Mercurial > repos > davidvanzessen > argalaxy_tools
diff bla/c/8/e/dataset_c8e48707-5040-4674-8189-5ebffd00b6c5_files/index.html @ 61:f38a058e1a74 draft default tip
planemo upload commit 93519df849f317f4a8fc6167a48a4ee06e6aeff8
| author | rhpvorderman |
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| date | Wed, 30 Apr 2025 12:44:38 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bla/c/8/e/dataset_c8e48707-5040-4674-8189-5ebffd00b6c5_files/index.html Wed Apr 30 12:44:38 2025 +0000 @@ -0,0 +1,739 @@ +<html><head><title>Report on: +</title><script type='text/javascript' src='jquery-1.11.0.min.js'></script> +<link rel='stylesheet' type='text/css' href='pure-min.css'> +<script type='text/javascript' src='tabber.js'></script> +<script type='text/javascript' src='script.js'></script> +<link rel='stylesheet' type='text/css' href='style.css'></head> +<div class='tabber'><div class='tabbertab' title='Gene frequencies'> +<a href='VFPlot.pdf'><img src='VFPlot.png'/></a> +<a href='VPlot.pdf'><img src='VPlot.png'/></a> +<a href='JPlot.pdf'><img src='JPlot.png'/></a> <br /> +<a href='DReadingFrame.pdf'><img src='DReadingFrame.png'/></a> +<html> + +<head> +<meta http-equiv=Content-Type content="text/html; charset=UTF-8"> +<meta name=ProgId content=Word.Document> +<meta name=Generator content="Microsoft Word 14"> +<meta name=Originator content="Microsoft Word 14"> +<link rel=File-List href="Gene%20frequencies%20tab_files/filelist.xml"> +<link rel=themeData href="Gene%20frequencies%20tab_files/themedata.thmx"> +<style> +<!-- + /* Font Definitions */ + @font-face + {font-family:Calibri; + panose-1:2 15 5 2 2 2 4 3 2 4; + mso-font-charset:0; + mso-generic-font-family:swiss; + mso-font-pitch:variable; + mso-font-signature:-536870145 1073786111 1 0 415 0;} + /* Style Definitions */ + p.MsoNormal, li.MsoNormal, div.MsoNormal + {mso-style-unhide:no; + mso-style-qformat:yes; + mso-style-parent:""; + margin-top:0in; + margin-right:0in; + margin-bottom:10.0pt; + margin-left:0in; + line-height:115%; + mso-pagination:widow-orphan; + font-size:11.0pt; + font-family:"Calibri","sans-serif"; + mso-fareast-font-family:"Times New Roman"; + mso-fareast-theme-font:minor-fareast; + mso-bidi-font-family:"Times New Roman";} +p.MsoNoSpacing, li.MsoNoSpacing, div.MsoNoSpacing + {mso-style-priority:1; + mso-style-unhide:no; + mso-style-qformat:yes; + margin:0in; + margin-bottom:.0001pt; + mso-pagination:widow-orphan; + font-size:11.0pt; + font-family:"Calibri","sans-serif"; + mso-fareast-font-family:"Times New Roman"; + mso-fareast-theme-font:minor-fareast; + mso-bidi-font-family:"Times New Roman";} +p.msochpdefault, li.msochpdefault, div.msochpdefault + {mso-style-name:msochpdefault; + mso-style-unhide:no; + mso-margin-top-alt:auto; + margin-right:0in; + mso-margin-bottom-alt:auto; + margin-left:0in; + mso-pagination:widow-orphan; + font-size:12.0pt; + font-family:"Calibri","sans-serif"; + mso-fareast-font-family:"Times New Roman"; + mso-fareast-theme-font:minor-fareast; + mso-bidi-font-family:"Times New Roman";} +p.msopapdefault, li.msopapdefault, div.msopapdefault + {mso-style-name:msopapdefault; + mso-style-unhide:no; + mso-margin-top-alt:auto; + margin-right:0in; + margin-bottom:10.0pt; + margin-left:0in; + line-height:115%; + mso-pagination:widow-orphan; + font-size:12.0pt; + font-family:"Times New Roman","serif"; + mso-fareast-font-family:"Times New Roman"; + mso-fareast-theme-font:minor-fareast;} +.MsoChpDefault + {mso-style-type:export-only; + mso-default-props:yes; + font-size:10.0pt; + mso-ansi-font-size:10.0pt; + mso-bidi-font-size:10.0pt; + mso-ascii-font-family:Calibri; + mso-hansi-font-family:Calibri;} +.MsoPapDefault + {mso-style-type:export-only; + margin-bottom:10.0pt; + line-height:115%;} +@page WordSection1 + {size:8.5in 11.0in; + margin:1.0in 1.0in 1.0in 1.0in; + mso-header-margin:.5in; + mso-footer-margin:.5in; + mso-paper-source:0;} +div.WordSection1 + {page:WordSection1;} +--> +</style> +<!--[if gte mso 10]> +<style> + /* Style Definitions */ + table.MsoNormalTable + {mso-style-name:"Table Normal"; + mso-tstyle-rowband-size:0; + mso-tstyle-colband-size:0; + mso-style-noshow:yes; + mso-style-priority:99; + mso-style-parent:""; + mso-padding-alt:0in 5.4pt 0in 5.4pt; + mso-para-margin-top:0in; + mso-para-margin-right:0in; + mso-para-margin-bottom:10.0pt; + mso-para-margin-left:0in; + line-height:115%; + mso-pagination:widow-orphan; + font-size:10.0pt; + font-family:"Calibri","sans-serif";} +</style> +<![endif]--><!--[if gte mso 9]><xml> + <o:shapedefaults v:ext="edit" spidmax="1026"/> +</xml><![endif]--><!