 28501 gcaaaatcca tcacctttga ggcagaaatt gacggatgga ctcgcaagga cgggcaactc 28561 tggatgccga acctgctggt cactattgat gcctcgaaat atgccatcaa aaccacggaa 28621 ttactggtca gcaaagtcac cctgatactg aatgaccagg acgggctgaa aacccgcgtc 28681 agccttgcac cacgcgaagg ctttctggtg ccggttgaaa gcgaccgtaa aaacaggaaa 28741 ggcggcgaca gtaacggcgg tattgatgcg ctggttgaag attattatcg cagacacccg 28801 gagaaaacgc cgccgtggaa agagtaaatg attccgcctt gaaccgcctg ctgacgccgc 28861 tgatgcgtcg tgtgcgcctg atgcttgcgc gcgctgttgt taacgtgatt aacgacgggc 28921 gaaaggttca gaacctgcag gtcggtctgc tggatgatga ggaatccgat gaagtggagc 28981 gcctgcaaaa ttacgggcat ttcagcgttc ccctgccggg cgcagaggcg ctgattgcct 29041 gtgtaggcgc acaacgtgat caggggattg ctgttgtggt ggaagaccgc cgctaccgcc 29101 cgacaaatct tgaaccgggt gatgcaggca tttaccacca cgaggggcat cgtatcaggc 29161 tgacaaagga cggacgctgc atcattacct gtaaaacggt tgaggtttac gctgatgaaa 29221 gtatgaccgt tgacacaccc agaaccacgt ttaccggcga cgttgagatc cagaaaggtc 29281 tgggcgttaa aggtaaaagc cagttcgaca gcaatattac tgccccggat gccatcatca 29341 atggcaaatc gacggataag catatccacc gtggcgacag cggcggaacc acagggccga 29401 tgcaatgacc gatttagcca ttatctggac gaacggacgc ggcgatattg cgcaggatgg 29461 tattgatatg ctgaccgacg acagcctgac aaccgatgtg acaatctctc tgtttaccga 29521 ccggcgcgcg ctggattctg acacgctgcc ggatggttca gatgatcgcc gtggatggtg 29581 gggtgacagt taccgcgacc gccccatcgg ttcgcggttg tggctgttat cacgtgaaaa 29641 agccacgccg gatacgctgg aacgcgccag agggtatgcc gaagaggcgc tggaatggct 29701 gaaaacagcg ggccgggtaa gtgcgattaa cgtcagagcg gaacagttac atcagggctg 29761 gttatacctc tacattgcac tgacattacc ggatggttcc gttattcctt atgagtttaa 29821 agcagcattt aacggggttt aaatggctta ttcaccaccg acattatcat cgctgattgc 29881 ccgtacagaa cagaatattg aacagcgcct gccgggtagc tggcctcagg cccgtgaaaa 29941 aacgctgagt gccattgctt atgctcaggc gggccttgct gccggttgtc acgagcatat 30001 ttcatgggtt ggacggcaga ttatcccgtc gacagcagat gaagatgagt tgctggagca 30061 ctgccggttc tggggcgtgc gccgcaaaca ggcgacagcc gccagcggcc cgctgactgt 30121 caccacatcg gcagcgacca ccattcctgc cggtacacgc tggcagcgtg ctgatggtgt 30181 ggtttacagc ctggctgata ccattgtgat tgaccgtgca ggaacgacgg aaattaccgt 30241 taccgcactg gctgccggtg aagcaggaaa taccggtgag aatacccttt taacgttgat 30301 caccccggtt gcctgtgttg tttccgatgc catcactgta aaagggtttt ccggtggagc 30361 tgatattgag agtgcagcgg agctgctgtc acggctggaa tatcgtgtcc agtatcctcc 30421 gttcggcggt aatcagtttg attacgttcg ctgggcacgt gaagtcagcg gtgttacccg 30481 tgcctggtgt tttccgacat ggaaaggcgg tggcacagtc ggggtgacgt ttgttatgga 30541 taaccggagc