Mercurial > repos > cstrittmatter > test_galtrakr_eurl_vtec_wgs_pt_23
diff scripts/modules/utils.py @ 0:e37910d2c794 draft
planemo upload commit 15239f1674081ab51ab8dd75a9a40cf1bfaa93e8
author | cstrittmatter |
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date | Mon, 20 Jan 2020 15:11:03 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/scripts/modules/utils.py Mon Jan 20 15:11:03 2020 -0500 @@ -0,0 +1,337 @@ +import pickle +import traceback +import shlex +import subprocess +from threading import Timer +import shutil +import time +import functools +import os.path +import sys +import argparse + + +def start_logger(workdir): + time_str = time.strftime("%Y%m%d-%H%M%S") + sys.stdout = Logger(workdir, time_str) + logfile = sys.stdout.getLogFile() + return logfile, time_str + + +class Logger(object): + def __init__(self, out_directory, time_str): + self.logfile = os.path.join(out_directory, str('run.' + time_str + '.log')) + self.terminal = sys.stdout + self.log = open(self.logfile, "w") + + def write(self, message): + self.terminal.write(message) + self.log.write(message) + self.log.flush() + + def flush(self): + pass + + def getLogFile(self): + return self.logfile + + +def checkPrograms(programs_version_dictionary): + print '\n' + 'Checking dependencies...' + programs = programs_version_dictionary + which_program = ['which', ''] + listMissings = [] + for program in programs: + which_program[1] = program + run_successfully, stdout, stderr = runCommandPopenCommunicate(which_program, False, None, False) + if not run_successfully: + listMissings.append(program + ' not found in PATH.') + else: + print stdout.splitlines()[0] + if programs[program][0] is None: + print program + ' (impossible to determine programme version) found at: ' + stdout.splitlines()[0] + else: + if program.endswith('.jar'): + check_version = ['java', '-jar', stdout.splitlines()[0], programs[program][0]] + programs[program].append(stdout.splitlines()[0]) + else: + check_version = [stdout.splitlines()[0], programs[program][0]] + run_successfully, stdout, stderr = runCommandPopenCommunicate(check_version, False, None, False) + if stdout == '': + stdout = stderr + if program == 'wget': + version_line = stdout.splitlines()[0].split(' ', 3)[2] + else: + version_line = stdout.splitlines()[0].split(' ')[-1] + replace_characters = ['"', 'v', 'V', '+'] + for i in replace_characters: + version_line = version_line.replace(i, '') + print program + ' (' + version_line + ') found' + if programs[program][1] == '>=': + program_found_version = version_line.split('.') + program_version_required = programs[program][2].split('.') + if len(program_version_required) == 3: + if len(program_found_version) == 2: + program_found_version.append(0) + else: + program_found_version[2] = program_found_version[2].split('_')[0] + for i in range(0, len(program_version_required)): + if isinstance(program_found_version[i], (int, long)): + if int(program_found_version[i]) < int(program_version_required[i]): + listMissings.append('It is required ' + program + ' with version ' + programs[program][1] + ' ' + programs[program][2]) + else: + if version_line != programs[program][2]: + listMissings.append('It is required ' + program + ' with version ' + programs[program][1] + ' ' + programs[program][2]) + return listMissings + + +def requiredPrograms(): + programs_version_dictionary = {} + programs_version_dictionary['rematch.py'] = ['--version', '>=', '3.2'] + missingPrograms = checkPrograms(programs_version_dictionary) + if len(missingPrograms) > 0: + sys.exit('\n' + 'Errors:' + '\n' + '\n'.join(missingPrograms)) + + +def general_information(logfile, version, outdir, time_str): + # Check if output directory exists + + print '\n' + '==========> patho_typing <==========' + print '\n' + 'Program start: ' + time.ctime() + + # Tells where the logfile will be stored + print '\n' + 'LOGFILE:' + print logfile + + # Print command + print '\n' + 'COMMAND:' + script_path = os.path.abspath(sys.argv[0]) + print sys.executable + ' ' + script_path + ' ' + ' '.join(sys.argv[1:]) + + # Print directory where programme was lunch + print '\n' + 'PRESENT DIRECTORY:' + present_directory = os.path.abspath(os.getcwd()) + print present_directory + + # Print program version + print '\n' + 'VERSION:' + scriptVersionGit(version, present_directory, script_path) + + # Check programms + requiredPrograms() + + return script_path + + +def setPATHvariable(doNotUseProvidedSoftware, script_path): + path_variable = os.environ['PATH'] + script_folder = os.path.dirname(script_path) + # Set path to use provided softwares + if not doNotUseProvidedSoftware: + bowtie2 = os.path.join(script_folder, 'src', 'bowtie2-2.2.9') + samtools = os.path.join(script_folder, 'src', 'samtools-1.3.1', 'bin') + bcftools = os.path.join(script_folder, 'src', 'bcftools-1.3.1', 'bin') + + os.environ['PATH'] = str(':'.join([bowtie2, samtools, bcftools, path_variable])) + + # Print PATH variable + print '\n' + 'PATH variable:' + print os.environ['PATH'] + + +def scriptVersionGit(version, directory, script_path): + print 'Version ' + version + + try: + os.chdir(os.path.dirname(script_path)) + command = ['git', 'log', '-1', '--date=local', '--pretty=format:"%h (%H) - Commit by %cn, %cd) : %s"'] + run_successfully, stdout, stderr = runCommandPopenCommunicate(command, False, 15, False) + print stdout + command = ['git', 'remote', 'show', 'origin'] + run_successfully, stdout, stderr = runCommandPopenCommunicate(command, False, 15, False) + print stdout + os.chdir(directory) + except: + print 'HARMLESS WARNING: git command possibly not found. The GitHub repository information will not be obtained.' + + +def runTime(start_time): + end_time = time.time() + time_taken = end_time - start_time + hours, rest = divmod(time_taken, 3600) + minutes, seconds = divmod(rest, 60) + print 'Runtime :' + str(hours) + 'h:' + str(minutes) + 'm:' + str(round(seconds, 2)) + 's' + return round(time_taken, 2) + + +def timer(function, name): + @functools.wraps(function) + def wrapper(*args, **kwargs): + print('\n' + 'RUNNING {0}\n'.format(name)) + start_time = time.time() + + results = list(function(*args, **kwargs)) # guarantees return is a list to allow .insert() + + time_taken = runTime(start_time) + print('END {0}'.format(name)) + + results.insert(0, time_taken) + return results + return wrapper + + +def removeDirectory(directory): + if os.path.isdir(directory): + shutil.rmtree(directory) + + +def saveVariableToPickle(variableToStore, pickleFile): + with open(pickleFile, 'wb') as writer: + pickle.dump(variableToStore, writer) + + +def extractVariableFromPickle(pickleFile): + with open(pickleFile, 'rb') as reader: + variable = pickle.load(reader) + return variable + + +def trace_unhandled_exceptions(func): + @functools.wraps(func) + def wrapped_func(*args, **kwargs): + try: + func(*args, **kwargs) + except: + print 'Exception in ' + func.__name__ + traceback.print_exc() + return wrapped_func + + +def kill_subprocess_Popen(subprocess_Popen, command): + print 'Command run out of time: ' + str(command) + subprocess_Popen.kill() + + +def runCommandPopenCommunicate(command, shell_True, timeout_sec_None, print_comand_True): + run_successfully = False + if not isinstance(command, basestring): + command = ' '.join(command) + command = shlex.split(command) + + if print_comand_True: + print 'Running: ' + ' '.join(command) + + if shell_True: + command = ' '.join(command) + proc = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE, shell=True) + else: + proc = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE) + + not_killed_by_timer = True + if timeout_sec_None is None: + stdout, stderr = proc.communicate() + else: + timer = Timer(timeout_sec_None, kill_subprocess_Popen, args=(proc, command,)) + timer.start() + stdout, stderr = proc.communicate() + timer.cancel() + not_killed_by_timer = timer.isAlive() + + if proc.returncode == 0: + run_successfully = True + else: + if not print_comand_True and not_killed_by_timer: + print 'Running: ' + str(command) + if len(stdout) > 0: + print 'STDOUT' + print stdout.decode("utf-8") + if len(stderr) > 0: + print 'STDERR' + print stderr.decode("utf-8") + return run_successfully, stdout, stderr + + +def required_length(tuple_length_options, argument_name): + class RequiredLength(argparse.Action): + def __call__(self, parser, args, values, option_string=None): + if len(values) not in tuple_length_options: + msg = 'Option {argument_name} requires one of the following number of arguments: {tuple_length_options}'.format( + argument_name=self.argument_name, tuple_length_options=tuple_length_options) + raise argparse.ArgumentTypeError(msg) + setattr(args, self.dest, values) + return RequiredLength + + +def get_sequence_information(fasta_file, length_extra_seq): + sequence_dict = {} + headers = {} + + with open(fasta_file, 'rtU') as reader: + blank_line_found = False + sequence_counter = 0 + temp_sequence_dict = {} + for line in reader: + line = line.splitlines()[0] + if len(line) > 0: + if not blank_line_found: + if line.startswith('>'): + if len(temp_sequence_dict) > 0: + if temp_sequence_dict.values()[0]['length'] - 2 * length_extra_seq > 0: + sequence_dict[temp_sequence_dict.keys()[0]] = temp_sequence_dict.values()[0] + headers[temp_sequence_dict.values()[0]['header'].lower()] = sequence_counter + else: + print temp_sequence_dict.values()[0]['header'] + ' sequence ignored due to length <= 0' + temp_sequence_dict = {} + + if line[1:].lower() in headers: + sys.exit('Found duplicated sequence headers') + + sequence_counter += 1 + temp_sequence_dict[sequence_counter] = {'header': line[1:].lower(), 'sequence': '', 'length': 0} + else: + temp_sequence_dict[sequence_counter]['sequence'] += line.upper() + temp_sequence_dict[sequence_counter]['length'] += len(line) + else: + sys.exit('It was found a blank line between the fasta file above line ' + line) + else: + blank_line_found = True + + if len(temp_sequence_dict) > 0: + if temp_sequence_dict.values()[0]['length'] - 2 * length_extra_seq > 0: + sequence_dict[temp_sequence_dict.keys()[0]] = temp_sequence_dict.values()[0] + headers[temp_sequence_dict.values()[0]['header'].lower()] = sequence_counter + else: + print temp_sequence_dict.values()[0]['header'] + ' sequence ignored due to length <= 0' + + return sequence_dict, headers + + +def simplify_sequence_dict(sequence_dict): + simple_sequence_dict = {} + for counter, info in sequence_dict.items(): + simple_sequence_dict[info['header']] = info + del simple_sequence_dict[info['header']]['header'] + return simple_sequence_dict + + +def chunkstring(string, length): + return (string[0 + i:length + i] for i in range(0, len(string), length)) + + +def clean_headers_sequences(sequence_dict): + problematic_characters = ["|", " ", ",", ".", "(", ")", "'", "/", ":"] + # print 'Checking if reference sequences contain ' + str(problematic_characters) + '\n' + + headers_changed = False + new_headers = {} + for i in sequence_dict: + if any(x in sequence_dict[i]['header'] for x in problematic_characters): + for x in problematic_characters: + sequence_dict[i]['header'] = sequence_dict[i]['header'].replace(x, '_') + headers_changed = True + new_headers[sequence_dict[i]['header'].lower()] = i + + if headers_changed: + print 'At least one of the those characters was found. Replacing those with _' + '\n' + + return sequence_dict, new_headers