--[if gte mso 9]><xml> + <o:shapelayout v:ext="edit"> + <o:idmap v:ext="edit" data="1"/> + </o:shapelayout></xml><![endif]--> +</head> + +<body lang=EN-US style='tab-interval:.5in'> + +<div class=WordSection1> + +<p class=MsoNoSpacing style='text-align:justify'><b><u><span lang=EN-GB +style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-ansi-language: +EN-GB'>Gene frequencies tab</span></u></b></p> + +<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align: +justify;line-height:normal'><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif";mso-ansi-language:EN-GB'>This tab gives +information on the frequency of V, D and J gene usage. <o:p></o:p></span></p> + +<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align: +justify;line-height:normal'><o:p> </o:p></p> + +<p class=MsoNoSpacing style='text-align:justify'><b><span lang=EN-GB +style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-ansi-language: +EN-GB'>Graphs</span></b></p> + +<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align: +justify;line-height:normal'><u><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif";mso-ansi-language:EN-GB'>Distribution of +V gene families<o:p></o:p></span></u></p> + +<p class=MsoNoSpacing style='text-align:justify'><a name="OLE_LINK121"></a><a +name="OLE_LINK123"></a><a name="OLE_LINK122"></a><span style='mso-bookmark: +OLE_LINK121'><span lang=EN-GB style='font-size:12.0pt;font-family:"Times New Roman","serif"; +mso-ansi-language:EN-GB'>Bar graph showing the frequency of V gene family usage +in all uploaded donors. </span></span><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif";mso-ansi-language:EN-GB'><o:p></o:p></span></p> + +<p class=MsoNoSpacing style='text-align:justify'><o:p> </o:p></p> + +<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align: +justify;line-height:normal'><u><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif";mso-ansi-language:EN-GB'>Relative +frequency of V gene usage<o:p></o:p></span></u></p> + +<p class=MsoNoSpacing style='text-align:justify'><a name="OLE_LINK124"></a><a +name="OLE_LINK126"></a><a name="OLE_LINK125"></a><span style='mso-bookmark: +OLE_LINK124'><span lang=EN-GB style='font-size:12.0pt;font-family:"Times New Roman","serif"; +mso-ansi-language:EN-GB'>Bar graph showing the frequency of V genes in all +uploaded donors. The order of the V genes on the x-axis can be altered by +altering the “<span style='color:black;background:white'>Order of V(D)J genes +in graphs” filter on the main page of the Immune repertoire pipeline.</span></span></span><span +lang=EN-GB style='font-size:12.0pt;font-family:"Times New Roman","serif"; +color:black;background:white;mso-ansi-language:EN-GB'><o:p></o:p></span></p> + +<p class=MsoNoSpacing style='text-align:justify'><o:p> </o:p></p> + +<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align: +justify;line-height:normal'><u><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif";mso-ansi-language:EN-GB'>Relative +frequency of D gene usage<o:p></o:p></span></u></p> + +<p class=MsoNoSpacing style='text-align:justify'><span lang=EN-GB +style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-ansi-language: +EN-GB'>Bar graph showing the frequency of D genes in all uploaded donors. The +order of the D genes on the x-axis can be altered by altering the “<span +style='color:black;background:white'>Order of V(D)J genes in graphs” filter on +the main page of the Immune repertoire pipeline.<o:p></o:p></span></span></p> + +<p class=MsoNoSpacing style='text-align:justify'><o:p> </o:p></p> + +<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align: +justify;line-height:normal'><u><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif";mso-ansi-language:EN-GB'>Relative +frequency of J gene usage<o:p></o:p></span></u></p> + +<p class=MsoNoSpacing style='text-align:justify'><span lang=EN-GB +style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-ansi-language: +EN-GB'>Bar graph showing the frequency of J genes in all uploaded donors. The +order of the J genes on the x-axis can be altered by altering the “<span +style='color:black;background:white'>Order of V(D)J genes in graphs” filter on +the main page of the Immune repertoire pipeline.<o:p></o:p></span></span></p> + +<p class=MsoNoSpacing style='text-align:justify'><o:p> </o:p></p> + +<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align: +justify;line-height:normal'><u><span style='font-size:12.0pt;font-family:"Times New Roman","serif"'>D +reading frame<o:p></o:p></span></u></p> + +<p class=MsoNoSpacing style='text-align:justify'><span lang=EN-GB +style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-ansi-language: +EN-GB'>Bar graph visualizing the frequency by which each D reading frame is +used in each donor.