aatatttttc cgcaaccggc agacgtggaa cgcgtggcgg attatatcgc 30601 cggtcatact gacccgatca ctggtctgat tgtcggacag cctgacggtg taaatgtcac 30661 ggtatttgcg ccaaaggcaa agccggtaaa tccacggatt tatatatcac cgaagacagc 30721 cgaactgaaa caggctatta ccaacgccat taataccatg ttttttaatg aggtgatgcc 30781 gggcggcgca cttgccccct cccgtattat ccgtgcggtg gcaggtgtta ccggtctgga 30841 tgattttgaa gtgcgtttcc cgacagagat ccagcgttcg gagaatacgg aactcttaac 30901 agcggggaca attgaatggc tgtaacaccc tggcagacgg cctttctgca attactgccg 30961 tcagggcttg cctggaataa aagccccgac agcaaattat ctgcgctggc gcaggccatc 31021 agcgacgtga ttgccactgc ggcggatgat gcgcggcaaa tgctgcggga gcgtttcccg 31081 tccacatccc gctggtatct gggggagtgg gaatcatttc tggggttgcc ggactgtacc 31141 agcgaaaacg gcaccctgtc cgaacgccag cgggctgccg cgaataaaat gcgtatgacc 31201 ggcaatctga gtcggcgctt ttatgaatgg ctggctgcgc agtacggttt taccgtcagg 31261 ctgacggatt ccacagaagg ccagtgggtt acgcaggtca atatttacgg tattaaaaat 31321 tatcgcaacg caacggtgct ggataatgtt ctgacgccgt tacgtgttta tgaatcgggt 31381 gcgctggaat gtttactgga gaaatataaa cccgcgcatc agatttataa atttgtttac 31441 catgacggag ataactaatg ttttatattg ataacgacag cggcgtaacc gtcatgccgc 31501 ccgtatccgc ccagcgtagt gctatcgttc gctggttttc agaaggtgac gggaataatg 31561 ttatcacatg gcccggcatg gactggttta atattgtgca ggcggagtta ttaaacacgc 31621 tggaagaagc cggtattcaa ccggataaaa caaaattaaa ccagcttgca ctgtccatta 31681 aagccattat gagcaataac gcgctgctga taaaaaataa cctcagcgaa attaaaactg 31741 ccggggcatc agcacagcgt acagcacgtg aaaatctgga tatctatgat gccagcctga 31801 acaaaaaagg actcgttcag ctaaccagtg ccactgacag ccccagtgaa acgctggcag 31861 ccaccgcaaa agcggtgaaa attgcaatgg ataatgccaa tgcccgtctg gcaaaagacc 31921 ggaacggagc agatattccc aataagccgc tgtttatcca aaacctcggt ttacaggaaa 31981 cggtaaacag ggctaggaac gccgtgcaaa agaatggcga taccttgtcc ggtgggctta 32041 cttttgaaaa cgactcaatc cttgcctgga ttcgaaatac tgactgggca aagattggat 32101 ttaaaaatga tgccgacagc gacactgatt catacatgtg gtttgaaaca ggcgacaacg 32161 gcaatgaata tttcaaatgg agaagcaaac aaagcaccac aacaaaagac ctgatgaatc 32221 ttaaatggga tgctttgtct gttcttgtca atgccattgt aaatggcgaa gtcatatcaa 32281 aatcagcaaa cggcctccgt attgcttatg gtaattacgg attctttatt cgtaatgatg 32341 gttcaaatac atacttcatg ttgacaaact ccggtgacaa catggggact tataacggat 32401 taaggccatt atggattaat aacgctactg gcgctgtttc gatggggcgt ggtcttaatg 32461 tttcagggga tacactttca gaccgttttg ctattaacag cagtaatggt atgtggattc 32521 agatgcgcga taacaacgct atctttggga aaaatatagt taacactgat agcgctcagg 32581 cgttgcttcg ccagaatcac gccgaccgca