Mercurial > repos > crs4 > prokka
comparison prokka.xml @ 6:3ad7ef0ba385 draft
Use <stdio> because prokka writes some warnings on stderr. Update Orione citation. Update Prokka citation. Support Prokka 1.10. Upgrade dependencies to package_minced_0_1_6, package_barrnap_0_5 and package_tbl2asn_23_7. Added --proteins option. Add <citations>.
author | crs4 |
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date | Mon, 27 Oct 2014 06:45:52 -0400 |
parents | 5c0030f52b83 |
children | f5e44aad6498 |
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5:b9fea998270a | 6:3ad7ef0ba385 |
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1 <tool id="prokka" name="Prokka" version="1.3.0"> | 1 <tool id="prokka" name="Prokka" version="1.4.0"> |
2 <description>prokaryotic genome annotation</description> | 2 <description>prokaryotic genome annotation</description> |
3 <requirements> | 3 <requirements> |
4 <requirement type="package" version="2.2.28">blast+</requirement> | 4 <requirement type="package" version="2.2.28">blast+</requirement> |
5 <requirement type="package" version="3.1b1">hmmer</requirement> | 5 <requirement type="package" version="3.1b1">hmmer</requirement> |
6 <requirement type="package" version="1.2.36">aragorn</requirement> | 6 <requirement type="package" version="1.2.36">aragorn</requirement> |
7 <requirement type="package" version="2.60">prodigal</requirement> | 7 <requirement type="package" version="2.60">prodigal</requirement> |
8 <requirement type="package" version="22.4">tbl2asn</requirement> | 8 <requirement type="package" version="23.7">tbl2asn</requirement> |
9 <requirement type="package" version="20131122">gnu_parallel</requirement> | 9 <requirement type="package" version="20131122">gnu_parallel</requirement> |
10 <requirement type="package" version="0.3">barrnap</requirement> | 10 <requirement type="package" version="0.5">barrnap</requirement> |
11 <requirement type="package" version="1.1">infernal</requirement> | 11 <requirement type="package" version="1.1">infernal</requirement> |
12 <requirement type="package" version="0.1.4">minced</requirement> | 12 <requirement type="package" version="0.1.6">minced</requirement> |
13 <requirement type="package" version="1.8">prokka</requirement> | 13 <requirement type="package" version="1.10">prokka</requirement> |
14 </requirements> | 14 </requirements> |
15 <version_command>prokka --version</version_command> | 15 <version_command>prokka --version</version_command> |
16 <command> | 16 <command> |
17 prokka | 17 prokka |
18 --cpus \${GALAXY_SLOTS:-8} | 18 --cpus \${GALAXY_SLOTS:-8} |
55 --gcode $kingdom.gcode | 55 --gcode $kingdom.gcode |
56 #end if | 56 #end if |
57 #if $usegenus | 57 #if $usegenus |
58 --usegenus | 58 --usegenus |
59 #end if | 59 #end if |
60 #if $proteins | |
61 --proteins $proteins | |
62 #end if | |
60 #if $metagenome | 63 #if $metagenome |
61 --metagenome | 64 --metagenome |
62 #end if | 65 #end if |
63 #if $fast | 66 #if $fast |
64 --fast | 67 --fast |
75 #if $notrna | 78 #if $notrna |
76 --notrna | 79 --notrna |
77 #end if | 80 #end if |
78 $input | 81 $input |
79 </command> | 82 </command> |
83 <!-- Need to use stdio because prokka writes some warnings on stderr (due to the "use warnings" Perl pragma) --> | |
84 <stdio> | |
85 <exit_code range="1:" /> | |
86 </stdio> | |
80 <inputs> | 87 <inputs> |
81 <param name="input" type="data" format="fasta" label="Contigs to annotate" help="FASTA format" /> | 88 <param name="input" type="data" format="fasta" label="Contigs to annotate" help="FASTA format" /> |