</span></p> + +</div> + +</body> + +</html> +</div> +<div class='tabbertab' title='CDR3 Characteristics'> +<a href='CDR3LengthPlot.pdf'><img src='CDR3LengthPlot.png'/></a><br /> +<a href='AAComposition.pdf'><img src='AAComposition.png'/></a> +<table class='pure-table pure-table-striped'> +<thead><tr><th>Donor</th><th>Median CDR3 Length</th></tr></thead> +</table> +<html> + +<head> +<meta http-equiv=Content-Type content="text/html; charset=UTF-8"> +<meta name=Generator content="Microsoft Word 14 (filtered)"> +<style> +<!-- + /* Font Definitions */ + @font-face + {font-family:Calibri; + panose-1:2 15 5 2 2 2 4 3 2 4;} + /* Style Definitions */ + p.MsoNormal, li.MsoNormal, div.MsoNormal + {margin-top:0in; + margin-right:0in; + margin-bottom:10.0pt; + margin-left:0in; + line-height:115%; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +.MsoChpDefault + {font-family:"Calibri","sans-serif";} +.MsoPapDefault + {margin-bottom:10.0pt; + line-height:115%;} +@page WordSection1 + {size:8.5in 11.0in; + margin:1.0in 1.0in 1.0in 1.0in;} +div.WordSection1 + {page:WordSection1;} +p.MsoNoSpacing, li.MsoNoSpacing, div.MsoNoSpacing + {mso-style-priority:1; + mso-style-unhide:no; + mso-style-qformat:yes; + margin:0in; + margin-bottom:.0001pt; + mso-pagination:widow-orphan; + font-size:11.0pt; + font-family:"Calibri","sans-serif"; + mso-fareast-font-family:"Times New Roman"; + mso-fareast-theme-font:minor-fareast; + mso-bidi-font-family:"Times New Roman";} +--> +</style> + +</head> + +<body lang=EN-US> + +<div class=WordSection1> + +<p class=MsoNormalCxSpFirst style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><b><u><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>CDR3 characteristics tab</span></u></b></p> + +<p class=MsoNormalCxSpMiddle style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>This tab gives information on the +different characteristics of the CDR3.</span></p> + +<p class=MsoNormalCxSpMiddle style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><b><span lang=EN-GB style='font-size: +12.0pt;font-family:"Times New Roman","serif"'>Graphs</span></b></p> + +<p class=MsoNoSpacing style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><u><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>length distribution of CDR3</span></u></p> + +<p class=MsoNoSpacing style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>Graph visualizing for each donor the +distribution of the CDR3 length in amino acids between all sequences.</span></p> + +<p class=MsoNormalCxSpMiddle style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><u><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>Amino acid composition of CDR3</span></u></p> + +<p class=MsoNoSpacing style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>Bar graph visualizing for each donor how +frequent each amino acid is used in the CDR3 of all sequences. The red colour +in the background indicate positively charged amino acids, and the blue colour +in the background indicate negatively charged amino acids. The amino acids are +ordered from hydrophilic (left) to hydrophobic (right). </span></p> + +<p class=MsoNormalCxSpMiddle style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><b><span lang=EN-GB style='font-size: +12.0pt;font-family:"Times New Roman","serif"'>Table</span></b></p> + +<p class=MsoNoSpacing style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><span lang=EN-GB style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>Table showing for each donor the median +CDR3 length.</span></p> + +<p class=MsoNormal><span lang=EN-GB> </span></p> + +</div> + +</body> + +</html> +</div> +<div class='tabbertab' title='Heatmaps'><div class='tabber'> +<html> + +<head> +<meta http-equiv=Content-Type content="text/html; charset=UTF-8"> +<meta name=Generator content="Microsoft Word 14 (filtered)"> +<style> +<!-- + /* Font Definitions */ + @font-face + {font-family:Calibri; + panose-1:2 15 5 2 2 2 4 3 2 4;} + /* Style Definitions */ + p.MsoNormal, li.MsoNormal, div.MsoNormal + {margin-top:0in; + margin-right:0in; + margin-bottom:10.0pt; + margin-left:0in; + line-height:115%; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +p.MsoNoSpacing, li.MsoNoSpacing, div.MsoNoSpacing + {margin:0in; + margin-bottom:.0001pt; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +.MsoChpDefault + {font-family:"Calibri","sans-serif";} +.MsoPapDefault + {margin-bottom:10.0pt; + line-height:115%;} +@page WordSection1 + {size:8.5in 11.0in; + margin:1.0in 1.0in 1.0in 1.0in;} +div.WordSection1 + {page:WordSection1;} +--> +</style> + +</head> + +<body lang=EN-US> + +<div class=WordSection1> + +<p class=MsoNormalCxSpFirst style='text-align:justify'><span style='font-size: +12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>In this tab +heatmaps are provided showing the frequency of different combinations V and D, +D and J and V and J genes according together. For each donor a subtab with all +three heatmaps is provided.