aatttatgat tggcggtctg ggaaataagc 32641 aatttggcat ctacatgatt aataactcaa ggacagccaa tggcaccgat ggtcaggcgt 32701 acatggataa taacggaaac tggttatgcg gtgctcaggt cattccggga aattacgcca 32761 actttgactc ccgctacgtg cgtgacgttc ggctgggaac tcagtcactg actggcggct 32821 tgtctcgtga ttacaaggcg ccatccggtc atgttattac aggttttcat accaatggcg 32881 actgggaaat gcagggaggg gatgacaagg tttatatccg tccggttcag aagaatatca 32941 acggtacctg gtataatgta gcgagcgcct gattatgatg catctgaaaa acattaagtc 33001 agaaaatcca aaaactaaag agcaatatca gctaacaaag aattttgatg ttatctggtt 33061 atggtccgaa gacgggaaaa actggtatga agaagtaaat aactttcagg acgacaccat 33121 aaagattgta tacgacgaaa ataatattat tgttgccata accaaagatg cctcaacgct 33181 taatcccgaa ggctttagcg tcgttgagat tccagatata acagccaacc gccgcgctga 33241 tgattcagga aagtggatgt ttaaggatgg agctgtagtt aaacggattt atacggcaga 33301 cgaacagcaa caacaagccg aatcacaaaa ggccgcattg ctttccgaag ctgaatcagt 33361 catccagccg ctggaacgcg ctgtcaggct gaatatggcg acggatgagg aacgcgcacg 33421 actggagtca tgggaacgct acagtgttct ggtcagccgt gtggatacgg caaatcccga 33481 atggccacaa aagcctgaat aaaaattaag gcccgatagc gggccttctc tcattctggt 33541 tgttctggaa acgttactgg caggctggag gtgtctgtgg attcgacctt ctgcgcgtag 33601 agcatccact ctgttaattt ttgtttattc tcgtcggaaa tgatgcccag ccgtagctgt 33661 gagtcccata gctgtgtttt atccctgacg agctgtagca ggctttgctt ttcattttcc 33721 gcttgttgcc tctgctcttc ctcggtataa gttcgcttta tcactacacc atctttgaac 33781 atccatttac ccgaaatatc agcccggcga tttgctgtaa tatcaggaac ctcaacgacg 33841 tttgcgcctt ctggattaat tgctgaaaca tccttttcaa tacaaataat aacgccgttg 33901 tggtcataga ccattttcaa cgtatcaggc tgaaagttct tttgttcctc ataccagttt 33961 ttcccatcct ctgtataaag ccatttgatg ttaaattgtt tcgttagctg gtattgctct 34021 tttgttttag ggttgccagc agtaatattt tttaagtgca tcataattaa atactccccg 34081 cgttatacca cgttccatta atgcaatact gaattggcct tgcctgagtt gtatcaatta 34141 attcatcacg gtttccgtta actgaacccg taacgacata acctgacctg tcagaccagc 34201 cgggaccatt ccatgtctga acagatgaca gaccgccaag acgaatacct gtaataaacc 34261 ttgagttaca ttctgcctgc gtatatgcac caacatctcc cgctgatggc ttacgtgttg 34321 tggtgtaaat ttctgaccag tcagcttcga atccgtaacc atcacgcgct gaacgataaa 34381 aaataccgcc gttcctgtaa ttcacacgga actgtacggc agggcaactc cccgtattca 34441 tattgaagtg gaggattaat gtcgatgcgc caccaatatt tgcgttatag accccgctat 34501 tccagttcca gccaacagct ttatcatttc cgacagtgct tcctgtttgt cctaaagcaa 34561 atgcaggctg ctggtttttc gtgttgtagt ctcgtcgcca gccaggagca taagcatcac 34621 catgattaat ataagtgaat tgagcgttag tgattccgcc