82 <param name="locustag" type="text" value="PROKKA" optional="true" label="Locus tag prefix (--locustag)" /> | 89 <param name="locustag" type="text" value="PROKKA" label="Locus tag prefix (--locustag)" /> |
83 <param name="increment" type="integer" value="1" optional="true" label="Locus tag counter increment (--increment)"> | 90 <param name="increment" type="integer" value="1" optional="true" label="Locus tag counter increment (--increment)"> |
84 <validator type="in_range" min="1" /> | 91 <validator type="in_range" min="1" /> |
85 </param> | 92 </param> |
86 <param name="gffver" type="select" label="GFF version (--gffver)"> | 93 <param name="gffver" type="select" label="GFF version (--gffver)"> |
87 <option value="3">3</option> | 94 <option value="3">3</option> |
97 <param name="addgenes" type="boolean" checked="false" label="Add 'gene' features for each 'CDS' feature (--addgenes)" /> | 104 <param name="addgenes" type="boolean" checked="false" label="Add 'gene' features for each 'CDS' feature (--addgenes)" /> |
98 <param name="mincontig" type="integer" value="200" optional="true" label="Minimum contig size (--mincontiglen)" help="NCBI needs 200" /> | 105 <param name="mincontig" type="integer" value="200" optional="true" label="Minimum contig size (--mincontiglen)" help="NCBI needs 200" /> |
99 </when> | 106 </when> |
100 <when value="yes" /> | 107 <when value="yes" /> |
101 </conditional> | 108 </conditional> |
102 <param name="centre" type="text" value="" optional="true" label="Sequencing centre ID (--centre)" /> | 109 <param name="centre" type="text" value="" label="Sequencing centre ID (--centre)" /> |
103 <param name="genus" type="text" value="" optional="true" label="Genus name (--genus)" help="May be used to aid annotation, see --usegenus below" /> | 110 <param name="genus" type="text" value="" label="Genus name (--genus)" help="May be used to aid annotation, see --usegenus below" /> |
104 <param name="species" type="text" value="" optional="true" label="Species name (--species)" /> | 111 <param name="species" type="text" value="" label="Species name (--species)" /> |
105 <param name="strain" type="text" value="" optional="true" label="Strain name (--strain)" /> | 112 <param name="strain" type="text" value="" label="Strain name (--strain)" /> |
106 <param name="plasmid" type="text" value="" optional="true" label="Plasmid name or identifier (--plasmid)" /> | 113 <param name="plasmid" type="text" value="" label="Plasmid name or identifier (--plasmid)" /> |
107 <conditional name="kingdom"> | 114 <conditional name="kingdom"> |
108 <param name="kingdom_select" type="select" label="Kingdom (--kingdom)"> | 115 <param name="kingdom_select" type="select" label="Kingdom (--kingdom)"> |
109 <option value="Archaea">Archaea</option> | 116 <option value="Archaea">Archaea</option> |
110 <option value="Bacteria" selected="true">Bacteria</option> | 117 <option value="Bacteria" selected="true">Bacteria</option> |
111 <option value="Viruses">Viruses</option> | 118 <option value="Viruses">Viruses</option> |
124 <when value="Viruses"> | 131 <when value="Viruses"> |
125 <param name="gcode" type="integer" value="1" min="1" max="23" optional="true" label="Genetic code (transl_table)" /><!-- max should be 25, but prodigal would crash --> | 132 <param name="gcode" type="integer" value="1" min="1" max="23" optional="true" label="Genetic code (transl_table)" /><!-- max should be 25, but prodigal would crash --> |
126 </when> | 133 </when> |
127 </conditional> | 134 </conditional> |
128 <param name="usegenus" type="boolean" checked="false" label="Use genus-specific BLAST database (--usegenus)" help="Will use the BLAST database for the genus specified above, if installed" /> | 135 <param name="usegenus" type="boolean" checked="false" label="Use genus-specific BLAST database (--usegenus)" help="Will use the BLAST database for the genus specified above, if installed" /> |