</span></p> + +<p class=MsoNormal> </p> + +</div> + +</body> + +</html> +</div></div> +<div class='tabbertab' title='Compare Heatmaps'><table class='pure-table pure-table-striped'><thead><tr><th>ID</th><th>Include</th></tr></thead> +</table><div name='comparisonarea'> +<table><tr id='comparison_table_vd'></tr></table> +<table><tr id='comparison_table_vj'></tr></table> +<table><tr id='comparison_table_dj'></tr></table> +<html> + +<head> +<meta http-equiv=Content-Type content="text/html; charset=UTF-8"> +<meta name=Generator content="Microsoft Word 14 (filtered)"> +<style> +<!-- + /* Font Definitions */ + @font-face + {font-family:Calibri; + panose-1:2 15 5 2 2 2 4 3 2 4;} + /* Style Definitions */ + p.MsoNormal, li.MsoNormal, div.MsoNormal + {margin-top:0in; + margin-right:0in; + margin-bottom:10.0pt; + margin-left:0in; + line-height:115%; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +p.MsoNoSpacing, li.MsoNoSpacing, div.MsoNoSpacing + {margin:0in; + margin-bottom:.0001pt; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +.MsoChpDefault + {font-family:"Calibri","sans-serif";} +.MsoPapDefault + {margin-bottom:10.0pt; + line-height:115%;} +@page WordSection1 + {size:8.5in 11.0in; + margin:1.0in 1.0in 1.0in 1.0in;} +div.WordSection1 + {page:WordSection1;} +--> +</style> + +</head> + +<body lang=EN-US> + +<div class=WordSection1> + +<p class=MsoNormalCxSpFirst style='text-align:justify'><span style='font-size: +12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The tab allows +you to compare the VD, VJ and DJ heatmaps of multiple donors to each other. </span></p> + +<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%;font-family: +"Times New Roman","serif"'>By ticking the include box of a donor, the three +heatmaps of this donor is visualized underneath eachother. By clicking the +include box of multiple replicates the heatmaps of these replicates are visualized +next to each other allowing easy comparison of heatmaps. </span></p> + +<p class=MsoNormal> </p> + +</div> + +</body> + +</html> +</div></div> +<div class='tabbertab' title='Circos'><div class='tabber'> +<html> + +<head> +<meta http-equiv=Content-Type content="text/html; charset=UTF-8"> +<meta name=Generator content="Microsoft Word 14 (filtered)"> +<style> +<!-- + /* Font Definitions */ + @font-face + {font-family:Calibri; + panose-1:2 15 5 2 2 2 4 3 2 4;} + /* Style Definitions */ + p.MsoNormal, li.MsoNormal, div.MsoNormal + {margin-top:0in; + margin-right:0in; + margin-bottom:10.0pt; + margin-left:0in; + line-height:115%; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +p.MsoNoSpacing, li.MsoNoSpacing, div.MsoNoSpacing + {margin:0in; + margin-bottom:.0001pt; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +.MsoChpDefault + {font-family:"Calibri","sans-serif";} +.MsoPapDefault + {margin-bottom:10.0pt; + line-height:115%;} +@page WordSection1 + {size:8.5in 11.0in; + margin:1.0in 1.0in 1.0in 1.0in;} +div.WordSection1 + {page:WordSection1;} +--> +</style> + +</head> + +<body lang=EN-US> + +<div class=WordSection1> + +<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%;font-family: +"Times New Roman","serif"'>Martin Krzywinski, Jacqueline Schein, İnanç +Birol, Joseph Connors, Randy Gascoyne, Doug Horsman, Steven J. Jones and Marco +A. Marra (2009). Circos: An information aesthetic for comparative genomics. In <i>Genome +Research 10.1101/gr.092759.109</i>.</span></p> + +<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%;font-family: +"Times New Roman","serif"'>Circos plot showing the V-D, V-J, and D-J gen usage. +The thicker the line the more frequent this gene combination is used.