gccgctggac gtactcggcg 34681 tagtaacgcg tatggtcatt gcgccgcgag tgccaataac ttccaccaca gcacctgcaa 34741 gacaaatatt tccgcaacct gtatctgtaa tgaccttatt gtttgcataa gcccatgagc 34801 ctttgcacat ccagtaagga tggttaaatg ccccctgact ctccagccac gaaataaatt 34861 gtgcggttgt ccagacctga ctatcgccac caatattcag ccatgcgcta tatgcgcgac 34921 aggccccaat atttttggtg aaggtatctt ttcctggaat atctgcgccg ttctggtttt 34981 gctgtaatgc gccagaagcc tgatttaccg tttcctgtaa accgaggttt tggataatgg 35041 tcgattttgg cacgcatggc atgattggcg cttttaaaca ggagatccag agtgctgatt 35101 ggctatgtaa gggtatcaac aaatgaccag aatacagacc tgcaacgaaa cgctcttgtt 35161 tgtgcaggat gtgaacaaat atttgaagat aaattaagcg gaacaaggac agaccgaccg 35221 ggattaaaac gcgctttaaa gcgccttcaa aaaggtgaca cactggttgt ctggaaactg 35281 gatcgcctcg ggcgaagcat gaaacatttg atttctctcg taggggaatt acgagagcga 35341 gggattaatt ttcgcagtct tactgacagt attgatacgt catctccaat ggggcgtttt 35401 ttcttccacg ttatgggtgc cctggctgaa atggaacgag aactaattat cgagcgaacg 35461 atggctggac ttgctgccgc cagaaataaa ggccgtattg gtgggcgacc acctaaacta 35521 accaaagcgg aatgggagca ggccgggcgt ttattagcac aaggaatccc ccgcaagcag 35581 gttgcattga tctacgatgt ggccctgtca actctgtata aaaaacaccc cgcgaaacga 35641 gcgcatatag aaaacgacga tcgaatcaat taaatcgatc ggtaatacag atcgattatg 35701 ccccaataac cacactcaac ccatgatgtt ttttaagata gtggcgaatt gatgcaaagg 35761 aggtgagatg aaatcaattc gctgtaaaaa ctgcaacaaa ctgttattta aggcggattc 35821 ctttgatcac attgaaatca ggtgtccgcg ttgcaaacgt cacatcataa tgctgaatgc 35881 ctgcgagcat cccacggaga aacattgtgg gaaaagagaa aaaatcacgc attctgacga 35941 aaccgtgcgt tattgagtat gaaggccaga ttgttggcta tggttcaaag gagctgcgcg 36001 ttgaaaccat atcctgctgg ctggcccgca caattattca gacaaagcac tattcccgcc 36061 gttttgtgaa taactcttac cttcacctgg gggtattcag cggacgcgat ctggttggcg 36121 ttctccagtg gggatatgcc cttaacccca actcaggtcg tcgtgtcgtg cttgaaacgg 36181 ataaccgggg ctatatggag ttgaaccgca tgtggctaca cgacgacatg ccccgcaact 36241 ctgaatcacg ggccatcagc tacgcgctga aagttatcag attactgtat ccgtcagtgg 36301 agtgggttca gtcctttgca gatgaacgct gcggacgcgc aggcgttgtg tatcaggcgt 36361 cgaattttga ttttattggc agtcatgaaa gtacgttcta cgagctggat ggtgagtggt 36421 atcacgagat aacgatgaac gcgattaagc gaggtggaca acgaggcgtg tatttacggg 36481 ctaataaaga gcgtgccgtg gtacacaaat ttaatcagta tcgctacatc agattcctga 36541 acaaacgagc aaggaagcgg ctaaatacca aactattcaa ggttcagcca taccctaagt 36601 gatccccatg taatgaataa aaagcagtaa ttaatacatc tgtttcattt gaagcgcgaa 36661 agctaaagtt ttcgcattta tcgtgaaacg ctttcgcgtt tttcgtgcgc cgcttca //