136 <param name="proteins" type="data" format="fasta" optional="true" label="Optional FASTA file of trusted proteins to first annotate from (--proteins)" /> | |
129 <param name="metagenome" type="boolean" checked="false" label="Improve gene predictions for highly fragmented genomes (--metagenome)" help="Will set --meta option for Prodigal" /> | 137 <param name="metagenome" type="boolean" checked="false" label="Improve gene predictions for highly fragmented genomes (--metagenome)" help="Will set --meta option for Prodigal" /> |
130 <param name="fast" type="boolean" checked="false" label="Fast mode (--fast)" help="Skip CDS /product searching" /> | 138 <param name="fast" type="boolean" checked="false" label="Fast mode (--fast)" help="Skip CDS /product searching" /> |
131 <param name="evalue" type="float" value="1e-06" optional="true" label="Similarity e-value cut-off"> | 139 <param name="evalue" type="float" value="1e-06" optional="true" label="Similarity e-value cut-off"> |
132 <validator type="in_range" min="0" /> | 140 <validator type="in_range" min="0" /> |
133 </param> | 141 </param> |
182 txt | 190 txt |
183 Statistics relating to the annotated features found | 191 Statistics relating to the annotated features found |
184 | 192 |
185 **License and citation** | 193 **License and citation** |
186 | 194 |
187 This Galaxy tool is Copyright © 2013 `CRS4 Srl.`_, Lionel Guy and is released under the `MIT license`_. | 195 This Galaxy tool is Copyright © 2013-2014 `CRS4 Srl.`_, © 2013 Lionel Guy and is released under the `MIT license`_. |
188 | 196 |
189 .. _CRS4 Srl.: http://www.crs4.it/ | 197 .. _CRS4 Srl.: http://www.crs4.it/ |
190 .. _MIT license: http://opensource.org/licenses/MIT | 198 .. _MIT license: http://opensource.org/licenses/MIT |
191 | 199 |
192 If you use this tool in Galaxy, please cite |Cuccuru2013|_. | 200 You can use this tool only if you agree to the license terms of: `Prokka`_. |
193 | |
194 .. |Cuccuru2013| replace:: Cuccuru, G., Orsini, M., Pinna, A., Sbardellati, A., Soranzo, N., Travaglione, A., Uva, P., Zanetti, G., Fotia, G. (2013) Orione, a web-based framework for NGS analysis in microbiology. *Submitted* | |
195 .. _Cuccuru2013: http://orione.crs4.it/ | |
196 | |
197 This tool uses `Prokka`_, which is licensed separately. Please cite Seemann, T. (2013) Prokka: Rapid Prokaryotic Genome Annotation. *Submitted* | |
198 | 201 |
199 .. _Prokka: http://www.vicbioinformatics.com/software.prokka.shtml | 202 .. _Prokka: http://www.vicbioinformatics.com/software.prokka.shtml |
203 | |
204 If you use this tool, please cite: | |
205 | |
206 - |Cuccuru2014|_ | |
207 - |Seemann2014|_. | |
208 | |
209 .. |Cuccuru2014| replace:: Cuccuru, G., Orsini, M., Pinna, A., Sbardellati, A., Soranzo, N., Travaglione, A., Uva, P., Zanetti, G., Fotia, G. (2014) Orione, a web-based framework for NGS analysis in microbiology. *Bioinformatics* 30(13), 1928-1929 | |
210 .. _Cuccuru2014: http://bioinformatics.oxfordjournals.org/content/30/13/1928 | |
211 .. |Seemann2014| replace:: Seemann, T. (2014) Prokka: rapid prokaryotic genome annotation. *Bioinformatics* 30(14), 2068-2069 | |
212 .. _Seemann2014: http://bioinformatics.oxfordjournals.org/content/30/14/2068 | |
200 </help> | 213 </help> |
214 <citations> | |
215 <citation type="doi">10.1093/bioinformatics/btu135</citation> | |
216 <citation type="doi">10.1093/bioinformatics/btu153</citation> | |
217 </citations> | |
201 </tool> | 218 </tool> |