</span></p> + +</div> + +</body> + +</html> +</div></div> +<div class='tabbertab' title='Downloads'> +<table class='pure-table pure-table-striped'> +<thead><tr><th>Description</th><th>Link</th></tr></thead> +<tr><td>All outputs below in a zip file</td><td><a href='all_outputs.zip'>Download</a></td></tr> +<tr><td>The filtered dataset</td><td><a href='allUnique.txt'>Download</a></td></tr> +<tr><td colspan='2' style='background-color:#E0E0E0;'>Gene frequencies</td></tr> +<tr><td>The dataset used to generate the distribution of V gene families graph</td><td><a href='VFFrequency.txt'>Download</a></td></tr> +<tr><td>The dataset used to generate the relative frequency of V gene usage graph</td><td><a href='VFrequency.txt'>Download</a></td></tr> +<tr><td>The dataset used to generate the relative frequency of J gene usage graph</td><td><a href='JFrequency.txt'>Download</a></td></tr> +<tr><td>The dataset used to generate the relative frequency of the D reading frame graph</td><td><a href='DReadingFrame.txt'>Download</a></td></tr> +<tr><td colspan='2' style='background-color:#E0E0E0;'>CDR3 Characteristics</td></tr> +<tr><td>The dataset used to generate the CDR3 length frequency graph</td><td><a href='CDR3LengthPlot.txt'>Download</a></td></tr> +<tr><td>The dataset used to generate the Amino Acid Composition in the CDR3 graph</td><td><a href='AAComposition.txt'>Download</a></td></tr> +<tr><td colspan='2' style='background-color:#E0E0E0;'>Heatmaps</td></tr> +<tr><td colspan='2' style='background-color:#E0E0E0;'>Circos</td></tr> +<tr><td colspan='2' style='background-color:#E0E0E0;'>Clonality</td></tr> +<tr><td>The dataset used to calculate clonality score (Unique based on clonaltype, none)</td><td><a href='clonalityComplete.txt'>Download</a></td></tr> +</table> +<html> + +<head> +<meta http-equiv=Content-Type content="text/html; charset=UTF-8"> +<meta name=Generator content="Microsoft Word 14 (filtered)"> +<style> +<!-- + /* Font Definitions */ + @font-face + {font-family:Calibri; + panose-1:2 15 5 2 2 2 4 3 2 4;} + /* Style Definitions */ + p.MsoNormal, li.MsoNormal, div.MsoNormal + {margin-top:0in; + margin-right:0in; + margin-bottom:10.0pt; + margin-left:0in; + line-height:115%; + font-size:11.0pt; + font-family:"Calibri","sans-serif";} +.MsoChpDefault + {font-family:"Calibri","sans-serif";} +.MsoPapDefault + {margin-bottom:10.0pt; + line-height:115%;} +@page WordSection1 + {size:8.5in 11.0in; + margin:1.0in 1.0in 1.0in 1.0in;} +div.WordSection1 + {page:WordSection1;} +--> +</style> + +</head> + +<body lang=EN-US> + +<div class=WordSection1> + +<p class=MsoNormalCxSpFirst style='text-align:justify'><b><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Downloads +tab</span></u></b></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><b><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Description</span></b></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +filtered dataset:</span></u><span style='font-size:12.0pt;line-height:115%; +font-family:"Times New Roman","serif"'> <a name="OLE_LINK15"></a><a +name="OLE_LINK14">Allows you to download information on V D and J gene usage, +the junctions and the amino acid sequence of the sequences that passed the chosen +filter settings. Note: this file contains only 1 sequences of a replicate +sequence. </a></span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><b><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Gene +frequencies</span></b></p> + +<p class=MsoNormalCxSpMiddle style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><u><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>The dataset used to generate the distribution +of V gene families graph:</span></u><span style='font-size:12.0pt;font-family: +"Times New Roman","serif"'> Downloads a table with information used to generate +the distribution of V gene families graph.</span></p> + +<p class=MsoNormalCxSpMiddle style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><u><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>The dataset used to generate the +distribution of D gene families graph: </span></u><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>Downloads a table with information used +to generate the distribution of D gene families graph.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +dataset used to generate the relative frequency of V gene usage graph:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data used to generate the relative frequency of V gene usage +graph. In addition information on the chromosomal order of the V genes can be +found.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +dataset used to generate the relative frequency of D gene usage graph:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data used to generate the relative frequency of D gene usage +graph. In addition information on the chromosomal order of the D genes can be +found.<u> </u></span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +dataset used to generate the relative frequency of J gene usage graph: </span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Downloads +the data used to generate the relative frequency of J gene usage graph. In +addition information on the chromosomal order of the J genes can be found.