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+Metadata-Version: 1.1
+Name: SeqSero2
+Version: 1.1.0
+Summary: Salmonella serotyping
+Home-page: https://github.com/denglab/SeqSero2/
+Author: Shaokang Zhang, Hendrik C Den-Bakker and Xiangyu Deng
+Author-email: [e-mail], [e-mail], [e-mail]
+License: GPLv2
+Description: # SeqSero2 v1.1.0
+        Salmonella serotype prediction from genome sequencing data.
+        
+        Online version: http://www.denglab.info/SeqSero2
+        
+        # Introduction 
+        SeqSero2 is a pipeline for Salmonella serotype prediction from raw sequencing reads or genome assemblies
+        
+        # Dependencies 
+        SeqSero2 has three workflows:
+        
+        (A) Allele micro-assembly (default). This workflow takes raw reads as input and performs targeted assembly of serotype determinant alleles. Assembled alleles are used to predict serotype and flag potential inter-serotype contamination in sequencing data (i.e., presence of reads from multiple serotypes due to, for example, cross or carryover contamination during sequencing). 
+        
+        Allele micro-assembly workflow depends on:
+        
+        1. Python 3;
+        
+        2. Biopython 1.73;
+        
+        3. [Burrows-Wheeler Aligner v0.7.12](http://sourceforge.net/projects/bio-bwa/files/);
+        
+        4. [Samtools v1.8](http://sourceforge.net/projects/samtools/files/samtools/);
+        
+        5. [NCBI BLAST v2.2.28+](https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastDocs&DOC_TYPE=Download);
+        
+        6. [SRA Toolkit v2.8.0](http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?cmd=show&f=software&m=software&s=software);
+        
+        7. [SPAdes v3.9.0](http://bioinf.spbau.ru/spades);
+        
+        8. [Bedtools v2.17.0](http://bedtools.readthedocs.io/en/latest/);
+        
+        9. [SalmID v0.11](https://github.com/hcdenbakker/SalmID).
+        
+        
+        (B) Raw reads k-mer. This workflow takes raw reads as input and performs rapid serotype prediction based on unique k-mers of serotype determinants. 
+        
+        Raw reads k-mer workflow (originally SeqSeroK) depends on:
+        
+        1. Python 3;
+        2. [SRA Toolkit](http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?cmd=show&f=software&m=software&s=software) (optional, just used to fastq-dump sra files);
+        
+        
+        (C) Genome assembly k-mer. This workflow takes genome assemblies as input and the rest of the workflow largely overlaps with the raw reads k-mer workflow
+        
+        # Installation
+        ### Conda
+        To install the latest SeqSero2 Conda package (recommended):  
+        ```
+        conda install -c bioconda seqsero2=1.1.0
+        ```
+        ### Git
+        To install the SeqSero2 git repository locally:
+        ```
+        git clone https://github.com/denglab/SeqSero2.git
+        cd SeqSero2
+        python3 -m pip install --user .
+        ```
+        ### Other options
+        Third party SeqSero2 installations (may not be the latest version of SeqSero2): \
+        https://github.com/B-UMMI/docker-images/tree/master/seqsero2 \
+        https://github.com/denglab/SeqSero2/issues/13
+        
+        
+        # Executing the code 
+        Make sure all SeqSero2 and its dependency executables are added to your path (e.g. to ~/.bashrc). Then type SeqSero2_package.py to get detailed instructions.
+        
+            Usage: SeqSero2_package.py 
+        
+            -m <string> (which workflow to apply, 'a'(raw reads allele micro-assembly), 'k'(raw reads and genome assembly k-mer), default=a)
+        
+            -t <string> (input data type, '1' for interleaved paired-end reads, '2' for separated paired-end reads, '3' for single reads, '4' for genome assembly, '5' for nanopore fasta, '6'for nanopore fastq)
+        
+            -i <file> (/path/to/input/file)
+        
+            -p <int> (number of threads for allele mode, if p >4, only 4 threads will be used for assembly since the amount of extracted reads is small, default=1) 
+        
+            -b <string> (algorithms for bwa mapping for allele mode; 'mem' for mem, 'sam' for samse/sampe; default=mem; optional; for now we only optimized for default "mem" mode)
+         
+            -d <string> (output directory name, if not set, the output directory would be 'SeqSero_result_'+time stamp+one random number)
+        	
+            -c <flag> (if '-c' was flagged, SeqSero2 will only output serotype prediction without the directory containing log files)
+            
+            -n <string> (optional, to specify a sample name in the report output)
+            
+            -s <flag> (if '-s' was flagged, SeqSero2 will not output header in SeqSero_result.tsv)
+        		    
+            --check <flag> (use '--check' flag to check the required dependencies)
+            
+            -v, --version (show program's version number and exit)
+        	
+        
+        # Examples
+        Allele mode:
+        
+            # Allele workflow ("-m a", default), for separated paired-end raw reads ("-t 2"), use 10 threads in mapping and assembly ("-p 10")
+            SeqSero2_package.py -p 10 -t 2 -i R1.fastq.gz R2.fastq.gz
+        	
+        K-mer mode:
+        
+            # Raw reads k-mer ("-m k"), for separated paired-end raw reads ("-t 2")
+            SeqSero2_package.py -m k -t 2 -i R1.fastq.gz R2.fastq.gz
+        
+            # Genome assembly k-mer ("-t 4", genome assemblies only predicted by the k-mer workflow, "-m k")
+            SeqSero2_package.py -m k -t 4 -i assembly.fasta
+        	
+        # Output 
+        Upon executing the command, a directory named 'SeqSero_result_Time_your_run' will be created. Your result will be stored in 'SeqSero_result.txt' in that directory. And the assembled alleles can also be found in the directory if using "-m a" (allele mode).
+        
+        
+        # Citation
+        Zhang S, Den-Bakker HC, Li S, Dinsmore BA, Lane C, Lauer AC, Fields PI, Deng X. 
+        SeqSero2: rapid and improved Salmonella serotype determination using whole genome sequencing data.
+        **Appl Environ Microbiology. 2019 Sep; 85(23):e01746-19.** [PMID: 31540993](https://aem.asm.org/content/early/2019/09/17/AEM.01746-19.long) 
+        
+        Zhang S, Yin Y, Jones MB, Zhang Z, Deatherage Kaiser BL, Dinsmore BA, Fitzgerald C, Fields PI, Deng X.  
+        Salmonella serotype determination utilizing high-throughput genome sequencing data.  
+        **J Clin Microbiol. 2015 May;53(5):1685-92.** [PMID: 25762776](http://jcm.asm.org/content/early/2015/03/05/JCM.00323-15)
+        
+Keywords: Salmonella serotyping bioinformatics WGS
+Platform: UNKNOWN
+Classifier: Development Status :: 3 - Alpha
+Classifier: License :: OSI Approved :: GNU General Public License v2 (GPLv2)
+Classifier: Programming Language :: Python :: 3
+Classifier: Topic :: Text Processing :: Linguistic