<u> </u></span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=EN-GB +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"; +color:black'>The dataset used to generate the relative frequency of the D +reading frame graph</span></u><span lang=EN-GB style='font-size:12.0pt; +line-height:115%;font-family:"Times New Roman","serif";color:black'>: Downloads +the data used to generate the relative frequency of the D reading frame. </span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><b><span lang=EN-GB +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"; +color:black'>CDR3 characteristics</span></b></p> + +<p class=MsoNormalCxSpMiddle style='margin-bottom:0in;margin-bottom:.0001pt; +text-align:justify;line-height:normal'><u><span style='font-size:12.0pt; +font-family:"Times New Roman","serif"'>The dataset used to generate the CDR3 +length frequency graph:</span></u><span style='font-size:12.0pt;font-family: +"Times New Roman","serif"'> Downloads a table with information used for the +length distribution of CDR3 plot. </span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +dataset used to generate the Amino Acid Composition in the CDR3 graph:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the frequency of the different amino acids in the CDR3 of all +filtered sequences. This data set is used for the generation of the Amino Acid +Composition in the CDR3 graph. In addition the order of amino acids based on hydrophobicity +in included. </span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><b><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Heatmaps</span></b></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +data used to generate the VD heatmap for “donor name”:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data set used for the generation of the VD heatmap. For each +uploaded donor a separate download is generated.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +data used to generate the VJ heatmap for “donor name”:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data set used for the generation of the VJ heatmap. For each +uploaded donor a separate download is generated.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +data used to generate the DJ heatmap for “sample name”:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data set used for the generation of the DJ heatmap. For each +uploaded donor a separate download is generated.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><b><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Circos</span></b></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +data used to generate the VD circus plots for “donor name”:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data set used for the generation of the VD heatmap. For each +uploaded donor a separate download is generated.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +data used to generate the VJ circus plots for “donor name”:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data set used for the generation of the VJ heatmap. For each +uploaded donor a separate download is generated.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +data used to generate the DJ circus plots for “sample name”:</span></u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> +Downloads the data set used for the generation of the DJ heatmap. For each +uploaded donor a separate download is generated.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><b><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Clonality</span></b></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>The +dataset used to calculate clonality score: </span></u><span style='font-size: +12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Allows you to +download information on V D and J gene usage, the junctions and the amino acid +sequence of the sequences that passed the chosen filter settings. Note: this +file contains all the replicate sequences form the different input files.</span></p> + +<p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span +style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Sequences +that are present in more than one replicate:</span></u><span style='font-size: +12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> Allows you to +download a table containing all the sequences that were present in more than +one replicate. It provides the concidence type, clonaltype, sequence name, +replicate number, and the nucleotide sequence</span></p> + +<p class=MsoNormal> </p> + +</div> + +</body> + +</html> +</div></html>
